{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:00:23Z","timestamp":1792713623765,"version":"4.5.0"},"reference-count":18,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,2,18]],"date-time":"2017-02-18T00:00:00Z","timestamp":1487376000000},"content-version":"vor","delay-in-days":414,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Therapy - Methods & Clinical Development"],"published-print":{"date-parts":[[2016]]},"DOI":"10.1038\/mtm.2016.46","type":"journal-article","created":{"date-parts":[[2016,7,13]],"date-time":"2016-07-13T12:16:29Z","timestamp":1468412189000},"page":"16046","source":"Crossref","is-referenced-by-count":49,"special_numbering":"C","title":["Potent spinal parenchymal AAV9-mediated gene delivery by subpial injection in adult rats and pigs"],"prefix":"10.1016","volume":"3","author":[{"given":"Atsushi","family":"Miyanohara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kota","family":"Kamizato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Juhas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Juhasova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Navarro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Marsala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nada","family":"Lukacova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marian","family":"Hruska-Plochan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Curtis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandon","family":"Gabel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Ciacci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric T","family":"Ahrens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian K","family":"Kaspar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Don","family":"Cleveland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Marsala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1038\/mtm.2016.46_bib1","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nbt.1515","article-title":"Intravascular AAV9 preferentially targets neonatal neurons and adult astrocytes","volume":"27","author":"Foust","year":"2009","journal-title":"Nat Biotechnol"},{"key":"10.1038\/mtm.2016.46_bib2","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1038\/mt.2009.71","article-title":"Intravenous administration of self-complementary AAV9 enables transgene delivery to adult motor neurons","volume":"17","author":"Duque","year":"2009","journal-title":"Mol Ther"},{"key":"10.1038\/mtm.2016.46_bib3","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1038\/mt.2011.72","article-title":"Preclinical differences of intravascular AAV9 delivery to neurons and glia: a comparative study of adult mice and nonhuman primates","volume":"19","author":"Gray","year":"2011","journal-title":"Mol Ther"},{"key":"10.1038\/mtm.2016.46_bib4","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1038\/mt.2014.210","article-title":"Improving single injection CSF delivery of AAV9-mediated gene therapy for SMA: a dose-response study in mice and nonhuman primates","volume":"23","author":"Meyer","year":"2015","journal-title":"Mol Ther"},{"key":"10.1038\/mtm.2016.46_bib5","doi-asserted-by":"crossref","first-page":"2148","DOI":"10.1038\/mt.2013.211","article-title":"Therapeutic AAV9-mediated suppression of mutant SOD1 slows disease progression and extends survival in models of inherited ALS","volume":"21","author":"Foust","year":"2013","journal-title":"Mol Ther"},{"key":"10.1038\/mtm.2016.46_bib6","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1089\/hum.2014.011","article-title":"Translational fidelity of intrathecal delivery of self-complementary AAV9-survival motor neuron 1 for spinal muscular atrophy","volume":"25","author":"Passini","year":"2014","journal-title":"Hum Gene Ther"},{"key":"10.1038\/mtm.2016.46_bib7","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1089\/hgtb.2015.041","article-title":"Motor neuron transduction after intracisternal delivery of AAV9 in a cynomolgus macaque","volume":"26","author":"Bell","year":"2015","journal-title":"Hum Gene Ther Methods"},{"key":"10.1038\/mtm.2016.46_bib8","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/BF00454899","article-title":"Patterns of neuronal degeneration in the motor cortex of amyotrophic lateral sclerosis patients","volume":"86","author":"Nihei","year":"1993","journal-title":"Acta Neuropathol"},{"key":"10.1038\/mtm.2016.46_bib9","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/0361-9230(93)90267-F","article-title":"Selective vulnerability of alpha motor neurons in ALS: relation to autoantibodies toward acetylcholinesterase (AChE) in ALS patients","volume":"30","author":"Conradi","year":"1993","journal-title":"Brain Res Bull"},{"key":"10.1038\/mtm.2016.46_bib10","doi-asserted-by":"crossref","first-page":"e42614","DOI":"10.1371\/journal.pone.0042614","article-title":"Human neural stem cell replacement therapy for amyotrophic lateral sclerosis by spinal transplantation","volume":"7","author":"Hefferan","year":"2012","journal-title":"PLoS One"},{"key":"10.1038\/mtm.2016.46_bib11","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1038\/mt.2013.266","article-title":"AAV9-mediated expression of a non-self protein in nonhuman primate central nervous system triggers widespread neuroinflammation driven by antigen-presenting cell transduction","volume":"22","author":"Samaranch","year":"2014","journal-title":"Mol Ther"},{"key":"10.1038\/mtm.2016.46_bib12","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.expneurol.2004.07.014","article-title":"Region-specific cell grafting into cervical and lumbar spinal cord in rat: a qualitative and quantitative stereological study","volume":"190","author":"Kakinohana","year":"2004","journal-title":"Exp Neurol"},{"key":"10.1038\/mtm.2016.46_bib13","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.3727\/096368910X503406","article-title":"Analysis of dosing regimen and reproducibility of intraspinal grafting of human spinal stem cells in immunosuppressed minipigs","volume":"19","author":"Usvald","year":"2010","journal-title":"Cell Transplant"},{"key":"10.1038\/mtm.2016.46_bib14","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1126\/science.1273597","article-title":"Analgesia mediated by a direct spinal action of narcotics","volume":"192","author":"Yaksh","year":"1976","journal-title":"Science"},{"key":"10.1038\/mtm.2016.46_bib15","doi-asserted-by":"crossref","first-page":"e32581","DOI":"10.1371\/journal.pone.0032581","article-title":"In vivo gene knockdown in rat dorsal root ganglia mediated by self-complementary adeno-associated virus serotype 5 following intrathecal delivery","volume":"7","author":"Xu","year":"2012","journal-title":"PLoS One"},{"key":"10.1038\/mtm.2016.46_bib16","doi-asserted-by":"crossref","first-page":"2224","DOI":"10.1128\/JVI.72.3.2224-2232.1998","article-title":"Production of high-titer recombinant adeno-associated virus vectors in the absence of helper adenovirus","volume":"72","author":"Xiao","year":"1998","journal-title":"J Virol"},{"key":"10.1038\/mtm.2016.46_bib17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1089\/neu.1995.12.1","article-title":"A sensitive and reliable locomotor rating scale for open field testing in rats","volume":"12","author":"Basso","year":"1995","journal-title":"J Neurotrauma"},{"key":"10.1038\/mtm.2016.46_bib18","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1089\/neu.2011.2076","article-title":"Chronic spinal compression model in minipigs: a systematic behavioral, qualitative, and quantitative neuropathological study","volume":"29","author":"Navarro","year":"2012","journal-title":"J Neurotrauma"}],"container-title":["Molecular Therapy - Methods & Clinical Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2329050117300189?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2329050117300189?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T18:33:02Z","timestamp":1600885982000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2329050117300189"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":18,"alternative-id":["S2329050117300189"],"URL":"https:\/\/doi.org\/10.1038\/mtm.2016.46","relation":{},"ISSN":["2329-0501"],"issn-type":[{"value":"2329-0501","type":"print"}],"subject":[],"published":{"date-parts":[[2016]]},"article-number":"16046"}}