{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T02:15:48Z","timestamp":1790129748308,"version":"4.0.1"},"reference-count":46,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T00:00:00Z","timestamp":1673395200000},"content-version":"vor","delay-in-days":4850,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["li.uscap.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Laboratory Investigation"],"published-print":{"date-parts":[[2009,10]]},"DOI":"10.1038\/labinvest.2009.83","type":"journal-article","created":{"date-parts":[[2009,8,31]],"date-time":"2009-08-31T10:27:02Z","timestamp":1251714422000},"page":"1140-1151","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":66,"title":["Hepatic damage associated with dengue-2 virus replication in liver cells of BALB\/c mice"],"prefix":"10.1016","volume":"89","author":[{"given":"Marciano Viana","family":"Paes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrique Leonel","family":"Lenzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Cristina Martins","family":"Nogueira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard James","family":"Nuovo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c2ngela Teixeira","family":"Pinh\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ester Maria","family":"Mota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Alberto","family":"Bas\u00edlio-de-Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermann","family":"Schatzmayr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ortrud Monika","family":"Barth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ada Maria","family":"de Barcelos Alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1038\/labinvest.2009.83_bib1","series-title":"Fields Virology","first-page":"991","article-title":"Flaviviridae: the viruses and theireplication","author":"Lindenbach","year":"2001"},{"key":"10.1038\/labinvest.2009.83_bib2","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1128\/CMR.11.3.480","article-title":"Dengue and dengue hemorrhagic fever","volume":"11","author":"Gubler","year":"1998","journal-title":"Clin Microbiol Rev"},{"key":"10.1038\/labinvest.2009.83_bib3","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1053\/j.semdp.2007.07.002","article-title":"The pathology of dengue hemorrhagic fever","volume":"24","author":"Leong","year":"2007","journal-title":"Semin Diagn Pathol"},{"key":"10.1038\/labinvest.2009.83_bib4","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.ddmod.2006.03.014","article-title":"Models of dengue virus infection","volume":"3","author":"Bente","year":"2006","journal-title":"Drug Discov Today Dis Models"},{"key":"10.1038\/labinvest.2009.83_bib5","doi-asserted-by":"crossref","first-page":"408","DOI":"10.4269\/ajtmh.1991.45.408","article-title":"Demonstration of yellow fever and dengue antigens in formalin-fixed paraffin-embedded human liver by immunohistochemical analysis","volume":"45","author":"Hall","year":"1991","journal-title":"Am J Trop Med Hyg"},{"key":"10.1038\/labinvest.2009.83_bib6","doi-asserted-by":"crossref","first-page":"720","DOI":"10.4269\/ajtmh.1999.61.720","article-title":"Detection of dengue virus RNA by reverse transcription-polymerase chain reaction in the liver and lymphoid organs but not in the brain in fatal human infection","volume":"61","author":"Rosen","year":"1999","journal-title":"Am J Trop Med Hyg"},{"key":"10.1038\/labinvest.2009.83_bib7","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1016\/S0046-8177(99)90230-7","article-title":"Report of a fatal case of dengue infection with hepatitis: demonstration of dengue antigens in hepatocytes and liver apoptosis","volume":"30","author":"Couvelard","year":"1999","journal-title":"Hum Pathol"},{"key":"10.1038\/labinvest.2009.83_bib8","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/s004280000329","article-title":"Liver histopathology and biological correlates in five cases of fatal dengue fever in Vietnamese children","volume":"438","author":"Huerre","year":"2001","journal-title":"Virchows Arch"},{"key":"10.1038\/labinvest.2009.83_bib9","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1590\/S1413-86702005000400012","article-title":"Pathologic study of a fatal case of dengue-3 virus infection in Rio de Janeiro","volume":"9","author":"Bas\u00edlio-de-Oliveira","year":"2005","journal-title":"Braz J Infect Dis"},{"key":"10.1038\/labinvest.2009.83_bib10","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.antiviral.2008.06.010","article-title":"Mouse models of dengue virus infection and disease","volume":"80","author":"Yauch","year":"2008","journal-title":"Antiviral Res"},{"key":"10.1038\/labinvest.2009.83_bib11","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.virol.2005.04.042","article-title":"Liver injury and viremia in mice infected with dengue-2 virus","volume":"338","author":"Paes","year":"2005","journal-title":"Virology"},{"key":"10.1038\/labinvest.2009.83_bib12","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1590\/S0036-46651995000600006","article-title":"Dengue type 2 outbreak in the south of the state of Bahia, Brazil: laboratorial and epidemiological studies","volume":"37","author":"Nogueira","year":"1995","journal-title":"Rev Ins Med Trop SP"},{"key":"10.1038\/labinvest.2009.83_bib13","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/BF01852390","article-title":"Comparative study with various anesthetics in mice (pentobarbitone, ketamine-xylazine, carfentanyl-etomidate","volume":"184","author":"Erhardt","year":"1984","journal-title":"Res