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CD27 is constitutively expressed on NK cells and its expression correlates with sequential phases in NK\u2010cell development, discriminating phenotypically and functionally different subsets within the NK\u2010cell population. Although CD27 has been described to have an important regulatory role in effector and memory T and B lymphocytes, its role in NK\u2010cell biology remains to be addressed. In this study, we used CD27<jats:sup>\u2212\/\u2212<\/jats:sup> mice to investigate the role of CD27 in NK\u2010cell development and function, both during the resting state and upon stimulation. The results show that NK\u2010cell numbers are not impaired in CD27<jats:sup>\u2212\/\u2212<\/jats:sup> mice. Moreover, CD27<jats:sup>\u2212\/\u2212<\/jats:sup> NK cells reach full phenotypic maturity, evidenced by normal expression of CD49b, CD43 and CD11b. Expression of activating receptors is unaltered, whereas expression of several inhibitory receptors is increased. Cytotoxicity and interferon\u2010\u03b3 production by NK cells from CD27<jats:sup>\u2212\/\u2212<\/jats:sup> mice in the resting state are normal. However, upon <jats:italic>in vivo<\/jats:italic> anti\u2010CD40\u2010 or poly\u2010I:C\u2010mediated activation, or <jats:italic>in vitro<\/jats:italic> interleukin\u201015 priming plus anti\u2010NKp46 stimulation, the absence of CD27 results in decreased cytolytic activity and cytokine production by spleen and liver NK cells. In conclusion, this study demonstrates that CD27 is dispensable for the development of functional NK cells. However, upon stimulation of NK cells, CD27 displays an important role in their activation and functionality.<\/jats:p>","DOI":"10.1038\/icb.2010.171","type":"journal-article","created":{"date-parts":[[2011,2,1]],"date-time":"2011-02-01T10:39:58Z","timestamp":1296556798000},"page":"803-811","source":"Crossref","is-referenced-by-count":25,"title":["CD27\u2010deficient mice show normal NK\u2010cell differentiation but impaired function upon stimulation"],"prefix":"10.1111","volume":"89","author":[{"given":"Veerle","family":"De Colvenaer","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry, Microbiology and Immunology, Ghent University  Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvie","family":"Taveirne","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, Microbiology and Immunology, Ghent University  Ghent 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