{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T23:09:35Z","timestamp":1792105775883,"version":"4.4.0"},"reference-count":56,"publisher":"Springer Science and Business Media LLC","issue":"7652","license":[{"start":{"date-parts":[[2017,4,26]],"date-time":"2017-04-26T00:00:00Z","timestamp":1493164800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2017,4,26]],"date-time":"2017-04-26T00:00:00Z","timestamp":1493164800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nature"],"published-print":{"date-parts":[[2017,5,4]]},"DOI":"10.1038\/nature22330","type":"journal-article","created":{"date-parts":[[2017,4,25]],"date-time":"2017-04-25T11:05:49Z","timestamp":1493118349000},"page":"54-59","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1374,"title":["Assembly of functionally integrated human forebrain spheroids"],"prefix":"10.1038","volume":"545","author":[{"given":"Fikri","family":"Birey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jimena","family":"Andersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher D.","family":"Makinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saiful","family":"Islam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Huber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. Christina","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly R. Cordes","family":"Metzler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgia","family":"Panagiotakos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Thom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy A.","family":"O\u2019Rourke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars M.","family":"Steinmetz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan A.","family":"Bernstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Hallmayer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Huguenard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergiu P.","family":"Pa\u015fca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,4,26]]},"reference":[{"key":"BFnature22330_CR1","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1038\/nature12983","volume":"505","author":"A Kepecs","year":"2014","unstructured":"Kepecs, A. & Fishell, G. Interneuron cell types are fit to function. Nature 505, 318\u2013326 (2014)","journal-title":"Nature"},{"key":"BFnature22330_CR2","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1038\/nrn1954","volume":"7","author":"CP Wonders","year":"2006","unstructured":"Wonders, C. P. & Anderson, S. A. The origin and specification of cortical interneurons. Nat. Rev. Neurosci. 7, 687\u2013696 (2006)","journal-title":"Nat. Rev. Neurosci."},{"key":"BFnature22330_CR3","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/j.neuron.2015.12.008","volume":"89","author":"JC Silbereis","year":"2016","unstructured":"Silbereis, J. C., Pochareddy, S., Zhu, Y., Li, M. & Sestan, N. The cellular and molecular landscapes of the developing human central nervous system. Neuron 89, 248\u2013268 (2016)","journal-title":"Neuron"},{"key":"BFnature22330_CR4","doi-asserted-by":"publisher","first-page":"849","DOI":"10.1016\/j.neuron.2013.08.014","volume":"79","author":"G Bartolini","year":"2013","unstructured":"Bartolini, G., Ciceri, G. & Mar\u00edn, O. Integration of GABAergic interneurons into cortical cell assemblies: lessons from embryos and adults. Neuron 79, 849\u2013864 (2013)","journal-title":"Neuron"},{"key":"BFnature22330_CR5","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1034\/j.1601-183X.2003.00037.x","volume":"2","author":"JL Rubenstein","year":"2003","unstructured":"Rubenstein, J. L. & Merzenich, M. M. Model of autism: increased ratio of excitation\/inhibition in key neural systems. Genes Brain Behav. 2, 255\u2013267 (2003)","journal-title":"Genes Brain Behav"},{"key":"BFnature22330_CR6","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1038\/nrn3155","volume":"13","author":"O Mar\u00edn","year":"2012","unstructured":"Mar\u00edn, O. Interneuron dysfunction in psychiatric disorders. Nat. Rev. Neurosci. 13, 107\u2013120 (2012)","journal-title":"Nat. Rev. Neurosci."},{"key":"BFnature22330_CR7","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1038\/nrg3563","volume":"15","author":"V Tabar","year":"2014","unstructured":"Tabar, V. & Studer, L. Pluripotent stem cells in regenerative medicine: challenges and recent progress. Nat. Rev. Genet. 15, 82\u201392 (2014)","journal-title":"Nat. Rev. Genet."},{"key":"BFnature22330_CR8","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1146\/annurev-neuro-062012-170328","volume":"37","author":"SP Pas\u00b8ca","year":"2014","unstructured":"Pas\u00b8ca, S. P., Panagiotakos, G. & Dolmetsch, R. E. Generating human neurons in vitro and using them to understand neuropsychiatric disease. Annu. Rev. Neurosci. 