Exp Med"},{"key":"10.1038\/labinvest.2009.83_bib14","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1083\/jcb.17.1.208","article-title":"The use of lead citrate at high pH as an electron-opaque stain in electron microscopy","volume":"17","author":"Reynolds","year":"1638","journal-title":"J Cell Biol"},{"key":"10.1038\/labinvest.2009.83_bib15","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1038\/nprot.2008.215","article-title":"A methodology for the combined in situ analyses of the precursor and mature forms of microRNAs and correlation with their putative targets","volume":"4","author":"Nuovo","year":"2009","journal-title":"Nat Protoc"},{"key":"10.1038\/labinvest.2009.83_bib16","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.1099\/0022-1317-81-9-2177","article-title":"Manifestation of thrombocytopenia in dengue-2-virus-infected mice","volume":"81","author":"Huang","year":"2000","journal-title":"J Gen Virol"},{"key":"10.1038\/labinvest.2009.83_bib17","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1111\/j.1574-695X.2003.tb00646.x","article-title":"Anti-TNF antibody treatment reduces mortality in experimental dengue virus infection","volume":"35","author":"Atrasheuskaya","year":"2003","journal-title":"FEMS Immunol Med Microbiol"},{"key":"10.1038\/labinvest.2009.83_bib18","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/0035-9203(81)90142-5","article-title":"Infection of young adult mice with dengue virus type 2","volume":"75","author":"Boonpucknavig","year":"1981","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"10.1038\/labinvest.2009.83_bib19","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1099\/0022-1317-52-1-71","article-title":"Inoculation of dengue virus into nude mice","volume":"52","author":"Hotta","year":"1981","journal-title":"J Gen Viral"},{"key":"10.1038\/labinvest.2009.83_bib20","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1006\/viro.1999.9887","article-title":"Development of anovel mouse model for dengue virus infection","volume":"263","author":"An","year":"1999","journal-title":"Virology"},{"key":"10.1038\/labinvest.2009.83_bib21","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.imlet.2004.07.006","article-title":"Dengue-specific CD8+ T cells have both protective and pathogenic roles in dengue virus infection","volume":"95","author":"An","year":"2004","journal-title":"Immunol Lett"},{"key":"10.1038\/labinvest.2009.83_bib22","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.virol.2003.09.048","article-title":"Early activation of natural killer and B cells in response to primary dengue virus infection in A\/J mice","volume":"319","author":"Shresta","year":"2004","journal-title":"Virology"},{"key":"10.1038\/labinvest.2009.83_bib23","doi-asserted-by":"crossref","first-page":"1808","DOI":"10.1086\/518007","article-title":"Dengue virus infects macrophages and dendritic cells in a mouse model of infection","volume":"195","author":"Kyle","year":"2007","journal-title":"J Infect Dis"},{"key":"10.1038\/labinvest.2009.83_bib24","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1099\/0022-1317-72-4-859","article-title":"Breakdown of the blood-brain barrier during dengue virus infection of mouse","volume":"72","author":"Chaturvedi","year":"1991","journal-title":"J Gen Virol"},{"key":"10.1038\/labinvest.2009.83_bib25","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1002\/jmv.20108","article-title":"Lymphocyte activation and hepaticcellular infiltration in immunocompetent mice infected by dengue virus","volume":"73","author":"Chen","year":"2004","journal-title":"J Med Virol"},{"key":"10.1038\/labinvest.2009.83_bib26","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1080\/00034983.1967.11686519","article-title":"Pathology of Thailand haemorrhagic fever: a study of 100 autopsy cases","volume":"61","author":"Bhamarapravati","year":"1967","journal-title":"Ann Trop Med Parasitol"},{"key":"10.1038\/labinvest.2009.83_bib27","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1016\/0035-9203(68)90120-X","article-title":"Dengue haemorrhagic fever: a pathological study","volume":"62","author":"Burke","year":"1968","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"10.1038\/labinvest.2009.83_bib28","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1086\/383043","article-title":"Localization of dengue virus in naturally infected human tissues, by immunohistochemistry and in situ hybridization","volume":"189","author":"Jessie","year":"2004","journal-title":"J Infect Dis"},{"key":"10.1038\/labinvest.2009.83_bib29","first-page":"451","article-title":"Philippine hemorrhagic fever: a clinical, laboratory, and necropsy study","volume":"73","author":"Fresh","year":"1969","journal-title":"J Lab Clin Med"},{"key":"10.1038\/labinvest.2009.83_bib30","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.actatropica.2005.03.003","article-title":"Reconsideration of histopathology and ultrastructural aspects of the human liver in yellow fever","volume":"94","author":"Quaresma","year":"2005","journal-title":"Acta Trop"},{"key":"10.1038\/labinvest.2009.83_bib31","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1002\/hep.20750","article-title":"Steatosis: Co-factor in other liver diseases","volume":"42","author":"Powell","year":"2005","journal-title":"Hepatology"},{"key":"10.1038\/labinvest.2009.83_bib32","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1002\/ajh.2830380420","article-title":"Dengue