{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T04:22:18Z","timestamp":1778818938013,"version":"3.51.4"},"reference-count":25,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2010,7,28]],"date-time":"2010-07-28T00:00:00Z","timestamp":1280275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Cereb Blood Flow Metab"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:p>\n                    An\n                    <jats:sup>15<\/jats:sup>\n                    O-labeled water (H\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>15<\/jats:sup>\n                    O) steady-state method for quantitative measurement of cerebral blood flow ( CBF), which is less stressful to small animals with a few point blood sampling, was developed. After a simulation using a dose meter to achieve stable H2\n                    <jats:sup>15<\/jats:sup>\n                    O radioactivity in the blood with a multiprogramming syringe pump programmed for slowly increasing injection volume, 10 rats were studied with the injection method. Arterial blood was sampled every minute during 6-minute positron emission tomography (PET) scans. After the PET scan, N-isopropyl- p-[\n                    <jats:sup>125<\/jats:sup>\n                    I]-iodoamphetamine (\n                    <jats:sup>125<\/jats:sup>\n                    I-IMP) was injected into the same rat to measure CBF using the autoradiography method based on a microsphere model. Regions of interest were placed on the whole brain in H2\n                    <jats:sup>15<\/jats:sup>\n                    O-PET and\n                    <jats:sup>125<\/jats:sup>\n                    I-IMP-autoradiography images, and CBF values calculated from both methods were compared. Radioactivity in the dose meter achieved equilibrium ~1 minute after starting the H\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>15<\/jats:sup>\n                    O injection. In rat studies, radioactivity in the blood and brain rapidly achieved equilibrium at 2 minutes after administration. The correlation of CBF values of H\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>15<\/jats:sup>\n                    O PET (49.2 \u00b1 5.4 mL per 100 g per minute) and those of\n                    <jats:sup>125<\/jats:sup>\n                    I-IMP autoradiography (49.1 \u00b1 5.2 mL per 100 g per minute) was excellent ( y = 1.01 x \u22120.37, r\n                    <jats:sup>2<\/jats:sup>\n                    = 0.97). The H\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>15<\/jats:sup>\n                    O steady-state method with a continuously increasing injection is useful for CBF measurement in small animal studies, especially when multiple scans are required in the same animal.\n                  <\/jats:p>","DOI":"10.1038\/jcbfm.2010.122","type":"journal-article","created":{"date-parts":[[2010,7,28]],"date-time":"2010-07-28T04:07:39Z","timestamp":1280290059000},"page":"527-534","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Development of an H\n                    <sub>2<\/sub>\n                    <sup>15<\/sup>\n                    O Steady-State Method Combining a Bolus and Slow Increasing Injection with a Multiprogramming Syringe Pump"],"prefix":"10.1177","volume":"31","author":[{"given":"Masato","family":"Kobayashi","sequence":"first","affiliation":[{"name":"Biomedical Imaging Research Center, University of Fukui, Fukui, Japan"},{"name":"School of Health Sciences, College of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasushi","family":"Kiyono","sequence":"additional","affiliation":[{"name":"Biomedical Imaging Research Center, University of Fukui, Fukui, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rikiya","family":"Maruyama","sequence":"additional","affiliation":[{"name":"Biomedical Imaging Research Center, University of Fukui, Fukui, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Mori","sequence":"additional","affiliation":[{"name":"Biomedical Imaging Research Center, University of Fukui, Fukui, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiichi","family":"Kawai","sequence":"additional","affiliation":[{"name":"Biomedical Imaging Research Center, University of Fukui, Fukui, Japan"},{"name":"School of Health Sciences, College of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidehiko","family":"Okazawa","sequence":"additional","affiliation":[{"name":"Biomedical Imaging Research Center, University of Fukui, Fukui, Japan"},{"name":"Research and Education Program for Life Science, University of Fukui, Fukui, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2010,7,28]]},"reference":[{"key":"bibr1-jcbfm.2010.122","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1993.6"},{"key":"bibr2-jcbfm.2010.122","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199704000-00009"},{"key":"bibr3-jcbfm.2010.122","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-8051(00)00138-4"},{"key":"bibr4-jcbfm.2010.122","first-page":"641","volume":"34","author":"Di-Rocco 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