{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:49:45Z","timestamp":1792615785708,"version":"4.5.0"},"reference-count":48,"publisher":"Springer Science and Business Media LLC","issue":"7629","license":[{"start":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T00:00:00Z","timestamp":1478476800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T00:00:00Z","timestamp":1478476800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nature"],"published-print":{"date-parts":[[2016,11,17]]},"DOI":"10.1038\/nature20134","type":"journal-article","created":{"date-parts":[[2016,11,4]],"date-time":"2016-11-04T13:03:11Z","timestamp":1478264591000},"page":"384-389","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":868,"title":["CRISPR\/Cas9 \u03b2-globin gene targeting in human haematopoietic stem cells"],"prefix":"10.1038","volume":"539","author":[{"given":"Daniel P.","family":"Dever","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasmus O.","family":"Bak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Reinisch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joab","family":"Camarena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Washington","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmencita E.","family":"Nicolas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mara","family":"Pavel-Dinu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nivi","family":"Saxena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alec B.","family":"Wilkens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sruthi","family":"Mantri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuko","family":"Uchida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayal","family":"Hendel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anupama","family":"Narla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravindra","family":"Majeti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth I.","family":"Weinberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew H.","family":"Porteus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,11,7]]},"reference":[{"key":"BFnature20134_CR1","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1038\/nrg2985","volume":"12","author":"L Naldini","year":"2011","unstructured":"Naldini, L. Ex vivo gene transfer and correction for cell-based therapies. Nat. Rev. Genet. 12, 301\u2013315 (2011)","journal-title":"Nat. Rev. Genet."},{"key":"BFnature20134_CR2","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1146\/annurev-pharmtox-010814-124454","volume":"56","author":"M Porteus","year":"2016","unstructured":"Porteus, M. Genome editing: a new approach to human therapeutics. Annu. Rev. Pharmacol. Toxicol. 56, 163\u2013190 (2016)","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"BFnature20134_CR3","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1038\/317230a0","volume":"317","author":"O Smithies","year":"1985","unstructured":"Smithies, O., Gregg, R. G., Boggs, S. S., Koralewski, M. A. & Kucherlapati, R. S. Insertion of DNA sequences into the human chromosomal \u03b2-globin locus by homologous recombination. Nature 317, 230\u2013234 (1985)","journal-title":"Nature"},{"key":"BFnature20134_CR4","doi-asserted-by":"publisher","first-page":"763","DOI":"10.1126\/science.1078395","volume":"300","author":"MH Porteus","year":"2003","unstructured":"Porteus, M. H. & Baltimore, D. Chimeric nucleases stimulate gene targeting in human cells. Science 300, 763 (2003)","journal-title":"Science"},{"key":"BFnature20134_CR5","doi-asserted-by":"publisher","first-page":"6064","DOI":"10.1073\/pnas.91.13.6064","volume":"91","author":"P Rouet","year":"1994","unstructured":"Rouet, P., Smih, F. & Jasin, M. Expression of a site-specific endonuclease stimulates homologous recombination in mammalian cells. Proc. Natl Acad. Sci. USA 91, 6064\u20136068 (1994)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature20134_CR6","doi-asserted-by":"publisher","first-page":"1262","DOI":"10.1016\/j.cell.2014.05.010","volume":"157","author":"PD Hsu","year":"2014","unstructured":"Hsu, P. D., Lander, E. S. & Zhang, F. Development and applications of CRISPR-Cas9 for genome engineering. Cell 157, 1262\u20131278 (2014)","journal-title":"Cell"},{"key":"BFnature20134_CR7","doi-asserted-by":"publisher","first-page":"1258096","DOI":"10.1126\/science.1258096","volume":"346","author":"JA Doudna","year":"2014","unstructured":"Doudna, J. A. & Charpentier, E. Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science 346, 1258096 (2014)","journal-title":"Science"},{"key":"BFnature20134_CR8","doi-asserted-by":"publisher","first-page":"816","DOI":"10.1126\/science.1225829","volume":"337","author":"M Jinek","year":"2012","unstructured":"Jinek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816\u2013821 (2012)","journal-title":"Science"},{"key":"BFnature20134_CR9","doi-asserted-by":"publisher","first-page":"3703","DOI":"10.1016\/j.febslet.2010.07.057","volume":"584","author":"EM Kass","year":"2010","unstructured":"Kass, E. M. & Jasin, M. Collaboration and competition between DNA double-strand break repair pathways. FEBS Lett . 584, 3703\u20133708 (2010)","journal-title":"FEBS Lett"},{"key":"BFnature20134_CR10","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1186\/s13059-015-0859-y","volume":"16","author":"MH Porteus","year":"2015","unstructured":"Porteus, M. H. Towards a new era in medicine: therapeutic genome editing. Genome Biol . 16, 286 (2015)","journal-title":"Genome Biol"},{"key":"BFnature20134_CR11","doi-asserted-by":"publisher","first-page":"1123","DOI":"10.1038\/4401123a","volume":"440","author":"NB Woods","year":"2006","unstructured":"Woods, N. B., Bottero, V., Schmidt, M., von Kalle, C. & Verma, I. M. Gene therapy: therapeutic gene causing lymphoma. Nature 440, 1123 (2006)","journal-title":"Nature"},{"key":"BFnature20134_CR12","doi-asserted-by":"publisher","first-page":"2513","DOI":"10.1182\/blood-2015-11-683235","volume":"127","author":"N Hubbard","year":"2016","unstructured":"Hubbard, N. et al. Targeted gene editing restores regulated CD40L expression and function in X-HIGM T cells. Blood 127, 2513\u20132522 (2016)","journal-title":"Blood"},{"key":"BFnature20134_CR13","doi-asserted-by":"publisher","first-page":"1365","DOI":"10.1093\/nar\/gkt947","volume":"42","author":"RA Voit","year":"2014","unstructured":"Voit, R. A., Hendel, A., Pruett-Miller, S. M. & Porteus, M. H. Nuclease-mediated gene editing by homologous recombination of the human globin locus. Nucleic Acids Res . 42, 1365\u20131378 (2014)","journal-title":"Nucleic Acids Res"},{"key":"BFnature20134_CR14","doi-asserted-by":"publisher","first-page":"985","DOI":"10.1038\/nbt.3290","volume":"33","author":"A Hendel","year":"2015","unstructured":"Hendel, A. et al. Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Nat. Biotechnol. 33, 985\u2013989 (2015)","journal-title":"Nat. Biotechnol."},{"key":"BFnature20134_CR15","doi-asserted-by":"publisher","first-page":"2804","DOI":"10.1073\/pnas.89.7.2804","volume":"89","author":"CM Baum","year":"1992","unstructured":"Baum, C. M., Weissman, I. L., Tsukamoto, A. S., Buckle, A. M. & Peault, B. Isolation of a candidate human hematopoietic stem-cell population. Proc. Natl Acad. Sci. USA 89, 2804\u20132808 (1992)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature20134_CR16","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1016\/j.gene.2013.03.098","volume":"525","author":"S Mukherjee","year":"2013","unstructured":"Mukherjee, S. & Thrasher, A. J. Gene therapy for PIDs: progress, pitfalls and prospects. Gene 525, 174\u2013181 (2013)","journal-title":"Gene"},{"key":"BFnature20134_CR17","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1038\/nature09328","volume":"467","author":"M Cavazzana-Calvo","year":"2010","unstructured":"Cavazzana-Calvo, M. et al. Transfusion independence and HMGA2 activation after gene therapy of human \u03b2-thalassaemia. Nature 467, 318\u2013322 (2010)","journal-title":"Nature"},{"key":"BFnature20134_CR18","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1038\/nature15818","volume":"526","author":"L Naldini","year":"2015","unstructured":"Naldini, L. Gene therapy returns to centre stage. Nature 526, 351\u2013360 (2015)","journal-title":"Nature"},{"key":"BFnature20134_CR19","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1038\/nrc2804","volume":"10","author":"RR Jenq","year":"2010","unstructured":"Jenq, R. R. & van den Brink, M. R. Allogeneic haematopoietic stem cell transplantation: individualized stem cell and immune therapy of cancer. Nat. Rev. Cancer 10, 213\u2013221 (2010)","journal-title":"Nat. Rev. Cancer"},{"key":"BFnature20134_CR20","doi-asserted-by":"publisher","first-page":"2597","DOI":"10.1182\/blood-2014-12-615948","volume":"125","author":"MD Hoban","year":"2015","unstructured":"Hoban, M. D. et al. Correction of the sickle cell disease mutation in human hematopoietic stem\/progenitor cells. Blood 125, 2597\u20132604 (2015)","journal-title":"Blood"},{"key":"BFnature20134_CR21","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1038\/nature13420","volume":"510","author":"P Genovese","year":"2014","unstructured":"Genovese, P. et al. Targeted genome editing in human repopulating haematopoietic stem cells. Nature 510, 235\u2013240 (2014)","journal-title":"Nature"},{"key":"BFnature20134_CR22","doi-asserted-by":"publisher","first-page":"1256","DOI":"10.1038\/nbt.3408","volume":"33","author":"J Wang","year":"2015","unstructured":"Wang, J. et al. Homology-driven genome editing in hematopoietic stem and progenitor cells using ZFN mRNA and AAV6 donors. Nat. Biotechnol. 33, 1256\u20131263 (2015)","journal-title":"Nat. Biotechnol."},{"key":"BFnature20134_CR23","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1038\/nbt.3513","volume":"34","author":"SS De Ravin","year":"2016","unstructured":"De Ravin, S. S. et al. Targeted gene addition in human CD34+ hematopoietic cells for correction of X-linked chronic granulomatous disease. Nat. Biotechnol. 34, 424\u2013429 (2016)","journal-title":"Nat. Biotechnol."},{"key":"BFnature20134_CR24","first-page":"307ra156","volume":"7","author":"BD Sather","year":"2015","unstructured":"Sather, B. D. et al. Efficient modification of CCR5 in primary human hematopoietic cells using a megaTAL nuclease and AAV donor template. Sci. Transl. Med. 7, 307ra156 (2015)","journal-title":"Sci. Transl. Med."},{"key":"BFnature20134_CR25","doi-asserted-by":"publisher","first-page":"e30","DOI":"10.1093\/nar\/gkv1121","volume":"44","author":"J Wang","year":"2016","unstructured":"Wang, J. et al. Highly efficient homology-driven genome editing in human T cells by combining zinc-finger nuclease mRNA and AAV6 donor delivery. Nucleic Acids Res . 44, e30 (2016)","journal-title":"Nucleic Acids Res"},{"key":"BFnature20134_CR26","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1038\/ng1380","volume":"36","author":"DG Miller","year":"2004","unstructured":"Miller, D. G., Petek, L. M. & Russell, D. W. Adeno-associated virus vectors integrate at chromosome breakage sites. Nat. Genet. 36, 767\u2013773 (2004)","journal-title":"Nat. Genet."},{"key":"BFnature20134_CR27","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1038\/ng0498-325","volume":"18","author":"DW Russell","year":"1998","unstructured":"Russell, D. W. & Hirata, R. K. Human gene targeting by viral vectors. Nat. Genet. 18, 325\u2013330 (1998)","journal-title":"Nat. Genet."},{"key":"BFnature20134_CR28","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1038\/nature13864","volume":"517","author":"A Barzel","year":"2015","unstructured":"Barzel, A. et al. Promoterless gene targeting without nucleases ameliorates haemophilia B in mice. Nature 517, 360\u2013364 (2015)","journal-title":"Nature"},{"key":"BFnature20134_CR29","doi-asserted-by":"publisher","first-page":"839","DOI":"10.1182\/blood-2015-09-618587","volume":"127","author":"MD Hoban","year":"2016","unstructured":"Hoban, M. D., Orkin, S. H. & Bauer, D. E. Genetic treatment of a molecular disorder: gene therapy approaches to sickle cell disease. Blood 127, 839\u2013848 (2016)","journal-title":"Blood"},{"key":"BFnature20134_CR30","doi-asserted-by":"publisher","first-page":"1719","DOI":"10.1126\/science.276.5319.1719","volume":"276","author":"C Bonini","year":"1997","unstructured":"Bonini, C. et al. HSV-TK gene transfer into donor lymphocytes for control of allogeneic graft-versus-leukemia. Science 276, 1719\u20131724 (1997)","journal-title":"Science"},{"key":"BFnature20134_CR31","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1016\/S1470-2045(09)70074-9","volume":"10","author":"F Ciceri","year":"2009","unstructured":"Ciceri, F. et al. Infusion of suicide-gene-engineered donor lymphocytes after family haploidentical haemopoietic stem-cell transplantation for leukaemia (the TK007 trial): a non-randomised phase I-II study. Lancet Oncol . 