{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T07:22:54Z","timestamp":1777792974188,"version":"3.51.4"},"reference-count":40,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2015,3,18]],"date-time":"2015-03-18T00:00:00Z","timestamp":1426636800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Cereb Blood Flow Metab"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:p>Neurofibromatosis type 1 (NF1) is a single-gene disorder affecting neurologic function in humans. The NF1+\/\u2013 mouse model with germline mutation of the NF1 gene presents with deficits in learning, attention, and motor coordination, very similar to NF1 patients. The present study performed brain perfusion single-photon emission computed tomography (SPECT) in NF1+\/\u2013 mice to identify possible perfusion differences as surrogate marker for altered cerebral activity in NF1. Cerebral perfusion was measured with hexamethyl-propyleneamine oxime (HMPAO) SPECT in NF1+\/\u2013 mice and their wild-type littermates longitudinally at juvenile age and at young adulthood. Histology and immunohistochemistry were performed to test for structural changes. There was increased HMPAO uptake in NF1 mice in the amygdala at juvenile age, which reduced to normal levels at young adulthood. There was no genotype effect on thalamic HMPAO uptake, which was confirmed by ex vivo measurements of F-18-fluorodeoxyglucose uptake in the thalamus. Morphologic analyses showed no major structural abnormalities. However, there was some evidence of increased density of microglial somata in the amygdala of NF1-deficient mice. In conclusion, there is evidence of increased perfusion and increased density of microglia in juvenile NF1 mice specifically in the amygdala, both of which might be associated with altered synaptic plasticity and, therefore, with cognitive deficits in NF1.<\/jats:p>","DOI":"10.1038\/jcbfm.2015.43","type":"journal-article","created":{"date-parts":[[2015,3,18]],"date-time":"2015-03-18T04:54:33Z","timestamp":1426654473000},"page":"1304-1312","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Perfusion Single Photon Emission Computed Tomography in a Mouse Model of Neurofibromatosis Type 1: Towards a Biomarker of Neurologic Deficits"],"prefix":"10.1177","volume":"35","author":[{"given":"Ivayla","family":"Apostolova","sequence":"first","affiliation":[{"name":"Department of Nuclear Medicine, University Medicine Charit\u00e9 Berlin, Berlin, Germany"},{"name":"Department of Radiology and Nuclear Medicine, University Hospital Magdeburg, Otto-von-Guericke University, Magdeburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dagmara","family":"Niedzielska","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thorsten","family":"Derlin","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, Hannover Medical School, Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva J","family":"Koziolek","sequence":"additional","affiliation":[{"name":"Department of Diagnostic and Interventional Radiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Holger","family":"Amthauer","sequence":"additional","affiliation":[{"name":"Department of Radiology and Nuclear Medicine, University Hospital Magdeburg, Otto-von-Guericke University, Magdeburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benedikt","family":"Salmen","sequence":"additional","affiliation":[{"name":"Neuroscience Research Center, University Medicine Charit\u00e9 Berlin, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens","family":"Pahnke","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, Oslo University Hospital (OUS), University of Oslo (UiO), Oslo, Norway"},{"name":"LIED, University of L\u00fcbeck, L\u00fcbeck, Germany"},{"name":"Leibniz Institute of Plant Biochemistry, Halle, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Winfried","family":"Brenner","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, University Medicine Charit\u00e9 Berlin, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor F","family":"Mautner","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralph","family":"Buchert","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, University Medicine Charit\u00e9 Berlin, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2015,3,18]]},"reference":[{"key":"bibr1-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1016\/S1090-3798(03)00039-4"},{"key":"bibr2-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2008.061051"},{"key":"bibr3-jcbfm.2015.43","first-page":"1699","volume":"25","author":"Friedrich RE","year":"2005","journal-title":"Anticancer Res"},{"key":"bibr4-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/nos287"},{"key":"bibr5-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1016\/j.braindev.2009.12.008"},{"key":"bibr6-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1007\/s12311-010-0217-2"},{"key":"bibr7-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1007\/s11910-003-0064-3"},{"key":"bibr8-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.36338"},{"key":"bibr9-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1038\/nature711"},{"key":"bibr10-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1038\/ng0794-353"},{"key":"bibr11-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1006\/scbi.1996.0037"},{"key":"bibr12-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1038\/sj.neo.7900249"},{"key":"bibr13-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1177\/088307380201700813"},{"key":"bibr14-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4914(02)00008-4"},{"key":"bibr15-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00634-7"},{"key":"bibr16-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3822"},{"key":"bibr17-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.17.6010"},{"key":"bibr18-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2011.03.004"},{"key":"bibr19-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2011.03.005"},{"key":"bibr20-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.57.12.1479"},{"key":"bibr21-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1097\/MNM.0b013e3282f1bbf5"},{"key":"bibr22-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1177\/088307389701200807"},{"key":"bibr23-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1007\/s11307-013-0702-3"},{"key":"bibr24-jcbfm.2015.43","first-page":"21","volume":"43","author":"Herholz K","year":"2002","journal-title":"J Nucl Med"},{"key":"bibr25-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awr179"},{"key":"bibr26-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2012.08.038"},{"key":"bibr27-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1038\/ng0397-281"},{"key":"bibr28-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/197381"},{"key":"bibr29-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2005.07.014"},{"key":"bibr30-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.3389\/neuro.05.001.2008"},{"key":"bibr31-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.2174\/156720511797633179"},{"key":"bibr32-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-2506"},{"key":"bibr33-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2013.08.007"},{"key":"bibr34-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.1546"},{"key":"bibr35-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.2006.108134"},{"key":"bibr36-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.46.6.1660"},{"key":"bibr37-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-007-7056-4"},{"key":"bibr38-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.4161\/cib.4.2.14506"},{"key":"bibr39-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3678"},{"key":"bibr40-jcbfm.2015.43","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/425845"}],"container-title":["Journal of Cerebral Blood Flow &amp; Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1038\/jcbfm.2015.43","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1038\/jcbfm.2015.43","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1038\/jcbfm.2015.43","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T20:08:45Z","timestamp":1777493325000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1038\/jcbfm.2015.43"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,18]]},"references-count":40,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.1038\/jcbfm.2015.43"],"URL":"https:\/\/doi.org\/10.1038\/jcbfm.2015.43","relation":{},"ISSN":["0271-678X","1559-7016"],"issn-type":[{"value":"0271-678X","type":"print"},{"value":"1559-7016","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,18]]}}}