{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T12:59:44Z","timestamp":1778504384774,"version":"3.51.4"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2014,8,6]],"date-time":"2014-08-06T00:00:00Z","timestamp":1407283200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Antibiot"],"published-print":{"date-parts":[[2015,2]]},"DOI":"10.1038\/ja.2014.100","type":"journal-article","created":{"date-parts":[[2014,8,6]],"date-time":"2014-08-06T12:04:50Z","timestamp":1407326690000},"page":"126-132","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Bafilomycin L, a new inhibitor of cholesteryl ester synthesis in mammalian cells, produced by marine-derived Streptomyces sp. OPMA00072"],"prefix":"10.1038","volume":"68","author":[{"given":"Keisuke","family":"Kobayashi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Fukuda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeo","family":"Usui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuko","family":"Kurihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiko","family":"Kanamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Tomoda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2014,8,6]]},"reference":[{"key":"BFja2014100_CR1","doi-asserted-by":"publisher","first-page":"1355","DOI":"10.1007\/s00018-006-6016-8","volume":"63","author":"K Athenstaedt","year":"2006","unstructured":"Athenstaedt, K. & Daum, G. The life cycle of neutral lipids: synthesis, storage and degradation. Cell Mol. Life Sci. 63, 1355\u20131369 (2006).","journal-title":"Cell Mol. Life Sci."},{"key":"BFja2014100_CR2","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1007\/978-1-4615-5901-6_9","volume":"28","author":"WJ Johnson","year":"1997","unstructured":"Johnson, W. J., Phillips, M. C. & Rothblat, G. H. Lipoproteins and cellular cholesterol homeostasis. Subcell. Biochem. 28, 235\u2013276 (1997).","journal-title":"Subcell. Biochem."},{"key":"BFja2014100_CR3","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1073\/pnas.76.1.333","volume":"76","author":"JL Goldstein","year":"1979","unstructured":"Goldstein, J. L., Ho, Y. K., Basu, S. K. & Brown, M. S. Binding site on macrophages that mediates uptake and degradation of acetylated low density lipoprotein, producing massive cholesterol deposition. Proc. Natl. Acad. Sci. USA 76, 333\u2013337 (1979).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFja2014100_CR4","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1083\/jcb.82.3.597","volume":"82","author":"MS Brown","year":"1979","unstructured":"Brown, M. S., Goldstein, J. L., Krieger, M., Ho, Y. K. & Anderson, R. G. Reversible accumulation of cholesteryl esters in macrophages incubated with acetylated lipoproteins. J. Cell Biol. 82, 597\u2013613 (1979).","journal-title":"J. Cell Biol."},{"key":"BFja2014100_CR5","first-page":"57","volume":"100","author":"T Schaffner","year":"1980","unstructured":"Schaffner, T. et al. Arterial foam cells with distinctive immunomorphologic and histochemical features of macrophages. Am. J. Pathol. 100, 57\u201373 (1980).","journal-title":"Am. J. Pathol."},{"key":"BFja2014100_CR6","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1016\/j.cell.2011.04.005","volume":"145","author":"KJ Moore","year":"2011","unstructured":"Moore, K. J. & Tabas, I. Machophages in the pathogenesis of atherosclerosis. Cell 145, 341\u2013355 (2011).","journal-title":"Cell"},{"key":"BFja2014100_CR7","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1038\/ja.2012.12","volume":"65","author":"T Ohshiro","year":"2012","unstructured":"Ohshiro, T. et al. New verticilides, inhibitors of acyl-CoA:cholesterol acyltransferase, produced by Verticillium sp. FKI-2679. J. Antibiot. 65, 255\u2013262 (2012).","journal-title":"J. Antibiot."},{"key":"BFja2014100_CR8","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1038\/ja.2011.32","volume":"64","author":"S Ohte","year":"2011","unstructured":"Ohte, S. et al. Dinapinones, novel inhibitor of triacylglycerol synthesis in mammalian cells, produced by Penicillium pinophilum FKI-3864. J. Antibiot. 64, 489\u2013494 (2011).","journal-title":"J. Antibiot."},{"key":"BFja2014100_CR9","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1038\/ja.2011.105","volume":"65","author":"N Ugaki","year":"2012","unstructured":"Ugaki, N. et al. New isochaetochromin, an inhibitor of triacylglycerol synthesis in mammalian cells, produced by Penicillium sp. FKI-4942: I. Taxonomy, fermentation, isolation and biological properties. J. Antibiot. 