{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T07:30:45Z","timestamp":1773559845014,"version":"3.50.1"},"reference-count":54,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2017,1,25]],"date-time":"2017-01-25T00:00:00Z","timestamp":1485302400000},"content-version":"vor","delay-in-days":390,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Therapy - Nucleic Acids"],"published-print":{"date-parts":[[2016]]},"DOI":"10.1038\/mtna.2016.20","type":"journal-article","created":{"date-parts":[[2016,4,12]],"date-time":"2016-04-12T11:25:58Z","timestamp":1460460358000},"page":"e308","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Inhibition of Influenza Virus Replication by DNA Aptamers Targeting a Cellular Component of Translation Initiation"],"prefix":"10.1016","volume":"5","author":[{"given":"Paloma","family":"Rodriguez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M Isabel","family":"P\u00e9rez-Morgado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00edctor M","family":"Gonzalez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M Elena","family":"Mart\u00edn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amelia","family":"Nieto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1038\/mtna.2016.20_bib1","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1128\/jvi.59.2.377-383.1986","article-title":"Measurement of the mutation rates of animal viruses: influenza A virus and poliovirus type 1","volume":"59","author":"Parvin","year":"1986","journal-title":"J Virol"},{"key":"10.1038\/mtna.2016.20_bib2","doi-asserted-by":"crossref","first-page":"10417","DOI":"10.1016\/S0021-9258(18)67540-4","article-title":"Proofreading function associated with the RNA-dependent RNA polymerase from influenza virus","volume":"261","author":"Ishihama","year":"1986","journal-title":"J Biol Chem"},{"key":"10.1038\/mtna.2016.20_bib3","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1038\/nrmicro1208","article-title":"Influenza: lessons from past pandemics, warnings from current incidents","volume":"3","author":"Horimoto","year":"2005","journal-title":"Nat Rev Microbiol"},{"key":"10.1038\/mtna.2016.20_bib4","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1146\/annurev.med.51.1.407","article-title":"Global epidemiology of influenza: past and present","volume":"51","author":"Cox","year":"2000","journal-title":"Annu Rev Med"},{"key":"10.1038\/mtna.2016.20_bib5","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00201-12","article-title":"Reconstruction of the 1918 influenza virus: unexpected rewards from the past","volume":"3","author":"Taubenberger","year":"2012","journal-title":"MBio"},{"key":"10.1038\/mtna.2016.20_bib6","doi-asserted-by":"crossref","first-page":"1840","DOI":"10.1126\/science.1062882","article-title":"Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses","volume":"293","author":"Hatta","year":"2001","journal-title":"Science"},{"key":"10.1038\/mtna.2016.20_bib7","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1038\/nature08157","article-title":"Emergence and pandemic potential of swine-origin H1N1 influenza virus","volume":"459","author":"Neumann","year":"2009","journal-title":"Nature"},{"key":"10.1038\/mtna.2016.20_bib8","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.chom.2015.02.019","article-title":"Influenza surveillance: 2014-2015 H1N1 \u201cswine\u201d-derived influenza viruses from India","volume":"17","author":"Tharakaraman","year":"2015","journal-title":"Cell Host Microbe"},{"key":"10.1038\/mtna.2016.20_bib9","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/j.tim.2014.08.008","article-title":"Pandemic potential of avian influenza A (H7N9) viruses","volume":"22","author":"Watanabe","year":"2014","journal-title":"Trends Microbiol"},{"key":"10.1038\/mtna.2016.20_bib10","doi-asserted-by":"crossref","first-page":"8214","DOI":"10.1128\/JVI.72.10.8214-8219.1998","article-title":"The RNA polymerase of influenza virus, bound to the 5\u2019 end of virion RNA, acts in cis to polyadenylate mRNA","volume":"72","author":"Poon","year":"1998","journal-title":"J Virol"},{"key":"10.1038\/mtna.2016.20_bib11","doi-asserted-by":"crossref","first-page":"2109","DOI":"10.1128\/JVI.73.3.2109-2114.1999","article-title":"A hairpin loop at the 5\u2019 end of influenza A virus virion RNA is required for synthesis of poly(A)+ mRNA in vitro","volume":"73","author":"Pritlove","year":"1999","journal-title":"J Virol"},{"key":"10.1038\/mtna.2016.20_bib12","doi-asserted-by":"crossref","first-page":"3473","DOI":"10.1128\/JVI.73.4.3473-3476.1999","article-title":"Direct evidence that the poly(A) tail of influenza A virus mRNA is synthesized by reiterative copying of a U track in the virion RNA template","volume":"73","author":"Poon","year":"1999","journal-title":"J