{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T21:58:41Z","timestamp":1785880721484,"version":"3.56.0"},"reference-count":42,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,10,7]],"date-time":"2016-10-07T00:00:00Z","timestamp":1475798400000},"content-version":"vor","delay-in-days":614,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Therapy"],"published-print":{"date-parts":[[2015,2]]},"DOI":"10.1038\/mt.2014.222","type":"journal-article","created":{"date-parts":[[2014,11,13]],"date-time":"2014-11-13T17:33:21Z","timestamp":1415900001000},"page":"278-289","source":"Crossref","is-referenced-by-count":34,"title":["Cyclooxygenase-2 Silencing for the Treatment of Colitis: A Combined In Vivo Strategy Based on RNA Interference and Engineered Escherichia Coli"],"prefix":"10.1016","volume":"23","author":[{"given":"Enzo","family":"Spisni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria C","family":"Valerii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigia","family":"De Fazio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Cavazza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Borsetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annamaria","family":"Sgromo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Candela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Centanni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Rizello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Strillacci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1038\/mt.2014.222_bib1","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1038\/nbt0998-862","article-title":"Functional gene transfer from intracellular bacteria to mammalian cells","volume":"16","author":"Grillot-Courvalin","year":"1998","journal-title":"Nat Biotechnol"},{"key":"10.1038\/mt.2014.222_bib2","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.1038\/sj.gt.3302493","article-title":"Engineered E. coli delivers therapeutic genes to the colonic mucosa","volume":"12","author":"Castagliuolo","year":"2005","journal-title":"Gene Ther"},{"key":"10.1038\/mt.2014.222_bib3","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.ymthe.2006.01.011","article-title":"Recombinant Escherichia coli expressing invasin targets the Peyer's patches: the basis for a bacterial formulation for oral vaccination","volume":"14","author":"Critchley-Thorne","year":"2006","journal-title":"Mol Ther"},{"key":"10.1038\/mt.2014.222_bib4","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1038\/nbt1211","article-title":"Short hairpin RNA-expressing bacteria elicit RNA interference in mammals","volume":"24","author":"Xiang","year":"2006","journal-title":"Nat Biotechnol"},{"key":"10.1038\/mt.2014.222_bib5","series-title":"Tumour Angiogenesis","first-page":"291","article-title":"Involvement of prostanoid in angiogenesis","author":"Spisni","year":"1997"},{"key":"10.1038\/mt.2014.222_bib6","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1038\/sj.bjc.6603094","article-title":"RNA interference as a key to knockdown overexpressed cyclooxygenase-2 gene in tumour cells","volume":"94","author":"Strillacci","year":"2006","journal-title":"Br J Cancer"},{"issue":"9B","key":"10.1038\/mt.2014.222_bib7","doi-asserted-by":"crossref","first-page":"3876","DOI":"10.1111\/j.1582-4934.2008.00580.x","article-title":"Cyclooxygenase-2\/carbonic anhydrase-IX up-regulation promotes invasive potential and hypoxia survival in colorectal cancer cells","volume":"13","author":"Sansone","year":"2009","journal-title":"J Cell Mol Med"},{"key":"10.1038\/mt.2014.222_bib8","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1056\/NEJMoa050405","article-title":"Cardiovascular risk associated with celecoxib in a clinical trial for colorectal adenoma prevention","volume":"352","author":"Solomon","year":"2005","journal-title":"N Engl J Med"},{"key":"10.1038\/mt.2014.222_bib9","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1056\/NEJMoa050493","article-title":"Cardiovascular events associated with rofecoxib in a colorectal adenoma chemoprevention trial","volume":"352","author":"Bresalier","year":"2005","journal-title":"N Engl J Med"},{"key":"10.1038\/mt.2014.222_bib10","doi-asserted-by":"crossref","first-page":"828045","DOI":"10.1155\/2010\/828045","article-title":"RNAi-based strategies for cyclooxygenase-2 inhibition in cancer","volume":"2010","author":"Strillacci","year":"2010","journal-title":"J Biomed Biotechnol"},{"key":"10.1038\/mt.2014.222_bib11","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1038\/sj.bjc.6605859","article-title":"Selective cyclooxygenase-2 silencing mediated by engineered E. coli and RNA interference induces anti-tumour effects in human colon cancer cells","volume":"103","author":"Strillacci","year":"2010","journal-title":"Br J Cancer"},{"key":"10.1038\/mt.2014.222_bib12","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/S0016-5085(98)70196-9","article-title":"Cyclooxygenase 2 is induced in colonic epithelial cells in inflammatory bowel