{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T09:21:08Z","timestamp":1789464068753,"version":"build-2803163510"},"reference-count":37,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2012,5,2]],"date-time":"2012-05-02T00:00:00Z","timestamp":1335916800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Cereb Blood Flow Metab"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:p>Preclinical trials confirmed the potential of mesenchymal stromal cells (MSCs) to improve functional recovery after experimental stroke. Beneficial effects of MSCs are often attributed to their immunosuppressive\/immunomodulatory functions. Surprisingly, the influence of MSCs on the immune system after stroke is poorly understood, but requires special consideration because cerebral ischemia is associated with stroke-induced immunodepression that predisposes to bacterial infections with increased mortality. In this study, we intravenously transplanted syngeneic murine bone marrow-derived MSCs (mMSCs) into C57BL\/6 mice at 6\u2009hours after transient middle cerebral artery occlusion (MCAo; 60\u2009minutes) to investigate the impact of MSCs on stroke-induced immunodepression. Transplantation of syngeneic splenocytes or phosphate-buffered saline (PBS) served as controls. An immune status was determined by flow cytometry on days 3 and 14 after MCAo, which did not reveal any negative effects of cell transplantation on stroke-induced immunodepression. Although our mMSCs were found to exert immunosuppressive effects in vitro, stroke-mediated immune cell dysfunction was not altered by mMSCs in ex-vivo stimulation assays with lipopolysaccharide or concanavalin A. Moreover, systemic inflammatory cytokine levels (interleukin-6, tumor necrosis factor \u03b1, interferon \u03b3, monocyte chemoattractant protein-1) remained unchanged in the sera of mice after cerebral ischemia and cell transplantation. These results reduce safety concerns about MSC administration in ongoing clinical stroke trials.<\/jats:p>","DOI":"10.1038\/jcbfm.2012.55","type":"journal-article","created":{"date-parts":[[2012,5,2]],"date-time":"2012-05-02T04:06:24Z","timestamp":1335931584000},"page":"1578-1588","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":40,"title":["Immune Effects of Mesenchymal Stromal Cells in Experimental Stroke"],"prefix":"10.1177","volume":"32","author":[{"given":"Franziska","family":"Scheibe","sequence":"first","affiliation":[{"name":"Department of Neuropsychiatry and Laboratory of Molecular Psychiatry, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Berlin, Germany"},{"name":"Department of Experimental Neurology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Berlin, Germany"},{"name":"Berlin-Brandenburg Center for Regenerative Therapies, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Berlin, 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