virus infection-associated hemophagocytic syndrome","volume":"38","author":"Wong","year":"1991","journal-title":"Am J Hematol"},{"key":"10.1038\/labinvest.2009.83_bib33","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/S1607-551X(09)70274-8","article-title":"Dengue virus-associated hemophagocytic syndrome and dyserythropoiesis: a case report","volume":"21","author":"Lu","year":"2005","journal-title":"Kaohsiung J Med Sci"},{"key":"10.1038\/labinvest.2009.83_bib34","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1179\/102453308X316095","article-title":"Dengue virus related hemophagocytosis: a rare case report","volume":"13","author":"Jain","year":"2008","journal-title":"Hematology"},{"key":"10.1038\/labinvest.2009.83_bib35","first-page":"104","article-title":"Hemophagocytic syndrome in Dengue hemorrhagic fever with severe multiorgan complications","volume":"91","author":"Srichaikul","year":"2008","journal-title":"J Med Assoc Thai"},{"key":"10.1038\/labinvest.2009.83_bib36","doi-asserted-by":"crossref","first-page":"265","DOI":"10.4269\/ajtmh.1992.47.265","article-title":"Liver biochemical tests and dengue fever","volume":"47","author":"Kuo","year":"1992","journal-title":"Am J Trop Med Hyg"},{"key":"10.1038\/labinvest.2009.83_bib37","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S0923-2516(97)88364-1","article-title":"The impact of dengue haemorrhagic fever on liver function","volume":"148","author":"Nguyen","year":"1997","journal-title":"Res Virol"},{"key":"10.1038\/labinvest.2009.83_bib38","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.trstmh.2006.02.019","article-title":"Hepatocyte lesions and cellular immune response in yellow fever infection","volume":"101","author":"Quaresma","year":"2007","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"10.1038\/labinvest.2009.83_bib39","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1590\/S1413-86702007000400007","article-title":"The impact of dengue on liver function as evaluated by aminotransferase levels","volume":"11","author":"Souza","year":"2007","journal-title":"Braz J Infect Dis"},{"key":"10.1038\/labinvest.2009.83_bib40","doi-asserted-by":"crossref","first-page":"1270","DOI":"10.1086\/314466","article-title":"Differing infection patterns of dengue and yellow fever viruses in a human hepatoma cell line","volume":"178","author":"Marianneau","year":"1998","journal-title":"J Infect Dis"},{"key":"10.1038\/labinvest.2009.83_bib41","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.jcv.2007.04.024","article-title":"Apoptosis in tissues from fatal dengue shock syndrome","volume":"40","author":"Limonta","year":"2007","journal-title":"J Clin Virol"},{"key":"10.1038\/labinvest.2009.83_bib42","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1016\/j.bbadis.2007.08.003","article-title":"Mitochondrial and bioenergetic dysfunction in human hepatic cells infected with dengue 2 virus","volume":"72","author":"El-Bacha","year":"2007","journal-title":"Biochim Biophys Acta"},{"key":"10.1038\/labinvest.2009.83_bib43","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1093\/tropej\/46.1.40","article-title":"Hepatic dysfunction in childhood dengue infection","volume":"46","author":"Mohan","year":"2000","journal-title":"J Trop Pediatr"},{"key":"10.1038\/labinvest.2009.83_bib44","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.jcv.2006.12.011","article-title":"Fulminant hepatitis in dengue haemorrhagic fever","volume":"38","author":"Ling","year":"2007","journal-title":"J Clin Virol"},{"key":"10.1038\/labinvest.2009.83_bib45","first-page":"204","article-title":"Retrospective study on dengue fatal cases","volume":"16","author":"Miagostovich","year":"1997","journal-title":"Clin Neuropathol"},{"key":"10.1038\/labinvest.2009.83_bib46","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.vaccine.2005.07.059","article-title":"Protection against dengue type v\u00edrus induced in mice immunized with a DNA plasmid encoding the non-structural 1 (NS1) gene fused to the tissue plasminogen activator signal sequence","volume":"24","author":"Costa","year":"2006","journal-title":"Vaccine"}],"container-title":["Laboratory Investigation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/labinvest200983.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/labinvest200983","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0023683722029555?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0023683722029555?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/labinvest200983.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T19:53:36Z","timestamp":1758398016000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0023683722029555"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,10]]},"references-count":46,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2009,10]]}},"alternative-id":["S0023683722029555"],"URL":"https:\/\/doi.org\/10.1038\/labinvest.2009.83","relation":{},"ISSN":["0023-6837"],"issn-type":[{"value":"0023-6837","type":"print"}],"subject":[],"published":{"date-parts":[[2009,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Hepatic damage associated with dengue-2 virus replication in liver cells of BALB\/c mice","name":"articletitle","label":"Article Title"},{"value":"Laboratory Investigation","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1038\/labinvest.2009.83","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2009 United States & Canadian Academy of Pathology.","name":"copyright","label":"Copyright"}]}}