{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T01:08:37Z","timestamp":1782781717498,"version":"3.54.5"},"reference-count":47,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T00:00:00Z","timestamp":1673395200000},"content-version":"vor","delay-in-days":1867,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["li.uscap.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Laboratory Investigation"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1038\/labinvest.2017.96","type":"journal-article","created":{"date-parts":[[2017,10,16]],"date-time":"2017-10-16T08:45:43Z","timestamp":1508143543000},"page":"1412-1426","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":27,"title":["Upregulation of the actin cytoskeleton via myocardin leads to increased expression of type 1 collagen"],"prefix":"10.1016","volume":"97","author":[{"given":"Zengdun","family":"Shi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Don C","family":"Rockey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1038\/labinvest.2017.96_bib1","doi-asserted-by":"crossref","first-page":"1622","DOI":"10.1016\/j.biocel.2010.04.007","article-title":"Actin cytoskeleton dynamics and the cell division cycle","volume":"42","author":"Heng","year":"2010","journal-title":"Int J Biochem Cell Biol"},{"key":"10.1038\/labinvest.2017.96_bib2","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1146\/annurev.cellbio.011209.122036","article-title":"Mechanical integration of actin and adhesion dynamics in cell migration","volume":"26","author":"Gardel","year":"2010","journal-title":"Annu Rev Cell Dev Biol"},{"key":"10.1038\/labinvest.2017.96_bib3","doi-asserted-by":"crossref","first-page":"e77166","DOI":"10.1371\/journal.pone.0077166","article-title":"Smooth muscle alpha actin (Acta2) and myofibroblast function during hepatic wound healing","volume":"8","author":"Rockey","year":"2013","journal-title":"PLoS One"},{"key":"10.1038\/labinvest.2017.96_bib4","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1161\/ATVBAHA.110.221135","article-title":"Signaling mechanisms that regulate smooth muscle cell differentiation","volume":"31","author":"Mack","year":"2011","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"10.1038\/labinvest.2017.96_bib5","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.trsl.2011.05.004","article-title":"Actin cytoskeleton in myofibroblast differentiation: ultrastructure defining form and driving function","volume":"158","author":"Sandbo","year":"2011","journal-title":"Transl Res"},{"key":"10.1038\/labinvest.2017.96_bib6","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.bbamcr.2006.07.001","article-title":"Regulation of the actin cytoskeleton in cancer cell migration and invasion","volume":"1773","author":"Yamaguchi","year":"2007","journal-title":"Biochim Biophys Acta"},{"key":"10.1038\/labinvest.2017.96_bib7","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1038\/nrm3758","article-title":"Molecular mechanisms of epithelial-mesenchymal transition","volume":"15","author":"Lamouille","year":"2014","journal-title":"Nat Rev Mol Cell Biol"},{"key":"10.1038\/labinvest.2017.96_bib8","doi-asserted-by":"crossref","first-page":"1807","DOI":"10.2353\/ajpath.2007.070112","article-title":"The myofibroblast: one function, multiple origins","volume":"170","author":"Hinz","year":"2007","journal-title":"Am J Pathol"},{"key":"10.1038\/labinvest.2017.96_bib9","doi-asserted-by":"crossref","first-page":"S17","DOI":"10.1186\/1755-1536-5-S1-S17","article-title":"Origin of myofibroblasts in liver fibrosis","volume":"5","author":"Brenner","year":"2012","journal-title":"Fibrogenesis Tissue Repair"},{"key":"10.1038\/labinvest.2017.96_bib10","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1247\/csf.28.105","article-title":"Hepatic stellate cells: unique characteristics in cell biology and phenotype","volume":"28","author":"Sato","year":"2003","journal-title":"Cell Struct Funct"},{"key":"10.1038\/labinvest.2017.96_bib11","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1002\/cm.20475","article-title":"The actin gene family: function follows isoform","volume":"67","author":"Perrin","year":"2010","journal-title":"Cytoskeleton (Hoboken)"},{"key":"10.1038\/labinvest.2017.96_bib12","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1615\/CritRevEukarGeneExpr.v21.i3.30","article-title":"Structural