{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:06:25Z","timestamp":1791295585082,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,9,20]],"date-time":"2016-09-20T00:00:00Z","timestamp":1474329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BBG018839\/1"],"award-info":[{"award-number":["BBG018839\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:p>Infection and injury of the gut are associated with cell damage and release of molecules such as extracellular adenosine 5\u2032\u2010triphosphate (ATP), which is recognised by the purinergic P2X7 receptor (P2X7R). P2X7R is widely expressed in the gut by antigen\u2010presenting cells (APCs) and epithelial cells, but the role of the P2X7R on epithelial cells is poorly understood. We investigated P2X7R in intestinal epithelium<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>using two model infections,<jats:italic>Toxoplasma gondii<\/jats:italic>and<jats:italic>Trichinella spiralis<\/jats:italic>. Lipopolysaccharide and ATP treatment of intestinal epithelial cells and infection with<jats:italic>T. gondii in vitro<\/jats:italic>did not promote inflammasome\u2010associated interleukin\u20101\u03b2 (IL\u20101\u03b2) or IL\u201018 secretion, but promoted C\u2013C motif chemokine ligand 5 (CCL5), tumour necrosis factor\u2010\u03b1 and IL\u20106 production that were significantly reduced when the P2X7R was blocked. Similarly,<jats:italic>in vivo<\/jats:italic>, infection with either<jats:italic>T. spiralis<\/jats:italic>or<jats:italic>T. gondii<\/jats:italic>induced rapid upregulation of epithelial CCL5 in wild\u2010type (wild\u2010type (WT)) mice that was significantly reduced in P2X7R<jats:sup>\u2212\/\u2212<\/jats:sup>littermate controls. The effects of reduced epithelial CCL5 were assayed by investigating recruitment of dendritic cells (DCs) to the epithelium. Infection induced a rapid recruitment of CD11c<jats:sup>+<\/jats:sup>CD103<jats:sup>+<\/jats:sup>DC subsets into the epithelial layer of WT mice but not P2X7R<jats:sup>\u2212\/\u2212<\/jats:sup>mice.<jats:italic>In vitro<\/jats:italic>chemotaxis assays and bone marrow chimeras demonstrated the importance of epithelial P2X7R in DC recruitment. P2X7R signalling in epithelial cells mediates chemokine responses to promote initiation of host immunity to infection.<\/jats:p>","DOI":"10.1038\/icb.2016.75","type":"journal-article","created":{"date-parts":[[2016,8,25]],"date-time":"2016-08-25T08:21:19Z","timestamp":1472113279000},"page":"178-188","source":"Crossref","is-referenced-by-count":39,"title":["P2X7 receptor\u2010dependent tuning of gut epithelial responses to infection"],"prefix":"10.1111","volume":"95","author":[{"given":"Szu\u2010Wei","family":"Huang","sequence":"first","affiliation":[{"name":"Faculty of Biology, Medicine and Health, The University of Manchester, Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Walker","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Medicine and Health, The University of Manchester, Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanne","family":"Pennock","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Medicine and Health, The University of Manchester, Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn","family":"Else","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Medicine and Health, The University of Manchester, Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner","family":"Muller","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Medicine and Health, The University of Manchester, Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael JD","family":"Daniels","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Medicine and Health, The University of Manchester, Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Pellegrini","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Brough","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Medicine and Health, The University of Manchester, Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria","family":"Lopez\u2010Castejon","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Medicine and Health, The University of Manchester, Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheena M","family":"Cruickshank","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Medicine and Health, The University of Manchester, Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,9,20]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i41.15216"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0803124105"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.256pe6"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-011-8203-4"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.2011.578068"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.113.209452"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002212"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00282.2009"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1100423"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/gene.2010.31"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2010.03.004"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0802749"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200636199"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2002\/941087"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2013.125"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0802968"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M104388200"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2015.10.009"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000336628"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M006781200"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1301301"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1109893"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni915"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-10-19"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a016246"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.4149\/gpb_2012_048"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/292376"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903450"},{"key":"e_1_2_6_29_1","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/S1016-8478(23)15098-9","article-title":"Effects of 2,3,7,8\u2010tetrachlorodibenzo\u2010p\u2010dioxin on the expression of synaptic proteins in dissociated rat cortical cells","volume":"14","author":"Cho SJ","year":"2002","journal-title":"Mol Cells"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2010.05.013"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2009.00885.x"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1004036"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2011.08.008"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2008.39"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2011.01015.x"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.03.009"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003801"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000048472"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.1.309-316.2003"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0804307"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.06.015"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2014.183"}],"container-title":["Immunology &amp; Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.2016.75","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.2016.75","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1038\/icb.2016.75","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.2016.75","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T01:49:43Z","timestamp":1718761783000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.2016.75"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,20]]},"references-count":42,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,2]]}},"alternative-id":["10.1038\/icb.2016.75"],"URL":"https:\/\/doi.org\/10.1038\/icb.2016.75","archive":["Portico"],"relation":{},"ISSN":["0818-9641","1440-1711"],"issn-type":[{"value":"0818-9641","type":"print"},{"value":"1440-1711","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,20]]}}}