{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:11:41Z","timestamp":1790514701265,"version":"4.1.0"},"reference-count":55,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2017,1,25]],"date-time":"2017-01-25T00:00:00Z","timestamp":1485302400000},"content-version":"vor","delay-in-days":390,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Therapy - Nucleic Acids"],"published-print":{"date-parts":[[2016]]},"DOI":"10.1038\/mtna.2016.74","type":"journal-article","created":{"date-parts":[[2016,9,27]],"date-time":"2016-09-27T11:25:08Z","timestamp":1474975508000},"page":"e367","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"special_numbering":"C","title":["Ecdysone Receptor-based Singular Gene Switches for Regulated Transgene Expression in Cells and Adult Rodent Tissues"],"prefix":"10.1016","volume":"5","author":[{"given":"Seoghyun","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung-Cheol","family":"Sohn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dae-Kyoung","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minho","family":"Won","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyeong Ah","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung-Kyu","family":"Ju","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kidong","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young-Ki","family":"Bae","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang Min","family":"Hur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyunju","family":"Ro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1038\/mtna.2016.74_bib1","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/S0958-1669(98)80028-1","article-title":"Recent advances in inducible gene expression systems","volume":"9","author":"Rossi","year":"1998","journal-title":"Curr Opin Biotechnol"},{"key":"10.1038\/mtna.2016.74_bib2","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1038\/sj.gt.3301120","article-title":"Regulated gene expression systems","volume":"7","author":"Clackson","year":"2000","journal-title":"Gene Ther"},{"key":"10.1038\/mtna.2016.74_bib3","series-title":"Viral Gene Therapy","first-page":"285","article-title":"Gene regulatable lentiviral system","author":"Suzuki","year":"2011"},{"key":"10.1038\/mtna.2016.74_bib4","doi-asserted-by":"crossref","first-page":"5547","DOI":"10.1073\/pnas.89.12.5547","article-title":"Tight control of gene expression in mammalian cells by tetracycline-responsive promoters","volume":"89","author":"Gossen","year":"1992","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/mtna.2016.74_bib5","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/S0076-6879(00)27292-3","article-title":"Tet repressor-based system for regulated gene expression in eukaryotic cells: principles and advances","volume":"327","author":"Baron","year":"2000","journal-title":"Methods Enzymol"},{"key":"10.1038\/mtna.2016.74_bib6","doi-asserted-by":"crossref","first-page":"3109","DOI":"10.1046\/j.1432-1033.2003.03694.x","article-title":"Gene regulation by tetracyclines. Constraints of resistance regulation in bacteria shape TetR for application in eukaryotes","volume":"270","author":"Berens","year":"2003","journal-title":"Eur J Biochem"},{"key":"10.1038\/mtna.2016.74_bib7","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1002\/biot.201500236","article-title":"Selecting the optimal Tet-On system for doxycycline-inducible gene expression in transiently transfected and stably transduced mammalian cells","volume":"11","author":"Das","year":"2016","journal-title":"Biotechnol J"},{"key":"10.1038\/mtna.2016.74_bib8","doi-asserted-by":"crossref","first-page":"45168","DOI":"10.1074\/jbc.M106924200","article-title":"Tetracycline-regulated gene expression mediated by a novel chimeric repressor that recruits histone deacetylases in mammalian cells","volume":"276","author":"Jiang","year":"2001","journal-title":"J Biol Chem"},{"key":"10.1038\/mtna.2016.74_bib9","doi-asserted-by":"crossref","first-page":"19933","DOI":"10.1073\/pnas.1007799107","article-title":"Dually inducible TetON systems for tissue-specific conditional gene expression in zebrafish","volume":"107","author":"Knopf","year":"2010","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/mtna.2016.74_bib10","doi-asserted-by":"crossref","first-page":"390","DOI":"10.2144\/04363BM03","article-title":"Dual regulation of gene expression mediated by tetracycline and Cre recombinase","volume":"36","author":"Park","year":"2004","journal-title":"Biotechniques"},{"key":"10.1038\/mtna.2016.74_bib11","doi-asserted-by":"crossref","first-page":"874","DOI":"10.1038\/gt.2009.46","article-title":"Independent and high-level dual-gene expression in adult stem-progenitor cells from a single lentiviral vector","volume":"16","author":"Tian","year":"2009","journal-title":"Gene