and functional properties of the actin gene family","volume":"21","author":"Bunnell","year":"2011","journal-title":"Crit Rev Eukaryot Gene Expr"},{"key":"10.1038\/labinvest.2017.96_bib13","doi-asserted-by":"crossref","first-page":"4047","DOI":"10.1091\/mbc.e11-06-0582","article-title":"beta-Actin specifically controls cell growth, migration, and the G-actin pool","volume":"22","author":"Bunnell","year":"2011","journal-title":"Mol Biol Cell"},{"key":"10.1038\/labinvest.2017.96_bib14","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1002\/cm.20467","article-title":"Delayed embryonic development and impaired cell growth and survival in Actg1 null mice","volume":"67","author":"Bunnell","year":"2010","journal-title":"Cytoskeleton (Hoboken)"},{"key":"10.1038\/labinvest.2017.96_bib15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ydbio.2011.11.002","article-title":"Smooth muscle alpha actin is specifically required for the maintenance of lactation","volume":"363","author":"Weymouth","year":"2012","journal-title":"Dev Biol"},{"key":"10.1038\/labinvest.2017.96_bib16","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1152\/physrev.00041.2003","article-title":"Molecular regulation of vascular smooth muscle cell differentiation in development and disease","volume":"84","author":"Owens","year":"2004","journal-title":"Physiol Rev"},{"key":"10.1038\/labinvest.2017.96_bib17","doi-asserted-by":"crossref","first-page":"C70","DOI":"10.1152\/ajpcell.00386.2006","article-title":"Serum response factor: master regulator of the actin cytoskeleton and contractile apparatus","volume":"292","author":"Miano","year":"2007","journal-title":"Am J Physiol Cell Physiol"},{"key":"10.1038\/labinvest.2017.96_bib18","doi-asserted-by":"crossref","first-page":"32415","DOI":"10.1074\/jbc.M110.164863","article-title":"Interferon-gamma-mediated inhibition of serum response factor-dependent smooth muscle-specific gene expression","volume":"285","author":"Shi","year":"2010","journal-title":"J Biol Chem"},{"key":"10.1038\/labinvest.2017.96_bib19","doi-asserted-by":"crossref","first-page":"6913","DOI":"10.1093\/nar\/19.24.6913","article-title":"Transcription factor binding and spacing constraints in the human beta-actin proximal promoter","volume":"19","author":"Danilition","year":"1991","journal-title":"Nucleic Acids Res"},{"key":"10.1038\/labinvest.2017.96_bib20","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1161\/01.RES.0000259563.61091.e8","article-title":"Myocardin-related transcription factors: critical coactivators regulating cardiovascular development and adaptation","volume":"100","author":"Parmacek","year":"2007","journal-title":"Circ Res"},{"key":"10.1038\/labinvest.2017.96_bib21","doi-asserted-by":"crossref","first-page":"4447","DOI":"10.1073\/pnas.1420363112","article-title":"Myocardin is required for maintenance of vascular and visceral smooth muscle homeostasis during postnatal development","volume":"112","author":"Huang","year":"2015","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/labinvest.2017.96_bib22","doi-asserted-by":"crossref","first-page":"9366","DOI":"10.1073\/pnas.1233635100","article-title":"The serum response factor coactivator myocardin is required for vascular smooth muscle development","volume":"100","author":"Li","year":"2003","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/labinvest.2017.96_bib23","doi-asserted-by":"crossref","first-page":"7129","DOI":"10.1073\/pnas.1232341100","article-title":"Myocardin is a master regulator of smooth muscle gene expression","volume":"100","author":"Wang","year":"2003","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/labinvest.2017.96_bib24","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1111\/j.1478-3231.2009.02120.x","article-title":"Gene expression profiling and functional assays of activated hepatic stellate cells suggest that myocardin has a role in activation","volume":"30","author":"Shimada","year":"2010","journal-title":"Liver Int"},{"key":"10.1038\/labinvest.2017.96_bib25","first-page":"193","article-title":"Rat hepatic lipocytes express smooth muscle actin upon activation in vivo and in culture","volume":"24","author":"Rockey","year":"1992","journal-title":"J Submicrosc Cytol