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Here, we demonstrate preferential selection of CD8<jats:sup>+<\/jats:sup> thymocytes from <jats:italic>in vitro<\/jats:italic> differentiation of CD4<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> double\u2010positive thymocytes exhibiting the characteristics of nonconventional innate memory CD8<jats:sup>+<\/jats:sup> cells. In contrast to conventional CD8<jats:sup>+<\/jats:sup> thymocytes, these culture\u2010differentiated CD8<jats:sup>+<\/jats:sup> cells are eomesodermin positive and robustly express CXCR3, CD44, CD122 and TLR2. Interestingly, the pathogen\u2010associated molecule porin promotes preferential differentiation of the CD8<jats:sup>+<\/jats:sup> single\u2010positive subset in association with promyelocytic leukemia zinc\u2010finger upregulation and interleukin (IL)\u20104 production. On priming with anti\u2010CD3 antibody, porin augmented TLR2 and IFN\u2010\u03b3 indicating a role of the TLR ligand in acquisition of innate memory response of CD8<jats:sup>+<\/jats:sup> thymocytes. In addition, porin has a cooperative role with IL\u201015 on the expansion of memory\u2010phenotype CD8<jats:sup>+<\/jats:sup> T cells along with its effector function. Thus, the study opens an avenue to unfold the cues for development of these cells and the strategies adopted for bolstering immunity during primary infection.<\/jats:p>","DOI":"10.1038\/icb.2013.94","type":"journal-article","created":{"date-parts":[[2013,12,10]],"date-time":"2013-12-10T10:33:32Z","timestamp":1386671612000},"page":"368-376","source":"Crossref","is-referenced-by-count":5,"title":["Culture\u2010differentiated CD8<sup>+<\/sup> T cells acquire innate memory\u2010like traits and respond to a pathogen\u2010associated molecule"],"prefix":"10.1111","volume":"92","author":[{"given":"Ratna","family":"Biswas","sequence":"first","affiliation":[{"name":"Division of Immunology, National Institute of Cholera and Enteric Diseases Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Subhadeep","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Division of Immunology, National Institute of Cholera and Enteric Diseases Kolkata 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