{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:50:32Z","timestamp":1791179432592,"version":"4.1.0"},"reference-count":37,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2008,7,2]],"date-time":"2008-07-02T00:00:00Z","timestamp":1214956800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cereb Blood Flow Metab"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:p>Cerebral arteriogenesis constitutes a promising therapeutic concept for cerebrovascular ischaemia; however, transcriptional profiles important for therapeutic target identification have not yet been investigated. This study aims at a comprehensive characterization of transcriptional and morphologic activation during early-phase collateral vessel growth in a rat model of adaptive cerebral arteriogenesis. Arteriogenesis was induced using a three-vessel occlusion (3-VO) rat model of nonischaemic cerebral hypoperfusion. Collateral tissue from growing posterior cerebral artery (PCA) and posterior communicating artery (Pcom) was selectively isolated avoiding contamination with adjacent tissue. We detected differential gene expression 24 h after 3-VO with 164 genes significantly deregulated. Expression patterns contained gene transcripts predominantly involved in proliferation, inflammation, and migration. By using scanning electron microscopy, morphologic activation of the PCA endothelium was detected. Furthermore, the PCA showed induced proliferation (PCNA staining) and CD68+ macrophage staining 24 h after 3-VO, resulting in a significant increase in diameter within 7 days after 3-VO, confirming the arteriogenic phenotype. Analysis of molecular annotations and networks associated with differentially expressed genes revealed that early-phase cerebral arteriogenesis is characterised by the expression of protease inhibitors. These results were confirmed by quantitative real-time reverse transcription-PCR, and in situ hybridisation localised the expression of tissue inhibitor of metalloproteinase-1 (TIMP-1) and kininogen to collateral arteries, showing that TIMP-1 and kininogen might be molecular markers for early-phase cerebral arteriogenesis.<\/jats:p>","DOI":"10.1038\/jcbfm.2008.69","type":"journal-article","created":{"date-parts":[[2008,7,2]],"date-time":"2008-07-02T04:14:16Z","timestamp":1214972056000},"page":"1811-1823","source":"Crossref","is-referenced-by-count":23,"title":["Induction of Cerebral Arteriogenesis Leads to Early-Phase Expression of Protease Inhibitors in Growing Collaterals of the Brain"],"prefix":"10.1177","volume":"28","author":[{"given":"Philipp","family":"Hillmeister","sequence":"first","affiliation":[{"name":"Research Group for Experimental and Clinical Arteriogenesis, Department of Internal Medicine\/Cardiology, (CC13), CCR\u2014Center for Cardiovascular Research, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin E","family":"Lehmann","sequence":"additional","affiliation":[{"name":"Research Group for Experimental and Clinical Arteriogenesis, Department of Internal Medicine\/Cardiology, (CC13), CCR\u2014Center for Cardiovascular Research, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Bondke","sequence":"additional","affiliation":[{"name":"Research Group for Experimental and Clinical Arteriogenesis, Department of Internal Medicine\/Cardiology, (CC13), CCR\u2014Center for Cardiovascular Research, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Berlin, Germany"},{"name":"Charit\u00e9 Universit\u00e4tsmedizin Berlin, Center for Preclinical Studies (CC2), Institute of Physiology, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henning","family":"Witt","sequence":"additional","affiliation":[{"name":"CCR\u2014Center for Cardiovascular Research, Max-Planck-Institute for Molecular Genetics, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Duelsner","sequence":"additional","affiliation":[{"name":"Research Group for Experimental and Clinical Arteriogenesis, Department of Internal Medicine\/Cardiology, (CC13), CCR\u2014Center for Cardiovascular Research, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clemens","family":"Gruber","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Institute of Integrative Neuroanatomy, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-J\u00f6rg","family":"Busch","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine (Cardiology), University of Freiburg, Freiburg i. Br., Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Jankowski","sequence":"additional","affiliation":[{"name":"Medical Clinic IV, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Ruiz-Noppinger","sequence":"additional","affiliation":[{"name":"CCR\u2014Center for Cardiovascular Research, Max-Planck-Institute for Molecular Genetics, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantin-Alexander","family":"Hossmann","sequence":"additional","affiliation":[{"name":"Max-Planck