Pathol"},{"key":"10.1038\/labinvest.2017.96_bib26","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1038\/sj.gt.3300664","article-title":"Enhanced in vivo adenovirus-mediated gene transfer to rat hepatocarcinomas by selective administration into the hepatic artery","volume":"5","author":"Gerolami","year":"1998","journal-title":"Gene Ther"},{"key":"10.1038\/labinvest.2017.96_bib27","doi-asserted-by":"crossref","first-page":"G565","DOI":"10.1152\/ajpgi.00512.2000","article-title":"Adenovirus-mediated gene transfer to nonparenchymal cells in normal and injured liver","volume":"282","author":"Yu","year":"2002","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"10.1038\/labinvest.2017.96_bib28","first-page":"14","article-title":"The mitochondria-targeted antioxidant MitoQ attenuates liver fibrosis in mice","volume":"8","author":"Rehman","year":"2016","journal-title":"Int J Physiol Pathophysiol Pharmacol"},{"key":"10.1038\/labinvest.2017.96_bib29","doi-asserted-by":"crossref","first-page":"G948","DOI":"10.1152\/ajpgi.00359.2011","article-title":"Preproendothelin-1 expression is negatively regulated by IFNgamma during hepatic stellate cell activation","volume":"302","author":"Li","year":"2012","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"10.1038\/labinvest.2017.96_bib30","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1016\/S0092-8674(01)00404-4","article-title":"Activation of cardiac gene expression by myocardin, a transcriptional cofactor for serum response factor","volume":"105","author":"Wang","year":"2001","journal-title":"Cell"},{"key":"10.1038\/labinvest.2017.96_bib31","doi-asserted-by":"crossref","first-page":"1976","DOI":"10.1111\/j.1538-7836.2005.01316.x","article-title":"Regulation of smooth muscle differentiation by the myocardin family of serum response factor co-factors","volume":"3","author":"Mack","year":"2005","journal-title":"J Thromb Haemost"},{"key":"10.1038\/labinvest.2017.96_bib32","doi-asserted-by":"crossref","first-page":"2425","DOI":"10.1128\/MCB.23.7.2425-2437.2003","article-title":"Myocardin is a critical serum response factor cofactor in the transcriptional program regulating smooth muscle cell differentiation","volume":"23","author":"Du","year":"2003","journal-title":"Mol Cell Biol"},{"key":"10.1038\/labinvest.2017.96_bib33","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1161\/01.RES.0000125622.46280.95","article-title":"Myocardin and Prx1 contribute to angiotensin II-induced expression of smooth muscle alpha-actin","volume":"94","author":"Yoshida","year":"2004","journal-title":"Circ Res"},{"key":"10.1038\/labinvest.2017.96_bib34","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.jhep.2014.07.029","article-title":"Myocardin related transcription factor A programs epigenetic activation of hepatic stellate cells","volume":"62","author":"Tian","year":"2015","journal-title":"J Hepatol"},{"key":"10.1038\/labinvest.2017.96_bib35","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1101\/gad.1428006","article-title":"The myocardin family of transcriptional coactivators: versatile regulators of cell growth, migration, and myogenesis","volume":"20","author":"Pipes","year":"2006","journal-title":"Genes Dev"},{"key":"10.1038\/labinvest.2017.96_bib36","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1038\/nrm2890","article-title":"Linking actin dynamics and gene transcription to drive cellular motile functions","volume":"11","author":"Olson","year":"2010","journal-title":"Nat Rev Mol Cell Biol"},{"key":"10.1038\/labinvest.2017.96_bib37","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.yjmcc.2013.10.019","article-title":"Myofibroblasts: trust your heart and let fate decide","volume":"70","author":"Davis","year":"2014","journal-title":"J Mol Cell Cardiol"},{"key":"10.1038\/labinvest.2017.96_bib38","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1016\/S0168-8278(01)00169-6","article-title":"Inhibitory effect of Y-27632, a ROCK inhibitor, on progression of rat liver fibrosis in association with inactivation of hepatic stellate cells","volume":"35","author":"Murata","year":"2001","journal-title":"J Hepatol"},{"key":"10.1038\/labinvest.2017.96_bib39","doi-asserted-by":"crossref","first-page":"18222","DOI":"10.1074\/jbc.272.29.18222","article-title":"Organization and myogenic