{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:36:00Z","timestamp":1773282960650,"version":"3.50.1"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Antibiot"],"published-print":{"date-parts":[[2015,4]]},"DOI":"10.1038\/ja.2014.133","type":"journal-article","created":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T06:48:56Z","timestamp":1412146136000},"page":"271-278","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Discovery of a capuramycin analog that kills nonreplicating Mycobacterium tuberculosis and its synergistic effects with translocase I inhibitors"],"prefix":"10.1038","volume":"68","author":[{"given":"Shajila","family":"Siricilla","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuhiko","family":"Mitachi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bajoie","family":"Wan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott G","family":"Franzblau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michio","family":"Kurosu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2014,10,1]]},"reference":[{"key":"BFja2014133_CR1","first-page":"1621","volume":"14","author":"D Nakanjako","year":"2010","unstructured":"Nakanjako D et al. Tuberculosis and human immunodeficiency virus co-infections and their predictors at a hospital-based HIV\/AIDS clinic in Uganda. Int. J. Tuberc. Lung Dis. 14, 1621\u20131628 (2010).","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"BFja2014133_CR2","doi-asserted-by":"publisher","first-page":"1407","DOI":"10.1128\/IAI.01126-10","volume":"79","author":"CR Diedrich","year":"2011","unstructured":"Diedrich CR, Flynn JL HIV-1\/Mycobacterium tuberculosis co-infection immunology: How does HIV-1 exacerbate tuberculosis? Infect. Immun. 79, 1407\u20131417 (2011).","journal-title":"Infect. Immun."},{"key":"BFja2014133_CR3","doi-asserted-by":"publisher","first-page":"1910","DOI":"10.1093\/jac\/dkt103","volume":"68","author":"JY Chien","year":"2013","unstructured":"Chien JY et al. Decline in rates of acquired multidrug-resistant tuberculosis after implementation of the directly observed therapy, short course (DOTS) and DOTS-Plus programmes in Taiwan. J. Antimicrob. Chemother. 68, 1910\u20131916 (2013).","journal-title":"J. Antimicrob. Chemother."},{"key":"BFja2014133_CR4","doi-asserted-by":"publisher","first-page":"e6116","DOI":"10.1371\/journal.pone.0006116","volume":"4","author":"S Ranjbar","year":"2009","unstructured":"Ranjbar S et al. HIV-1 Replication is differentially regulated by distinct clinical strains of Mycobacterium tuberculosis. PLoS ONE 4, e6116 (2009).","journal-title":"PLoS ONE"},{"key":"BFja2014133_CR5","first-page":"435","volume":"4","author":"LE Connolly","year":"2003","unstructured":"Connolly LE, Edelstein PH, Ramakrishnan L Why is long-term therapy required to cure tuberculosis? PLoS Med. 4, 435\u2013442 (2003).","journal-title":"PLoS Med."},{"key":"BFja2014133_CR6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2012\/139127","volume":"2012","author":"MS Miranda","year":"2012","unstructured":"Miranda MS, Breiman A, Allain S, Deknuydt F, Altare F The tuberculous granuloma: an unsuccessful host defense mechanism providing a safety shelter for the bacteria? Clin. Develop. Immun. 2012, 1\u201314 (2012).","journal-title":"Clin. Develop. Immun."},{"key":"BFja2014133_CR7","doi-asserted-by":"crossref","first-page":"2062","DOI":"10.1128\/IAI.64.6.2062-2069.1996","volume":"64","author":"LG Wayne","year":"1996","unstructured":"Wayne LG, Hayes LG An in vitro model for sequential study of shiftdown of Mycobacterium tuberculosis through two stages of nonreplicating persistence. Infect. Immun. 