{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:07:14Z","timestamp":1791173234685,"version":"4.1.0"},"reference-count":44,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,10,7]],"date-time":"2016-10-07T00:00:00Z","timestamp":1475798400000},"content-version":"vor","delay-in-days":1041,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Therapy"],"published-print":{"date-parts":[[2013,12]]},"DOI":"10.1038\/mt.2013.184","type":"journal-article","created":{"date-parts":[[2013,8,13]],"date-time":"2013-08-13T11:15:22Z","timestamp":1376392522000},"page":"2205-2216","source":"Crossref","is-referenced-by-count":59,"title":["Oversized AAV Transductifon Is Mediated via a DNA-PKcs-independent, Rad51C-dependent Repair Pathway"],"prefix":"10.1016","volume":"21","author":[{"given":"Matthew L","family":"Hirsch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengwen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabella","family":"Bellon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoying","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sai","family":"Chavala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Pryadkina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Richard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard Jude","family":"Samulski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1038\/mt.2013.184_bib1","doi-asserted-by":"crossref","first-page":"2077","DOI":"10.1073\/pnas.79.6.2077","article-title":"Cloning of adeno-associated virus into pBR322: rescue of intact virus from the recombinant plasmid in human cells","volume":"79","author":"Samulski","year":"1982","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/mt.2013.184_bib2","doi-asserted-by":"crossref","first-page":"319","DOI":"10.2174\/156652310793180706","article-title":"AAV\u2019s anatomy: roadmap for optimizing vectors for translational success","volume":"10","author":"Mitchell","year":"2010","journal-title":"Curr Gene Ther"},{"key":"10.1038\/mt.2013.184_bib3","doi-asserted-by":"crossref","first-page":"1438","DOI":"10.1128\/JVI.72.2.1438-1445.1998","article-title":"Membrane-associated heparan sulfate proteoglycan is a receptor for adeno-associated virus type 2 virions","volume":"72","author":"Summerford","year":"1998","journal-title":"J Virol"},{"key":"10.1038\/mt.2013.184_bib4","doi-asserted-by":"crossref","first-page":"10371","DOI":"10.1128\/JVI.73.12.10371-10376.1999","article-title":"Dynamin is required for recombinant adeno-associated virus type 2 infection","volume":"73","author":"Duan","year":"1999","journal-title":"J Virol"},{"key":"10.1038\/mt.2013.184_bib5","doi-asserted-by":"crossref","first-page":"10346","DOI":"10.1128\/JVI.00841-06","article-title":"Host cell DNA repair pathways in adeno-associated viral genome processing","volume":"80","author":"Choi","year":"2006","journal-title":"J Virol"},{"key":"10.1038\/mt.2013.184_bib6","doi-asserted-by":"crossref","first-page":"12279","DOI":"10.1128\/JVI.75.24.12279-12287.2001","article-title":"Involvement of cellular double-stranded DNA break binding proteins in processing of the recombinant adeno-associated virus genome","volume":"75","author":"Zentilin","year":"2001","journal-title":"J Virol"},{"key":"10.1038\/mt.2013.184_bib7","doi-asserted-by":"crossref","first-page":"4751","DOI":"10.1128\/JVI.77.8.4751-4759.2003","article-title":"Consequences of DNA-dependent protein kinase catalytic subunit deficiency on recombinant adeno-associated virus genome circularization and heterodimerization in muscle tissue","volume":"77","author":"Duan","year":"2003","journal-title":"J Virol"},{"key":"10.1038\/mt.2013.184_bib8","doi-asserted-by":"crossref","first-page":"8673","DOI":"10.1128\/JVI.00641-10","article-title":"Differential effects of DNA double-strand break repair pathways on single-strand and self-complementary adeno-associated virus vector genomes","volume":"84","author":"Cataldi","year":"2010","journal-title":"J Virol"},{"key":"10.1038\/mt.2013.184_bib9","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.1038\/mt.2012.144","article-title":"Terminal differentiation of cardiac and skeletal myocytes induces permissivity to AAV transduction by relieving inhibition imposed by DNA damage response proteins","volume":"20","author":"Lovric","year":"2012","journal-title":"Mol Ther"},{"key":"10.1038\/mt.2013.184_bib10","doi-asserted-by":"crossref","first-page":"e7705","DOI":"10.1371\/journal.pone.0007705","article-title":"AAV recombineering with single strand oligonucleotides","volume":"4","author":"Hirsch","year":"2009","journal-title":"PLoS ONE"},{"key":"10.1038\/mt.2013.184_bib11","doi-asserted-by":"crossref","first-page":"11304","DOI":"10.1128\/JVI.01225-07","article-title":"The role of DNA-PKcs and artemis in opening viral DNA hairpin termini in various tissues in mice","volume":"81","author":"Inagaki","year":"2007","journal-title":"J Virol"},{"key":"10.1038\/mt.2013.184_bib12","doi-asserted-by":"crossref","first-page":"e27520","DOI":"10.1371\/journal.pone.0027520","article-title":"Viral single-strand DNA induces p53-dependent apoptosis in human embryonic stem cells","volume":"6","author":"Hirsch","year":"2011","journal-title":"PLoS