{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T00:35:06Z","timestamp":1792283706097,"version":"4.4.0"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"7521","license":[{"start":{"date-parts":[[2014,8,24]],"date-time":"2014-08-24T00:00:00Z","timestamp":1408838400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nature"],"published-print":{"date-parts":[[2014,10]]},"DOI":"10.1038\/nature13614","type":"journal-article","created":{"date-parts":[[2014,8,21]],"date-time":"2014-08-21T11:05:10Z","timestamp":1408619110000},"page":"242-246","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":162,"title":["HSP70 sequestration by free \u03b1-globin promotes ineffective erythropoiesis in \u03b2-thalassaemia"],"prefix":"10.1038","volume":"514","author":[{"given":"Jean-Beno\u00eet","family":"Arlet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Antoine","family":"Ribeil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia","family":"Guillem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Negre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adonis","family":"Hazoume","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Marcion","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Beuzard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u00ebl","family":"Dussiot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan Cruz","family":"Moura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Demarest","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isaure Chauvot","family":"de Beauch\u00eane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zakia","family":"Belaid-Choucair","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaux","family":"Sevin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago Trovati","family":"Maciel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Auclair","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Leboulch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stany","family":"Chretien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luba","family":"Tchertanov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Baudin-Creuza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renaud","family":"Seigneuric","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Fontenay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Garrido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Hermine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genevi\u00e8ve","family":"Courtois","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2014,8,24]]},"reference":[{"key":"BFnature13614_CR1","doi-asserted-by":"publisher","first-page":"1071","DOI":"10.1016\/j.hoc.2010.08.013","volume":"24","author":"E Khandros","year":"2010","unstructured":"Khandros, E. & Weiss, M. J. Protein quality control during erythropoiesis and hemoglobin synthesis. Hematol. Oncol. Clin. North Am. 24, 1071\u20131088 (2010)","journal-title":"Hematol. Oncol. Clin. North Am."},{"key":"BFnature13614_CR2","doi-asserted-by":"publisher","first-page":"4321","DOI":"10.1182\/blood-2011-03-283614","volume":"118","author":"Y Ginzburg","year":"2011","unstructured":"Ginzburg, Y. & Rivella, S. \u03b2-thalassemia: a model for elucidating the dynamic regulation of ineffective erythropoiesis and iron metabolism. Blood 118, 4321\u20134330 (2011)","journal-title":"Blood"},{"key":"BFnature13614_CR3","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1182\/blood.V82.2.374.374","volume":"82","author":"J Yuan","year":"1993","unstructured":"Yuan, J. et al. Accelerated programmed cell death (apoptosis) in erythroid precursors of patients with severe \u03b2-thalassemia (Cooley\u2019s anemia). Blood 82, 374\u2013377 (1993)","journal-title":"Blood"},{"key":"BFnature13614_CR4","doi-asserted-by":"publisher","first-page":"1343","DOI":"10.1016\/S0301-472X(00)00555-5","volume":"28","author":"LA Mathias","year":"2000","unstructured":"Mathias, L. A. et al. Ineffective erythropoiesis in \u03b2-thalassemia major is due to apoptosis at the polychromatophilic normoblast stage. Exp. Hematol. 28, 1343\u20131353 (2000)","journal-title":"Exp. Hematol."},{"key":"BFnature13614_CR5","doi-asserted-by":"publisher","first-page":"3624","DOI":"10.1182\/blood.V96.10.3624","volume":"96","author":"F Centis","year":"2000","unstructured":"Centis, F. et al. The importance of erythroid expansion in determining the extent of apoptosis in erythroid precursors in patients with \u03b2-thalassemia major. Blood 96, 3624\u20133629 (2000)","journal-title":"Blood"},{"key":"BFnature13614_CR6","doi-asserted-by":"publisher","first-page":"394295","DOI":"10.1155\/2013\/394295","volume":"2013","author":"JA Ribeil","year":"2013","unstructured":"Ribeil, J. A. et al. Ineffective erythropoiesis in \u03b2-thalassemia. ScientificWorldJournal 2013, 394295 (2013)","journal-title":"ScientificWorldJournal"},{"key":"BFnature13614_CR7","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1084\/jem.193.2.247","volume":"193","author":"Y Zermati","year":"2001","unstructured":"Zermati, Y. et al. Caspase activation is required for terminal erythroid differentiation. J. Exp. Med. 193, 247\u2013254 (2001)","journal-title":"J. Exp. Med."