64, 2062\u20132069 (1996).","journal-title":"Infect. Immun."},{"key":"BFja2014133_CR8","doi-asserted-by":"publisher","first-page":"4150","DOI":"10.1128\/AAC.00821-10","volume":"54","author":"S Claudia","year":"2010","unstructured":"Claudia S et al. Simple model for testing drugs against nonreplicating Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 54, 4150\u20134158 (2010).","journal-title":"Antimicrob. Agents Chemother."},{"key":"BFja2014133_CR9","doi-asserted-by":"publisher","first-page":"620","DOI":"10.1128\/MMBR.00016-07","volume":"71","author":"J van Heijenoort","year":"2007","unstructured":"van Heijenoort J Lipid intermediates in the biosynthesis of bacterial peptidoglycan. Microbiol. Mol. Biol. Rev. 71, 620\u2013635 (2007).","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"BFja2014133_CR10","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1128\/MMBR.00028-07","volume":"72","author":"EC Hett","year":"2008","unstructured":"Hett EC, Rubin EJ Bacterial growth and cell division: a Mycobacterial perspective. Microbiol. Mol. Biol. Rev. 72, 126\u2013156 (2008).","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"BFja2014133_CR11","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1016\/j.cbpa.2010.04.008","volume":"14","author":"CE Barry","year":"2010","unstructured":"Barry CE, Blanchard JS The chemical biology of new drugs in development for tuberculosis. Curr. Opin. Chem. Biol. 14, 456\u2013466 (2010).","journal-title":"Curr. Opin. Chem. Biol."},{"key":"BFja2014133_CR12","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1128\/AAC.01469-07","volume":"52","author":"VM Reddy","year":"2008","unstructured":"Reddy VM, Einck L, Nacy CA In vitro antimycobacterial activity of capuramycin analogs. Antimicro. Agents Chemother. 52, 719\u2013721 (2008).","journal-title":"Antimicro. Agents Chemother."},{"key":"BFja2014133_CR13","doi-asserted-by":"publisher","first-page":"217","DOI":"10.3987\/COM-08-S(F)38","volume":"77","author":"M Kurosu","year":"2009","unstructured":"Kurosu M, Li K Synthetic studies towards the identification of novel capuramycin analogs with antimycobacterial activity. Heterocycles 77, 217\u2013225 (2009).","journal-title":"Heterocycles"},{"key":"BFja2014133_CR14","doi-asserted-by":"publisher","first-page":"2393","DOI":"10.1021\/ol900458w","volume":"11","author":"M Kurosu","year":"2009","unstructured":"Kurosu M, Li K, Crick DC A concise synthesis of capuramycin. Org. Lett. 11, 2393\u20132396 (2009).","journal-title":"Org. Lett."},{"key":"BFja2014133_CR15","doi-asserted-by":"publisher","first-page":"13847","DOI":"10.1002\/chem.201302389","volume":"19","author":"Y Wang","year":"2013","unstructured":"Wang Y, Siricilla S, Aleiwi BA, Kurosu M Improved synthesis of capuramycin and its analogues. Chem. Eur. J. 19, 13847\u201313858 (2013).","journal-title":"Chem. Eur. J."},{"key":"BFja2014133_CR16","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1093\/jac\/dkh417","volume":"54","author":"T Koga","year":"2004","unstructured":"Koga T et al. Activity of capuramycin analogs against Mycobacterium tuberculosis, Mycobacterium avium and Mycobacterium intracellular in vitro and in vivo. J. Antimicro. Chemother. 54, 755\u2013760 (2004).","journal-title":"J. Antimicro. Chemother."},{"key":"BFja2014133_CR17","doi-asserted-by":"publisher","first-page":"3138","DOI":"10.1128\/AAC.00366-09","volume":"53","author":"BV Nikonenko","year":"2009","unstructured":"Nikonenko BV et al. Activity of SQ641, a capuramycin analog, in a murine model of tuberculosis. Antimicrob. Agents Chemother. 53, 3138\u20133139 (2009).","journal-title":"Antimicrob. Agents Chemother."