ONE"},{"key":"10.1038\/mt.2013.184_bib13","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1038\/35091082","article-title":"Virus-mediated killing of cells that lack p53 activity","volume":"412","author":"Raj","year":"2001","journal-title":"Nature"},{"key":"10.1038\/mt.2013.184_bib14","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1006\/mthe.2001.0456","article-title":"Expanding AAV packaging capacity with trans-splicing or overlapping vectors: a quantitative comparison","volume":"4","author":"Duan","year":"2001","journal-title":"Mol Ther"},{"key":"10.1038\/mt.2013.184_bib15","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1038\/nbt0702-697","article-title":"Efficient mouse airway transduction following recombination between AAV vectors carrying parts of a larger gene","volume":"20","author":"Halbert","year":"2002","journal-title":"Nat Biotechnol"},{"key":"10.1038\/mt.2013.184_bib16","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1089\/hum.2010.122","article-title":"Efficient transgene reconstitution with hybrid dual AAV vectors carrying the minimized bridging sequences","volume":"22","author":"Ghosh","year":"2011","journal-title":"Hum Gene Ther"},{"key":"10.1038\/mt.2013.184_bib17","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.1172\/JCI34316","article-title":"Serotype-dependent packaging of large genes in adeno-associated viral vectors results in effective gene delivery in mice","volume":"118","author":"Allocca","year":"2008","journal-title":"J Clin Invest"},{"key":"10.1038\/mt.2013.184_bib18","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1038\/mt.2009.255","article-title":"Effect of genome size on AAV vector packaging","volume":"18","author":"Wu","year":"2010","journal-title":"Mol Ther"},{"key":"10.1038\/mt.2013.184_bib19","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1038\/mt.2009.258","article-title":"Characterization of genome integrity for oversized recombinant AAV vector","volume":"18","author":"Dong","year":"2010","journal-title":"Mol Ther"},{"key":"10.1038\/mt.2013.184_bib20","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1038\/mt.2009.256","article-title":"Evidence for the failure of adeno-associated virus serotype 5 to package a viral genome > or = 8.2\u2009kb","volume":"18","author":"Lai","year":"2010","journal-title":"Mol Ther"},{"key":"10.1038\/mt.2013.184_bib21","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1089\/hum.2011.160","article-title":"Native molecular state of adeno-associated viral vectors revealed by single-molecule sequencing","volume":"23","author":"Kapranov","year":"2012","journal-title":"Hum Gene Ther"},{"key":"10.1038\/mt.2013.184_bib22","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1038\/mt.2009.280","article-title":"Little vector, big gene transduction: fragmented genome reassembly of adeno-associated virus","volume":"18","author":"Hirsch","year":"2010","journal-title":"Mol Ther"},{"key":"10.1038\/mt.2013.184_bib23","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.1038\/mt.2010.170","article-title":"Proteasome inhibitors enhance gene delivery by AAV virus vectors expressing large genomes in hemophilia mouse and dog models: a strategy for broad clinical application","volume":"18","author":"Monahan","year":"2010","journal-title":"Mol Ther"},{"key":"10.1038\/mt.2013.184_bib24","doi-asserted-by":"crossref","first-page":"e39233","DOI":"10.1371\/journal.pone.0039233","article-title":"Homologous recombination mediates functional recovery of dysferlin deficiency following AAV5 gene transfer","volume":"7","author":"Grose","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1038\/mt.2013.184_bib25","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1038\/nprot.2006.207","article-title":"Production and characterization of adeno-associated viral vectors","volume":"1","author":"Grieger","year":"2006","journal-title":"Nat Protoc"},{"key":"10.1038\/mt.2013.184_bib26","doi-asserted-by":"crossref","first-page":"3241","DOI":"10.1128\/JVI.72.4.3241-3247.1998","article-title":"In vitro packaging of adeno-associated virus DNA","volume":"72","author":"Zhou","year":"1998","journal-title":"J Virol"},{"key":"10.1038\/mt.2013.184_bib27","doi-asserted-by":"crossref","first-page":"10770","DOI":"10.1073\/pnas.0902269106","article-title":"Cellular immune response to cryptic epitopes during therapeutic gene transfer","volume":"106","author":"Li","year":"2009","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/mt.2013.184_bib28","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1093\/hmg\/ddq065","article-title":"Efficient recovery of dysferlin deficiency by dual adeno-associated vector-mediated gene transfer","volume":"19","author":"Lostal","year":"2010","journal-title":"Hum Mol Genet"},{"key":"10.1038\/mt.2013.184_bib29","doi-asserted-by":"crossref","first-page":"3171","DOI":"10.1073\/pnas.92.8.3171","article-title":"Loss of the catalytic subunit of the DNA-dependent protein kinase in DNA double-strand-break-repair mutant mammalian cells","volume":"92","author":"Peterson","year":"1995","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/mt.2013.184_bib30","doi-asserted-by":"crossref","first-page":"5836","DOI":"10.1128\/MCB.23.16.5836-5848.2003","article-title":"Autophosphorylation