disease","volume":"115","author":"Singer","year":"1998","journal-title":"Gastroenterology"},{"key":"10.1038\/mt.2014.222_bib13","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/S0016-5085(98)70381-6","article-title":"Inflammatory bowel disease: etiology and pathogenesis","volume":"115","author":"Fiocchi","year":"1998","journal-title":"Gastroenterology"},{"key":"10.1038\/mt.2014.222_bib14","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1002\/1097-0142(20010215)91:4<854::AID-CNCR1073>3.0.CO;2-Z","article-title":"Cancer risk in patients with inflammatory bowel disease: a population-based study","volume":"91","author":"Bernstein","year":"2001","journal-title":"Cancer"},{"key":"10.1038\/mt.2014.222_bib15","doi-asserted-by":"crossref","first-page":"10766","DOI":"10.1158\/0008-5472.CAN-07-1418","article-title":"Prolonged chronic inflammation progresses to dysplasia in a novel rat model of colitis-associated colon cancer","volume":"67","author":"Santiago","year":"2007","journal-title":"Cancer Res"},{"key":"10.1038\/mt.2014.222_bib16","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.pharmthera.2012.10.008","article-title":"Immune aspects of the pathogenesis of inflammatory bowel disease","volume":"137","author":"Hisamatsu","year":"2013","journal-title":"Pharmacol Ther"},{"key":"10.1038\/mt.2014.222_bib17","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.autrev.2013.06.004","article-title":"Innate and adaptive immunity in inflammatory bowel disease","volume":"13","author":"Geremia","year":"2014","journal-title":"Autoimmun Rev"},{"key":"10.1038\/mt.2014.222_bib18","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1038\/nri3661","article-title":"Cytokines in inflammatory bowel disease","volume":"14","author":"Neurath","year":"2014","journal-title":"Nat Rev Immunol"},{"key":"10.1038\/mt.2014.222_bib19","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1038\/nrmicro2403","article-title":"The impact of the microbiota on the pathogenesis of IBD: lessons from mouse infection models","volume":"8","author":"Nell","year":"2010","journal-title":"Nat Rev Microbiol"},{"key":"10.1038\/mt.2014.222_bib20","doi-asserted-by":"crossref","first-page":"170","DOI":"10.5009\/gnl.2014.8.2.170","article-title":"Relationship between the severity of diversion colitis and the composition of colonic bacteria: a prospective study","volume":"8","author":"Baek","year":"2014","journal-title":"Gut Liver"},{"key":"10.1038\/mt.2014.222_bib21","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1038\/nri3430","article-title":"Role of the gut microbiota in immunity and inflammatory disease","volume":"13","author":"Kamada","year":"2013","journal-title":"Nat Rev Immunol"},{"key":"10.1038\/mt.2014.222_bib22","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S1734-1140(11)70575-8","article-title":"Treatment of inflammatory bowel disease (IBD)","volume":"63","author":"Pithadia","year":"2011","journal-title":"Pharmacol Rep"},{"key":"10.1038\/mt.2014.222_bib23","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1038\/mt.2011.236","article-title":"Functionally enhanced siRNA targeting TNFa attenuates DSS-induced colitis and TLR-mediated immunostimulation in mice","volume":"20","author":"Ocampo","year":"2012","journal-title":"Mol Ther"},{"key":"10.1038\/mt.2014.222_bib24","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1038\/mt.2012.24","article-title":"A new therapeutic approach using a schizophyllan-based drug delivery system for inflammatory bowel disease","volume":"20","author":"Takedatsu","year":"2012","journal-title":"Mol Ther"},{"key":"10.1038\/mt.2014.222_bib25","doi-asserted-by":"crossref","first-page":"1352","DOI":"10.1126\/science.289.5483.1352","article-title":"Treatment of murine colitis by Lactococcus lactis secreting interleukin-10","volume":"289","author":"Steidler","year":"2000","journal-title":"Science"},{"key":"10.1038\/mt.2014.222_bib26","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/nbt840","article-title":"Biological containment of genetically modified Lactococcus lactis for intestinal delivery of human interleukin 10","volume":"21","author":"Steidler","year":"2003","journal-title":"Nat Biotechnol"},{"key":"10.1038\/mt.2014.222_bib27","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1038\/nprot.2007.41","article-title":"Chemically induced mouse models of intestinal inflammation","volume":"2","author":"Wirtz","year":"2007","journal-title":"Nat Protoc"},{"key":"10.1038\/mt.2014.222_bib28","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.3892\/or.2012.1969","article-title":"Dextran sulfate sodium administered orally is depolymerized in the stomach and induces cell cycle arrest plus apoptosis in the colon in early mouse colitis","volume":"28","author":"Araki","year":"2012","journal-title":"Oncol Rep"},{"key":"10.1038\/mt.2014.222_bib29","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1016\/j.bcp.2012.09.007","article-title":"Evaluation of chemical mediators and cellular response during acute and chronic gut inflammatory response induced by dextran sodium sulfate in