},{"key":"BFnature13614_CR8","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1038\/nature05378","volume":"445","author":"J-A Ribeil","year":"2007","unstructured":"Ribeil, J.-A. et al. Hsp70 regulates erythropoiesis by preventing caspase-3-mediated cleavage of GATA-1. Nature 445, 102\u2013105 (2007)","journal-title":"Nature"},{"key":"BFnature13614_CR9","doi-asserted-by":"publisher","first-page":"324","DOI":"10.1038\/nature10317","volume":"475","author":"FU Hartl","year":"2011","unstructured":"Hartl, F. U., Bracher, A. & Hayer-Hartl, M. Molecular chaperones in protein folding and proteostasis. Nature 475, 324\u2013332 (2011)","journal-title":"Nature"},{"key":"BFnature13614_CR10","doi-asserted-by":"publisher","first-page":"3246","DOI":"10.1182\/blood-2013-01-476390","volume":"121","author":"J Hu","year":"2013","unstructured":"Hu, J. et al. Isolation and functional characterization of human erythroblasts at distinct stages: implications for understanding of normal and disordered erythropoiesis in vivo. Blood 121, 3246\u20133253 (2013)","journal-title":"Blood"},{"key":"BFnature13614_CR11","doi-asserted-by":"publisher","first-page":"758","DOI":"10.1038\/nature00803","volume":"417","author":"AJ Kihm","year":"2002","unstructured":"Kihm, A. J. et al. An abundant erythroid protein that stabilizes free \u03b1-haemoglobin. Nature 417, 758\u2013763 (2002)","journal-title":"Nature"},{"key":"BFnature13614_CR12","doi-asserted-by":"publisher","first-page":"1532","DOI":"10.1182\/blood-2011-03-343475","volume":"119","author":"E Frisan","year":"2012","unstructured":"Frisan, E. et al. Defective nuclear localization of Hsp70 is associated with dyserythropoiesis and GATA-1 cleavage in myelodysplastic syndromes. Blood 119, 1532\u20131542 (2012)","journal-title":"Blood"},{"key":"BFnature13614_CR13","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1038\/46809","volume":"401","author":"R De Maria","year":"1999","unstructured":"De Maria, R. et al. Negative regulation of erythropoiesis by caspase-mediated cleavage of GATA-1. Nature 401, 489\u2013493 (1999)","journal-title":"Nature"},{"key":"BFnature13614_CR14","doi-asserted-by":"publisher","first-page":"1109","DOI":"10.1182\/blood.V69.4.1109.1109","volume":"69","author":"GJ Dover","year":"1987","unstructured":"Dover, G. J. & Boyer, S. H. Fetal hemoglobin-containing cells have the same mean corpuscular hemoglobin as cells without fetal hemoglobin: a reciprocal relationship between gamma- and beta-globin gene expression in normal subjects and in those with high fetal hemoglobin production. Blood 69, 1109\u20131113 (1987)","journal-title":"Blood"},{"key":"BFnature13614_CR15","doi-asserted-by":"publisher","first-page":"889","DOI":"10.1016\/j.exphem.2009.05.004","volume":"37","author":"X Yao","year":"2009","unstructured":"Yao, X. et al. Role of STAT3 and GATA-1 interactions in gamma-globin gene expression. Exp. Hematol. 37, 889\u2013900 (2009)","journal-title":"Exp. Hematol."},{"key":"BFnature13614_CR16","doi-asserted-by":"publisher","first-page":"6944","DOI":"10.1093\/nar\/gkr253","volume":"39","author":"Y Woon Kim","year":"2011","unstructured":"Woon Kim, Y., Kim, S., Geun Kim, C. & Kim, A. The distinctive roles of erythroid specific activator GATA-1 and NF-E2 in transcription of the human fetal \u03b3-globin genes. Nucleic Acids Res. 39, 6944\u20136955 (2011)","journal-title":"Nucleic Acids Res."},{"key":"BFnature13614_CR17","doi-asserted-by":"publisher","first-page":"3045","DOI":"10.1182\/blood-2010-07-294751","volume":"117","author":"J Zhu","year":"2011","unstructured":"Zhu, J. et al. Recombinant erythroid Kruppel-like factor fused to GATA1 up-regulates \u03b4- and \u03b3-globin expression in erythroid cells. Blood 117, 3045\u20133052 (2011)","journal-title":"Blood"},{"key":"BFnature13614_CR18","doi-asserted-by":"publisher","first-page":"a011643","DOI":"10.1101\/cshperspect.a011643","volume":"3","author":"VG Sankaran","year":"2013","unstructured":"Sankaran, V. G. & Orkin, S. H. The switch from fetal to adult hemoglobin. Cold Spring Harb. Perspect. Med. 3, a011643 (2013)","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"BFnature13614_CR19","doi-asserted-by":"publisher","first-page":"875","DOI":"10.1182\/blood-2007-12-126938","volume":"112","author":"IV Libani","year":"2008","unstructured":"Libani, I. V. et al. Decreased differentiation of erythroid cells exacerbates ineffective erythropoiesis in beta-thalassemia. Blood 112, 875\u2013885 (2008)","journal-title":"Blood"},{"key":"BFnature13614_CR20","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1038\/nm.3126","volume":"19","author":"P Ramos","year":"2013","unstructured":"Ramos, P. et al. Macrophages support pathological erythropoiesis in polycythemia vera and \u03b2-thalassemia. Nature Med. 19, 437\u2013445 (2013)","journal-title":"Nature Med."