of the catalytic subunit of the DNA-dependent protein kinase is required for efficient end processing during DNA double-strand break repair","volume":"23","author":"Ding","year":"2003","journal-title":"Mol Cell Biol"},{"key":"10.1038\/mt.2013.184_bib31","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1126\/science.1093037","article-title":"RAD51C is required for Holliday junction processing in mammalian cells","volume":"303","author":"Liu","year":"2004","journal-title":"Science"},{"key":"10.1038\/mt.2013.184_bib32","doi-asserted-by":"crossref","first-page":"3296","DOI":"10.1101\/gad.947001","article-title":"Identification and purification of two distinct complexes containing the five RAD51 paralogs","volume":"15","author":"Masson","year":"2001","journal-title":"Genes Dev"},{"key":"10.1038\/mt.2013.184_bib33","doi-asserted-by":"crossref","first-page":"9933","DOI":"10.1128\/JVI.79.15.9933-9944.2005","article-title":"Packaging capacity of adeno-associated virus serotypes: impact of larger genomes on infectivity and postentry steps","volume":"79","author":"Grieger","year":"2005","journal-title":"J Virol"},{"key":"10.1038\/mt.2013.184_bib34","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1089\/hum.2004.15.896","article-title":"Trans-splicing adeno-associated viral vector-mediated gene therapy is limited by the accumulation of spliced mRNA but not by dual vector coinfection efficiency","volume":"15","author":"Xu","year":"2004","journal-title":"Hum Gene Ther"},{"key":"10.1038\/mt.2013.184_bib35","doi-asserted-by":"crossref","first-page":"31945","DOI":"10.1074\/jbc.M109.024646","article-title":"Cellular redistribution of Rad51 in response to DNA damage: novel role for Rad51C","volume":"284","author":"Gildemeister","year":"2009","journal-title":"J Biol Chem"},{"key":"10.1038\/mt.2013.184_bib36","doi-asserted-by":"crossref","first-page":"8406","DOI":"10.1074\/jbc.M108306200","article-title":"RAD51C interacts with RAD51B and is central to a larger protein complex in vivo exclusive of RAD51","volume":"277","author":"Miller","year":"2002","journal-title":"J Biol Chem"},{"key":"10.1038\/mt.2013.184_bib37","doi-asserted-by":"crossref","first-page":"8440","DOI":"10.1073\/pnas.111005698","article-title":"Complex formation by the human RAD51C and XRCC3 recombination repair proteins","volume":"98","author":"Masson","year":"2001","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/mt.2013.184_bib38","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1093\/nar\/30.4.1001","article-title":"Interactions involving the Rad51 paralogs Rad51C and XRCC3 in human cells","volume":"30","author":"Wiese","year":"2002","journal-title":"Nucleic Acids Res"},{"key":"10.1038\/mt.2013.184_bib39","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1038\/sj.mt.6300355","article-title":"Optimization of self-complementary AAV vectors for liver-directed expression results in sustained correction of hemophilia B at low vector dose","volume":"16","author":"Wu","year":"2008","journal-title":"Mol Ther"},{"key":"10.1038\/mt.2013.184_bib40","doi-asserted-by":"crossref","first-page":"1825","DOI":"10.1038\/mt.2008.186","article-title":"Codon and mRNA sequence optimization of microdystrophin transgenes improves expression and physiological outcome in dystrophic mdx mice following AAV2\/8 gene transfer","volume":"16","author":"Foster","year":"2008","journal-title":"Mol Ther"},{"key":"10.1038\/mt.2013.184_bib41","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.2353\/ajpath.2009.090107","article-title":"Genetic manipulation of dysferlin expression in skeletal muscle: novel insights into muscular dystrophy","volume":"175","author":"Millay","year":"2009","journal-title":"Am J Pathol"},{"key":"10.1038\/mt.2013.184_bib42","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1002\/ana.21926","article-title":"Dysferlin overexpression in skeletal muscle produces a progressive myopathy","volume":"67","author":"Glover","year":"2010","journal-title":"Ann Neurol"},{"key":"10.1038\/mt.2013.184_bib43","first-page":"279","article-title":"Isolation and cross-sensitivity of X-ray-sensitive mutants of V79-4 hamster cells","volume":"183","author":"Jones","year":"1987","journal-title":"Mutat Res"},{"key":"10.1038\/mt.2013.184_bib44","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/0921-8777(91)90046-R","article-title":"Cross-sensitivity of gamma-ray-sensitive hamster mutants to cross-linking agents","volume":"255","author":"Caldecott","year":"1991","journal-title":"Mutat Res"}],"container-title":["Molecular Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525001616309467?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525001616309467?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T12:51:06Z","timestamp":1715863866000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1525001616309467"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12]]},"references-count":44,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["S1525001616309467"],"URL":"https:\/\/doi.org\/10.1038\/mt.2013.184","relation":{},"ISSN":["1525-0016"],"issn-type":[{"value":"1525-0016","type":"print"}],"subject":[],"published":{"date-parts":[[2013,12]]}}}