Virol"},{"key":"10.1038\/mtna.2016.20_bib13","doi-asserted-by":"crossref","first-page":"2861","DOI":"10.1128\/jvi.65.6.2861-2867.1991","article-title":"The polyadenylation signal of influenza virus RNA involves a stretch of uridines followed by the RNA duplex of the panhandle structure","volume":"65","author":"Luo","year":"1991","journal-title":"J Virol"},{"key":"10.1038\/mtna.2016.20_bib14","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1128\/jvi.60.3.1027-1039.1986","article-title":"Cellular mRNA translation is blocked at both initiation and elongation after infection by influenza virus or adenovirus","volume":"60","author":"Katze","year":"1986","journal-title":"J Virol"},{"key":"10.1038\/mtna.2016.20_bib15","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1146\/annurev.biochem.68.1.913","article-title":"eIF4 initiation factors: effectors of mRNA recruitment to ribosomes and regulators of translation","volume":"68","author":"Gingras","year":"1999","journal-title":"Annu Rev Biochem"},{"key":"10.1038\/mtna.2016.20_bib16","first-page":"3419","article-title":"The multiple RNA-binding domains of the mRNA poly(A)-binding protein have different RNA-binding activities","volume":"11","author":"Burd","year":"1991","journal-title":"Mol Cell Biol"},{"key":"10.1038\/mtna.2016.20_bib17","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1006\/jmbi.1996.0065","article-title":"Xenopus poly(A) binding protein: functional domains in RNA binding and protein-protein interaction","volume":"256","author":"K\u00fchn","year":"1996","journal-title":"J Mol Biol"},{"key":"10.1038\/mtna.2016.20_bib18","doi-asserted-by":"crossref","first-page":"7480","DOI":"10.1093\/emboj\/17.24.7480","article-title":"A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation","volume":"17","author":"Imataka","year":"1998","journal-title":"EMBO J"},{"key":"10.1038\/mtna.2016.20_bib19","doi-asserted-by":"crossref","first-page":"7168","DOI":"10.1002\/j.1460-2075.1996.tb01108.x","article-title":"Association of the yeast poly(A) tail binding protein with translation initiation factor eIF-4G","volume":"15","author":"Tarun","year":"1996","journal-title":"EMBO J"},{"key":"10.1038\/mtna.2016.20_bib20","doi-asserted-by":"crossref","first-page":"17166","DOI":"10.1016\/S0021-9258(17)32536-X","article-title":"Poly(A) binds to initiation factors and increases cap-dependent translation in vitro","volume":"269","author":"Gallie","year":"1994","journal-title":"J Biol Chem"},{"key":"10.1038\/mtna.2016.20_bib21","doi-asserted-by":"crossref","first-page":"32268","DOI":"10.1074\/jbc.M004304200","article-title":"Cap-Poly(A) synergy in mammalian cell-free extracts. Investigation of the requirements for poly(A)-mediated stimulation of translation initiation","volume":"275","author":"Michel","year":"2000","journal-title":"J Biol Chem"},{"key":"10.1038\/mtna.2016.20_bib22","doi-asserted-by":"crossref","first-page":"6259","DOI":"10.1128\/MCB.20.17.6259-6268.2000","article-title":"Eukaryotic translation initiation factor 4GI is a cellular target for NS1 protein, a translational activator of influenza virus","volume":"20","author":"Arag\u00f3n","year":"2000","journal-title":"Mol Cell Biol"},{"issue":"Pt 12","key":"10.1038\/mtna.2016.20_bib23","doi-asserted-by":"crossref","first-page":"3263","DOI":"10.1099\/vir.0.19487-0","article-title":"PABP1 and eIF4GI associate with influenza virus NS1 protein in viral mRNA translation initiation complexes","volume":"84","author":"Burgui","year":"2003","journal-title":"J Gen Virol"},{"key":"10.1038\/mtna.2016.20_bib24","doi-asserted-by":"crossref","first-page":"12427","DOI":"10.1128\/JVI.01105-07","article-title":"Influenza virus mRNA translation revisited: is the eIF4E cap-binding factor required for viral mRNA translation?","volume":"81","author":"Burgui","year":"2007","journal-title":"J Virol"},{"key":"10.1038\/mtna.2016.20_bib25","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.virol.2011.02.012","article-title":"Functional impairment of eIF4A and eIF4G factors correlates with inhibition of influenza virus mRNA translation","volume":"413","author":"Y\u00e1ng\u00fcez","year":"2011","journal-title":"Virology"},{"key":"10.1038\/mtna.2016.20_bib26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.virusres.2010.12.016","article-title":"So similar, yet so different: selective translation of capped and polyadenylated viral mRNAs in the influenza virus infected cell","volume":"156","author":"Y\u00e1ng\u00fcez","year":"2011","journal-title":"Virus Res"},{"key":"10.1038\/mtna.2016.20_bib27","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1038\/346818a0","article-title":"In vitro selection of RNA molecules that bind specific