10, 489\u2013500 (2009)","journal-title":"Lancet Oncol"},{"key":"BFnature20134_CR32","first-page":"317ra198","volume":"7","author":"G Oliveira","year":"2015","unstructured":"Oliveira, G. et al. Tracking genetically engineered lymphocytes long-term reveals the dynamics of T cell immunological memory. Sci. Transl. Med. 7, 317ra198 (2015)","journal-title":"Sci. Transl. Med."},{"key":"BFnature20134_CR33","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1038\/nm0403-367","volume":"9","author":"C Bonini","year":"2003","unstructured":"Bonini, C. et al. Safety of retroviral gene marking with a truncated NGF receptor. Nat. Med. 9, 367\u2013369 (2003)","journal-title":"Nat. Med."},{"key":"BFnature20134_CR34","first-page":"640","volume":"2","author":"J Seita","year":"2010","unstructured":"Seita, J. & Weissman, I. L. Hematopoietic stem cell: self-renewal versus differentiation. Wiley Interdiscip. Rev. Syst. Biol. Med. 2, 640\u2013653 (2010)","journal-title":"Rev. Syst. Biol. Med."},{"key":"BFnature20134_CR35","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1016\/j.stem.2007.10.001","volume":"1","author":"R Majeti","year":"2007","unstructured":"Majeti, R., Park, C. Y. & Weissman, I. L. Identification of a hierarchy of multipotent hematopoietic progenitors in human cord blood. Cell Stem Cell 1, 635\u2013645 (2007)","journal-title":"Cell Stem Cell"},{"key":"BFnature20134_CR36","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/j.stem.2012.01.006","volume":"10","author":"S Doulatov","year":"2012","unstructured":"Doulatov, S., Notta, F., Laurenti, E. & Dick, J. E. Hematopoiesis: a human perspective. Cell Stem Cell 10, 120\u2013136 (2012)","journal-title":"Cell Stem Cell"},{"key":"BFnature20134_CR37","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1089\/hgtb.2014.043","volume":"25","author":"A Gu","year":"2014","unstructured":"Gu, A. et al. Engraftment and lineage potential of adult hematopoietic stem and progenitor cells is compromised following short-term culture in the presence of an aryl hydrocarbon receptor antagonist. Hum. Gene Ther. Methods 25, 221\u2013231 (2014)","journal-title":"Hum. Gene Ther. Methods"},{"key":"BFnature20134_CR38","doi-asserted-by":"publisher","first-page":"27518","DOI":"10.1074\/jbc.M402578200","volume":"279","author":"DN Levasseur","year":"2004","unstructured":"Levasseur, D. N. et al. A recombinant human hemoglobin with anti-sickling properties greater than fetal hemoglobin. J. Biol. Chem. 279, 27518\u201327524 (2004)","journal-title":"J. Biol. Chem."},{"key":"BFnature20134_CR39","doi-asserted-by":"publisher","first-page":"1481","DOI":"10.1182\/blood-2015-09-667923","volume":"127","author":"BM Dulmovits","year":"2016","unstructured":"Dulmovits, B. M. et al. Pomalidomide reverses \u03b3-globin silencing through the transcriptional reprogramming of adult hematopoietic progenitors. Blood 127, 1481\u20131492 (2016)","journal-title":"Blood"},{"key":"BFnature20134_CR40","doi-asserted-by":"publisher","first-page":"3246","DOI":"10.1182\/blood-2013-01-476390","volume":"121","author":"J Hu","year":"2013","unstructured":"Hu, J. et al. Isolation and functional characterization of human erythroblasts at distinct stages: implications for understanding of normal and disordered erythropoiesis in vivo. Blood 121, 3246\u20133253 (2013)","journal-title":"Blood"},{"key":"BFnature20134_CR41","doi-asserted-by":"publisher","first-page":"3317","DOI":"10.1172\/JCI67930","volume":"123","author":"Z Romero","year":"2013","unstructured":"Romero, Z. et al.. \u03b2-globin gene transfer to human bone marrow for sickle cell disease. J. Clin. Invest. 