{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T03:04:38Z","timestamp":1792206278508,"version":"4.4.0"},"reference-count":21,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T00:00:00Z","timestamp":1673395200000},"content-version":"vor","delay-in-days":4059,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["li.uscap.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Laboratory Investigation"],"published-print":{"date-parts":[[2011,12]]},"DOI":"10.1038\/labinvest.2011.94","type":"journal-article","created":{"date-parts":[[2011,9,19]],"date-time":"2011-09-19T13:21:02Z","timestamp":1316438462000},"page":"1787-1795","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"title":["Microfluidics-based capture of human neutrophils for expression analysis in blood and bronchoalveolar lavage"],"prefix":"10.1016","volume":"91","author":[{"given":"Elizabeth A","family":"Warner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth T","family":"Kotz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo F","family":"Ungaro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amer S","family":"Abouhamze","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M Cecilia","family":"Lopez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex G","family":"Cuenca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kindra M","family":"Kelly-Scumpia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Moreno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerri A","family":"O'Malley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer D","family":"Lanz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry V","family":"Baker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larry C","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet","family":"Toner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald G","family":"Tompkins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip A","family":"Efron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lyle L","family":"Moldawer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1038\/labinvest.2011.94_bib1","doi-asserted-by":"crossref","first-page":"220","DOI":"10.2119\/molmed.2009.00027","article-title":"A genomic score prognostic of outcome in trauma patients","volume":"15","author":"Warren","year":"2009","journal-title":"Mol Med (Cambridge, Mass)"},{"key":"10.1038\/labinvest.2011.94_bib2","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1097\/SLA.0b013e3181b8fbd5","article-title":"Validation of the riboleukogram to detect ventilator-associated pneumonia after severe injury","volume":"250","author":"Cobb","year":"2009","journal-title":"Ann Surg"},{"key":"10.1038\/labinvest.2011.94_bib3","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1038\/35000501","article-title":"Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling","volume":"403","author":"Alizadeh","year":"2000","journal-title":"Nature"},{"key":"10.1038\/labinvest.2011.94_bib4","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/S0140-6736(03)14023-8","article-title":"Gene expression profiling for the prediction of therapeutic response to docetaxel in patients with breast cancer","volume":"362","author":"Chang","year":"2003","journal-title":"Lancet"},{"key":"10.1038\/labinvest.2011.94_bib5","doi-asserted-by":"crossref","first-page":"2284","DOI":"10.1200\/JCO.2004.05.166","article-title":"Gene expression profiles predict complete pathologic response to neoadjuvant paclitaxel and fluorouracil, doxorubicin, and cyclophosphamide chemotherapy in breast cancer","volume":"22","author":"Ayers","year":"2004","journal-title":"J Clin Oncol"},{"key":"10.1038\/labinvest.2011.94_bib6","doi-asserted-by":"crossref","first-page":"8244","DOI":"10.1158\/1078-0432.CCR-08-0939","article-title":"Molecular grading of ductal carcinoma in situ of the breast","volume":"14","author":"Balleine","year":"2008","journal-title":"Clin Cancer Res"},{"key":"10.1038\/labinvest.2011.94_bib7","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/j.jamcollsurg.2006.06.028","article-title":"Molecular diagnostics in sepsis: from bedside to bench","volume":"203","author":"Chung","year":"2006","journal-title":"J Am Coll Surg"},{"key":"10.1038\/labinvest.2011.94_bib8","doi-asserted-by":"crossref","first-page":"7103","DOI":"10.4049\/jimmunol.172.11.7103","article-title":"Genomic and proteomic determinants of outcome in patients undergoing thoracoabdominal aortic aneurysm repair","volume":"172","author":"Feezor","year":"2004","journal-title":"J Immunol"},{"key":"10.1038\/labinvest.2011.94_bib9","doi-asserted-by":"crossref","first-page":"2066","DOI":"10.1182\/blood-2006-02-002477","article-title":"Gene expression patterns in blood leukocytes discriminate patients with acute infections","volume":"109","author":"Ramilo","year":"2007","journal-title":"Blood"},{"key":"10.1038\/labinvest.2011.94_bib10","doi-asserted-by":"crossref","first-page":"15564","DOI":"10.1073\/pnas.0607028103","article-title":"Cell-specific