restricted expression of the murine serum response factor gene. A role for autoregulation","volume":"272","author":"Belaguli","year":"1997","journal-title":"J Biol Chem"},{"key":"10.1038\/labinvest.2017.96_bib40","doi-asserted-by":"crossref","first-page":"44116","DOI":"10.1074\/jbc.M111.276931","article-title":"Myocardin-related transcription factor-A complexes activate type I collagen expression in lung fibroblasts","volume":"286","author":"Luchsinger","year":"2011","journal-title":"J Biol Chem"},{"key":"10.1038\/labinvest.2017.96_bib41","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1111\/j.1432-0436.2007.00260.x","article-title":"Hepatic stellate cells display a functional vascular smooth muscle cell phenotype in a three-dimensional co-culture model with endothelial cells","volume":"76","author":"Wirz","year":"2008","journal-title":"Differentiation"},{"key":"10.1038\/labinvest.2017.96_bib42","doi-asserted-by":"crossref","first-page":"L824","DOI":"10.1152\/ajplung.00350.2015","article-title":"alpha-Smooth muscle actin is an inconsistent marker of fibroblasts responsible for force-dependent TGFbeta activation or collagen production across multiple models of organ fibrosis","volume":"310","author":"Sun","year":"2016","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"10.1038\/labinvest.2017.96_bib43","doi-asserted-by":"crossref","first-page":"40193","DOI":"10.1074\/jbc.M602182200","article-title":"Smooth muscle alpha-actin deficiency in myofibroblasts leads to enhanced renal tissue fibrosis","volume":"281","author":"Takeji","year":"2006","journal-title":"J Biol Chem"},{"key":"10.1038\/labinvest.2017.96_bib44","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1172\/JCI22648","article-title":"5\u2018 CArG degeneracy in smooth muscle alpha-actin is required for injury-induced gene suppression in vivo","volume":"115","author":"Hendrix","year":"2005","journal-title":"J Clin Invest"},{"key":"10.1038\/labinvest.2017.96_bib45","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1096\/fj.03-1273rev","article-title":"TGF-beta signaling and the fibrotic response","volume":"18","author":"Leask","year":"2004","journal-title":"FASEB J"},{"key":"10.1038\/labinvest.2017.96_bib46","doi-asserted-by":"crossref","first-page":"16850","DOI":"10.1073\/pnas.1316764110","article-title":"Activation of MRTF-A-dependent gene expression with a small molecule promotes myofibroblast differentiation and wound healing","volume":"110","author":"Velasquez","year":"2013","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/labinvest.2017.96_bib47","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1161\/CIRCRESAHA.110.223172","article-title":"Myocardin-related transcription factor-a controls myofibroblast activation and fibrosis in response to myocardial infarction","volume":"107","author":"Small","year":"2010","journal-title":"Circ Res"}],"container-title":["Laboratory Investigation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/labinvest201796.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/labinvest201796","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0023683722007450?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0023683722007450?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/doifinder\/10.1038\/labinvest.2017.96","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/labinvest201796.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T19:30:54Z","timestamp":1758396654000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0023683722007450"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":47,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["S0023683722007450"],"URL":"https:\/\/doi.org\/10.1038\/labinvest.2017.96","relation":{},"ISSN":["0023-6837"],"issn-type":[{"value":"0023-6837","type":"print"}],"subject":[],"published":{"date-parts":[[2017,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Upregulation of the actin cytoskeleton via myocardin leads to increased expression of type 1 collagen","name":"articletitle","label":"Article Title"},{"value":"Laboratory Investigation","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1038\/labinvest.2017.96","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 United States & Canadian Academy of Pathology.","name":"copyright","label":"Copyright"}]}}