},{"key":"BFja2014133_CR18","doi-asserted-by":"publisher","first-page":"2590","DOI":"10.1093\/jac\/dkq372","volume":"65","author":"T Dubuisson","year":"2010","unstructured":"Dubuisson T et al. In vitro antimicrobial activities of capuramycin analogues against non-tuberculous mycobacteria. J. Antimicrob. Chemother. 65, 2590\u20132597 (2010).","journal-title":"J. Antimicrob. Chemother."},{"key":"BFja2014133_CR19","doi-asserted-by":"publisher","first-page":"578","DOI":"10.1093\/jac\/dkq495","volume":"66","author":"E Bogatcheva","year":"2011","unstructured":"Bogatcheva E et al. Chemical modification of capuramycins to enhance antibacterial activity. J. Antimicrob. Chemother. 66, 578\u2013587 (2011).","journal-title":"J. Antimicrob. Chemother."},{"key":"BFja2014133_CR20","doi-asserted-by":"publisher","first-page":"1380","DOI":"10.1128\/AAC.00055-06","volume":"51","author":"SH Cho","year":"2007","unstructured":"Cho SH et al. Low-oxygen-recovery assay for high-throughput screening of compounds against nonreplicating Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 51, 1380\u20131358 (2007).","journal-title":"Antimicrob. Agents Chemother."},{"key":"BFja2014133_CR21","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.ab.2014.05.018","volume":"461","author":"S Siricilla","year":"2014","unstructured":"Siricilla S, Mitachi K, Skorupinska-Tudek K, Swiezewska E, Kurosu M Biosynthesis of a water-soluble lipid I analogue and a convenient assay for translocase I. Anal. Biochem. 461, 36\u201335 (2014).","journal-title":"Anal. Biochem."},{"key":"BFja2014133_CR22","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1128\/IAI.24.2.363-370.1979","volume":"24","author":"LG Wayne","year":"1979","unstructured":"Wayne LG, Sramek HA Antigenic differences between extracts of actively replicating and synchronized resting cells of Mycobacterium tuberculosis. Infect. Immun. 24, 363\u2013370 (1979).","journal-title":"Infect. Immun."},{"key":"BFja2014133_CR23","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1099\/mic.0.033084-0","volume":"156","author":"M Gengenbacher","year":"2010","unstructured":"Gengenbacher M, Rao SPS, Pethe K, Dick T Nutrient-starved, non-replicating Mycobacterium tuberculosis requires respiration, ATP synthase and isocitrate lyase for maintenance of ATP homeostasis and viability. Microbiology 156, 81\u201387 (2010).","journal-title":"Microbiology"},{"key":"BFja2014133_CR24","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1016\/0732-8893(93)90087-N","volume":"16","author":"MH Hsieh","year":"1993","unstructured":"Hsieh MH, Chen MY, Victor LY, Chow JW Synergy assessed by checkerboard. A critical analysis. Diagn. Microbiol. Infect. Dis. 16, 343\u2013349 (1993).","journal-title":"Microbiol. Infect. Dis."},{"key":"BFja2014133_CR25","doi-asserted-by":"publisher","first-page":"602","DOI":"10.1128\/AAC.00999-09","volume":"54","author":"J Meletiadis","year":"2010","unstructured":"Meletiadis J, Pournaras S, Roilides E, Walsh TJ Defining fractional inhibitory concentration index cutoffs for additive interactions based on self-drug additive combinations, monte carlo simulation analysis, and in vitro-in vivo correlation data for antifungal drug combinations against Aspergillus fumigatus. Antimicrob. Agents Chemother. 54, 602\u2013609 (2010).","journal-title":"Antimicrob. Agents Chemother."},{"key":"BFja2014133_CR26","doi-asserted-by":"publisher","first-page":"3739","DOI":"10.1021\/jm201608g","volume":"55","author":"J Debnath","year":"2012","unstructured":"Debnath J et al. Discovery of selective menaquinone biosynthesis inhibitors against Mycobacterium tuberculosis. J. Med. Chem. 55, 3739\u20133755 (2012).","journal-title":"J. Med. Chem."