expression and pathway analyses reveal alterations in trauma-related human T ell and monocyte pathways","volume":"103","author":"Laudanski","year":"2006","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/labinvest.2011.94_bib11","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1038\/nature03985","article-title":"A network-based analysis of systemic inflammation in humans","volume":"437","author":"Calvano","year":"2005","journal-title":"Nature"},{"key":"10.1038\/labinvest.2011.94_bib12","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1097\/01.shk.0000163394.93467.77","article-title":"Genome-wide monocytic mRNA expression in polytrauma patients for identification of clinical outcome","volume":"24","author":"Biberthaler","year":"2005","journal-title":"Shock (Augusta, Ga)"},{"key":"10.1038\/labinvest.2011.94_bib13","doi-asserted-by":"crossref","first-page":"4801","DOI":"10.1073\/pnas.0409768102","article-title":"Application of genome-wide expression analysis to human health and disease","volume":"102","author":"Cobb","year":"2005","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/labinvest.2011.94_bib14","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/BF02351217","article-title":"Neutrophil function in whole blood and after purification: changes in receptor expression, oxidase activity and responsiveness to cytokines","volume":"12","author":"Watson","year":"1992","journal-title":"Biosci Rep"},{"key":"10.1038\/labinvest.2011.94_bib15","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0022-1759(89)90425-0","article-title":"Rapid method for the isolation of neutrophils in high yield without the use of dextran or density gradient polymers","volume":"121","author":"Eggleton","year":"1989","journal-title":"J Immunol Methods"},{"key":"10.1038\/labinvest.2011.94_bib16","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/0022-1759(80)90115-5","article-title":"Separation of granulocytes from peripheral blood in a single step using discontinuous density gradients of Ficoll-Urografin. A comparative study with separation by dextran","volume":"32","author":"Aguado","year":"1980","journal-title":"J Immunol Methods"},{"key":"10.1038\/labinvest.2011.94_bib17","series-title":"Methods in Molecular Biology","author":"Nauseef","year":"2007"},{"key":"10.1038\/labinvest.2011.94_bib18","doi-asserted-by":"crossref","first-page":"5453","DOI":"10.1021\/ac060140c","article-title":"Microfluidic isolation of leukocytes from whole blood for phenotype and gene expression analysis","volume":"78","author":"Sethu","year":"2006","journal-title":"Anal Chem"},{"key":"10.1038\/labinvest.2011.94_bib19","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1373\/clinchem.2007.099150","article-title":"Microfluidic leukocyte isolation for gene expression analysis in critically ill hospitalized patients","volume":"54","author":"Russom","year":"2008","journal-title":"Clin Chem"},{"key":"10.1038\/labinvest.2011.94_bib20","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1038\/nm.2205","article-title":"Clinical microfluidics for neutrophil genomics and proteomics","volume":"16","author":"Kotz","year":"2010","journal-title":"Nat Med"},{"key":"10.1038\/labinvest.2011.94_bib21","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1038\/nrg1293","article-title":"Maximizing the potential of functional genomics","volume":"5","author":"Steinmetz","year":"2004","journal-title":"Nat Rev"}],"container-title":["Laboratory Investigation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/labinvest201194.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/labinvest201194","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002368372203241X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002368372203241X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/labinvest201194.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T19:55:58Z","timestamp":1758398158000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S002368372203241X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12]]},"references-count":21,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2011,12]]}},"alternative-id":["S002368372203241X"],"URL":"https:\/\/doi.org\/10.1038\/labinvest.2011.94","relation":{},"ISSN":["0023-6837"],"issn-type":[{"value":"0023-6837","type":"print"}],"subject":[],"published":{"date-parts":[[2011,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Microfluidics-based capture of human neutrophils for expression analysis in blood and bronchoalveolar lavage","name":"articletitle","label":"Article Title"},{"value":"Laboratory Investigation","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1038\/labinvest.2011.94","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2011 United States & Canadian Academy of Pathology.","name":"copyright","label":"Copyright"}]}}