{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T03:33:52Z","timestamp":1791171232551,"version":"4.1.0"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"7207","license":[{"start":{"date-parts":[[2008,7,23]],"date-time":"2008-07-23T00:00:00Z","timestamp":1216771200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nature"],"published-print":{"date-parts":[[2008,8]]},"DOI":"10.1038\/nature07160","type":"journal-article","created":{"date-parts":[[2008,7,23]],"date-time":"2008-07-23T14:35:30Z","timestamp":1216823730000},"page":"1009-1013","source":"Crossref","is-referenced-by-count":97,"title":["Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspFU"],"prefix":"10.1038","volume":"454","author":[{"given":"Hui-Chun","family":"Cheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian M.","family":"Skehan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth G.","family":"Campellone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Leong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael K.","family":"Rosen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2008,7,23]]},"reference":[{"key":"BFnature07160_CR1","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/j.devcel.2004.07.004","volume":"7","author":"KG Campellone","year":"2004","unstructured":"Campellone, K. G., Robbins, D. & Leong, J. M. EspFU is a translocated EHEC effector that interacts with Tir and N-WASP and promotes Nck-independent actin assembly. Dev. Cell 7, 217\u2013228 (2004)","journal-title":"Dev. Cell"},{"key":"BFnature07160_CR2","doi-asserted-by":"publisher","first-page":"1167","DOI":"10.1111\/j.1462-5822.2004.00459.x","volume":"6","author":"J Garmendia","year":"2004","unstructured":"Garmendia, J. et al. TccP is an enterohaemorrhagic Escherichia coli O157:H7 type III effector protein that couples Tir to the actin-cytoskeleton. Cell. Microbiol. 6, 1167\u20131183 (2004)","journal-title":"Cell. Microbiol."},{"key":"BFnature07160_CR3","doi-asserted-by":"publisher","first-page":"1488","DOI":"10.1111\/j.1462-5822.2006.00728.x","volume":"8","author":"KG Campellone","year":"2006","unstructured":"Campellone, K. G. et al. Enterohaemorrhagic Escherichia coli Tir requires a C-terminal 12-residue peptide to initiate EspFu-mediated actin assembly and harbours N-terminal sequences that influence pedestal length. Cell. Microbiol. 8, 1488\u20131503 (2006)","journal-title":"Cell. Microbiol."},{"key":"BFnature07160_CR4","doi-asserted-by":"publisher","first-page":"1444","DOI":"10.1111\/j.1462-5822.2006.00723.x","volume":"8","author":"J Garmendia","year":"2006","unstructured":"Garmendia, J., Carlier, M. F., Egile, C., Didry, D. & Frankel, G. Characterization of TccP-mediated N-WASP activation during enterohaemorrhagic Escherichia coli infection. Cell. Microbiol. 8, 1444\u20131455 (2006)","journal-title":"Cell. Microbiol."},{"key":"BFnature07160_CR5","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1111\/j.1462-5822.2004.00364.x","volume":"6","author":"S Lommel","year":"2004","unstructured":"Lommel, S., Benesch, S., Rohde, M., Wehland, J. & Rottner, K. Enterohaemorrhagic and enteropathogenic Escherichia coli use different mechanisms for actin pedestal formation that converge on N-WASP. Cell. Microbiol. 6, 243\u2013254 (2004)","journal-title":"Cell. Microbiol."},{"key":"BFnature07160_CR6","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1016\/j.jmb.2004.02.036","volume":"338","author":"M Buck","year":"2004","unstructured":"Buck, M., Xu, W. & Rosen, M. K. A two-state allosteric model for autoinhibition rationalizes WASP signal integration and targeting. J. Mol. Biol. 338, 271\u2013285 (2004)","journal-title":"J. Mol. Biol."