{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:38:15Z","timestamp":1788871095430,"version":"build-2803163510"},"reference-count":11,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,2,4]],"date-time":"2014-02-04T00:00:00Z","timestamp":1391472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NHMRC-Program","award":["510488"],"award-info":[{"award-number":["510488"]}]},{"name":"NHMRC Practitioner Fellowship"},{"DOI":"10.13039\/501100001027","name":"Australian Government Department of Health and Ageing","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001027","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:p>\n                    The OX40\/CD25 assay is a novel technique that assesses antigen\u2010specific CD4\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell responses. To unequivocally demonstrate that responding cells are memory cells that become activated after secondary stimulation, na\u00efve CD45RA\n                    <jats:sup>+<\/jats:sup>\n                    and memory CD45RO\n                    <jats:sup>+<\/jats:sup>\n                    populations were stimulated with cytomegalovirus (CMV) lysate and the combined expression of CD25 and OX40 measured. As expected, the na\u00efve population showed very little response, whereas there was a higher response from the memory counterpart. To further elucidate CD4\n                    <jats:sup>+<\/jats:sup>\n                    memory T\u2010cell subsets involved in recall responses, CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells were separated into central memory (Tcm) and effector memory (Tem) subsets and stimulated with antigen\u2010pulsed antigen\u2010presenting cells (APCs). CMV responses in healthy donors showed a Tem\u2010dominant response with a Tem\/Tcm ratio of 1.2, whereas the tetanus toxoid responses were dominated by a Tcm response with a Tem\/Tcm ratio of 0.35. To determine memory response in the chronic of HIV infection, patient samples were used. A similar pattern to healthy donors was observed in seven chronic HIV+ patients at week 4 after anti\u2010retroviral therapy who responded to CMV with a larger response coming from Tem. The pattern was similar after 48 weeks of therapy but the responses were lower in magnitude. In chronic HIV+ patients who respond to Gag peptides, following institution of therapy there was an inversion of the ratio of the responding memory subsets compared with week 4, with a greater response from Tcm at week 48. This result was concordant with reduction in antigen load. As immune activation decreased there was also a decrease in the percentage of responding effector memory cells and maintenance of long\u2010term central memory.\n                  <\/jats:p>","DOI":"10.1038\/icb.2013.101","type":"journal-article","created":{"date-parts":[[2014,2,4]],"date-time":"2014-02-04T05:42:44Z","timestamp":1391492564000},"page":"384-388","source":"Crossref","is-referenced-by-count":12,"title":["Ratios of effector to central memory antigen\u2010specific CD4\n                    <sup>+<\/sup>\n                    T cells vary with antigen exposure in HIV+ patients"],"prefix":"10.1111","volume":"92","author":[{"given":"Chansavath","family":"Phetsouphanh","sequence":"first","affiliation":[{"name":"Kirby Institute, University of New South Wales Sydney New South Wales Australia"},{"name":"Centre for Applied Medical Research, St Vincent's Hospital Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Xu","sequence":"additional","affiliation":[{"name":"Kirby Institute, University of New South Wales Sydney New South Wales Australia"},{"name":"Centre for Applied Medical Research, St Vincent's Hospital Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Bailey","sequence":"additional","affiliation":[{"name":"Kirby Institute, University of New South Wales Sydney New South Wales Australia"},{"name":"Centre for Applied Medical Research, St Vincent's Hospital Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Pett","sequence":"additional","affiliation":[{"name":"Centre for Applied Medical Research, St Vincent's Hospital Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Zaunders","sequence":"additional","affiliation":[{"name":"Kirby Institute, University of New South Wales Sydney New South Wales Australia"},{"name":"Centre for Applied Medical Research, St Vincent's Hospital Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nabila","family":"Seddiki","sequence":"additional","affiliation":[{"name":"Kirby Institute, University of New South Wales Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony D","family":"Kelleher","sequence":"additional","affiliation":[{"name":"Kirby Institute, University of New South Wales Sydney New South Wales Australia"},{"name":"Centre for Applied Medical Research, St Vincent's Hospital Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,2,4]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.22.012703.104527"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.2.926"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.2.1037"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/44385"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2038"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200939722"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1046-2023(03)00124-5"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(01)00491-4"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803548"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1212245109"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1204836109"}],"container-title":["Immunology &amp; Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.2013.101","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.2013.101","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1038\/icb.2013.101","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.2013.101","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T12:19:03Z","timestamp":1694002743000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.2013.101"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,2,4]]},"references-count":11,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["10.1038\/icb.2013.101"],"URL":"https:\/\/doi.org\/10.1038\/icb.2013.101","archive":["Portico"],"relation":{},"ISSN":["0818-9641","1440-1711"],"issn-type":[{"value":"0818-9641","type":"print"},{"value":"1440-1711","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,2,4]]}}}