{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T04:41:24Z","timestamp":1694148084176},"reference-count":56,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,9,29]],"date-time":"2009-09-29T00:00:00Z","timestamp":1254182400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Jos\u00e9 Carreras Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:p>Re\u2010acquisition of immunocompetence after allogeneic bone marrow cell (BMC) transplantation depends on intrathymic maturation of the allogeneic T progenitor cells. We recently reported that CD44 promotes progenitor homing into the thymus and T\u2010cell maturation and now elucidate the molecular mechanisms of CD44\u2010supported thymocyte maturation. Lethally irradiated, tumor\u2010bearing mice, allogeneically reconstituted with T\u2010cell\u2010depleted BMC and a small number of common lymphoid progenitor 2 cells (CLP2) from transgenic (TG) mice, that express ratCD44v4\u2010v7 under the Thy1 promoter, showed accelerated immunocompetent T\u2010cell recovery compared with mice reconstituted with non\u2010transgenic (NTG) CLP2. In addition, graft\u2010versus\u2010host disease was strongly reduced after tumor vaccination. TG, but not NTG double\u2010negative (DN) thymocytes showed high proliferative potential, accompanied by constitutive association of lck with CD44. Importantly, when thymocyte adhesion was strengthened by anti\u2010CD44, co\u2010cultures of DN thymocytes with thymic stroma supported DN thymocyte maturation. The close contact between DN thymocytes and thymic stroma promoted persisting activation of lck and ERK1\/2, particularly in CD44v6<jats:sup>+<\/jats:sup> DN thymocytes. Thus, intrathymic T\u2010cell maturation in allogeneically reconstituted, leukemia\u2010bearing hosts can be considerably accelerated by high CD44v6 expression in early thymocytes, in which proliferation\u2010supporting signals are initiated by a crosstalk between CD44v6 on thymocytes and panCD44 on the thymic stroma.<\/jats:p>","DOI":"10.1038\/icb.2009.70","type":"journal-article","created":{"date-parts":[[2009,9,29]],"date-time":"2009-09-29T10:22:40Z","timestamp":1254219760000},"page":"136-147","source":"Crossref","is-referenced-by-count":2,"title":["Thymocyte expansion and maturation: crosstalk of CD44v6 on thymocytes and panCD44 on stroma cells"],"prefix":"10.1111","volume":"88","author":[{"given":"Mohini","family":"Rajasagi","sequence":"first","affiliation":[{"name":"Department of Tumor Cell Biology, University Hospital of Surgery and German Cancer Research Center  Heidelberg 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