{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T13:51:25Z","timestamp":1758981085832},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2017,10,13]],"date-time":"2017-10-13T00:00:00Z","timestamp":1507852800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2017,10,13]],"date-time":"2017-10-13T00:00:00Z","timestamp":1507852800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The environmental resistome has been recognized as the origin and reservoir of antibiotic resistance genes and considered to be dynamic and ever expanding. In this study, a targeted gene sequencing approach revealed that the polymorphic diversity of the aminoglycoside-inactivating enzyme AAC(6\u2032)-Ib was ecological niche-specific. AAC(6\u2032)-Ib-cr, previously known as a clinical variant, was prevalent in various soils and the intestines of chickens and humans, suggesting that this variant might not have arisen from adaptive mutations in the clinic but instead originated from the environment. Furthermore, ecologically dominant polymorphic variants of AAC(6\u2032)-Ib were characterized and found to display different substrate specificities for quinolones and aminoglycosides, conferring the altered resistance spectra. Interestingly, a novel variant with the D179Y substitution showed an extended resistance spectrum to the recently developed fluoroquinolone gemifloxacin. Our results suggest that soil and animal microbiomes could be major reservoirs of antibiotic resistance; polymorphic diversity expands the antibiotic resistome in the environment, resulting in the potential emergence of novel resistance.<\/jats:p>","DOI":"10.1038\/ismej.2017.168","type":"journal-article","created":{"date-parts":[[2017,10,13]],"date-time":"2017-10-13T14:04:20Z","timestamp":1507903460000},"page":"267-276","update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Polymorphism of antibiotic-inactivating enzyme driven by ecology expands the environmental resistome"],"prefix":"10.1093","volume":"12","author":[{"given":"Dae-Wi","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Systems Biotechnology and Center for Antibiotic Resistome, Chung-Ang University , Anseong, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cung Nawl","family":"Thawng","sequence":"additional","affiliation":[{"name":"Department of Systems Biotechnology and Center for Antibiotic Resistome, Chung-Ang University , Anseong, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung-Hye","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Systems Biotechnology and Center for Antibiotic Resistome, Chung-Ang University , Anseong, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kihyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Systems Biotechnology and Center for Antibiotic Resistome, Chung-Ang University , Anseong, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang-Jun","family":"Cha","sequence":"additional","affiliation":[{"name":"Department of Systems Biotechnology and Center for Antibiotic Resistome, Chung-Ang University , Anseong, Republic of 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