Ther"},{"key":"10.1038\/mtna.2016.74_bib12","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.tig.2005.04.009","article-title":"Transcriptional interference\u2013a crash course","volume":"21","author":"Shearwin","year":"2005","journal-title":"Trends Genet"},{"key":"10.1038\/mtna.2016.74_bib13","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1038\/366476a0","article-title":"Functional ecdysone receptor is the product of EcR and Ultraspiracle genes","volume":"366","author":"Yao","year":"1993","journal-title":"Nature"},{"key":"10.1038\/mtna.2016.74_bib14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0083-6729(00)60016-X","article-title":"Ecdysone receptors and their biological actions","volume":"60","author":"Riddiford","year":"2000","journal-title":"Vitam Horm"},{"key":"10.1038\/mtna.2016.74_bib15","doi-asserted-by":"crossref","first-page":"14512","DOI":"10.1073\/pnas.260499497","article-title":"Identification of ligands and coligands for the ecdysone-regulated gene switch","volume":"97","author":"Saez","year":"2000","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/mtna.2016.74_bib16","doi-asserted-by":"crossref","first-page":"191","DOI":"10.2144\/05392ST01","article-title":"Inducible control of transgene expression with ecdysone receptor: gene switches with high sensitivity, robust expression, and reduced size","volume":"39","author":"Karzenowski","year":"2005","journal-title":"Biotechniques"},{"key":"10.1038\/mtna.2016.74_bib17","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1038\/nchembio858","article-title":"Small-molecule regulation of zebrafish gene expression","volume":"3","author":"Esengil","year":"2007","journal-title":"Nat Chem Biol"},{"key":"10.1038\/mtna.2016.74_bib18","doi-asserted-by":"crossref","first-page":"433","DOI":"10.2144\/000113121","article-title":"Cell cycle quiescence can suppress transcription from an ecdysone receptor-based inducible promoter in mammalian cells","volume":"46","author":"Wallbaum","year":"2009","journal-title":"Biotechniques"},{"key":"10.1038\/mtna.2016.74_bib19","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1023\/A:1022113817892","article-title":"Chemical-inducible, ecdysone receptor-based gene expression system for plants","volume":"12","author":"Padidam","year":"2003","journal-title":"Transgenic Res"},{"key":"10.1038\/mtna.2016.74_bib20","doi-asserted-by":"crossref","first-page":"354","DOI":"10.2144\/000112536","article-title":"In-fusion assembly: seamless engineering of multidomain fusion proteins, modular vectors, and mutations","volume":"43","author":"Zhu","year":"2007","journal-title":"Biotechniques"},{"key":"10.1038\/mtna.2016.74_bib21","doi-asserted-by":"crossref","first-page":"5440","DOI":"10.1128\/AEM.00844-12","article-title":"One-step sequence- and ligation-independent cloning as a rapid and versatile cloning method for functional genomics studies","volume":"78","author":"Jeong","year":"2012","journal-title":"Appl Environ Microbiol"},{"key":"10.1038\/mtna.2016.74_bib22","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1534\/genetics.106.060244","article-title":"Functional dissection of the Tol2 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition","volume":"174","author":"Urasaki","year":"2006","journal-title":"Genetics"},{"key":"10.1038\/mtna.2016.74_bib23","doi-asserted-by":"crossref","first-page":"331","DOI":"10.2144\/000112909","article-title":"Real-time imaging of mitochondria in transgenic zebrafish expressing mitochondrially targeted GFP","volume":"45","author":"Kim","year":"2008","journal-title":"Biotechniques"},{"key":"10.1038\/mtna.2016.74_bib24","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1091\/mbc.e11-09-0817","article-title":"The Ankrd 13 family of UIM-bearing proteins regulates EGF receptor endocytosis from the plasma membrane","volume":"23","author":"Tanno","year":"2012","journal-title":"Mol Biol Cell"},{"key":"10.1038\/mtna.2016.74_bib25","doi-asserted-by":"crossref","first-page":"e61099","DOI":"10.1371\/journal.pone.0061099","article-title":"miR-204 targeting of Ankrd13A controls both mesenchymal neural crest and lens cell migration","volume":"8","author":"Avellino","year":"2013","journal-title":"PLoS One"},{"key":"10.1038\/mtna.2016.74_bib26","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1089\/hum.2004.15.1022","article-title":"Biology of adenovirus and its use as a vector for gene therapy","volume":"15","author":"McConnell","year":"2004","journal-title":"Hum Gene Ther"},{"key":"10.1038\/mtna.2016.74_bib27","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1146\/annurev-bioeng-071813-104938","article-title":"Viral vectors for gene therapy: translational and clinical outlook","volume":"17","author":"Kotterman","year":"2015","journal-title":"Annu Rev Biomed