65, 15\u201319 (2012).","journal-title":"J. Antibiot."},{"key":"BFja2014100_CR10","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","volume":"215","author":"SF Altschul","year":"1990","unstructured":"Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. Basic local alignment search tool. J. Mol. Biol. 215, 403\u2013410 (1990).","journal-title":"J. Mol. Biol."},{"key":"BFja2014100_CR11","doi-asserted-by":"publisher","first-page":"110","DOI":"10.7164\/antibiotics.37.110","volume":"37","author":"G Warner","year":"1984","unstructured":"Warner, G. et al. Metabolic products of macroorganisms. 224. Bafilomycins, a new group of macrolide antibiotics. Production, isolation, chemical structure and biological activity. J. Antibiot. 37, 110\u2013117 (1984).","journal-title":"J. Antibiot."},{"key":"BFja2014100_CR12","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1038\/ja.2010.52","volume":"63","author":"S Tchize Ndejouong Ble.","year":"2010","unstructured":"Tchize Ndejouong Ble., S. et al. Hygrobafilomycin, a cytotoxic and antifungal macrolide bearing a unique monoalkylmaleic anhydride moiety, from Streptomyces varsoviensis. J. Antibiot. 63, 359\u2013363 (2010).","journal-title":"J. Antibiot."},{"key":"BFja2014100_CR13","doi-asserted-by":"publisher","first-page":"1073","DOI":"10.7164\/antibiotics.50.1073","volume":"50","author":"MG O\u2019Shea","year":"1997","unstructured":"O\u2019Shea, M. G., Rickards, R. W., Rothschild, J. M. & Lacey, E. Absolute configurations of macrolide antibiotics of the bafilomycin and leucanicidin groups. J. Antibiot. 50, 1073\u20131077 (1997).","journal-title":"J. Antibiot."},{"key":"BFja2014100_CR14","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1093\/oxfordjournals.jbchem.a022289","volume":"125","author":"I Namatame","year":"1999","unstructured":"Namatame, I., Tomoda, H., Arai, H., Inoue, K. & \u014cmura, S. Complete inhibition of mouse macrophage-derived foam cell formation by triacsin C. J. Biochem. 125, 319\u2013327 (1999).","journal-title":"J. Biochem."},{"key":"BFja2014100_CR15","doi-asserted-by":"publisher","first-page":"7972","DOI":"10.1073\/pnas.85.21.7972","volume":"85","author":"EJ Bowman","year":"1988","unstructured":"Bowman, E. J., Siebers, A. & Altendorf, K. Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells. Proc. Natl. Acad. Sci. USA 85, 7972\u20137976 (1988).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFja2014100_CR16","doi-asserted-by":"publisher","first-page":"3902","DOI":"10.1021\/bi00066a008","volume":"32","author":"S Drose","year":"1993","unstructured":"Drose, S. et al. Inhibitory effect of modified bafilomycins and concanamycins on P- and V-type adenosinetriphosphatases. Biochemistry 32, 3902\u20133906 (1993).","journal-title":"Biochemistry"},{"key":"BFja2014100_CR17","doi-asserted-by":"publisher","first-page":"40481","DOI":"10.1074\/jbc.M509106200","volume":"280","author":"YR Wang","year":"2005","unstructured":"Wang, Y. R., Inoue, T. & Forgac, M. Subunit a of the yeast V-ATPase participates in binding of bafilomycin. J. Biol. Chem. 280, 40481\u201340488 (2005).","journal-title":"J. Biol. Chem."},{"key":"BFja2014100_CR18","doi-asserted-by":"publisher","first-page":"1364","DOI":"10.1271\/bbb.50644","volume":"70","author":"S Kazami","year":"2006","unstructured":"Kazami, S. et al. Iejimalides show anti-osteoclast activity via V-ATPase inhibition. Biosci. Biotechnol. Biochem. 70, 1364\u20131370 (2006).","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"BFja2014100_CR19","doi-asserted-by":"publisher","first-page":"33131","DOI":"10.1074\/jbc.M404638200","volume":"279","author":"EJ Bowman","year":"2004","unstructured":"Bowman, E. J., Graham, L. A., Stevens, T. H. & Bowman, B. J. The bafilomycin\/concanamycin binding site in subunit c of the V-ATPases from Neurospora crassa and Saccharomyces cerevisiae. J. Biol. Chem. 279, 33131\u201333138 (2004).","journal-title":"J. Biol. Chem."},{"key":"BFja2014100_CR20","first-page":"2509","volume":"49","author":"A Kretschmer","year":"1985","unstructured":"Kretschmer, A., Drogerloh, M., Deeg, M. & Hagenmaier, H. The structures of novel insecticidal macrolides bafilomycins D and E, and oxohygrolidin. Agric. Biol. Chem. 49, 2509\u20132511 (1985).","journal-title":"Agric. Biol. Chem."