Institute for Neurological Research, Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivo R","family":"Buschmann","sequence":"additional","affiliation":[{"name":"Research Group for Experimental and Clinical Arteriogenesis, Department of Internal Medicine\/Cardiology, (CC13), CCR\u2014Center for Cardiovascular Research, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Berlin, Germany"},{"name":"Department of Internal Medicine (Cardiology), University of Freiburg, Freiburg i. Br., Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2008,7,2]]},"reference":[{"key":"bibr1-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19990428)407:1<130::AID-CNE10>3.0.CO;2-8"},{"key":"bibr2-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119877"},{"key":"bibr3-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0206087"},{"key":"bibr4-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.050706"},{"key":"bibr5-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1097\/01.WCB.0000057741.00152.E4"},{"key":"bibr6-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(200002)190:3<338::AID-PATH594>3.0.CO;2-7"},{"key":"bibr7-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000074209.17561.99"},{"key":"bibr8-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.2000.1137"},{"key":"bibr9-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1023\/B:MCBI.0000044389.65590.57"},{"key":"bibr10-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2006.085"},{"key":"bibr11-jcbfm.2008.69","first-page":"37","volume":"341","author":"Chavakis T","year":"2006","journal-title":"Methods Mol Biol"},{"key":"bibr12-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1046\/j.1538-7836.2003.00025.x"},{"key":"bibr13-jcbfm.2008.69","first-page":"183","volume":"28","author":"Coyle P","year":"1991","journal-title":"Blood Vessels"},{"key":"bibr14-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1161\/hh2101.098613"},{"key":"bibr15-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2003-4-5-p3"},{"key":"bibr16-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1161\/hc0302.102142"},{"key":"bibr17-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0012-07.2007"},{"key":"bibr18-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01098.2001"},{"key":"bibr19-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1161\/hs1201.099892"},{"key":"bibr20-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.5.12.1547"},{"key":"bibr21-jcbfm.2008.69","first-page":"291","volume-title":"Collateral circulation","author":"Hossmann K-A","year":"1993"},{"key":"bibr22-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00035.2004"},{"key":"bibr23-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2003.09.033"},{"key":"bibr24-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000111123.10730.01"},{"key":"bibr25-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(03)00478-2"},{"key":"bibr26-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199805110-00012"},{"key":"bibr27-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012493808790"},{"key":"bibr28-jcbfm.2008.69","first-page":"519","volume":"41","author":"McCracken JS","year":"1979","journal-title":"Lab Invest"},{"key":"bibr29-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.83.6.2048"},{"key":"bibr30-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2006.017"},{"key":"bibr31-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00398.2004"},{"key":"bibr32-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.14.5.832"},{"key":"bibr33-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1007\/1-4020-8126-X_13"},{"key":"bibr34-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-006-0097-6"},{"key":"bibr35-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404880101"},{"key":"bibr36-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp019432"},{"key":"bibr37-jcbfm.2008.69","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(00)00206-6"}],"container-title":["Journal of Cerebral Blood Flow &amp; Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1038\/jcbfm.2008.69","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1038\/jcbfm.2008.69","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1038\/jcbfm.2008.69","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T20:09:27Z","timestamp":1777493367000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1038\/jcbfm.2008.69"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7,2]]},"references-count":37,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2008,11]]}},"alternative-id":["10.1038\/jcbfm.2008.69"],"URL":"https:\/\/doi.org\/10.1038\/jcbfm.2008.69","relation":{},"ISSN":["0271-678X","1559-7016"],"issn-type":[{"value":"0271-678X","type":"print"},{"value":"1559-7016","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,7,2]]}}}