{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T10:54:21Z","timestamp":1783421661068,"version":"3.54.6"},"reference-count":39,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2014,4,30]],"date-time":"2014-04-30T00:00:00Z","timestamp":1398816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Cereb Blood Flow Metab"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:p>\n                    Surgical flow augmentation for treatment of cerebral hemodynamic impairment remains controversial. Here, we investigated the benefit of endothelial progenitor cell (EPC) treatment in a rat model of chronic cerebral hypoperfusion. At repeated time points after 3-vessel occlusion (3-VO), animals were treated with 1 \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    Dil-labeled (a) ex vivo-expanded embryonic-EPC (e-EPC), (b) cyclic AMP-differentiated embryonic-endothelial progenitor-derived cells (e-EPDC as biologic control) or, (c) saline. The cerebrovascular reserve capacity (CVRC) was assessed immediately before and on days 7 and 21 after 3-VO. Structural effects were assessed by latex perfusion, immunohistochemistry, and intravital fluorescence video microscopy on day 21. Three-vessel occlusion resulted in a significant impairment of the CVRC with better functional recovery after treatment with e-EPC (16.4 \u00b1 8%) compared with e-EPDC (3.7 \u00b1 8%) or saline (6.4 \u00b1 9%) by day 21 ( P&lt;0.05), which was paralleled by a significant increase in the vessel diameters of the anterior Circle of Willis, a significantly higher number of leptomeningeal anastomoses and higher parenchymal capillary density in e-EPC-treated animals. Interestingly, despite in vivo interaction of e-EPC with the cerebral endothelium, e-EPC incorporation into the cerebral vasculature was not observed. Our results suggest that EPC may serve as a novel therapeutic agent in clinical trials for nonsurgical treatment of chronic cerebral hemodynamic impairment.\n                  <\/jats:p>","DOI":"10.1038\/jcbfm.2014.78","type":"journal-article","created":{"date-parts":[[2014,4,30]],"date-time":"2014-04-30T04:00:58Z","timestamp":1398830458000},"page":"1297-1305","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":26,"title":["Endothelial Progenitor Cells Augment Collateralization and Hemodynamic Rescue in a Model of Chronic Cerebral Ischemia"],"prefix":"10.1177","volume":"34","author":[{"given":"Nils","family":"Hecht","sequence":"first","affiliation":[{"name":"Department of Neurosurgery and Center for Stroke Research Berlin (CSB), Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulf C","family":"Schneider","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery and Center for Stroke Research Berlin (CSB), Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus","family":"Czabanka","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery and Center for Stroke Research Berlin (CSB), Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Vinci","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Universit\u00e4tsmedizin Mannheim, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonis K","family":"Hatzopoulos","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Vajkoczy","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery and Center for Stroke Research Berlin (CSB), Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Woitzik","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery and Center for Stroke Research Berlin (CSB), Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Berlin, 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