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Considering its widespread availability and low immunogenicity human umbilical cord blood (HUCB) is a particularly attractive stem cell source. Our goal was to investigate the neurorestorative potential of cryopreserved HUCB mononuclear cells (MNC) after permanent middle cerebral artery occlusion (MCAO) in spontaneously hypertensive rats (SHR). Human umbilical cord blood MNC or vehicle solution was administered intravenously 24\u2009hours after MCAO. Experimental groups were as follows: (1) quantitative polymerase chain reaction (PCR) of host-derived growth factors up to 48\u2009hours after stroke; (2) immunohistochemical analysis of astroglial scarring; (3) magnetic resonance imaging (MRI) and weekly behavioral tests for 2 months after stroke. Long-term functional outcome and lesion development on MRI were not beneficially influenced by HUCB MNC therapy. Furthermore, HUCB MNC treatment did not change local growth factor levels and glial scarring extent. In summary, we could not demonstrate neurorestorative properties of HUCB MNC after stroke in SHR. Our results advise caution regarding a prompt translation of cord blood therapy into clinical stroke trials as long as deepened knowledge about its precise modes of action is missing.<\/jats:p>","DOI":"10.1038\/jcbfm.2013.185","type":"journal-article","created":{"date-parts":[[2013,10,30]],"date-time":"2013-10-30T04:55:38Z","timestamp":1383108938000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":44,"title":["Transplantation of Cryopreserved Human Umbilical Cord Blood Mononuclear Cells Does Not Induce Sustained Recovery after Experimental Stroke in Spontaneously Hypertensive Rats"],"prefix":"10.1177","volume":"34","author":[{"given":"Gesa","family":"Weise","sequence":"first","affiliation":[{"name":"Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany"},{"name":"Translational Center for Regenerative Medicine, University of Leipzig, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlene","family":"Lorenz","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"P\u00f6sel","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute","family":"Maria Riegelsberger","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"St\u00f6rbeck","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manja","family":"Kamprad","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany"},{"name":"Institute for Clinical Immunology, University of Leipzig, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Kranz","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel-Christoph","family":"Wagner","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany"},{"name":"Translational Center for Regenerative Medicine, University of Leipzig, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Boltze","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany"},{"name":"Translational Center for Regenerative Medicine, University of Leipzig, Leipzig, Germany"},{"name":"Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2013,10,30]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199805150-00024"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa041469"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3727\/096368911X586783"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3727\/096368911X589609"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000141680.49960.d7"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/hs1101.098367"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2005.14.595"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aanat.2011.03.005"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2009.10.003"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-012-1401-0"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2004.02420.x"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2005.06.091"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.09.056"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.55782\/ane-2009-1728"},{"key":"e_1_3_3_16_2","first-page":"50","article-title":"The effect of freezing on the recovery and expansion of umbilical cord blood hematopoietic stem cells","volume":"7","author":"Beshlawy AE","year":"2009","unstructured":"Beshlawy AE, Metwally HG, Khalek KA, Hammoud RF, Mousa SM. 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