mice","volume":"84","author":"Bento","year":"2012","journal-title":"Biochem Pharmacol"},{"key":"10.1038\/mt.2014.222_bib30","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1007\/s12275-012-1362-8","article-title":"Microbial fingerprinting detects unique bacterial communities in the faecal microbiota of rats with experimentally-induced colitis","volume":"50","author":"Samanta","year":"2012","journal-title":"J Microbiol"},{"key":"10.1038\/mt.2014.222_bib31","doi-asserted-by":"crossref","first-page":"2091","DOI":"10.1038\/ismej.2012.39","article-title":"Phylotype-level 16S rRNA analysis reveals new bacterial indicators of health state in acute murine colitis","volume":"6","author":"Berry","year":"2012","journal-title":"ISME J"},{"key":"10.1038\/mt.2014.222_bib32","doi-asserted-by":"crossref","first-page":"2051","DOI":"10.3748\/wjg.v20.i8.2051","article-title":"Longitudinal analysis of inflammation and microbiota dynamics in a model of mild chronic dextran sulfate sodium-induced colitis in mice","volume":"20","author":"De Fazio","year":"2014","journal-title":"World J Gastroenterol"},{"key":"10.1038\/mt.2014.222_bib33","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1172\/JCI117060","article-title":"Cyclooxygenase-1 and -2 expression in rheumatoid synovial tissues. Effects of interleukin-1 beta, phorbol ester, and corticosteroids","volume":"93","author":"Crofford","year":"1994","journal-title":"J Clin Invest"},{"key":"10.1038\/mt.2014.222_bib34","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/1471-2172-13-41","article-title":"Multifunctional role of dextran sulfate sodium for in vivo modeling of intestinal diseases","volume":"13","author":"Rose","year":"2012","journal-title":"BMC Immunol"},{"key":"10.1038\/mt.2014.222_bib35","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1002\/ibd.20753","article-title":"Distinct cytokine patterns identified from multiplex profiles of murine DSS and TNBS-induced colitis","volume":"15","author":"Alex","year":"2009","journal-title":"Inflamm Bowel Dis"},{"key":"10.1038\/mt.2014.222_bib36","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.cellimm.2004.04.003","article-title":"Inhibition of IL-6, TNF-a and cyclooxygenase-2 protein expression by prostglandine E2-induced IL-10 in bone marrow-derivade dendritic cells","volume":"228","author":"Hedi","year":"2004","journal-title":"Cellular Immun"},{"key":"10.1038\/mt.2014.222_bib37","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/S0140-6736(98)06343-0","article-title":"Non-pathogenic Escherichia coli versus mesalazine for the treatment of ulcerative colitis: a randomised trial","volume":"354","author":"Rembacken","year":"1999","journal-title":"Lancet"},{"key":"10.1038\/mt.2014.222_bib38","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1136\/gut.2003.037747","article-title":"Maintaining remission of ulcerative colitis with the probiotic Escherichia coli Nissle 1917 is as effective as with standard mesalazine","volume":"53","author":"Kruis","year":"2004","journal-title":"Gut"},{"key":"10.1038\/mt.2014.222_bib39","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/S1567-5769(02)00299-0","article-title":"Possible role of mucosal mast cells in the recovery process of colitis induced by dextran sulfate sodium in rats","volume":"3","author":"Iba","year":"2003","journal-title":"Int Immunopharmacol"},{"key":"10.1038\/mt.2014.222_bib40","doi-asserted-by":"crossref","first-page":"7161","DOI":"10.1128\/AEM.70.12.7161-7172.2004","article-title":"Development of a universal microarray based on the ligation detection reaction and 16S rrna gene polymorphism to target diversity of cyanobacteria","volume":"70","author":"Castiglioni","year":"2004","journal-title":"Appl Environ Microbiol"},{"key":"10.1038\/mt.2014.222_bib41","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1186\/1471-2180-12-95","article-title":"Unbalance of intestinal microbiota in atopic children","volume":"12","author":"Candela","year":"2012","journal-title":"BMC Microbiol"},{"key":"10.1038\/mt.2014.222_bib42","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1021\/bc050285a","article-title":"A structured chitosan-based platform for biomolecule attachment to solid surfaces: application to DNA microarray preparation","volume":"17","author":"Consolandi","year":"2006","journal-title":"Bioconjug Chem"}],"container-title":["Molecular Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525001616300387?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525001616300387?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,8,17]],"date-time":"2019-08-17T09:15:49Z","timestamp":1566033349000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1525001616300387"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2]]},"references-count":42,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["S1525001616300387"],"URL":"https:\/\/doi.org\/10.1038\/mt.2014.222","relation":{},"ISSN":["1525-0016"],"issn-type":[{"value":"1525-0016","type":"print"}],"subject":[],"published":{"date-parts":[[2015,2]]}}}