},{"key":"BFja2014100_CR21","doi-asserted-by":"publisher","first-page":"422","DOI":"10.1021\/np900632r","volume":"73","author":"G Carr","year":"2010","unstructured":"Carr, G. et al. Bafilomycins produced in culture by Streptomyces spp. isolated from marine habitats are potent inhibitors autophagy. J. Nat. Prod. 73, 422\u2013427 (2010).","journal-title":"J. Nat. Prod."},{"key":"BFja2014100_CR22","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1038\/ja.2011.12","volume":"64","author":"DJ Zhang","year":"2011","unstructured":"Zhang, D. J. et al. Bafilomycin K, a new antifungal macrolide from Streptomyces flavotricini Y12-26. J. Antibiot. 64, 391\u2013393 (2011).","journal-title":"J. Antibiot."},{"key":"BFja2014100_CR23","doi-asserted-by":"publisher","first-page":"610","DOI":"10.7164\/antibiotics.37.610","volume":"37","author":"H Seto","year":"1984","unstructured":"Seto, H., Tajima, I., Akao, H., Furihata, K. & Otake, N. The isolation and structures of hygrolidin amide and defumarylhygrolidin. J. Antibiot. 37, 610\u2013613 (1984).","journal-title":"J. Antibiot."},{"key":"BFja2014100_CR24","doi-asserted-by":"publisher","first-page":"1253","DOI":"10.7164\/antibiotics.34.1253","volume":"34","author":"S Omura","year":"1981","unstructured":"Omura, S., Otoguro, K., Nishikiori, T., Oiwa, R. & Iwai, Y. Setamycin, a new antibiotic. J. Antibiot. 34, 1253\u20131256 (1981).","journal-title":"J. Antibiot."},{"key":"BFja2014100_CR25","doi-asserted-by":"publisher","first-page":"250","DOI":"10.7164\/antibiotics.41.250","volume":"41","author":"K Otoguro","year":"1988","unstructured":"Otoguro, K., Nakagawa, A. & Omura, S. Setamycin, a 16-membered macrolide antibiotic. Identification and nematocidal activity. J. Antibiot. 41, 250\u2013252 (1988).","journal-title":"J. Antibiot."},{"key":"BFja2014100_CR26","doi-asserted-by":"publisher","first-page":"627","DOI":"10.1038\/ja.2010.104","volume":"63","author":"J Ueda","year":"2010","unstructured":"Ueda, J. et al. A new 16-membered tetraene macrolide JBIR-100 from a newly identified Streptomyces species. J. Antibiot. 63, 627\u2013629 (2010).","journal-title":"J. Antibiot."},{"key":"BFja2014100_CR27","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1038\/ja.2012.24","volume":"65","author":"S Kodani","year":"2012","unstructured":"Kodani, S., Murao, A., Hidaki, M., Sato, K. & Ogawa, N. Isolation and structural determination of a new macrolide, makinolide, from the newly isolated Streptomyces sp. MK-30. J. Antibiot. 65, 331\u2013334 (2012).","journal-title":"J. Antibiot."},{"key":"BFja2014100_CR28","doi-asserted-by":"publisher","first-page":"1964","DOI":"10.1271\/bbb.130204","volume":"77","author":"M Hidaki","year":"2013","unstructured":"Hidaki, M., Yoshida, M., Ogawa, N. & Kodani, S. Isolation and structural determination of makinolide B from Streptomyces sp. MK-19. Biosci. Biotechnol. Biochem. 77, 1964\u20131966 (2013).","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"BFja2014100_CR29","doi-asserted-by":"publisher","first-page":"787","DOI":"10.2174\/0929867033457827","volume":"10","author":"JA Beutler","year":"2003","unstructured":"Beutler, J. A. & McKee, T. C. Novel marine and microbial natural product inhibitors of vascuolar ATPase. Curr. Med. Chem. 10, 787\u2013796 (2003).","journal-title":"Curr. Med. Chem."},{"key":"BFja2014100_CR30","first-page":"443","volume":"22","author":"J Xu","year":"2007","unstructured":"Xu, J., Cheng, T., Feng, H. T., Pavlos, N. J. & Zheng, M. H. Structure and function of V-ATPases in osteoclasts: potential therapeutic targets for the treatment of osteolysis. Histol. Histopathol. 22, 443\u2013454 (2007).","journal-title":"Histol. Histopathol."},{"key":"BFja2014100_CR31","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1111\/j.1471-4159.2010.06838.x","volume":"114","author":"VN Pivtoraiko","year":"2010","unstructured":"Pivtoraiko, V. N. et al. Low-dose bafilomycin attenuates neuronal cell death associated with autophagy-lysosome pathway dysfunction. J. Neurochem. 114, 1193\u20131204 (2010).","journal-title":"J. Neurochem."