{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T07:15:43Z","timestamp":1777792543561,"version":"3.51.4"},"reference-count":43,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[1995,7,1]],"date-time":"1995-07-01T00:00:00Z","timestamp":804556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cereb Blood Flow Metab"],"published-print":{"date-parts":[[1995,7]]},"abstract":"<jats:p>\n                    The relationship between local cerebral glucose utilization (LCMR\n                    <jats:sub>glc<\/jats:sub>\n                    ) and local CBF (LCBF) is known to be disturbed in regions surrounding an acute focal ischemic lesion\u2014areas that undergo repeated transient depolarizations. In this study, we evaluated the relationship between LCMR\n                    <jats:sub>glc<\/jats:sub>\n                    and LCBF in the acute focal ischemic penumbra to quantify metabolism\u2013flow uncoupling, and we related these findings to local electrophysiological measurements. A novel strategy utilizing three-dimensional (3D) autoradiographic image averaging yielded group 3D reconstructions of LCBF, LCMR\n                    <jats:sub>glc<\/jats:sub>\n                    , and the CMR\/CBF ratio. The distal right middle cerebral artery of Sprague-Dawley rats was occluded by laser-driven photothrombosis following administration of the photosensitizing dye rose bengal; this was coupled with permanent ipsilateral and 1-h contralateral common carotid artery occlusions. LCBF (n = 7) and LCMR\n                    <jats:sub>glc<\/jats:sub>\n                    (n = 7) were measured autoradiographically at 1.25 and 1.5\u20132 h postocclusion, respectively, in matched animal groups. Within the ischemic penumbra (defined as having LCBF of 20\u201340% of control or 0.23\u20130.47 ml g\n                    <jats:sup>\u22121<\/jats:sup>\n                    min\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), LCMR\n                    <jats:sub>glc<\/jats:sub>\n                    showed a heterogeneous pattern with values ranging from near normal to markedly increased. The resulting CMR\n                    <jats:sub>glc<\/jats:sub>\n                    \/CBF ratio in this zone was 234 \u00b1 100 \u03bcmol\/100 ml (mean \u00b1 SD), representing a severe degree of metabolism-flow dissociation when compared with the CMR\n                    <jats:sub>glc<\/jats:sub>\n                    \/CBF ratio of 51.0 \u00b1 28.7 \u03bcmol\/100 ml of the contralateral (normal) hemisphere. Metabolism\u2013flow uncoupling was confined to the ipsilateral cortex and was most prominent at the anterior and posterior coronal poles of the ischemic lesion. In the frontoparietal penumbra, where marked uncoupling was observed, sustained deflections of the DC potential were recorded, which increased significantly in duration over the initial 65 min postocclusion. Both the heterogeneous pattern of LCMR\n                    <jats:sub>glc<\/jats:sub>\n                    and the widespread distribution of increased CMR\n                    <jats:sub>glc<\/jats:sub>\n                    \/CBF ratio in the ischemic penumbra are thought to reflect the metabolic consequences of periinfarct depolarizations. Analysis of averaged 3D autoradiographic data sets provides a powerful means for assessing metabolism-flow uncoupling surrounding an ischemic focus.\n                  <\/jats:p>","DOI":"10.1038\/jcbfm.1995.70","type":"journal-article","created":{"date-parts":[[2011,4,8]],"date-time":"2011-04-08T13:18:52Z","timestamp":1302268732000},"page":"566-577","source":"Crossref","is-referenced-by-count":83,"title":["Three-Dimensional Image Analysis of Brain Glucose Metabolism-Blood Flow Uncoupling and its Electrophysiological Correlates in the Acute Ischemic Penumbra following Middle Cerebral Artery Occlusion"],"prefix":"10.1177","volume":"15","author":[{"given":"Tobias","family":"Back","sequence":"first","affiliation":[{"name":"Cerebral Vascular Disease Research Center, Department of Neurology, University of Miami School of Medicine, Miami, Florida, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weizhao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Cerebral Vascular Disease Research Center, Department of Neurology, University of Miami School of Medicine, Miami, Florida, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myron D.","family":"Ginsberg","sequence":"additional","affiliation":[{"name":"Cerebral Vascular Disease Research Center, Department of Neurology, University of Miami School of Medicine, Miami, Florida, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1995,7,1]]},"reference":[{"key":"bibr1-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1994.3"},{"key":"bibr2-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.25.2.494"},{"key":"bibr3-jcbfm.1995.70","first-page":"1761","volume":"20","author":"Back