{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:51:56Z","timestamp":1788871916647,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"FWO-Vlaanderen, Belgium"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:p>\n                    Although implantation of cellular material in the central nervous system (CNS) is a key direction in CNS regenerative medicine, this approach is currently limited by the occurrence of strong endogenous immune cell responses. In a model of mesenchymal stem cell (MSC) grafting in the CNS of immune\u2010competent mice, we previously described that MSC grafts become highly surrounded and invaded by Iba1\n                    <jats:sup>+<\/jats:sup>\n                    myeloid cells (microglia and\/or macrophages). Here, following grafting of blue fluorescent protein (BFP)\u2010expressing MSC in the CNS of CX\n                    <jats:sub>3<\/jats:sub>\n                    CR1\n                    <jats:sup>+\/\u2212<\/jats:sup>\n                    and CX\n                    <jats:sub>3<\/jats:sub>\n                    CR1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice, our results indicate: (1) that the observed inflammatory response is independent of the fractalkine signalling axis, and (2) that a significant spatial distribution of Iba1\n                    <jats:sup>+<\/jats:sup>\n                    inflammatory cells occurs, in which Iba1\n                    <jats:sup>+<\/jats:sup>\n                    CX\n                    <jats:sub>3<\/jats:sub>\n                    CR1\n                    <jats:sup>+<\/jats:sup>\n                    myeloid cells mainly surround the MSC graft and Iba1\n                    <jats:sup>+<\/jats:sup>\n                    CX\n                    <jats:sub>3<\/jats:sub>\n                    CR1\n                    <jats:sup>\u2212<\/jats:sup>\n                    myeloid cells mainly invade the graft at 10 days post transplantation. Although Iba1\n                    <jats:sup>+<\/jats:sup>\n                    CX\n                    <jats:sub>3<\/jats:sub>\n                    CR1\n                    <jats:sup>+<\/jats:sup>\n                    myeloid cells are considered to be of resident microglial origin, Iba1\n                    <jats:sup>+<\/jats:sup>\n                    CX\n                    <jats:sub>3<\/jats:sub>\n                    CR1\n                    <jats:sup>\u2212<\/jats:sup>\n                    myeloid cells are most likely of peripheral monocyte\/macrophage origin. In order to confirm the latter, we performed MSC\u2010BFP grafting experiments in the CNS of eGFP\n                    <jats:sup>+<\/jats:sup>\n                    bone marrow chimeric C57BL\/6 mice. Analysis of MSC\u2010BFP grafts in the CNS of these mice confirmed our observation that peripheral monocytes\/macrophages invade the MSC graft and that resident microglia surround the MSC graft site. Furthermore, analysis of major histocompatibility complex class II (MHCII) expression revealed that mainly macrophages, but not microglia, express this M1 pro\u2010inflammatory marker in the context of MSC grafting in the CNS. These results again highlight the complexity of cell implantation immunology in the CNS.\n                  <\/jats:p>","DOI":"10.1038\/icb.2014.49","type":"journal-article","created":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T09:05:21Z","timestamp":1404205521000},"page":"650-658","source":"Crossref","is-referenced-by-count":26,"title":["Distinct spatial distribution of microglia and macrophages following mesenchymal stem cell implantation in mouse brain"],"prefix":"10.1111","volume":"92","author":[{"given":"Debbie","family":"Le Blon","sequence":"first","affiliation":[{"name":"Experimental Cell Transplantation Group, Laboratory of Experimental Hematology, University of Antwerp Antwerp Belgium"},{"name":"Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chlo\u00e9","family":"Hoornaert","sequence":"additional","affiliation":[{"name":"Experimental Cell Transplantation Group, Laboratory of Experimental Hematology, University of Antwerp Antwerp Belgium"},{"name":"Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmijn","family":"Daans","sequence":"additional","affiliation":[{"name":"Experimental Cell Transplantation Group, Laboratory of Experimental Hematology, University of Antwerp Antwerp Belgium"},{"name":"Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Santermans","sequence":"additional","affiliation":[{"name":"Center for Statistics, I\u2010Biostat, Hasselt University Diepenbeek Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niel","family":"Hens","sequence":"additional","affiliation":[{"name":"Center for Statistics, I\u2010Biostat, Hasselt University Diepenbeek Belgium"},{"name":"Centre for Health Economic Research and Modeling Infectious Diseases (Chermid), University of Antwerp Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herman","family":"Goossens","sequence":"additional","affiliation":[{"name":"Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zwi","family":"Berneman","sequence":"additional","affiliation":[{"name":"Experimental Cell Transplantation Group, Laboratory of Experimental Hematology, University of Antwerp Antwerp Belgium"},{"name":"Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Ponsaerts","sequence":"additional","affiliation":[{"name":"Experimental Cell Transplantation Group, Laboratory of Experimental Hematology, University of Antwerp Antwerp Belgium"},{"name":"Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,7]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/sc.2008.13"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2010.141"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/scrt312"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2217\/rme.12.5"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2012.08.266"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11307-011-0469-3"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368912X636920"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.11.4106-4114.2000"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10645"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10037"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)00583-3"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0608385"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-04-348946"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1715"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.254268"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.05-5465com"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22474"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2114-11.2011"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.34351"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2923"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09615"},{"key":"e_1_2_7_22_1","unstructured":"CostaR BergwerfI SantermansE De VochtN PraetJ DaansJet al.Distinctin vitroproperties of embryonic and extra?embryonic fibroblast?like cells are reflected in theirin vivobehaviour following grafting in the adult mouse brain.Cell Transplant (e\u2010pub ahead of print 30 December 2013; doi:10.3727\/096368913X676196)."},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(85)90215-5"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-12-05197.2003"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1100421"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1000113"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2887"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4886(03)00082-7"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.6209-10.2011"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awp144"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2877"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058544"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2013.00026"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1174"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302094"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.778"},{"key":"e_1_2_7_37_1","first-page":"1","article-title":"Quantitative evaluation of stem cell grafting in the central nervous system of mice by in vivo bioluminescence imaging and postmortem multicolor histological analysis","volume":"1052","author":"Reekmans K","year":"2013","journal-title":"Methods Mol Biol"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/scrt147"}],"container-title":["Immunology &amp; Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.2014.49","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.2014.49","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1038\/icb.2014.49","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.2014.49","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T12:29:20Z","timestamp":1694003360000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.2014.49"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7]]},"references-count":38,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2014,9]]}},"alternative-id":["10.1038\/icb.2014.49"],"URL":"https:\/\/doi.org\/10.1038\/icb.2014.49","archive":["Portico"],"relation":{},"ISSN":["0818-9641","1440-1711"],"issn-type":[{"value":"0818-9641","type":"print"},{"value":"1440-1711","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7]]}}}