ligands","volume":"346","author":"Ellington","year":"1990","journal-title":"Nature"},{"key":"10.1038\/mtna.2016.20_bib28","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1126\/science.2200121","article-title":"Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase","volume":"249","author":"Tuerk","year":"1990","journal-title":"Science"},{"key":"10.1038\/mtna.2016.20_bib29","doi-asserted-by":"crossref","first-page":"e0140048","DOI":"10.1371\/journal.pone.0140048","article-title":"Molecular and functional characterization of ssDNA aptamers that specifically bind Leishmania infantum PABP","volume":"10","author":"Guerra-P\u00e9rez","year":"2015","journal-title":"PLoS One"},{"key":"10.1038\/mtna.2016.20_bib30","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.febslet.2010.11.042","article-title":"Identification and functional characterization of a poly(A)-binding protein from Leishmania infantum (LiPABP)","volume":"585","author":"Guerra","year":"2011","journal-title":"FEBS Lett"},{"key":"10.1038\/mtna.2016.20_bib31","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1093\/infdis\/152.5.938","article-title":"A rapid colorimetric assay of fungal viability with the tetrazolium salt MTT","volume":"152","author":"Levitz","year":"1985","journal-title":"J Infect Dis"},{"key":"10.1038\/mtna.2016.20_bib32","doi-asserted-by":"crossref","first-page":"22223","DOI":"10.1016\/S0021-9258(18)41511-6","article-title":"Translational control by influenza virus. Selective translation is mediated by sequences within the viral mRNA 5'-untranslated region","volume":"268","author":"Garfinkel","year":"1993","journal-title":"J Biol Chem"},{"key":"10.1038\/mtna.2016.20_bib33","doi-asserted-by":"crossref","first-page":"28433","DOI":"10.1074\/jbc.270.47.28433","article-title":"Translational control by influenza virus. Identification of cis-acting sequences and trans-acting factors which may regulate selective viral mRNA translation","volume":"270","author":"Park","year":"1995","journal-title":"J Biol Chem"},{"key":"10.1038\/mtna.2016.20_bib34","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.virol.2011.10.028","article-title":"Influenza virus polymerase confers independence of the cellular cap-binding factor eIF4E for viral mRNA translation","volume":"422","author":"Y\u00e1ng\u00fcez","year":"2012","journal-title":"Virology"},{"key":"10.1038\/mtna.2016.20_bib35","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/irv.12293","article-title":"Overview of the 3rd isirv-Antiviral Group Conference\u2013advances in clinical management","volume":"9","author":"Hurt","year":"2015","journal-title":"Influenza Other Respir Viruses"},{"key":"10.1038\/mtna.2016.20_bib36","doi-asserted-by":"crossref","first-page":"791","DOI":"10.2741\/s102","article-title":"Broad-spectrum and virus-specific nucleic acid-based antivirals against influenza","volume":"2","author":"Wong","year":"2010","journal-title":"Front Biosci (Schol Ed)"},{"key":"10.1038\/mtna.2016.20_bib37","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1007\/s12275-010-0120-z","article-title":"Replication and pathogenesis of the pandemic (H1N1) 2009 influenza virus in mammalian models","volume":"48","author":"Kwon","year":"2010","journal-title":"J Microbiol"},{"key":"10.1038\/mtna.2016.20_bib38","doi-asserted-by":"crossref","first-page":"48410","DOI":"10.1074\/jbc.M409059200","article-title":"A DNA aptamer prevents influenza infection by blocking the receptor binding region of the viral hemagglutinin","volume":"279","author":"Jeon","year":"2004","journal-title":"J Biol Chem"},{"key":"10.1038\/mtna.2016.20_bib39","doi-asserted-by":"crossref","first-page":"e97696","DOI":"10.1371\/journal.pone.0097696","article-title":"Designing anti-influenza aptamers: novel quantitative structure activity relationship approach gives insights into aptamer-virus interaction","volume":"9","author":"Musafia","year":"2014","journal-title":"PLoS One"},{"key":"10.1038\/mtna.2016.20_bib40","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.antiviral.2013.09.004","article-title":"Single-stranded DNA aptamer that specifically binds to the influenza virus NS1 protein suppresses interferon antagonism","volume":"100","author":"Woo","year":"2013","journal-title":"Antiviral Res"},{"key":"10.1038\/mtna.2016.20_bib41","doi-asserted-by":"crossref","first-page":"4082","DOI":"10.1128\/AAC.00306-15","article-title":"Cross-protection of influenza A virus infection by a DNA aptamer targeting the PA endonuclease domain","volume":"59","author":"Yuan","year":"2015","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1038\/mtna.2016.20_bib42","doi-asserted-by":"crossref","first-page":"2724","DOI":"10.1093\/nar\/gkr1098","article-title":"5'-Triphosphate-RNA-independent activation of RIG-I via RNA aptamer with enhanced antiviral