T","year":"1994","journal-title":"Soc Neurosci Abstr"},{"key":"bibr4-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1994.4"},{"key":"bibr5-jcbfm.1995.70","first-page":"58","volume":"2","author":"Ginsberg MD","year":"1990","journal-title":"Cerebrovasc Brain Metab Rev"},{"key":"bibr6-jcbfm.1995.70","first-page":"226","volume":"60","author":"Ginsberg MD","year":"1979","journal-title":"Acta Physiol Scand"},{"key":"bibr7-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.27.11.1042"},{"key":"bibr8-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410180410"},{"key":"bibr9-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1986.49"},{"key":"bibr10-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.37.1.11"},{"key":"bibr11-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410140307"},{"key":"bibr12-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1985.13"},{"key":"bibr13-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(83)90367-0"},{"key":"bibr14-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1992.53"},{"key":"bibr15-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1016\/0730-725X(94)00080-M"},{"key":"bibr16-jcbfm.1995.70","first-page":"H772","volume":"241","author":"Kuschinsky W","year":"1981","journal-title":"Am J Physiol"},{"key":"bibr17-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(86)90504-4"},{"key":"bibr18-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1944.7.6.359"},{"key":"bibr19-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.24.2.286"},{"key":"bibr20-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.14.1.22"},{"key":"bibr21-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.18.1.158"},{"key":"bibr22-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1986.108"},{"key":"bibr23-jcbfm.1995.70","first-page":"231","volume":"5","author":"Nedergaard M","year":"1985","journal-title":"J Cereb Blood Flow Metab"},{"key":"bibr24-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1986.74"},{"key":"bibr25-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-547620-1.50007-2"},{"key":"bibr26-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410110510"},{"key":"bibr27-jcbfm.1995.70","first-page":"125","volume-title":"Brain Dysfunction in Metabolic Disorders","author":"Reivich M","year":"1974"},{"key":"bibr28-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1969.27.2.296"},{"key":"bibr29-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1890.sp000321"},{"key":"bibr30-jcbfm.1995.70","doi-asserted-by":"crossref","first-page":"H59","DOI":"10.1152\/ajpcell.1978.234.3.C59","volume":"234","author":"Sakurada O","year":"1978","journal-title":"Am J Physiol"},{"key":"bibr31-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1980.tb06293.x"},{"key":"bibr32-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1126\/science.758688"},{"key":"bibr33-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1981.4"},{"key":"bibr34-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1977.tb10649.x"},{"key":"bibr35-jcbfm.1995.70","first-page":"III179","volume":"21","author":"Somjen GG","year":"1990","journal-title":"Stroke"},{"key":"bibr36-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1983.11"},{"key":"bibr37-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1985.31"},{"key":"bibr38-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410150608"},{"key":"bibr39-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.11.1.79"},{"key":"bibr40-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.24.6.864"},{"key":"bibr41-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1995.50"},{"key":"bibr42-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1109\/42.251130"},{"key":"bibr43-jcbfm.1995.70","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1995.69"}],"container-title":["Journal of Cerebral Blood Flow &amp; Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1038\/jcbfm.1995.70","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1038\/jcbfm.1995.70","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T20:07:24Z","timestamp":1777493244000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1038\/jcbfm.1995.70"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,7]]},"references-count":43,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1995,7]]}},"alternative-id":["10.1038\/jcbfm.1995.70"],"URL":"https:\/\/doi.org\/10.1038\/jcbfm.1995.70","relation":{},"ISSN":["0271-678X","1559-7016"],"issn-type":[{"value":"0271-678X","type":"print"},{"value":"1559-7016","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,7]]}}}