},{"key":"BFnature07160_CR7","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1038\/35004513","volume":"404","author":"AS Kim","year":"2000","unstructured":"Kim, A. S., Kakalis, L. T., Abdul-Manan, N., Liu, G. A. & Rosen, M. K. Autoinhibition and activation mechanisms of the Wiskott\u2013Aldrich syndrome protein. Nature 404, 151\u2013158 (2000)","journal-title":"Nature"},{"key":"BFnature07160_CR8","doi-asserted-by":"publisher","first-page":"1215","DOI":"10.1016\/S1097-2765(03)00139-4","volume":"11","author":"E Torres","year":"2003","unstructured":"Torres, E. & Rosen, M. K. Contingent phosphorylation\/dephosphorylation provides a mechanism of molecular memory in WASP. Mol. Cell 11, 1215\u20131227 (2003)","journal-title":"Mol. Cell"},{"key":"BFnature07160_CR9","doi-asserted-by":"crossref","first-page":"re5","DOI":"10.1126\/stke.3352006re5","volume":"2006","author":"S Munter","year":"2006","unstructured":"Munter, S., Way, M. & Frischknecht, F. Signaling during pathogen infection. Sci. STKE 2006, re5 (2006)","journal-title":"Sci. STKE"},{"key":"BFnature07160_CR10","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1016\/j.mib.2005.12.008","volume":"9","author":"E Caron","year":"2006","unstructured":"Caron, E. et al. Subversion of actin dynamics by EPEC and EHEC. Curr. Opin. Microbiol. 9, 40\u201345 (2006)","journal-title":"Curr. Opin. Microbiol."},{"key":"BFnature07160_CR11","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1038\/nrmicro1391","volume":"4","author":"RD Hayward","year":"2006","unstructured":"Hayward, R. D., Leong, J. M., Koronakis, V. & Campellone, K. G. Exploiting pathogenic Escherichia coli to model transmembrane receptor signalling. Nature Rev. Microbiol. 4, 358\u2013370 (2006)","journal-title":"Nature Rev. Microbiol."},{"key":"BFnature07160_CR12","doi-asserted-by":"publisher","first-page":"775","DOI":"10.1038\/nature01603","volume":"422","author":"S Gruenheid","year":"2003","unstructured":"Gruenheid, S. & Finlay, B. B. Microbial pathogenesis and cytoskeletal function. Nature 422, 775\u2013781 (2003)","journal-title":"Nature"},{"key":"BFnature07160_CR13","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.mib.2004.12.015","volume":"8","author":"JE Gal\u00e1n","year":"2005","unstructured":"Gal\u00e1n, J. E. & Cossart, P. Host-pathogen interactions: a diversity of themes, a variety of molecular machines. Curr. Opin. Microbiol. 8, 1\u20133 (2005)","journal-title":"Curr. Opin. Microbiol."},{"key":"BFnature07160_CR14","doi-asserted-by":"publisher","first-page":"603","DOI":"10.3201\/eid1104.040739","volume":"11","author":"JM Rangel","year":"2005","unstructured":"Rangel, J. M., Sparling, P. H., Crowe, C., Griffin, P. M. & Swerdlow, D. L. Epidemiology of Escherichia coli O157:H7 outbreaks, United States, 1982\u20132002. Emerg. Infect. Dis. 11, 603\u2013609 (2005)","journal-title":"Emerg. Infect. Dis."},{"key":"BFnature07160_CR15","doi-asserted-by":"crossref","first-page":"2389","DOI":"10.1128\/IAI.67.5.2389-2398.1999","volume":"67","author":"R DeVinney","year":"1999","unstructured":"DeVinney, R. et al. Enterohemorrhagic Escherichia coli O157:H7 produces Tir, which is translocated to the host cell membrane but is not tyrosine phosphorylated. Infect. Immun. 67, 2389\u20132398 (1999)","journal-title":"Infect. Immun."},{"key":"BFnature07160_CR16","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1146\/annurev.biochem.70.1.649","volume":"70","author":"HN Higgs","year":"2001","unstructured":"Higgs, H. N. & Pollard, T. D. Regulation of actin filament network formation through Arp2\/3 complex: Activation by a diverse array of proteins. Annu. Rev. Biochem. 70, 649\u2013676 (2001)","journal-title":"Annu. Rev. Biochem."