Eng"},{"key":"10.1038\/mtna.2016.74_bib28","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1099\/0022-1317-40-1-45","article-title":"Infection of mouse liver by human adenovirus type 5","volume":"40","author":"Duncan","year":"1978","journal-title":"J Gen Virol"},{"key":"10.1038\/mtna.2016.74_bib29","doi-asserted-by":"crossref","first-page":"8180","DOI":"10.1073\/pnas.91.17.8180","article-title":"A regulatory system for use in gene transfer","volume":"91","author":"Wang","year":"1994","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/mtna.2016.74_bib30","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1038\/nm0996-1028","article-title":"A humanized system for pharmacologic control of gene expression","volume":"2","author":"Rivera","year":"1996","journal-title":"Nat Med"},{"key":"10.1038\/mtna.2016.74_bib31","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1073\/pnas.96.2.355","article-title":"Adenovirus-mediated regulable target gene expression in vivo","volume":"96","author":"Burcin","year":"1999","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/mtna.2016.74_bib32","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/S0378-1119(03)00506-7","article-title":"Regulated gene expression from adenovirus vectors: a systematic comparison of various inducible systems","volume":"309","author":"Xu","year":"2003","journal-title":"Gene"},{"key":"10.1038\/mtna.2016.74_bib33","doi-asserted-by":"crossref","first-page":"107","DOI":"10.4172\/2157-7412.1000107","article-title":"Gene switches for deliberate regulation of transgene expression: recent advances in system development and uses","volume":"2","author":"Vilaboa","year":"2011","journal-title":"J Genet Syndr Gene Ther"},{"key":"10.1038\/mtna.2016.74_bib34","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.1038\/sj.mt.6300171","article-title":"Development and validation of a robust and versatile one-plasmid regulated gene expression system","volume":"15","author":"Szymanski","year":"2007","journal-title":"Mol Ther"},{"key":"10.1038\/mtna.2016.74_bib35","doi-asserted-by":"crossref","first-page":"e106","DOI":"10.1038\/mtna.2013.35","article-title":"Adeno-associated virus-mediated, mifepristone-regulated transgene expression in the brain","volume":"2","author":"Maddalena","year":"2013","journal-title":"Mol Ther Nucleic Acids"},{"key":"10.1038\/mtna.2016.74_bib36","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1111\/j.1749-6632.1995.tb31382.x","article-title":"Clinical uses of mifepristone (MFP)","volume":"761","author":"Ulmann","year":"1995","journal-title":"Ann NY Acad Sci"},{"key":"10.1038\/mtna.2016.74_bib37","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/2001-1326-1-29","article-title":"Rapamycin and mTOR: a serendipitous discovery and implications for breast cancer","volume":"1","author":"Seto","year":"2012","journal-title":"Clin Transl Med"},{"key":"10.1038\/mtna.2016.74_bib38","doi-asserted-by":"crossref","first-page":"13796","DOI":"10.1073\/pnas.241184198","article-title":"FRAP\/mTOR is required for proliferation and patterning during embryonic development in the mouse","volume":"98","author":"Hentges","year":"2001","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/mtna.2016.74_bib39","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/S0010-7824(03)00171-9","article-title":"Pharmacological properties of mifepristone: toxicology and safety in animal and human studies","volume":"68","author":"Sitruk-Ware","year":"2003","journal-title":"Contraception"},{"key":"10.1038\/mtna.2016.74_bib40","doi-asserted-by":"crossref","first-page":"4715","DOI":"10.1021\/ja043277y","article-title":"Characterization of the FKBP. rapamycin. FRB ternary complex","volume":"127","author":"Banaszynski","year":"2005","journal-title":"J Am Chem Soc"},{"key":"10.1038\/mtna.2016.74_bib41","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1016\/j.febslet.2012.04.061","article-title":"Protein translocation as a tool: The current rapamycin story","volume":"586","author":"Putyrski","year":"2012","journal-title":"FEBS Lett"},{"key":"10.1038\/mtna.2016.74_bib42","doi-asserted-by":"crossref","first-page":"3346","DOI":"10.1073\/pnas.93.8.3346","article-title":"Ecdysone-inducible gene expression in mammalian cells and transgenic mice","volume":"93","author":"No","year":"1996","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1038\/mtna.2016.74_bib43","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1002\/ps.604","article-title":"Synthesis and insecticidal activity of benzoheterocyclic analogues of N'-benzoyl-N-(tert-butyl)benzohydrazide: Part 1. Design of benzoheterocyclic analogues","volume":"59","author":"Sawada","year":"2003","journal-title":"Pest Manag Sci"},{"key":"10.1038\/mtna.2016.74_bib44","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1016\/0006-2952(88)90529-1","article-title":"Excretion