{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:44:53Z","timestamp":1790739893637,"version":"4.1.0"},"reference-count":40,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2014,1,22]],"date-time":"2014-01-22T00:00:00Z","timestamp":1390348800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Cereb Blood Flow Metab"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:p>\n                    To assess the capability of\n                    <jats:sup>18<\/jats:sup>\n                    F-2-tert-butyl-4-chloro-5-{6-[2-(2-fluoroethoxy)-ethoxy]-pyridin-3-ylmethoxy}-2H-pyridazin-3-one (\n                    <jats:sup>18<\/jats:sup>\n                    F-BCPP-EF), a novel positron emission tomography (PET) probe for mitochondrial complex I (MC-I) activity, as a specific marker of ischemia-induced neuronal death without being disturbed by inflammation, translational research was conducted using an animal PET in ischemic brains of Cynomolgus monkeys ( Macaca fascicularis). Focal ischemia was induced by the right middle cerebral artery occlusion for 3\u2009hours, then PET scans were conducted at Day-7 with\n                    <jats:sup>15<\/jats:sup>\n                    O-gases for regional cerebral blood flow (rCBF) and regional cerebral metabolism of oxygen (rCMRO\n                    <jats:sub>2<\/jats:sub>\n                    ), and\n                    <jats:sup>18<\/jats:sup>\n                    F-BCPP-EF for MC-I with arterial blood sampling. On Day-8, the additional PET scans conducted with\n                    <jats:sup>11<\/jats:sup>\n                    C-flumazenil (\n                    <jats:sup>11<\/jats:sup>\n                    C-FMZ) for central-type benzodiazepine receptors,\n                    <jats:sup>11<\/jats:sup>\n                    C-PBR28 for translocator protein, and\n                    <jats:sup>18<\/jats:sup>\n                    F-fluoro-2-deoxy-D-glucose (\n                    <jats:sup>18<\/jats:sup>\n                    F-FDG) for regional cerebral metabolic rate of glucose (rCMRglc). The total distribution volume ( V\n                    <jats:sub>T<\/jats:sub>\n                    ) values of\n                    <jats:sup>18<\/jats:sup>\n                    F-BCPP-EF showed the significant reduction in MC-I activity in the damaged area at Day-7. When correlated with rCBF and rCMRO\n                    <jats:sub>2<\/jats:sub>\n                    , the V\n                    <jats:sub>T<\/jats:sub>\n                    values of\n                    <jats:sup>18<\/jats:sup>\n                    F-BCPP-EF provided better correlation with rCMRO\n                    <jats:sub>2<\/jats:sub>\n                    than with rCBF. In the inflammatory regions (region of interest, ROI\n                    <jats:sub>PBR<\/jats:sub>\n                    ) of the ischemic hemisphere detected with\n                    <jats:sup>11<\/jats:sup>\n                    C-PBR28, higher\n                    <jats:sup>18<\/jats:sup>\n                    F-FDG uptake and lower V\n                    <jats:sub>T<\/jats:sub>\n                    of\n                    <jats:sup>18<\/jats:sup>\n                    F-BCPP-EF,\n                    <jats:sup>11<\/jats:sup>\n                    C-FMZ, and rCMRO\n                    <jats:sub>2<\/jats:sub>\n                    than those in normal contralateral hemisphere were observed. These results strongly suggested that\n                    <jats:sup>18<\/jats:sup>\n                    F-BCPP-EF could discriminate the neuronal damaged areas with neuroinflammation, where\n                    <jats:sup>18<\/jats:sup>\n                    F-FDG could not owing to its high uptake into the activated microglia.\n                  <\/jats:p>","DOI":"10.1038\/jcbfm.2014.5","type":"journal-article","created":{"date-parts":[[2014,1,22]],"date-time":"2014-01-22T04:02:35Z","timestamp":1390363355000},"page":"708-714","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":38,"title":["PET Imaging of Ischemia-Induced Impairment of Mitochondrial Complex I Function in Monkey Brain"],"prefix":"10.1177","volume":"34","author":[{"given":"Hideo","family":"Tsukada","sequence":"first","affiliation":[{"name":"Central Research Laboratory, Hamamatsu Photonics K.K., Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Ohba","sequence":"additional","affiliation":[{"name":"Central Research Laboratory, Hamamatsu Photonics K.K., Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shingo","family":"Nishiyama","sequence":"additional","affiliation":[{"name":"Central Research Laboratory, Hamamatsu Photonics K.K., Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masakatsu","family":"Kanazawa","sequence":"additional","affiliation":[{"name":"Central Research Laboratory, Hamamatsu Photonics K.K., Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeharu","family":"Kakiuchi","sequence":"additional","affiliation":[{"name":"Central Research Laboratory, Hamamatsu Photonics K.K., Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norihiro","family":"Harada","sequence":"additional","affiliation":[{"name":"Central Research Laboratory, Hamamatsu Photonics K.K., Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2014,1,22]]},"reference":[{"key":"bibr1-jcbfm.2014.5","doi-asserted-by":"publisher","DOI":"10.1038\/74994"},{"key":"bibr2-jcbfm.2014.5","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2012.32"},{"key":"bibr3-jcbfm.2014.5","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302743200"},{"key":"bibr4-jcbfm.2014.5","first-page":"1409","volume":"41","author":"Takamatsu H","year":"2000","journal-title":"J Nucl 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