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It is clear from a quantitative review of the literature that intravital microscopy is a key tool in both historic and contemporary immunological research, providing unique advances in our understanding of immune responses. We have chosen to focus this review on how intravital microscopy methodologies are used to image specific organs or systems and we present recent descriptions of fundamental immunological processes that could not have been achieved by other methods. The following target organs\/systems are discussed in more detail: cremaster muscle, skin (ear and dorsal skin fold chamber), lymph node, liver, lung, mesenteric vessels, carotid artery, bone marrow, brain, spleen, foetus and lastly vessels of the knee joint.<\/jats:p>","DOI":"10.1038\/icb.2017.25","type":"journal-article","created":{"date-parts":[[2017,4,3]],"date-time":"2017-04-03T08:20:28Z","timestamp":1491207628000},"page":"506-513","source":"Crossref","is-referenced-by-count":65,"title":["Intravital microscopy in historic and contemporary immunology"],"prefix":"10.1111","volume":"95","author":[{"given":"Judith","family":"Secklehner","sequence":"first","affiliation":[{"name":"Cancer Research UK Beatson Institute, Garscube Campus  Glasgow UK"},{"name":"Inflammation, Repair &amp; Development, National Heart &amp; Lung Institute, Imperial College London, Sir Alexander Fleming Building, South Kensington  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Lo Celso","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Imperial College London, Sir Alexander Fleming Building, South Kensington  London UK"},{"name":"The Francis Crick Institute  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leo M","family":"Carlin","sequence":"additional","affiliation":[{"name":"Cancer Research UK Beatson Institute, Garscube Campus  Glasgow UK"},{"name":"Inflammation, Repair &amp; Development, National Heart &amp; Lung Institute, Imperial College London, Sir Alexander Fleming Building, South Kensington  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,4,25]]},"reference":[{"key":"e_1_2_18_1_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00016.2013"},{"key":"e_1_2_18_2_1","doi-asserted-by":"crossref","DOI":"10.5962\/bhl.title.5700","volume-title":"The select works of Anthony van Leeuwenhoek: containing his microscopical discoveries in many of the works of nature","author":"Leeuwenhoek A","year":"1800"},{"key":"e_1_2_18_3_1","volume-title":"Icones physiologicae; tabulae physiologiam et geneseos historiam illustrantes","author":"Wagner R","year":"1839"},{"key":"e_1_2_18_4_1","volume-title":"Lectures on the Comparative Pathology of Inflammation Delivered at the Pasteur Institute in 1891","author":"Metchnikoff E","year":"1893"},{"key":"e_1_2_18_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/1470412912455622"},{"key":"e_1_2_18_6_1","first-page":"330","volume":"28","author":"Heimstadt O","year":"1911","journal-title":"Das Fluoreszenzmikroskop. 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