and metabolism of injected ecdysone in the white mouse","volume":"37","author":"Lafont","year":"1988","journal-title":"Biochem Pharmacol"},{"key":"10.1038\/mtna.2016.74_bib45","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1002\/1526-4998(200102)57:2<115::AID-PS245>3.0.CO;2-A","article-title":"The chemical and biological properties of methoxyfenozide, a new insecticidal ecdysteroid agonist","volume":"57","author":"Carlson","year":"2001","journal-title":"Pest Manag Sci"},{"key":"10.1038\/mtna.2016.74_bib46","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1006\/mthe.1999.0023","article-title":"Adenovirus-mediated inducible gene expression in vivo by a hybrid ecdysone receptor","volume":"1","author":"Hoppe","year":"2000","journal-title":"Mol Ther"},{"key":"10.1038\/mtna.2016.74_bib47","doi-asserted-by":"crossref","first-page":"a015149","DOI":"10.1101\/cshperspect.a015149","article-title":"Gene therapy for skin diseases","volume":"4","author":"Gorell","year":"2014","journal-title":"Cold Spring Harb Perspect Med"},{"key":"10.1038\/mtna.2016.74_bib48","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/S0022-4804(03)00023-4","article-title":"Insulin gene transfer with adenovirus vector via the spleen safely and effectively improves posthepatectomized conditions in diabetic rats","volume":"110","author":"Matsumoto","year":"2003","journal-title":"J Surg Res"},{"key":"10.1038\/mtna.2016.74_bib49","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1038\/mt.2010.255","article-title":"Remission of diabetes by insulin gene therapy using a hepatocyte-specific and glucose-responsive synthetic promoter","volume":"19","author":"Han","year":"2011","journal-title":"Mol Ther"},{"key":"10.1038\/mtna.2016.74_bib50","doi-asserted-by":"crossref","first-page":"199","DOI":"10.2119\/2006-00031.Hirsch","article-title":"Adenoviral gene delivery to primary human cutaneous cells and burn wounds","volume":"12","author":"Hirsch","year":"2006","journal-title":"Mol Med"},{"key":"10.1038\/mtna.2016.74_bib51","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.ejcb.2011.01.006","article-title":"New insights into stability of recombinant adenovirus vector genomes in mammalian cells","volume":"91","author":"Rauschhuber","year":"2012","journal-title":"Eur J Cell Biol"},{"key":"10.1038\/mtna.2016.74_bib52","doi-asserted-by":"crossref","first-page":"e83","DOI":"10.1038\/mtna.2013.11","article-title":"A novel adenoviral hybrid-vector system carrying a plasmid replicon for safe and efficient cell and gene therapeutic applications","volume":"2","author":"Voigtlander","year":"2013","journal-title":"Mol Ther Nucleic Acids"},{"key":"10.1038\/mtna.2016.74_bib53","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1016\/j.bbrc.2007.03.065","article-title":"Effect of thioredoxin reductase 1 on glucocorticoid receptor activity in human outer root sheath cells","volume":"356","author":"Sohn","year":"2007","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1038\/mtna.2016.74_bib54","doi-asserted-by":"crossref","first-page":"e13216","DOI":"10.1371\/journal.pone.0013216","article-title":"Brn2 is a transcription factor regulating keratinocyte differentiation with a possible role in the pathogenesis of lichen planus","volume":"5","author":"Shi","year":"2010","journal-title":"PLoS One"},{"key":"10.1038\/mtna.2016.74_bib55","doi-asserted-by":"crossref","first-page":"652","DOI":"10.2478\/s11658-011-0029-5","article-title":"Real-time PCR for the detection of precise transgene copy number in durum wheat","volume":"16","author":"Gadaleta","year":"2011","journal-title":"Cell Mol Biol Lett"}],"container-title":["Molecular Therapy - Nucleic Acids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2162253117300926?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2162253117300926?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T01:26:08Z","timestamp":1761873968000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2162253117300926"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":55,"alternative-id":["S2162253117300926"],"URL":"https:\/\/doi.org\/10.1038\/mtna.2016.74","relation":{},"ISSN":["2162-2531"],"issn-type":[{"value":"2162-2531","type":"print"}],"subject":[],"published":{"date-parts":[[2016]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Ecdysone Receptor-based Singular Gene Switches for Regulated Transgene Expression in Cells and Adult Rodent Tissues","name":"articletitle","label":"Article Title"},{"value":"Molecular Therapy - Nucleic Acids","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1038\/mtna.2016.74","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2016 Official journal of the American Society of Gene & Cell Therapy. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"e367"}}