{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T07:34:24Z","timestamp":1789803264708,"version":"4.0.1"},"reference-count":56,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T00:00:00Z","timestamp":1454284800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T00:00:00Z","timestamp":1454284800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T00:00:00Z","timestamp":1673395200000},"content-version":"vor","delay-in-days":2536,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["mp.uscap.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Modern Pathology"],"published-print":{"date-parts":[[2016,2]]},"DOI":"10.1038\/modpathol.2015.144","type":"journal-article","created":{"date-parts":[[2016,1,15]],"date-time":"2016-01-15T14:13:34Z","timestamp":1452867214000},"page":"182-193","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":51,"title":["High-definition CpG methylation of novel genes in gastric carcinogenesis identified by next-generation sequencing"],"prefix":"10.1016","volume":"29","author":[{"given":"Jorge L","family":"Sepulveda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge L","family":"Gutierrez-Pajares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aesis","family":"Luna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W","family":"Tobias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanghee","family":"Woo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Giorgi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena V","family":"Komissarova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Califano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy C","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia R","family":"Sepulveda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1038\/modpathol.2015.144_bib1","doi-asserted-by":"crossref","first-page":"2137","DOI":"10.1200\/JCO.2005.05.2308","article-title":"Patterns of cancer incidence, mortality, and prevalence across five continents: defining priorities to reduce cancer disparities in different geographic regions of the world","volume":"24","author":"Kamangar","year":"2006","journal-title":"J Clin Oncol"},{"key":"10.1038\/modpathol.2015.144_bib2","first-page":"74","article-title":"Global cancer statistics, 2002","volume":"55","author":"Parkin","year":"2005","journal-title":"CA Cancer J Clin"},{"key":"10.1038\/modpathol.2015.144_bib3","unstructured":"International Agency for Research on Cancer. GLOBOCAN 2012: Estimated Cancer Incidence, Mortality and Prevalence Worldwide in 2012 http:\/\/globocan.iarc.fr\/Pages\/fact_sheets_cancer.aspx 2012, Accessed on: 13 January 2015."},{"key":"10.1038\/modpathol.2015.144_bib4","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1001\/jama.2014.10498","article-title":"Prevention of gastric cancer","volume":"312","author":"Herrero","year":"2014","journal-title":"JAMA"},{"key":"10.1038\/modpathol.2015.144_bib5","unstructured":"American Cancer Society. What are the key statistics about stomach cancer? http:\/\/www.cancer.org\/acs\/groups\/content\/@nho\/documents\/document\/globalfactsandfigures2007rev2p.pdf 2012, Accessed on: 8 February 2015."},{"key":"10.1038\/modpathol.2015.144_bib6","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.gtc.2013.01.002","article-title":"Gastric cancer: overview","volume":"42","author":"Correa","year":"2013","journal-title":"Gastroenterol Clin North Am"},{"key":"10.1038\/modpathol.2015.144_bib7","doi-asserted-by":"crossref","first-page":"2282","DOI":"10.1016\/j.cgh.2015.05.040","article-title":"Cancer Risk after pernicious anemia in the US elderly population","volume":"13","author":"Murphy","year":"2015","journal-title":"Clin Gastroenterol Hepatol"},{"key":"10.1038\/modpathol.2015.144_bib8","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1111\/j.1469-0691.2009.03031.x","article-title":"Helicobacter pylori and gastric adenocarcinoma","volume":"15","author":"Herrera","year":"2009","journal-title":"Clin Microbiol Infect"},{"key":"10.1038\/modpathol.2015.144_bib9","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/S0889-8553(02)00012-2","article-title":"Role of Helicobacter pylori in gastric carcinogenesis","volume":"31","author":"Sepulveda","year":"2002","journal-title":"Gastroenterol Clin North Am"},{"key":"10.1038\/modpathol.2015.144_bib10","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/S0002-9440(10)64758-X","article-title":"Microsatellite instability in gastric intestinal metaplasia in patients with and without gastric cancer","volume":"156","author":"Leung","year":"2000","journal-title":"Am J Pathol"},{"key":"10.1038\/modpathol.2015.144_bib11","doi-asserted-by":"crossref","first-page":"8681","DOI":"10.1073\/pnas.96.15.8681","article-title":"CpG island methylator phenotype in colorectal cancer","volume":"96","author":"Toyota","year":"1999","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/modpathol.2015.144_bib12","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1002\/ijc.10783","article-title":"Promoter