},{"key":"BFja2014100_CR32","doi-asserted-by":"crossref","first-page":"27345","DOI":"10.1016\/S0021-9258(19)74255-0","volume":"268","author":"T Furuchi","year":"1993","unstructured":"Furuchi, T., Aikawa, K., Arai, H. & Inoue, K. Bafilomycin A1, a specific inhibitor of vacuolar-type H(+)-ATPase, blocks lysosomal cholesterol trafficking in macrophages. J. Biol. Chem. 268, 27345\u201327348 (1993).","journal-title":"J. Biol. Chem."},{"key":"BFja2014100_CR33","doi-asserted-by":"publisher","first-page":"1333","DOI":"10.7164\/antibiotics.37.1333","volume":"37","author":"H Kinashi","year":"1984","unstructured":"Kinashi, H., Someno, K. & Sakaguchi, K. Isolation and characterization of concanamycins A, B, and C. J. Antibiot. 37, 1333\u20131343 (1984).","journal-title":"J. Antibiot."},{"key":"BFja2014100_CR34","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/0005-2760(92)90214-G","volume":"1126","author":"S Naganuma","year":"1992","unstructured":"Naganuma, S., Kuzuya, N., Sakai, K., Hasumi, K. & Endo, A. Inhibition of the accumulation of lipid droplets in macrophage J774 by bafilomycin B1 and destruxin E. Biochim. Biophys. Acta 1126, 41\u201348 (1992).","journal-title":"Biochim. Biophys. Acta"},{"key":"BFja2014100_CR35","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1111\/j.1432-1033.1992.tb17061.x","volume":"207","author":"JT Woo","year":"1992","unstructured":"Woo, J. T., Shinohara, C., Sakai, K., Hasumi, K. & Endo, A. Inhibition of the acidification of endosomes and lysosomes by the antibiotic concanamycin B in macrophage J774. Eur. J. Biochem. 207, 383\u2013389 (1992).","journal-title":"Eur. J. Biochem."},{"key":"BFja2014100_CR36","first-page":"19","volume":"26","author":"A Eshima","year":"2007","unstructured":"Eshima, A., Wada, T., Tsubine, T. & Ogata, T. An efficient rice variety discrimination system using simple sequence repeat markers. Bull. Fukuoka Agric. Res. Cent. 26, 19\u201323 (2007).","journal-title":"Bull. Fukuoka Agric. Res. Cent."},{"key":"BFja2014100_CR37","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1194\/jlr.D300037-JLR200","volume":"45","author":"A Lada","year":"2004","unstructured":"Lada, A. et al. Identification of ACAT1- and ACAT2-specific inhibitors using a novel, cell-based fluorescence assay: individual ACAT uniqueness. J. Lipid Res. 45, 378\u2013386 (2004).","journal-title":"J. Lipid Res."},{"key":"BFja2014100_CR38","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1038\/ja.2007.6","volume":"60","author":"T Ohshiro","year":"2007","unstructured":"Ohshiro, T., Rudel, L. L., \u014cmura, S. & Tomoda, H. Selectivity of microbial acyl-CoA: cholesterol acyltransferase inhibitors toward isozymes. J. Antibiot. 60, 43\u201351 (2007).","journal-title":"J. Antibiot."},{"key":"BFja2014100_CR39","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1139\/y59-099","volume":"37","author":"EG Bligh","year":"1959","unstructured":"Bligh, E. G. & Dyer, W. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911\u2013917 (1959).","journal-title":"Can. J. Biochem. Physiol."},{"key":"BFja2014100_CR40","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1016\/S0021-9258(20)71211-1","volume":"260","author":"E Uchida","year":"1985","unstructured":"Uchida, E., Ohsumi, Y. & Anraku, Y. Purification and properties of H+-translocationg, Mg2+-adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. J. Biol. Chem. 260, 1090\u20131095 (1985).","journal-title":"J. Biol. Chem."}],"container-title":["The Journal of Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/ja2014100.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/ja2014100","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/ja2014100.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T23:56:57Z","timestamp":1717199817000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/ja2014100"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,6]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["BFja2014100"],"URL":"https:\/\/doi.org\/10.1038\/ja.2014.100","relation":{},"ISSN":["0021-8820","1881-1469"],"issn-type":[{"value":"0021-8820","type":"print"},{"value":"1881-1469","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,6]]},"assertion":[{"value":"22 April 2014","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 May 2014","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 May 2014","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 August 2014","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}