{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T07:28:32Z","timestamp":1777793312060,"version":"3.51.4"},"reference-count":39,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2012,1,4]],"date-time":"2012-01-04T00:00:00Z","timestamp":1325635200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Cereb Blood Flow Metab"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:p>\n                    This positron emission tomography (PET) study aimed to further define selectivity of [\n                    <jats:sup>11<\/jats:sup>\n                    C]Ro15-4513 binding to the GABAR\u03b15 relative to the GABAR\u03b11 benzodiazepine receptor subtype. The impact of zolpidem, a GABAR\u03b11-selective agonist, on [\n                    <jats:sup>11<\/jats:sup>\n                    C]Ro15-4513, which shows selectivity for GABAR\u03b15, and the nonselective benzodiazepine ligand [\n                    <jats:sup>11<\/jats:sup>\n                    C]flumazenil binding was assessed in humans. Compartmental modelling of the kinetics of [\n                    <jats:sup>11<\/jats:sup>\n                    C]Ro15-4513 time-activity curves was used to describe distribution volume ( V\n                    <jats:sub>T<\/jats:sub>\n                    ) differences in regions populated by different GABA receptor subtypes. Those with low \u03b15 were best fitted by one-tissue compartment models; and those with high \u03b15 required a more complex model. The heterogeneity between brain regions suggested spectral analysis as a more appropriate method to quantify binding as it does not a priori specify compartments. Spectral analysis revealed that Zolpidem caused a significant V\n                    <jats:sub>T<\/jats:sub>\n                    decrease (~10%) in [\n                    <jats:sup>11<\/jats:sup>\n                    C]flumazenil, but no decrease in [\n                    <jats:sup>11<\/jats:sup>\n                    C]Ro15-4513 binding. Further analysis of [\n                    <jats:sup>11<\/jats:sup>\n                    C]Ro15-4513 kinetics revealed additional frequency components present in regions containing both \u03b11 and \u03b15 subtypes compared with those containing only \u03b11. Zolpidem reduced one component (mean \u00b1 s.d.: 71% \u00b1 41%), presumed to reflect \u03b11-subtype binding, but not another (13% \u00b1 22%), presumed to reflect \u03b15. The proposed method for [\n                    <jats:sup>11<\/jats:sup>\n                    C]Ro15-4513 analysis may allow more accurate selective binding assays and estimation of drug occupancy for other nonselective ligands.\n                  <\/jats:p>","DOI":"10.1038\/jcbfm.2011.177","type":"journal-article","created":{"date-parts":[[2012,1,4]],"date-time":"2012-01-04T10:28:54Z","timestamp":1325672934000},"page":"731-744","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":33,"title":["Characterisation of the Contribution of the GABA-Benzodiazepine \u03b11 Receptor Subtype to [\n                    <sup>11<\/sup>\n                    C]Ro15-4513 PET Images"],"prefix":"10.1177","volume":"32","author":[{"given":"James FM","family":"Myers","sequence":"first","affiliation":[{"name":"Psychopharmacology Unit, University of Bristol, Bristol, 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