{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T09:06:05Z","timestamp":1778663165431,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,7,22]],"date-time":"2014-07-22T00:00:00Z","timestamp":1405987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT PRH 00078"],"award-info":[{"award-number":["PICT PRH 00078"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP 0101"],"award-info":[{"award-number":["PIP 0101"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005363","name":"Universidad de Buenos Aires","doi-asserted-by":"publisher","award":["20020110200026"],"award-info":[{"award-number":["20020110200026"]}],"id":[{"id":"10.13039\/501100005363","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:p>Human B\u2010cell studies <jats:italic>in vitro<\/jats:italic> have routinely used B lymphocytes purified from spleen, blood or tonsils irrespective of potential differences in their immunological traits. In this study, we compared the functional responses of total (CD19<jats:sup>+<\/jats:sup>) and memory B cells (B<jats:sub>mem<\/jats:sub>; CD19<jats:sup>+<\/jats:sup>\/CD27<jats:sup>+<\/jats:sup>) isolated from blood and tonsils to different stimuli. Peripheral B cells showed enhanced survival and proliferation compared with their tonsillar equivalents when stimulated for 10 days. Stimulated B cells from both tissues secreted significantly greater amounts of cytokines than unstimulated controls demonstrating their functional responsiveness. Analysis of CD27 expression over time indicated that the conditions that promoted survival and proliferation of peripheral B<jats:sub>mem<\/jats:sub>, caused massive tonsillar B<jats:sub>mem<\/jats:sub> death. Purified tonsillar B<jats:sub>mem<\/jats:sub> failed to expand but rapidly differentiated in antibody secreting cells and subsequently underwent apoptosis. In contrast, circulating B<jats:sub>mem<\/jats:sub> showed delayed activation and differentiation, but exhibited a longer lifespan and active proliferation. In addition, short\u2010term stimulation of tonsillar B<jats:sub>mem<\/jats:sub> resulted in the production of more immunoglobulin G (IgG) than their peripheral counterparts. At later time points, however, IgG production from the different B cells was reversed. Our findings imply that the tissue located and peripheral B<jats:sub>mem<\/jats:sub> have distinct behaviors, indicating organ dependent functional responses that should not be generalizable to all B<jats:sub>mem<\/jats:sub>. This work provides a greater understanding of how B<jats:sub>mem<\/jats:sub> location is coupled to specialized roles of B lymphocytes.<\/jats:p>","DOI":"10.1038\/icb.2014.59","type":"journal-article","created":{"date-parts":[[2014,7,22]],"date-time":"2014-07-22T09:48:56Z","timestamp":1406022536000},"page":"882-887","source":"Crossref","is-referenced-by-count":22,"title":["Human memory B cells isolated from blood and tonsils are functionally distinctive"],"prefix":"10.1111","volume":"92","author":[{"given":"M Eugenia","family":"P\u00e9rez","sequence":"first","affiliation":[{"name":"Institute of Immunology, Genetics and Metabolism (INIGEM), Immunogenetics Department, Clinical Hospital, University of Buenos Aires, National Council for Scientific and Technological Research Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Ariel","family":"Billordo","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Genetics and Metabolism (INIGEM), Immunogenetics Department, Clinical Hospital, University of Buenos Aires, National Council for Scientific and Technological Research Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pl\u00e1cida","family":"Baz","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Genetics and Metabolism (INIGEM), Immunogenetics Department, Clinical Hospital, University of Buenos Aires, National Council for Scientific and Technological Research Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo","family":"Fainboim","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Genetics and Metabolism (INIGEM), Immunogenetics Department, Clinical Hospital, University of Buenos Aires, National Council for Scientific and Technological Research Buenos Aires Argentina"},{"name":"Department of Immunology, School of Medicine, University of Buenos Aires Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eloisa","family":"Arana","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Genetics and Metabolism (INIGEM), Immunogenetics Department, Clinical Hospital, University of Buenos Aires, National Council for Scientific and Technological Research Buenos Aires Argentina"},{"name":"Department of Immunology, School of Medicine, University of Buenos Aires Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,7,22]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201011-1783OC"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2005.00283.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64841-9"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.12.040194.001001"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830270835"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.9.1679"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.9.1691"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00318-7"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-008-9267-3"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.10.008"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/44385"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni889"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201343751"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.12.2209"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.2.686"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.10.5244"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-812-9:031"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.1.261"},{"key":"e_1_2_7_19_1","doi-asserted-by":"crossref","first-page":"2597","DOI":"10.4049\/jimmunol.130.6.2597","article-title":"The role of B cell proliferation in the generation of immunoglobulin\u2010secreting cells in man","volume":"130","author":"Jelinek DF","year":"1983","journal-title":"J Immunol"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.6.1817"},{"key":"e_1_2_7_21_1","first-page":"326","article-title":"Comparison of immunological characteristics of peripheral, splenic and tonsilar naive B cells by differential gene expression meta\u2010analyses","volume":"30","author":"Chokeshai\u2010u\u2010saha K","year":"2012","journal-title":"Asian Pac J Allergy Immunol"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02630-07"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02675-09"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.159.5.2085"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1076071"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1214100"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5258.54"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00339-4"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2007.00540.x"}],"container-title":["Immunology &amp; Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.2014.59","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.2014.59","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1038\/icb.2014.59","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.2014.59","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T23:23:59Z","timestamp":1694042639000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.2014.59"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,22]]},"references-count":29,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2014,11]]}},"alternative-id":["10.1038\/icb.2014.59"],"URL":"https:\/\/doi.org\/10.1038\/icb.2014.59","archive":["Portico"],"relation":{},"ISSN":["0818-9641","1440-1711"],"issn-type":[{"value":"0818-9641","type":"print"},{"value":"1440-1711","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,22]]}}}