},{"key":"BFnature07160_CR17","doi-asserted-by":"publisher","first-page":"10589","DOI":"10.1074\/jbc.M507470200","volume":"281","author":"AE Kelly","year":"2006","unstructured":"Kelly, A. E., Kranitz, H., Dotsch, V. & Mullins, R. D. Actin binding to the central domain of WASP\/Scar proteins plays a critical role in the activation of the Arp2\/3 complex. J. Biol. Chem. 281, 10589\u201310597 (2006)","journal-title":"J. Biol. Chem."},{"key":"BFnature07160_CR18","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1038\/nsb952","volume":"10","author":"SC Panchal","year":"2003","unstructured":"Panchal, S. C., Kaiser, D. A., Torres, E., Pollard, T. D. & Rosen, M. K. A conserved amphipathic helix in WASP\/Scar proteins is essential for activation of Arp2\/3 complex. Nature Struct. Biol. 10, 591\u2013598 (2003)","journal-title":"Nature Struct. Biol."},{"key":"BFnature07160_CR19","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/S0092-8674(00)80732-1","volume":"97","author":"R Rohatgi","year":"1999","unstructured":"Rohatgi, R. et al. The interaction between N-WASP and the Arp2\/3 complex links Cdc42-dependent signals to actin assembly. Cell 97, 221\u2013231 (1999)","journal-title":"Cell"},{"key":"BFnature07160_CR20","doi-asserted-by":"publisher","first-page":"14115","DOI":"10.1021\/bi0157215","volume":"40","author":"M Buck","year":"2001","unstructured":"Buck, M., Xu, W. & Rosen, M. K. Global disruption of the WASP autoinhibited structure on Cdc42 binding. Ligand displacement as a novel method for monitoring amide hydrogen exchange. Biochemistry 40, 14115\u201314122 (2001)","journal-title":"Biochemistry"},{"key":"BFnature07160_CR21","doi-asserted-by":"publisher","first-page":"5685","DOI":"10.1073\/pnas.0406472102","volume":"102","author":"DW Leung","year":"2005","unstructured":"Leung, D. W. & Rosen, M. K. The nucleotide switch in Cdc42 modulates coupling between the GTPase-binding and allosteric equilibria of Wiskott\u2013Aldrich syndrome protein. Proc. Natl Acad. Sci. USA 102, 5685\u20135690 (2005)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature07160_CR22","doi-asserted-by":"publisher","first-page":"5715","DOI":"10.1128\/JCM.43.11.5715-5720.2005","volume":"43","author":"J Garmendia","year":"2005","unstructured":"Garmendia, J. et al. Distribution of tccP in clinical enterohemorrhagic and enteropathogenic Escherichia coli isolates. J. Clin. Microbiol. 43, 5715\u20135720 (2005)","journal-title":"J. Clin. Microbiol."},{"key":"BFnature07160_CR23","doi-asserted-by":"publisher","first-page":"1265","DOI":"10.1083\/jcb.200705021","volume":"178","author":"NM Alto","year":"2007","unstructured":"Alto, N. M. et al. The type III effector EspF coordinates membrane trafficking by the spatiotemporal activation of two eukaryotic signaling pathways. J. Cell Biol. 178, 1265\u20131278 (2007)","journal-title":"J. Cell Biol."},{"key":"BFnature07160_CR24","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1016\/S0092-8674(02)01076-0","volume":"111","author":"BF Volkman","year":"2002","unstructured":"Volkman, B. F., Prehoda, K. E., Scott, J. A., Peterson, F. C. & Lim, W. A. Structure of the N-WASP EVH1 domain-WIP complex: insight into the molecular basis of Wiskott\u2013Aldrich Syndrome. Cell 111, 565\u2013576 (2002)","journal-title":"Cell"},{"key":"BFnature07160_CR25","doi-asserted-by":"publisher","first-page":"15212","DOI":"10.1021\/bi991843+","volume":"38","author":"HN Higgs","year":"1999","unstructured":"Higgs, H. N., Blanchoin, L. & Pollard, T. D. Influence of the C terminus of Wiskott-Aldrich syndrome protein (WASp) and the Arp2\/3 complex on actin polymerization. Biochemistry 38, 15212\u201315222 (1999)","journal-title":"Biochemistry"},{"key":"BFnature07160_CR26","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/0076-6879(82)85021-0","volume":"85","author":"JA Cooper","year":"1982","unstructured":"Cooper, J. A. & Pollard, T. D. Methods to measure actin polymerization. Methods Enzymol. 