},{"key":"BFnature13614_CR21","doi-asserted-by":"publisher","first-page":"398","DOI":"10.1038\/nm.3468","volume":"20","author":"M Dussiot","year":"2014","unstructured":"Dussiot, M. et al. An activin receptor IIA ligand trap corrects ineffective erythropoiesis in \u03b2-thalassemia. Nature Med. 20, 398\u2013407 (2014)","journal-title":"Nature Med."},{"key":"BFnature13614_CR22","doi-asserted-by":"publisher","unstructured":"Suragani, R. N. V. S. et al. Modified activin receptor IIB ligand trap mitigates ineffective erythropoiesis and disease complications in murine \u03b2-thalassemia. Blood http:\/\/dx.doi.org\/10.1182\/blood-2013-06-511238 (19 June 2014)","DOI":"10.1182\/blood-2013-06-511238"},{"key":"BFnature13614_CR23","doi-asserted-by":"publisher","first-page":"1319","DOI":"10.3324\/haematol.11556","volume":"92","author":"L De Franceschi","year":"2007","unstructured":"De Franceschi, L. et al. K-CL co-transport plays an important role in normal and beta thalassemic erythropoiesis. Haematologica 92, 1319\u20131326 (2007)","journal-title":"Haematologica"},{"key":"BFnature13614_CR24","doi-asserted-by":"publisher","first-page":"885","DOI":"10.1016\/S0301-472X(00)00488-4","volume":"28","author":"Y Zermati","year":"2000","unstructured":"Zermati, Y. et al. Transforming growth factor inhibits erythropoiesis by blocking proliferation and accelerating differentiation of erythroid progenitors. Exp. Hematol. 28, 885\u2013894 (2000)","journal-title":"Exp. Hematol."},{"key":"BFnature13614_CR25","doi-asserted-by":"publisher","first-page":"678","DOI":"10.1038\/cdd.2010.140","volume":"18","author":"A-S Gabet","year":"2011","unstructured":"Gabet, A.-S. et al. Caspase-activated ROCK-1 allows erythroblast terminal maturation independently of cytokine-induced Rho signaling. Cell Death Differ. 18, 678\u2013689 (2011)","journal-title":"Cell Death Differ."},{"key":"BFnature13614_CR26","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1111\/j.1365-2818.2006.01706.x","volume":"224","author":"S Bolte","year":"2006","unstructured":"Bolte, S. & Cordeli\u00e8res, F. P. A guided tour into subcellular colocalization analysis in light microscopy. J. Microsc. 224, 213\u2013232 (2006)","journal-title":"J. Microsc."},{"key":"BFnature13614_CR27","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1038\/nmeth974","volume":"4","author":"E Schallmeiner","year":"2007","unstructured":"Schallmeiner, E. et al. Sensitive protein detection via triple-binder proximity ligation assays. Nature Methods 4, 135\u2013137 (2007)","journal-title":"Nature Methods"},{"key":"BFnature13614_CR28","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/j.bbagen.2013.09.015","volume":"1840","author":"L Kiger","year":"2014","unstructured":"Kiger, L. et al. Dynamics of \u03b1-Hb chain binding to its chaperone AHSP depends on heme coordination and redox state. Biochim. Biophys. Acta 1840, 277\u2013287 (2014)","journal-title":"Biochim. Biophys. Acta"},{"key":"BFnature13614_CR29","doi-asserted-by":"publisher","first-page":"27","DOI":"10.3109\/03630267609031020","volume":"1","author":"EC Abraham","year":"1976","unstructured":"Abraham, E. C., Reese, A., Stallings, M. & Huisman, T. H. Separation of human hemoglobins by DEAE-cellulose chromatography using glycine-KCN-NaC1 developers. Hemoglobin 1, 27\u201344 (1976)","journal-title":"Hemoglobin"},{"key":"BFnature13614_CR30","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1016\/S0021-9258(18)97685-4","volume":"240","author":"E Bucci","year":"1965","unstructured":"Bucci, E. & Fronticelli, C. A new method for the preparation of alpha and beta subunits of human hemoglobin. J. Biol. Chem. 240, 551\u2013552 (1965)","journal-title":"J. Biol. Chem."},{"key":"BFnature13614_CR31","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1085\/jgp.13.4.469","volume":"13","author":"ML Anson","year":"1930","unstructured":"Anson, M. L. & Mirsky, A. E. Protein coagulation andits reversal: the preparation of insoluble globin, soluble globin and heme. J. Gen. Physiol. 13, 469\u2013476 (1930)","journal-title":"J. Gen. Physiol."}],"container-title":["Nature"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nature13614.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nature13614","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nature13614.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T14:23:43Z","timestamp":1684419823000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nature13614"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,24]]},"references-count":31,"journal-issue":{"issue":"7521","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["BFnature13614"],"URL":"https:\/\/doi.org\/10.1038\/nature13614","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.718544629.793540282","asserted-by":"object"}]},"ISSN":["0028-0836","1476-4687"],"issn-type":[{"value":"0028-0836","type":"print"},{"value":"1476-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,24]]},"assertion":[{"value":"23 February 2013","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 June 2014","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 August 2014","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}