{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T10:45:18Z","timestamp":1774521918140,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R15AI101920"],"award-info":[{"award-number":["1R15AI101920"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:p>Genital chlamydial infections lead to severe upper reproductive tract pathology in a subset of untreated women. We demonstrated previously that tumor necrosis factor (TNF)\u2010\u03b1\u2010producing CD8<jats:sup>+<\/jats:sup> T cells contribute significantly to chlamydial upper genital tract pathology in female mice. In addition, we observed that minimal chlamydial oviduct pathology develops in OT\u20101 transgenic (OT\u20101) mice, wherein the CD8<jats:sup>+<\/jats:sup> T\u2010cell repertoire is restricted to recognition of the ovalbumin peptide Ova<jats:sub>257\u2013264<\/jats:sub>, suggesting that non\u2010<jats:italic>Chlamydia<\/jats:italic>\u2010specific CD8<jats:sup>+<\/jats:sup> T cells may not be responsible for chlamydial pathogenesis. In the current study, we evaluated whether antigen\u2010specific CD8<jats:sup>+<\/jats:sup> T cells mediate chlamydial pathology. Groups of wild\u2010type (WT) C57BL\/6J, OT\u20101 mice, and OT\u20101 mice replete with WT CD8<jats:sup>+<\/jats:sup> T cells (1 \u00d7 10<jats:sup>6<\/jats:sup> cells per mouse intravenously) were infected intravaginally with <jats:italic>C. muridarum<\/jats:italic> (5 \u00d7 10<jats:sup>4<\/jats:sup> IFU\/mouse). Serum total anti\u2010<jats:italic>Chlamydia<\/jats:italic> antibody and total splenic anti\u2010<jats:italic>Chlamydia<\/jats:italic> interferon (IFN)\u2010\u03b3 and TNF\u2010\u03b1 responses were comparable among the three groups of animals. However, <jats:italic>Chlamydia<\/jats:italic>\u2010specific IFN\u2010\u03b3 and TNF\u2010\u03b1 production from purified splenic CD8<jats:sup>+<\/jats:sup> T cells of OT\u20101 mice was minimal, whereas responses in OT\u20101 mice replete with WT CD8<jats:sup>+<\/jats:sup> T cells were comparable to those in WT animals. Vaginal chlamydial clearance was comparable between the three groups of mice. Importantly, the incidence and severity of oviduct and uterine horn pathology was significantly reduced in OT\u20101 mice but reverted to WT levels in OT\u20101 mice replete with WT CD8<jats:sup>+<\/jats:sup> T cells. Collectively, these results demonstrate that <jats:italic>Chlamydia<\/jats:italic>\u2010specific CD8<jats:sup>+<\/jats:sup> T cells contribute significantly to upper genital tract pathology.<\/jats:p>","DOI":"10.1038\/icb.2015.74","type":"journal-article","created":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T10:26:38Z","timestamp":1441103198000},"page":"208-212","source":"Crossref","is-referenced-by-count":63,"title":["The contribution of <i>Chlamydia<\/i>\u2010specific CD8<sup>+<\/sup> T cells to upper genital tract pathology"],"prefix":"10.1111","volume":"94","author":[{"given":"Kelly R","family":"Vlcek","sequence":"first","affiliation":[{"name":"Department of Pathology, Midwestern University  Downers Grove IL USA"},{"name":"Department of Biomedical Sciences, Midwestern University  Downers Grove IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weidang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology, Midwestern University  Downers Grove IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srikanth","family":"Manam","sequence":"additional","affiliation":[{"name":"Department of Pathology, Midwestern University  Downers Grove IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Zanotti","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Midwestern University  Downers Grove IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce J","family":"Nicholson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Texas Health Science Center at San Antonio  San Antonio TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyle H","family":"Ramsey","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Midwestern University  Downers Grove IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashlesh K","family":"Murthy","sequence":"additional","affiliation":[{"name":"Department of Pathology, Midwestern University  Downers Grove IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,9]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2007.028142"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro931"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199207000-00001"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.bmb.a071806"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504198102"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(94)35133-3"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.6.2741-2751.2002"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05022-11"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/2049-632X.12032"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.12.6979-6987.2000"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1086\/605411"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/173.6.1437"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.65.6.2175-2182.1997"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/174.3.647"},{"key":"e_1_2_8_15_1","first-page":"311","volume-title":"Chlamydia Vaccine: Progress and Challenges","author":"Murthy AK","year":"2012"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.62.11.5195-5197.1994"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/813238"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1402675"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1400120"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01280-06"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu839"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.02.010"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0064917"}],"container-title":["Immunology &amp; Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.2015.74","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.2015.74","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1038\/icb.2015.74","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.2015.74","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T00:53:59Z","timestamp":1694048039000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.2015.74"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9]]},"references-count":23,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,2]]}},"alternative-id":["10.1038\/icb.2015.74"],"URL":"https:\/\/doi.org\/10.1038\/icb.2015.74","archive":["Portico"],"relation":{},"ISSN":["0818-9641","1440-1711"],"issn-type":[{"value":"0818-9641","type":"print"},{"value":"1440-1711","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9]]}}}