},{"key":"BFja2014133_CR27","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1111\/j.1365-2958.1993.tb01572.x","volume":"8","author":"ME Levin","year":"1993","unstructured":"Levin ME, Hatfull GF Mycobacterium smegmatis RNA polymerase: DNA supercoiling, action of rifampicin and mechanism of rifampicin resistance. Mol. Microbiol. 8, 277\u2013285 (1993).","journal-title":"Mol. Microbiol."},{"key":"BFja2014133_CR28","doi-asserted-by":"publisher","first-page":"9870","DOI":"10.1039\/c2cc35028a","volume":"48","author":"V Siegmund","year":"2012","unstructured":"Siegmund V, Santner T, Micura R, Marx A Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotides. Chem. Commun. 48, 9870\u20139872 (2012).","journal-title":"Chem. Commun."},{"key":"BFja2014133_CR29","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1016\/j.ab.2003.08.038","volume":"324","author":"P Kuhlman","year":"2004","unstructured":"Kuhlman P, Duff HL, Galant A A fluorescence-based assay for multisubunit DNA-dependent RNA polymerases. Analy. Biochem. 324, 183\u2013190 (2004).","journal-title":"Analy. Biochem."},{"key":"BFja2014133_CR30","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1273\/cbij.11.52","volume":"11","author":"K Onodera","year":"2011","unstructured":"Onodera K, Kawasaki T, Kamijo S Discovery of novel antimicrobial agents targeting the bacterial RNA polymerase by high-throughput virtual screening. Chem-Bio. Informat. J. 11, 52\u201362 (2011).","journal-title":"Chem-Bio. Informat. J."},{"key":"BFja2014133_CR31","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1038\/nrmicro1065","volume":"3","author":"HIM Boshoff","year":"2005","unstructured":"Boshoff HIM, Barry CE Tuberculosis - metabolism and respiration in the absence of growth. Nature Rev. Microbiol. 3, 70\u201380 (2005).","journal-title":"Nature Rev. Microbiol"},{"key":"BFja2014133_CR32","doi-asserted-by":"publisher","first-page":"30309","DOI":"10.1074\/jbc.M113.492173","volume":"288","author":"Y Ishizaki","year":"2013","unstructured":"Ishizaki Y et al. Inhibition of the first step in synthesis of the Mycobacterial cell wall core, catalyzed by the GlcNAc-1-phosphate transferase WecA, by the novel caprazamycin derivative CPZEN-45. J. Biol. Chem. 288, 30309\u201330319 (2013).","journal-title":"J. Biol. Chem."},{"key":"BFja2014133_CR33","doi-asserted-by":"publisher","first-page":"1789","DOI":"10.1517\/13543784.2012.724397","volume":"21","author":"J Engohang-Ndong","year":"2012","unstructured":"Engohang-Ndong J Antimycobacterial drugs currently in phase II clinical trials and preclinical phase for tuberculosis treatment. Expert Opin. Investig. Drugs. 21, 1789\u20131800 (2012).","journal-title":"Expert Opin. Investig. Drugs."},{"key":"BFja2014133_CR34","doi-asserted-by":"publisher","first-page":"901","DOI":"10.1016\/S0092-8674(01)00286-0","volume":"104","author":"EA Campbell","year":"2001","unstructured":"Campbell EA et al. Structural mechanism for rifampicin inhibition of bacterial RNA polymerase. Cell 104, 901\u2013912 (2001).","journal-title":"Cell"},{"key":"BFja2014133_CR35","doi-asserted-by":"crossref","first-page":"4586","DOI":"10.1128\/IAI.67.9.4586-4593.1999","volume":"67","author":"VA Snewin","year":"1999","unstructured":"Snewin VA et al. Assessment of immunity to mycobacterial infection with luciferase reporter constructs. Infect. Immun. 67, 4586\u20134593 (1999).","journal-title":"Infect. Immun."},{"key":"BFja2014133_CR36","doi-asserted-by":"publisher","first-page":"799","DOI":"10.1016\/j.tetlet.2006.11.160","volume":"48","author":"M Kurosu","year":"2007","unstructured":"Kurosu M, Mahapatra S, Narayanasamy P, Crick DC Chemoenzymatic synthesis of park\u2019s nucleotide: toward the development of high-throughput screening for MraY inhibitors. Tetrahedron Lett. 48, 799\u2013803 (2007).","journal-title":"Tetrahedron Lett."