85, 182\u2013210 (1982)","journal-title":"Methods Enzymol."},{"key":"BFnature07160_CR27","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/S0076-6879(06)06021-6","volume":"406","author":"DW Leung","year":"2006","unstructured":"Leung, D. W., Morgan, D. M. & Rosen, M. K. Biochemical properties and inhibitors of (N-)WASP. Methods Enzymol. 406, 281\u2013296 (2006)","journal-title":"Methods Enzymol."},{"key":"BFnature07160_CR28","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/0167-7799(90)90146-O","volume":"8","author":"CN Pace","year":"1990","unstructured":"Pace, C. N. Measuring and increasing protein stability. Trends Biotechnol. 8, 93\u201398 (1990)","journal-title":"Trends Biotechnol."},{"key":"BFnature07160_CR29","doi-asserted-by":"publisher","first-page":"1227","DOI":"10.1046\/j.1365-2958.2002.02817.x","volume":"43","author":"KG Campellone","year":"2002","unstructured":"Campellone, K. G., Giese, A., Tipper, D. J. & Leong, J. M. A tyrosine-phosphorylated 12-amino-acid sequence of enteropathogenic Escherichia coli Tir binds the host adaptor protein Nck and is required for Nck localization to actin pedestals. Mol. Microbiol. 43, 1227\u20131241 (2002)","journal-title":"Mol. Microbiol."},{"key":"BFnature07160_CR30","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1093\/bioinformatics\/btl589","volume":"23","author":"W Rieping","year":"2007","unstructured":"Rieping, W. et al. ARIA2: automated NOE assignment and data integration in NMR structure calculation. Bioinformatics 23, 381\u2013382 (2007)","journal-title":"Bioinformatics"},{"key":"BFnature07160_CR31","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1023\/A:1008393201236","volume":"13","author":"NK Goto","year":"1999","unstructured":"Goto, N. K., Gardner, K. H., Mueller, G. A., Willis, R. C. & Kay, L. E. A robust and cost-effective method for the production of Val, Leu, Ile (\u03b41) methyl-protonated 15N-, 13C-, 2H-labeled proteins. J. Biomol. NMR 13, 369\u2013374 (1999)","journal-title":"J. Biomol. NMR"},{"key":"BFnature07160_CR32","doi-asserted-by":"publisher","first-page":"7599","DOI":"10.1021\/ja9706514","volume":"119","author":"KH Gardner","year":"1997","unstructured":"Gardner, K. H. & Kay, L. E. Production and incorporation of 15N, 13C, 2H (1H-\u03b41 methyl) isoleucine into proteins for multidimensional NMR studies. J. Am. Chem. Soc. 119, 7599\u20137600 (1997)","journal-title":"J. Am. Chem. Soc."},{"key":"BFnature07160_CR33","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/S0076-6879(94)39013-4","volume":"239","author":"GM Clore","year":"1994","unstructured":"Clore, G. M. & Gronenborn, A. M. Multidimensional heteronuclear nuclear magnetic resonance of proteins. Methods Enzymol. 239, 349\u2013363 (1994)","journal-title":"Methods Enzymol."},{"key":"BFnature07160_CR34","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1006\/jmrb.1994.1032","volume":"103","author":"DR Muhandiram","year":"1994","unstructured":"Muhandiram, D. R. & Kay, L. E. Gradient-enhanced triple-resonance three-dimensional NMR experiments with improved sensitivity. J. Magn. Reson. B 103, 203\u2013216 (1994)","journal-title":"J. Magn. Reson. B"},{"key":"BFnature07160_CR35","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1021\/bi00215a002","volume":"30","author":"GM Clore","year":"1991","unstructured":"Clore, G. M., Kay, L. E., Bax, A. & Gronenborn, A. M. Four-dimensional 13C\/13C-edited nuclear Overhauser enhancement spectroscopy of a protein in solution: application to interleukin 1\u03b2. Biochemistry 30, 12\u201318 (1991)","journal-title":"Biochemistry"},{"key":"BFnature07160_CR36","doi-asserted-by":"publisher","first-page":"6711","DOI":"10.1021\/ja970224q","volume":"119","author":"C Zwahlen","year":"1997","unstructured":"Zwahlen, C. et al. Methods for measurement of intermolecular NOEs by multinuclear NMR spectroscopy: application to a bacteriophage \u03bb N-peptide\/boxB RNA complex. J. Am. Chem. Soc. 119, 6711\u20136721 (1997)","journal-title":"J. Am. Chem. Soc."