{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:55:43Z","timestamp":1791255343272,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,6,25]],"date-time":"2013-06-25T00:00:00Z","timestamp":1372118400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001511","name":"Auckland Medical Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001511","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:p><jats:italic>Staphylococcus aureus<\/jats:italic> is an opportunistic bacterial pathogen responsible for a range of diseases, from local skin infections through to life\u2010threatening illnesses such as toxic shock syndrome. <jats:italic>S. aureus<\/jats:italic> produces an assortment of molecules designed to evade or subvert the host immune system. One example is the 23\u2009kDa staphylococcal superantigen\u2010like protein 7 (SSL7) that simultaneously binds immunoglobulin A (IgA) and complement C5 to inhibit complement\u2010mediated hemolytic and bactericidal activity. The avirulent bacterium <jats:italic>Lactococcus lactis<\/jats:italic> was engineered to express SSL7 so that its role in bacterial survival could be assessed without interference from other virulence factors. Expression of SSL7 by <jats:italic>L. lactis<\/jats:italic> led to significantly enhanced bacterial survival in whole human blood and prevented the membrane attack complex (C5b\u20109) forming on the cell wall. To further understand the mechanism of action of SSL7, the activity of wild\u2010type SSL7 protein was compared with a panel of mutant proteins lacking the capacity to bind IgA, C5, or both IgA and C5. SSL7 potently inhibited <jats:italic>in vitro<\/jats:italic> chemotaxis of inflammatory myeloid cells in response to a pathogenic stimulus and when injected into mice, SSL7 blocked the migration of neutrophils into the peritoneum in response to an inoculum of heat\u2010killed <jats:italic>S. aureus<\/jats:italic>. Mutagenesis of the C5\u2010binding site on SSL7 abolished all inhibitory activity, while mutation of the IgA\u2010binding site had only partial effects, indicating that while IgA binding enhances activity it is not essential. SSL7 is an important staphylococcal virulence factor with potent anti\u2010inflammatory properties, which are mediated by targeting complement C5 and IgA.<\/jats:p>","DOI":"10.1038\/icb.2013.28","type":"journal-article","created":{"date-parts":[[2013,6,25]],"date-time":"2013-06-25T10:14:34Z","timestamp":1372155274000},"page":"469-476","source":"Crossref","is-referenced-by-count":27,"title":["Full functional activity of SSL7 requires binding of both complement C5 and IgA"],"prefix":"10.1111","volume":"91","author":[{"given":"Natalie","family":"Lorenz","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences, School of Medical Sciences and Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland Auckland New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona","family":"Clow","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences, School of Medical Sciences and Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland Auckland New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona J","family":"Radcliff","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences, School of Medical Sciences and Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland Auckland New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D","family":"Fraser","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences, School of Medical Sciences and Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland Auckland New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,6,25]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1017\/S002217240004609X"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1086\/533591"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/18.Supplement_A.1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2009.04.027"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01191.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2006.06.011"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1235"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20031636"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00709.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v30.i2.40"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.5.2926"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2009.09.027"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903311"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00764-12"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-06-015461"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.09.091"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05989.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-012-0926-8"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00399-12"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07720.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2007.03.001"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0706028104"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0910565107"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2010.01486.x"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2007.08.013"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.2174\/156652310790945557"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1824"},{"key":"e_1_2_8_28_1","doi-asserted-by":"crossref","first-page":"845","DOI":"10.4049\/jimmunol.130.2.845","article-title":"Studies on the mechanism of bacterial resistance to complement\u2010mediated killing. 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