activity","volume":"40","author":"Hwang","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"10.1038\/mtna.2016.20_bib43","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.cyto.2015.07.002","article-title":"The catcher in the RIG-I","volume":"76","author":"Weber","year":"2015","journal-title":"Cytokine"},{"key":"10.1038\/mtna.2016.20_bib44","doi-asserted-by":"crossref","first-page":"4723","DOI":"10.1093\/emboj\/19.17.4723","article-title":"Multiple portions of poly(A)-binding protein stimulate translation in vivo","volume":"19","author":"Gray","year":"2000","journal-title":"EMBO J"},{"key":"10.1038\/mtna.2016.20_bib45","doi-asserted-by":"crossref","first-page":"9046","DOI":"10.1073\/pnas.94.17.9046","article-title":"Translation initiation factor eIF4G mediates in vitro poly(A) tail-dependent translation","volume":"94","author":"Tarun","year":"1997","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/mtna.2016.20_bib46","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1016\/S0960-9822(00)00701-6","article-title":"Interaction of eIF4G with poly(A)-binding protein stimulates translation and is critical for Xenopus oocyte maturation","volume":"10","author":"Wakiyama","year":"2000","journal-title":"Curr Biol"},{"key":"10.1038\/mtna.2016.20_bib47","doi-asserted-by":"crossref","first-page":"207","DOI":"10.4161\/rna.8.2.14513","article-title":"The influenza virus RNA synthesis machine: advances in its structure and function","volume":"8","author":"Resa-Infante","year":"2011","journal-title":"RNA Biol"},{"key":"10.1038\/mtna.2016.20_bib48","first-page":"31","article-title":"Liposome based formulations for the delivery of oligonucleotides","volume":"11","author":"Fattal","year":"2001","journal-title":"STP Pharma Sciences"},{"key":"10.1038\/mtna.2016.20_bib49","doi-asserted-by":"crossref","first-page":"459","DOI":"10.3109\/10409238.2011.614592","article-title":"Chimeric aptamers in cancer cell-targeted drug delivery","volume":"46","author":"Kanwar","year":"2011","journal-title":"Crit Rev Biochem Mol Biol"},{"key":"10.1038\/mtna.2016.20_bib50","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1038\/labinvest.3700535","article-title":"A DNA aptamer population specifically detects Leishmania infantum H2A antigen","volume":"87","author":"Ramos","year":"2007","journal-title":"Lab Invest"},{"key":"10.1038\/mtna.2016.20_bib51","doi-asserted-by":"crossref","first-page":"5315","DOI":"10.1128\/JVI.02129-06","article-title":"Influenza virus infection causes specific degradation of the largest subunit of cellular RNA polymerase II","volume":"81","author":"Rodriguez","year":"2007","journal-title":"J Virol"},{"key":"10.1038\/mtna.2016.20_bib52","doi-asserted-by":"crossref","first-page":"15246","DOI":"10.1128\/JVI.79.24.15246-15257.2005","article-title":"Genetic analysis of influenza virus NS1 gene: a temperature-sensitive mutant shows defective formation of virus particles","volume":"79","author":"Garaigorta","year":"2005","journal-title":"J Virol"},{"key":"10.1038\/mtna.2016.20_bib53","doi-asserted-by":"crossref","first-page":"1905","DOI":"10.1046\/j.1471-4159.1998.71051905.x","article-title":"Increased activity of eukaryotic initiation factor 2B in PC12 cells in response to differentiation by nerve growth factor","volume":"71","author":"Mu\u00f1oz","year":"1998","journal-title":"J Neurochem"},{"key":"10.1038\/mtna.2016.20_bib54","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jviromet.2010.12.014","article-title":"Strand-specific real-time RT-PCR for distinguishing influenza vRNA, cRNA, and mRNA","volume":"173","author":"Kawakami","year":"2011","journal-title":"J Virol Methods"}],"container-title":["Molecular Therapy - Nucleic Acids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2162253117300343?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2162253117300343?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T01:24:18Z","timestamp":1761873858000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2162253117300343"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":54,"alternative-id":["S2162253117300343"],"URL":"https:\/\/doi.org\/10.1038\/mtna.2016.20","relation":{},"ISSN":["2162-2531"],"issn-type":[{"value":"2162-2531","type":"print"}],"subject":[],"published":{"date-parts":[[2016]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Inhibition of Influenza Virus Replication by DNA Aptamers Targeting a Cellular Component of Translation Initiation","name":"articletitle","label":"Article Title"},{"value":"Molecular Therapy - Nucleic Acids","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1038\/mtna.2016.20","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2016 Official journal of the American Society of Gene & Cell Therapy. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"e308"}}