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Colon digesta samples were collected on day 39 and subjected to denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene fragments. DGGE fingerprint diversity indices differed between the group treated with amoxicillin and the untreated group (0.8\u00b10.19 and 1.03\u00b10.17, respectively, P=0.012). Reamplification and sequencing of two bands present in all samples revealed that a Roseburia faecalis-related population was strongly reduced in relative abundance (98% identity) in the treated group, while an enterobacterial population with 100% identity to Shigella spp., Escherichia coli and Salmonella enterica serovar Typhi was enriched. A band corresponding to Lactobacillus sobrius was present only in the control group. The protective effect of prophylactic antibiotic administration may be outweighed by the long-lasting disturbance of the gut ecosystem.<\/jats:p>","DOI":"10.1038\/ismej.2007.29","type":"journal-article","created":{"date-parts":[[2007,6,1]],"date-time":"2007-06-01T08:02:47Z","timestamp":1180684967000},"page":"180-183","source":"Crossref","is-referenced-by-count":63,"title":["Parenteral long-acting amoxicillin reduces intestinal bacterial community diversity in piglets even 5 weeks after the administration"],"prefix":"10.1093","volume":"1","author":[{"given":"Pawel","family":"Janczyk","sequence":"first","affiliation":[{"name":"Research Unit for Nutritional Physiology \u2018Oskar Kellner\u2019, Research Institute for the Biology of Farm Animals , Dummerstorf , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Pieper","sequence":"additional","affiliation":[{"name":"Research Unit for Nutritional Physiology \u2018Oskar Kellner\u2019, Research 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