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Using a rat model of asthma, we previously found that mucosal mast cells, which localise to the epithelial layer of the airways, are activated to promote a pro\u2010asthmatic immune response. In this study, we developed a neonatal rat model of allergic airway hypersensitivity that mimics some features of childhood asthma. Airway hypersensitivity was measured using unrestrained whole\u2010body plethysmography after analysis of the serum IgE titre. Inflammatory cells and inflammatory mediators in bronchoalveolar lavage fluid samples were examined. Two mast cell\u2010specific proteases were detected using PCR. In addition, we analysed the phenotype and the number of mast cells in the airways by immunohistochemistry, and we found that the number of mucosal mast cells and the expression level of the proteases increased 2 weeks after sensitisation. Changes in the IgE titre, airway hypersensitivity and the activation of other inflammatory cells were delayed, appearing during the 4 weeks after sensitisation. Our results indicate that the activation of mucosal mast cells contributes to the pro\u2010asthmatic immune response. This activation may be a biomarker allowing early intervention that could help prevent allergic airway inflammation.<\/jats:p>","DOI":"10.1038\/icb.2010.90","type":"journal-article","created":{"date-parts":[[2010,7,27]],"date-time":"2010-07-27T09:54:58Z","timestamp":1280224498000},"page":"239-245","source":"Crossref","is-referenced-by-count":2,"title":["Early activation of mucosal mast cells during the primary immune response in a rodent model of neonatal asthma"],"prefix":"10.1111","volume":"89","author":[{"given":"Shuang","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Information Biomedicine, Ehime University School of Medicine  Ehime Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masachika","family":"Shudou","sequence":"additional","affiliation":[{"name":"Department of Bioscience, Integrated Center for Sciences, Ehime University  Ehime Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazutaka","family":"Maeyama","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Information Biomedicine, Ehime University School of Medicine  Ehime Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,7,27]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mcp.0000148663.53798.72"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2003.10.034"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1500"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1997.77.4.1033"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2006.02.039"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2005.10.067"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.7.3855"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2003000700001"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-005-1346-9"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.61.3.233"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.11.2.259"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(00)90055-1"},{"key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.4049\/jimmunol.140.6.1953","article-title":"Generation of leukotriene C4, leukotriene B4, and prostaglandin D2 by immunologically activated rat intestinal mucosa mast cells","volume":"140","author":"Heavey DJ","year":"1988","journal-title":"J Immunol"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2005.03.001"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.118.4.1142"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.5.1299"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.12.4464"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1053\/rmed.2001.1174"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3038.2004.00128.x"},{"key":"e_1_2_6_20_1","first-page":"233","article-title":"Prevention of asthma by ketorifen in infants with atopic dermatits","volume":"68","author":"Iikura Y","year":"1992","journal-title":"Ann Allergy"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114507803412"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.156.3.9606031"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.86.38"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2004.01974.x"}],"container-title":["Immunology &amp; 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