{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:28:42Z","timestamp":1791217722181,"version":"4.1.0"},"reference-count":63,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,12,8]],"date-time":"2015-12-08T00:00:00Z","timestamp":1449532800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:p><jats:italic>Salmonella enterica serovar<\/jats:italic> Typhimurium (ST) is a virulent intracellular bacterium that conceals itself in the phagosomes of infected cells. Although CD8<jats:sup>+<\/jats:sup> T cells promote protection against various intracellular pathogens, the role of CD8<jats:sup>+<\/jats:sup> T cells against virulent ST has been unclear due to early fatality of susceptible (B6) mice. Herein, we generated MHC I\u2010deficient mice on the resistant (129SvJ) and susceptible (Nramp1 transgenic B6) background to evaluate the role of CD8<jats:sup>+<\/jats:sup> T cells against virulent ST. Our results indicate that CD8<jats:sup>+<\/jats:sup> T cells have a critical protective role in host survival during infection with virulent ST. As antigen presentation and CD8<jats:sup>+<\/jats:sup> T\u2010cell activation against phagosomal antigens are considered to operate through the cross\u2010presentation pathway, we have evaluated CD8<jats:sup>+<\/jats:sup> T\u2010cell response against ST in Batf3\u2010deficient mice that lack CD8\u03b1 dendritic cells (DCs). Using a recombinant of ST that expresses antigen (ST\u2010OVA) mainly in the phagosomes of infected cells, we show that CD8<jats:sup>+<\/jats:sup> T\u2010cell response is compromised throughout the duration of infection in Batf3\u2010deficient mice. In contrast, when ST delivers antigen to the cytosol of infected cells (ST\u2010OVA\u2010C), CD8<jats:sup>+<\/jats:sup> T\u2010cell response against the cytosolic antigen was compromised only in the short term in the absence of CD8\u03b1 DCs, with wild\u2010type and Batf3\u2010deficient mice generating similar CD8<jats:sup>+<\/jats:sup> T\u2010cell response in the long term. Thus, Batf3 has an important role in CD8<jats:sup>+<\/jats:sup> T\u2010cell priming regardless of antigenic location; however, its role is redundant at later time intervals against cytosolic antigen.<\/jats:p>","DOI":"10.1038\/icb.2015.98","type":"journal-article","created":{"date-parts":[[2015,11,13]],"date-time":"2015-11-13T09:21:35Z","timestamp":1447406495000},"page":"378-387","source":"Crossref","is-referenced-by-count":11,"title":["Transcription factor Batf3 is important for development of CD8<sup>+<\/sup> T\u2010cell response against a phagosomal bacterium regardless of the location of antigen"],"prefix":"10.1111","volume":"94","author":[{"given":"Rajen","family":"Patel","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa  Ottawa ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subash","family":"Sad","sequence":"additional","affiliation":[{"name":"Department of 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