{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T07:08:45Z","timestamp":1777792125853,"version":"3.51.4"},"reference-count":24,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[1993,9,1]],"date-time":"1993-09-01T00:00:00Z","timestamp":746841600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cereb Blood Flow Metab"],"published-print":{"date-parts":[[1993,9]]},"abstract":"<jats:p>\n                    During the first weeks of life, injury to the central nervous system caused by brief periods of oxygen deprivation greatly increases. To investigate possible causes for this change, the effects of hypoxia or application of the excitatory neurotransmitter glutamate on intracellular calcium ([Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    ) and ATP were studied in rat cerebrocortical brain slices. [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    was measured fluo-rometrically with the indicator Fura-2. Hypoxia (95% N\n                    <jats:sub>2<\/jats:sub>\n                    \/ 5% CO\n                    <jats:sub>2<\/jats:sub>\n                    ) or 100 \u03bc M sodium cyanide produced gradual elevations in [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    and ATP depletion in slices from rats &lt;2 weeks old, but rapid changes in older rats. After 20 min, [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    in adult slices exposed to cyanide was 1,980 \u00b1 310 n M; in day 1\u201314 animals, it was 796 \u00b1 181 n M (p &lt; 0.05). Combination of cyanide and a glycolytic inhibitor (iodoacetate) rapidly elevated [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    and depleted ATP in all age groups. Energy utilization during anoxia, assessed by measuring ATP fall in cyanide\/ iodoacetate-treated brain slices, increased with age. Elevations in [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    caused by application of 500 \u03bc M glutamate increased 240% from days 1\u20132 to day 28, but ATP loss caused by glutamate did not change with age. The N-methyl-d-aspartate antagonist MK-801 delayed calcium entry during the initial 5\u20137 min of hypoxia or cyanide in rats &lt;2 weeks old. We conclude that anaerobic ATP production, conservation of energy by reduced ATP consumption, and reduced sensitivity to glutamate contribute to delaying elevation in [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    in neonatal rat brain during hypoxia.\n                  <\/jats:p>","DOI":"10.1038\/jcbfm.1993.103","type":"journal-article","created":{"date-parts":[[2011,4,8]],"date-time":"2011-04-08T13:13:04Z","timestamp":1302268384000},"page":"811-819","source":"Crossref","is-referenced-by-count":66,"title":["Developmental Changes in Intracellular Calcium Regulation in Rat Cerebral Cortex during Hypoxia"],"prefix":"10.1177","volume":"13","author":[{"given":"Philip E.","family":"Bickler","sequence":"first","affiliation":[{"name":"Department of Anesthesia, University of California, San Francisco, California, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven M.","family":"Gallego","sequence":"additional","affiliation":[{"name":"Department of Anesthesia, University of California, San Francisco, California, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bonnie M.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Anesthesia, University of California, San Francisco, California, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1993,9,1]]},"reference":[{"key":"bibr1-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1948.155.3.366"},{"key":"bibr2-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.10-03-00909.1990"},{"key":"bibr3-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(90)90199-S"},{"key":"bibr4-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(88)90200-7"},{"key":"bibr5-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1975.tb11875.x"},{"key":"bibr6-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1941.134.2.281"},{"key":"bibr7-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp018265"},{"key":"bibr8-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1977.tb10394.x"},{"key":"bibr9-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1985.65.1.101"},{"key":"bibr10-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1038\/357686a0"},{"key":"bibr11-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.11-06-01674.1991"},{"key":"bibr12-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1989.sp017638"},{"key":"bibr13-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(76)90277-6"},{"key":"bibr14-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(88)90306-X"},{"key":"bibr15-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1038\/307462a0"},{"key":"bibr16-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410190202"},{"key":"bibr17-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(89)90017-4"},{"key":"bibr18-jcbfm.1993.103","first-page":"223","volume":"8","author":"Siesj\u00f6 BK","year":"1989","journal-title":"Magnesium"},{"key":"bibr19-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1989.20"},{"key":"bibr20-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1988.140"},{"key":"bibr21-jcbfm.1993.103","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1969.216.2.348"},{"key":"bibr22-jcbfm.1993.103","first-page":"357","volume-title":"Methods of Enzymatic Analysis","author":"Wolfe K","year":"1985","edition":"3"},{"key":"bibr23-jcbfm.1993.103","first-page":"R595","volume":"262","author":"Xia Y","year":"1992","journal-title":"Am J Physiol"},{"key":"bibr24-jcbfm.1993.103","volume-title":"Biostatistical Analysis","author":"Zar JH","year":"1974"}],"container-title":["Journal of Cerebral Blood Flow &amp; 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