},{"key":"BFja2014133_CR37","doi-asserted-by":"publisher","first-page":"455","DOI":"10.3987\/COM-08-S(N)30","volume":"76","author":"K Li","year":"2008","unstructured":"Li K, Kurosu M Synthetic studies on Mycobacterium tuberculosis specific fluorescent park\u2019s nucleotide probe. Heterocycles 76, 455\u2013469 (2008).","journal-title":"Heterocycles"},{"key":"BFja2014133_CR38","doi-asserted-by":"publisher","first-page":"4554","DOI":"10.1002\/chem.201400307","volume":"20","author":"K Mitachi","year":"2014","unstructured":"Mitachi K, Mohan P, Siricilla S, Kurosu M One-pot protection-glycosylation reactions for synthesis of lipid II analogues. Chem. A. Eur. J. 20, 4554\u20134558 (2014).","journal-title":"Chem. A. Eur. J."},{"key":"BFja2014133_CR39","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.mimet.2006.05.016","volume":"68","author":"R Mandana","year":"2007","unstructured":"Mandana R, Marie-Antoinette L, Peter S, Mamadou D Breaking down the wall: Fractionation of mycobacteria. J. Microbiol. Methods 68, 32\u201339 (2007).","journal-title":"J. Microbiol. Methods"},{"key":"BFja2014133_CR40","doi-asserted-by":"publisher","first-page":"29974","DOI":"10.1074\/jbc.M314165200","volume":"279","author":"A Bouhss","year":"2004","unstructured":"Bouhss A, Crouvoisier M, Blanot D, Mengin-Lecreulx D Purification and characterization of the bacterial MraY translocase catalyzing the first membrane step of peptidoglycan biosynthesis. J. Biol. Chem. 279, 29974\u201329980 (2004).","journal-title":"J. Biol. Chem."},{"key":"BFja2014133_CR41","doi-asserted-by":"publisher","first-page":"4634","DOI":"10.1021\/bi00692a011","volume":"14","author":"RR Burgess","year":"1975","unstructured":"Burgess RR, Jendrisak JJ A procedure for the rapid, large-scale purification of Escherichia coli DNA-dependent RNA polymerase involving polymin P precipitation and DNA-cellulose chromatography. Biochemistry 14, 4634\u20134638 (1975).","journal-title":"Biochemistry"},{"key":"BFja2014133_CR42","doi-asserted-by":"publisher","first-page":"2518","DOI":"10.1128\/AAC.46.8.2518-2524.2002","volume":"46","author":"C Ohrt","year":"2002","unstructured":"Ohrt C, Willingmyre GD, Lee P, Knirsch C, Milhous W Assessment of azithromycin in combination with other antimalarial drugs against Plasmodium falciparum in vitro. Antimicrob. Agents Chemother. 46, 2518\u20132524 (2002).","journal-title":"Antimicrob. Agents Chemother."}],"container-title":["The Journal of Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/ja2014133.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/ja2014133","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/ja2014133.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T17:20:41Z","timestamp":1684430441000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/ja2014133"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,10,1]]},"references-count":42,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["BFja2014133"],"URL":"https:\/\/doi.org\/10.1038\/ja.2014.133","relation":{},"ISSN":["0021-8820","1881-1469"],"issn-type":[{"value":"0021-8820","type":"print"},{"value":"1881-1469","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,10,1]]},"assertion":[{"value":"24 June 2014","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 September 2014","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 September 2014","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 October 2014","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}