},{"key":"BFnature07160_CR37","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1023\/A:1008392405740","volume":"13","author":"G Cornilescu","year":"1999","unstructured":"Cornilescu, G., Delaglio, F. & Bax, A. Protein backbone angle restraints from searching a database for chemical shift and sequence homology. J. Biomol. NMR 13, 289\u2013302 (1999)","journal-title":"J. Biomol. NMR"},{"key":"BFnature07160_CR38","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1002\/prot.10299","volume":"50","author":"JP Linge","year":"2003","unstructured":"Linge, J. P., Williams, M. A., Spronk, C. A., Bonvin, A. M. & Nilges, M. Refinement of protein structures in explicit solvent. Proteins 50, 496\u2013506 (2003)","journal-title":"Proteins"},{"key":"BFnature07160_CR39","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1007\/BF00197809","volume":"6","author":"F Delaglio","year":"1995","unstructured":"Delaglio, F. et al. NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J. Biomol. NMR 6, 277\u2013293 (1995)","journal-title":"J. Biomol. NMR"},{"key":"BFnature07160_CR40","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1007\/BF00404272","volume":"4","author":"BA Johnson","year":"1994","unstructured":"Johnson, B. A. & Blevins, R. A. NMR View: A computer program for the visualization and analysis of NMR data. J. Biomol. NMR 4, 603\u2013614 (1994)","journal-title":"J. Biomol. NMR"},{"key":"BFnature07160_CR41","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1007\/BF00228148","volume":"8","author":"RA Laskowski","year":"1996","unstructured":"Laskowski, R. A., Rullmannn, J. A., MacArthur, M. W., Kaptein, R. & Thornton, J. M. AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR. J. Biomol. NMR 8, 477\u2013486 (1996)","journal-title":"J. Biomol. NMR"},{"key":"BFnature07160_CR42","first-page":"493","volume-title":"Methods in Enzymology","author":"M Carson","year":"1997","unstructured":"Carson, M., Charles, W. C. & Robert, M. S. Methods in Enzymology 493\u2013502 (Academic, 1997)"},{"key":"BFnature07160_CR43","volume-title":"The PyMOL User\u2019s Manual","author":"WL DeLano","year":"2002","unstructured":"DeLano, W. L. The PyMOL User\u2019s Manual (DeLano Scientific, 2002)"}],"container-title":["Nature"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nature07160.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nature07160","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nature07160.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T05:07:44Z","timestamp":1684472864000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nature07160"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7,23]]},"references-count":43,"journal-issue":{"issue":"7207","published-print":{"date-parts":[[2008,8]]}},"alternative-id":["BFnature07160"],"URL":"https:\/\/doi.org\/10.1038\/nature07160","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1118849.581231","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1118849.578855","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1118849.574945","asserted-by":"object"}]},"ISSN":["0028-0836","1476-4687"],"issn-type":[{"value":"0028-0836","type":"print"},{"value":"1476-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,7,23]]}}}