hypermethylation of tumor-related genes in gastric intestinal metaplasia of patients with and without gastric cancer","volume":"102","author":"To","year":"2002","journal-title":"Int J Cancer"},{"key":"10.1038\/modpathol.2015.144_bib13","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1097\/01.LAB.0000067481.08984.3F","article-title":"Profile of aberrant CpG island methylation along the multistep pathway of gastric carcinogenesis","volume":"83","author":"Kang","year":"2003","journal-title":"Lab Invest"},{"key":"10.1038\/modpathol.2015.144_bib14","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.cll.2004.12.002","article-title":"Molecular markers in Helicobacter pylori-associated gastric carcinogenesis","volume":"25","author":"Gologan","year":"2005","journal-title":"Clin Lab Med"},{"key":"10.1038\/modpathol.2015.144_bib15","doi-asserted-by":"crossref","first-page":"266","DOI":"10.2353\/jmoldx.2009.080125","article-title":"CpG methylation analysis\u2014current status of clinical assays and potential applications in molecular diagnostics: a report of the Association for Molecular Pathology","volume":"11","author":"Sepulveda","year":"2009","journal-title":"J Mol Diagn"},{"key":"10.1038\/modpathol.2015.144_bib16","doi-asserted-by":"crossref","first-page":"2367","DOI":"10.1002\/ijc.24219","article-title":"DNA methylation of microRNA genes in gastric mucosae of gastric cancer patients: its possible involvement in the formation of epigenetic field defect","volume":"124","author":"Ando","year":"2009","journal-title":"Int J Cancer"},{"key":"10.1038\/modpathol.2015.144_bib17","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1038\/nature13480","article-title":"Comprehensive molecular characterization of gastric adenocarcinoma","volume":"513","author":"TCGA","year":"2014","journal-title":"Nature"},{"key":"10.1038\/modpathol.2015.144_bib18","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1186\/1471-230X-14-55","article-title":"DNA methylation subgroups and the CpG island methylator phenotype in gastric cancer: a comprehensive profiling approach","volume":"14","author":"Loh","year":"2014","journal-title":"BMC Gastroenterol"},{"key":"10.1038\/modpathol.2015.144_bib19","doi-asserted-by":"crossref","first-page":"1836","DOI":"10.1053\/j.gastro.2009.12.042","article-title":"CpG methylation and reduced expression of O6-methylguanine DNA methyltransferase is associated with Helicobacter pylori infection","volume":"138","author":"Sepulveda","year":"2010","journal-title":"Gastroenterology"},{"key":"10.1038\/modpathol.2015.144_bib20","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1136\/gut.2005.077776","article-title":"Eradication of Helicobacter pylori infection reverses E-cadherin promoter hypermethylation","volume":"55","author":"Chan","year":"2006","journal-title":"Gut"},{"key":"10.1038\/modpathol.2015.144_bib21","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1016\/S0002-9440(10)63511-0","article-title":"Aberrant CpG island hypermethylation of chronic gastritis, in relation to aging, gender, intestinal metaplasia, and chronic inflammation","volume":"163","author":"Kang","year":"2003","journal-title":"Am J Pathol"},{"key":"10.1038\/modpathol.2015.144_bib22","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1038\/labinvest.3700117","article-title":"Alterations of DNA mismatch repair proteins and microsatellite instability levels in gastric cancer cell lines","volume":"84","author":"Yao","year":"2004","journal-title":"Lab Invest"},{"key":"10.1038\/modpathol.2015.144_bib23","doi-asserted-by":"crossref","first-page":"555","DOI":"10.3892\/ol_00000098","article-title":"Molecular identification of Helicobacter DNA in human gastric adenocarcinoma tissues using Helicobacter species-specific 16S rRNA PCR amplification and pyrosequencing analysis","volume":"1","author":"Han","year":"2010","journal-title":"Oncol Lett"},{"key":"10.1038\/modpathol.2015.144_bib24","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1101\/gr.4410706","article-title":"High-throughput DNA methylation profiling using universal bead arrays","volume":"16","author":"Bibikova","year":"2006","journal-title":"Genome Res"},{"key":"10.1038\/modpathol.2015.144_bib25","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1186\/1471-2105-11-587","article-title":"Comparison of Beta-value and M-value methods for quantifying methylation levels by microarray analysis","volume":"11","author":"Du","year":"2010","journal-title":"BMC Bioinform"},{"key":"10.1038\/modpathol.2015.144_bib26","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.1093\/bioinformatics\/18.11.1427","article-title":"MethPrimer: designing primers for methylation PCRs","volume":"18","author":"Li","year":"2002","journal-title":"Bioinformatics"},{"key":"10.1038\/modpathol.2015.144_bib27","unstructured":"Braham Bioinformatics. Trim Galore http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/trim_galore\/ 2014, Accessed on: 15 January 2015."