{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:26:03Z","timestamp":1740126363280,"version":"3.37.3"},"reference-count":65,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,1,24]],"date-time":"2017-01-24T00:00:00Z","timestamp":1485216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1RO3AI092621\u201001"],"award-info":[{"award-number":["1RO3AI092621\u201001"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:p>We have comprehensively demonstrated using the mouse model that intranasal immunization with recombinant chlamydial protease\u2010like activity factor (rCPAF) leads to a significant reduction in bacterial burden, genital tract pathology and preserves fertility following intravaginal genital chlamydial challenge. In the present report, we evaluated the protective efficacy of rCPAF immunization in guinea pigs, a second animal model for genital chlamydial infection. Using a vaccination strategy similar to the mouse model, we intranasally immunized female guinea pigs with rCPAF plus CpG deoxynucleotides (CpG; as an adjuvant), and challenged intravaginally with <jats:italic>C. trachomatis<\/jats:italic> serovar D (CT\u2010D). Immunization with rCPAF\/CpG significantly reduced vaginal CT\u2010D shedding and induced resolution of infection by day 24, compared with day 33 in CpG alone treated and challenged animals. Immunization induced robust anti\u2010rCPAF serum IgG 2 weeks following the last immunization, and was sustained at a high\u2010level 4 weeks post challenge. Upregulation of antigen\u2010specific <jats:italic>IFN\u2010\u03b3<\/jats:italic> gene expression was observed in rCPAF\/CpG\u2010vaccinated splenocytes. Importantly, a significant reduction in inflammation in the genital tissue in rCPAF\/CpG\u2010immunized guinea pigs compared with CpG\u2010immunized animals was observed. Taken together, this study provides evidence of the protective efficacy of rCPAF as a vaccine candidate in a second animal model of genital chlamydial infection.<\/jats:p>","DOI":"10.1038\/icb.2016.122","type":"journal-article","created":{"date-parts":[[2016,12,19]],"date-time":"2016-12-19T09:21:14Z","timestamp":1482139274000},"page":"454-460","source":"Crossref","is-referenced-by-count":3,"title":["Chlamydial protease\u2010like activity factor mediated protection against <i>C. trachomatis<\/i> in guinea pigs"],"prefix":"10.1111","volume":"95","author":[{"given":"Shradha","family":"Wali","sequence":"first","affiliation":[{"name":"South Texas Center for Emerging Infectious Diseases and Center for Excellence in Infection Genomics, University of Texas at San Antonio, One UTSA Circle  San Antonio TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rishein","family":"Gupta","sequence":"additional","affiliation":[{"name":"South Texas Center for Emerging Infectious 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