{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:40:43Z","timestamp":1790671243604,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,9,22]],"date-time":"2015-09-22T00:00:00Z","timestamp":1442880000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008392","name":"Sundhed og Sygdom, Det Frie Forskningsr\u00e5d","doi-asserted-by":"publisher","award":["09\u2010067407"],"award-info":[{"award-number":["09\u2010067407"]}],"id":[{"id":"10.13039\/100008392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:p>Chlamydia is the most widespread sexually transmitted bacterial disease and a prophylactic vaccine is highly needed. Ideally, this vaccine is required to induce a combined response of Th1 cell\u2010mediated immune (CMI) response in concert with neutralizing antibodies. Using a novel G\u00f6ttingen minipig animal model, we evaluated the immunogenicity and efficacy of a multi\u2010subunit vaccine formulated in the strong Th1\u2010inducing adjuvant CAF01. We evaluated a mixture of two fusion proteins (Hirep1 and CTH93) designed to promote either neutralizing antibodies or cell\u2010mediated immunity, respectively. Hirep1 is a novel immunogen based on the variant domain (VD) 4 region from major outer membrane protein (MOMP) serovar (Sv) D, SvE and SvF, and CTH93 is a fusion molecule of three antigens (CT043, CT414 and MOMP). Pigs were immunized twice intramuscularly with either Hirep1+CTH93\/CAF01, UV\u2010inactivated <jats:italic>Chlamydia trachomatis<\/jats:italic> SvD bacteria (UV\u2010SvD\/CAF01) or CAF01. The Hirep1+CTH93\/CAF01 vaccine induced a strong CMI response against the vaccine antigens and high titers of antibodies, particularly against the VD4 region of MOMP. Sera from Hirep1+CTH93\/CAF01 immunized pigs neutralized <jats:italic>C. trachomatis<\/jats:italic> SvD and SvF infectivity <jats:italic>in vitro<\/jats:italic>. Both Hirep1+CTH93\/CAF01 and UV\u2010SvD\/CAF01 vaccination protected pigs against a vaginal <jats:italic>C. trachomatis<\/jats:italic> SvD infection. In conclusion, the Hirep1+CTH93\/CAF01 vaccine proved highly immunogenic and equally protective as UV\u2010SvD\/CAF01 showing promise for the development of a subunit vaccine against <jats:italic>Chlamydia<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/icb.2015.79","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T08:25:05Z","timestamp":1439454305000},"page":"185-195","source":"Crossref","is-referenced-by-count":48,"title":["A multi\u2010subunit <i>Chlamydia<\/i> vaccine inducing neutralizing antibodies and strong IFN\u2010\u03b3<sup>+<\/sup> CMI responses protects against a genital infection in minipigs"],"prefix":"10.1111","volume":"94","author":[{"given":"Sarah","family":"B\u00f8je","sequence":"first","affiliation":[{"name":"Section of Veterinary Reproduction and Obstetrics, Department of Large Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen  Frederiksberg C Denmark"},{"name":"Chlamydia Vaccine Research, Department 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