},{"key":"10.1038\/modpathol.2015.144_bib28","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1093\/bioinformatics\/btr167","article-title":"Bismark: a flexible aligner and methylation caller for Bisulfite-Seq applications","volume":"27","author":"Krueger","year":"2011","journal-title":"Bioinformatics"},{"key":"10.1038\/modpathol.2015.144_bib29","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat Methods"},{"key":"10.1038\/modpathol.2015.144_bib30","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1038\/nbt.2203","article-title":"Absolute quantification of somatic DNA alterations in human cancer","volume":"30","author":"Carter","year":"2012","journal-title":"Nat Biotechnol"},{"key":"10.1038\/modpathol.2015.144_bib31","unstructured":"R Core Team. A language and environment for statistical computing http:\/\/www.R-project.org\/ 2015, Accessed on: 9 February 2015."},{"key":"10.1038\/modpathol.2015.144_bib32","doi-asserted-by":"crossref","first-page":"9440","DOI":"10.1073\/pnas.1530509100","article-title":"Statistical significance for genomewide studies","volume":"100","author":"Storey","year":"2003","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/modpathol.2015.144_bib33","doi-asserted-by":"crossref","first-page":"R106","DOI":"10.1186\/gb-2010-11-10-r106","article-title":"Differential expression analysis for sequence count data","volume":"11","author":"Anders","year":"2010","journal-title":"Genome Biol"},{"key":"10.1038\/modpathol.2015.144_bib34","first-page":"8606","article-title":"Epigenetic inactivation of CHFR and sensitivity to microtubule inhibitors in gastric cancer","volume":"63","author":"Satoh","year":"2003","journal-title":"Cancer Res"},{"key":"10.1038\/modpathol.2015.144_bib35","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1038\/labinvest.3700707","article-title":"DNA methylation profiles of gastric carcinoma characterized by quantitative DNA methylation analysis","volume":"88","author":"Kang","year":"2008","journal-title":"Lab Invest"},{"key":"10.1038\/modpathol.2015.144_bib36","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1016\/j.humpath.2007.01.030","article-title":"Differential expression of EphA7 receptor tyrosine kinase in gastric carcinoma","volume":"38","author":"Wang","year":"2007","journal-title":"Hum Pathol"},{"key":"10.1038\/modpathol.2015.144_bib37","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1007\/s12032-014-0907-6","article-title":"Association between CHFR methylation and chemosensitivity of paclitaxel in advanced gastric cancer","volume":"31","author":"Wang","year":"2014","journal-title":"Med Oncol"},{"key":"10.1038\/modpathol.2015.144_bib38","first-page":"49","article-title":"DNA methylation of CHFR is not a predictor of the response to docetaxel and paclitaxel in advanced and recurrent gastric cancer","volume":"26","author":"Yoshida","year":"2006","journal-title":"Anticancer Res"},{"key":"10.1038\/modpathol.2015.144_bib39","doi-asserted-by":"crossref","first-page":"2520","DOI":"10.3748\/wjg.15.2520","article-title":"Checkpoint with forkhead-associated and ring finger promoter hypermethylation correlates with microsatellite instability in gastric cancer","volume":"15","author":"Oki","year":"2009","journal-title":"World J Gastroenterol"},{"key":"10.1038\/modpathol.2015.144_bib40","first-page":"2714","article-title":"Deficiencies in Chfr and Mlh1 synergistically enhance tumor susceptibility in mice","volume":"119","author":"Fu","year":"2009","journal-title":"J Clin Invest"},{"key":"10.1038\/modpathol.2015.144_bib41","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.molbrainres.2004.04.001","article-title":"Identification and characterization of novel developmentally regulated neural-specific proteins, BRINP family","volume":"125","author":"Kawano","year":"2004","journal-title":"Brain Res Mol Brain Res"},{"key":"10.1038\/modpathol.2015.144_bib42","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1006\/geno.1997.5165","article-title":"Structure and methylation-based silencing of a gene (DBCCR1) within a candidate bladder cancer tumor suppressor region at 9q32-q33","volume":"48","author":"Habuchi","year":"1998","journal-title":"Genomics"},{"key":"10.1038\/modpathol.2015.144_bib43","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1038\/modpathol.2008.27","article-title":"Frequent hypermethylation of DBC1 in malignant lymphoproliferative neoplasms","volume":"21","author":"Gronbaek","year":"2008","journal-title":"Mod Pathol"},{"key":"10.1038\/modpathol.2015.144_bib44","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1093\/hmg\/ddi092","article-title":"Frequent silencing of DBC1 is by genetic or epigenetic mechanisms in non-small cell lung cancers","volume":"14","author":"Izumi","year":"2005","journal-title":"Hum Mol Genet"},{"key":"10.1038\/modpathol.2015.144_bib45","first-page":"335","article-title":"Low expression but infrequent genomic loss of the putative tumour suppressor DBCCR1 in astrocytoma","volume":"13","author":"Beetz","year":"2005","journal-title":"Oncol