123, 3317\u20133330 (2013)","journal-title":"J. Clin. Invest."},{"key":"BFnature20134_CR42","doi-asserted-by":"publisher","first-page":"1509","DOI":"10.1126\/science.1256337","volume":"345","author":"I Fares","year":"2014","unstructured":"Fares, I. et al. Cord blood expansion. Pyrimidoindole derivatives are agonists of human hematopoietic stem cell self-renewal. Science 345, 1509\u20131512 (2014)","journal-title":"Science"},{"key":"BFnature20134_CR43","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1038\/nprot.2011.301","volume":"6","author":"IF Khan","year":"2011","unstructured":"Khan, I. F., Hirata, R. K. & Russell, D. W. AAV-mediated gene targeting methods for human cells. Nat. Protocols 6, 482\u2013501 (2011)","journal-title":"Nat. Protocols"},{"key":"BFnature20134_CR44","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1089\/hgtb.2011.034","volume":"23","author":"C Aurnhammer","year":"2012","unstructured":"Aurnhammer, C. et al. Universal real-time PCR for the detection and quantification of adeno-associated virus serotype 2-derived inverted terminal repeat sequences. Hum. Gene Ther. Methods 23, 18\u201328 (2012)","journal-title":"Hum. Gene Ther. Methods"},{"key":"BFnature20134_CR45","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1016\/j.celrep.2014.02.040","volume":"7","author":"A Hendel","year":"2014","unstructured":"Hendel, A. et al. Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing. Cell Reports 7, 293\u2013305 (2014)","journal-title":"Cell Reports"},{"key":"BFnature20134_CR46","doi-asserted-by":"publisher","first-page":"9584","DOI":"10.1093\/nar\/gkt714","volume":"41","author":"TJ Cradick","year":"2013","unstructured":"Cradick, T. J., Fine, E. J., Antico, C. J. & Bao, G. CRISPR\/Cas9 systems targeting \u03b2-globin and CCR5 genes have substantial off-target activity. Nucleic Acids Res . 41, 9584\u20139592 (2013)","journal-title":"Nucleic Acids Res"},{"key":"BFnature20134_CR47","doi-asserted-by":"publisher","first-page":"e168","DOI":"10.1093\/nar\/gku936","volume":"42","author":"EK Brinkman","year":"2014","unstructured":"Brinkman, E. K., Chen, T., Amendola, M. & van Steensel, B. Easy quantitative assessment of genome editing by sequence trace decomposition. Nucleic Acids Res . 42, e168 (2014)","journal-title":"Nucleic Acids Res"},{"key":"BFnature20134_CR48","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1002\/ajh.2830160309","volume":"16","author":"DR Boggs","year":"1984","unstructured":"Boggs, D. R. The total marrow mass of the mouse: a simplified method of measurement. Am. J. Hematol. 16, 277\u2013286 (1984)","journal-title":"Am. J. Hematol."}],"container-title":["Nature"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/nature20134.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/nature20134","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/nature20134.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,15]],"date-time":"2022-04-15T10:10:32Z","timestamp":1650017432000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nature20134"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,7]]},"references-count":48,"journal-issue":{"issue":"7629","published-print":{"date-parts":[[2016,11,17]]}},"alternative-id":["BFnature20134"],"URL":"https:\/\/doi.org\/10.1038\/nature20134","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.726936889.793549664","asserted-by":"object"}]},"ISSN":["0028-0836","1476-4687"],"issn-type":[{"value":"0028-0836","type":"print"},{"value":"1476-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,7]]},"assertion":[{"value":"20 May 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 September 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 November 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"M.H.P. is a consultant and has equity interest in CRISPR Tx, but CRISPR Tx had no input into the design, execution, interpretation or publication of the results herein. N.U. was a former employee of Stem Cells, Inc., but they had no input into this manuscript.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}