{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T08:49:49Z","timestamp":1791103789578,"version":"4.1.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2009,5,7]],"date-time":"2009-05-07T00:00:00Z","timestamp":1241654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In the plant-beneficial, root-colonizing strain Pseudomonas fluorescens CHA0, the Gac\/Rsm signal transduction pathway positively regulates the synthesis of biocontrol factors (mostly antifungal secondary metabolites) and contributes to oxidative stress response via the stress sigma factor RpoS. The backbone of this pathway consists of the GacS\/GacA two-component system, which activates the expression of three small regulatory RNAs (RsmX, RsmY, RsmZ) and thereby counters translational repression exerted by the RsmA and RsmE proteins on target mRNAs encoding biocontrol factors. We found that the expression of typical biocontrol factors, that is, antibiotic compounds and hydrogen cyanide (involving the phlA and hcnA genes), was significantly lower at 35\u2009\u00b0C than at 30\u2009\u00b0C. The expression of the rpoS gene was affected in parallel. This temperature control depended on RetS, a sensor kinase acting as an antagonist of the GacS\/GacA system. An additional sensor kinase, LadS, which activated the GacS\/GacA system, apparently did not contribute to thermosensitivity. Mutations in gacS or gacA were epistatic to (that is, they overruled) mutations in retS or ladS for expression of the small RNAs RsmXYZ. These data are consistent with a model according to which RetS\u2013GacS and LadS\u2013GacS interactions shape the output of the Gac\/Rsm pathway and the environmental temperature influences the RetS\u2013GacS interaction in P. fluorescens CHA0.<\/jats:p>","DOI":"10.1038\/ismej.2009.42","type":"journal-article","created":{"date-parts":[[2009,5,7]],"date-time":"2009-05-07T10:39:14Z","timestamp":1241692754000},"page":"955-965","source":"Crossref","is-referenced-by-count":77,"title":["Temperature-responsive sensing regulates biocontrol factor expression in <i>Pseudomonas fluorescens<\/i> CHA0"],"prefix":"10.1093","volume":"3","author":[{"given":"B\u00e9r\u00e9nice","family":"Humair","sequence":"first","affiliation":[{"name":"D\u00e9partement de Microbiologie Fondamentale, Universit\u00e9 de Lausanne , Lausanne , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"D\u00e9partement de 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