Rep"},{"key":"10.1038\/modpathol.2015.144_bib46","first-page":"1209","article-title":"MMP-7 and SGCE as distinctive molecular factors in sporadic colorectal cancers from the mutator phenotype pathway","volume":"36","author":"Ortega","year":"2010","journal-title":"Int J Oncol"},{"key":"10.1038\/modpathol.2015.144_bib47","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1161\/CIRCRESAHA.1134303145","article-title":"Fli1 acts downstream of Etv2 to govern cell survival and vascular homeostasis via positive autoregulation","volume":"114","author":"Abedin","year":"2014","journal-title":"Circ Res"},{"key":"10.1038\/modpathol.2015.144_bib48","doi-asserted-by":"crossref","first-page":"2855","DOI":"10.1002\/ijc.25951","article-title":"Identification and validation of highly frequent CpG island hypermethylation in colorectal adenomas and carcinomas","volume":"129","author":"Oster","year":"2011","journal-title":"Int J Cancer"},{"issue":"Suppl 3","key":"10.1038\/modpathol.2015.144_bib49","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1245\/s10434-014-4277-2","article-title":"Clinical relevance of plasma DNA methylation in colorectal cancer patients identified by using a genome-wide high-resolution array","volume":"22","author":"Lin","year":"2015","journal-title":"Ann Surg Oncol"},{"key":"10.1038\/modpathol.2015.144_bib50","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1016\/j.neo.2014.08.007","article-title":"FLI1 expression is correlated with breast cancer cellular growth, migration, and invasion and altered gene expression","volume":"16","author":"Scheiber","year":"2014","journal-title":"Neoplasia"},{"key":"10.1038\/modpathol.2015.144_bib51","doi-asserted-by":"crossref","first-page":"7604","DOI":"10.1158\/1078-0432.CCR-07-4673","article-title":"Epigenetic inactivation of galanin receptor 1 in head and neck cancer","volume":"14","author":"Misawa","year":"2008","journal-title":"Clin Cancer Res"},{"key":"10.1038\/modpathol.2015.144_bib52","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.leukres.2009.06.019","article-title":"CpG methylation analysis of the MEG3 and SNRPN imprinted genes in acute myeloid leukemia and myelodysplastic syndromes","volume":"34","author":"Benetatos","year":"2010","journal-title":"Leuk Res"},{"key":"10.1038\/modpathol.2015.144_bib53","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1002\/ijc.29449","article-title":"The benefit of microsatellite instability is attenuated by chemotherapy in stage II and stage III gastric cancer: Results from a large cohort with subgroup analyses","volume":"137","author":"Kim","year":"2015","journal-title":"Int J Cancer"},{"key":"10.1038\/modpathol.2015.144_bib54","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/s00281-007-0082-3","article-title":"Regulatory molecules involved in inflammasome formation with special reference to a key mediator protein, ASC","volume":"29","author":"Taniguchi","year":"2007","journal-title":"Semin Immunopathol"},{"key":"10.1038\/modpathol.2015.144_bib55","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1002\/ijc.23143","article-title":"Methylated TMS1 and DAPK genes predict prognosis and response to chemotherapy in gastric cancer","volume":"122","author":"Kato","year":"2008","journal-title":"Int J Cancer"},{"key":"10.1038\/modpathol.2015.144_bib56","doi-asserted-by":"crossref","first-page":"1822","DOI":"10.1111\/j.1349-7006.2010.01610.x","article-title":"Activation of ASC induces apoptosis or necrosis, depending on the cell type, and causes tumor eradication","volume":"101","author":"Motani","year":"2010","journal-title":"Cancer Sci"}],"container-title":["Modern Pathology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/modpathol2015144.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/modpathol2015144","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0893395222013096?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0893395222013096?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/modpathol2015144.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T00:47:30Z","timestamp":1760230050000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0893395222013096"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,2]]},"references-count":56,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,2]]}},"alternative-id":["S0893395222013096"],"URL":"https:\/\/doi.org\/10.1038\/modpathol.2015.144","relation":{},"ISSN":["0893-3952"],"issn-type":[{"value":"0893-3952","type":"print"}],"subject":[],"published":{"date-parts":[[2016,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"High-definition CpG methylation of novel genes in gastric carcinogenesis identified by next-generation sequencing","name":"articletitle","label":"Article Title"},{"value":"Modern Pathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1038\/modpathol.2015.144","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 United States & Canadian Academy of Pathology.","name":"copyright","label":"Copyright"}]}}