37, 479\u2013501 (2014)","journal-title":"Annu. Rev. Neurosci."},{"key":"BFnature22330_CR9","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1016\/j.stem.2013.04.008","volume":"12","author":"AM Maroof","year":"2013","unstructured":"Maroof, A. M. et al. Directed differentiation and functional maturation of cortical interneurons from human embryonic stem cells. Cell Stem Cell 12, 559\u2013572 (2013)","journal-title":"Cell Stem Cell"},{"key":"BFnature22330_CR10","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1016\/j.stem.2013.04.005","volume":"12","author":"CR Nicholas","year":"2013","unstructured":"Nicholas, C. R. et al. Functional maturation of hPSC-derived forebrain interneurons requires an extended timeline and mimics human neural development. Cell Stem Cell 12, 573\u2013586 (2013)","journal-title":"Cell Stem Cell"},{"key":"BFnature22330_CR11","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1038\/nmeth.3415","volume":"12","author":"AM Pas\u00b8ca","year":"2015","unstructured":"Pas\u00b8ca, A. M. et al. Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture. Nat. Methods 12, 671\u2013678 (2015)","journal-title":"Nat. Methods"},{"key":"BFnature22330_CR12","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1159\/000066268","volume":"1","author":"HBM Uylings","year":"2002","unstructured":"Uylings, H. B. M., Delalle, I., Petanjek, Z. & Koenderink, M. J. T. Structural and immunocytochemical differentiation of neurons in prenatal and postnatal human prefrontal cortex. Neuroembryology 1, 176\u2013186 (2002)","journal-title":"Neuroembryology"},{"key":"BFnature22330_CR13","doi-asserted-by":"publisher","first-page":"1258367","DOI":"10.1126\/science.1258367","volume":"347","author":"HC Fan","year":"2015","unstructured":"Fan, H. C., Fu, G. K. & Fodor, S. P. Expression profiling. Combinatorial labeling of single cells for gene expression cytometry. Science 347, 1258367 (2015)","journal-title":"Science"},{"key":"BFnature22330_CR14","first-page":"2579","volume":"9","author":"L van der Maaten","year":"2008","unstructured":"van der Maaten, L. & Hinton, G. Visualizing data using t-SNE. J. Mach. Learn. Res. 9, 2579\u20132605 (2008)","journal-title":"J. Mach. Learn. Res."},{"key":"BFnature22330_CR15","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1016\/j.mcn.2008.10.003","volume":"40","author":"GB Potter","year":"2009","unstructured":"Potter, G. B. et al. Generation of Cre-transgenic mice using Dlx1\/Dlx2 enhancers and their characterization in GABAergic interneurons. Mol. Cell. Neurosci. 40, 167\u2013186 (2009)","journal-title":"Mol. Cell. Neurosci."},{"key":"BFnature22330_CR16","doi-asserted-by":"publisher","first-page":"e13073","DOI":"10.7554\/eLife.13073","volume":"5","author":"Y Sun","year":"2016","unstructured":"Sun, Y. et al. A deleterious Nav1.1 mutation selectively impairs telencephalic inhibitory neurons derived from Dravet syndrome patients. eLife 5, e13073 (2016)","journal-title":"eLife"},{"key":"BFnature22330_CR17","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.neuron.2009.01.034","volume":"62","author":"D Bortone","year":"2009","unstructured":"Bortone, D. & Polleux, F. KCC2 expression promotes the termination of cortical interneuron migration in a voltage-sensitive calcium-dependent manner. Neuron 62, 53\u201371 (2009)","journal-title":"Neuron"},{"key":"BFnature22330_CR18","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1038\/nn813","volume":"5","author":"B Nadarajah","year":"2002","unstructured":"Nadarajah, B., Alifragis, P., Wong, R. O. & Parnavelas, J. G. Ventricle-directed migration in the developing cerebral cortex. Nat. Neurosci. 5, 218\u2013224 (2002)","journal-title":"Nat. Neurosci."},{"key":"BFnature22330_CR19","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1242\/dev.128.3.353","volume":"128","author":"SA Anderson","year":"2001","unstructured":"Anderson, S. A., Mar\u00edn, O., Horn, C., Jennings, K. & Rubenstein, J. L. Distinct cortical migrations from the medial and lateral ganglionic eminences. Development 128, 353\u2013363 (2001)","journal-title":"Development"},{"key":"BFnature22330_CR20","doi-asserted-by":"publisher","first-page":"5691","DOI":"10.1523\/JNEUROSCI.1030-05.2005","volume":"25","author":"A Bellion","year":"2005","unstructured":"Bellion, A., Baudoin, J. P., Alvarez, C., Bornens, M. & M\u00e9tin, C. Nucleokinesis in tangentially migrating neurons comprises two alternating phases: forward migration of the Golgi\/centrosome associated with centrosome splitting and myosin contraction at the rear. J. Neurosci. 25, 5691\u20135699 (2005)","journal-title":"J. Neurosci."},{"key":"BFnature22330_CR21","doi-asserted-by":"publisher","first-page":"5123","DOI":"10.1523\/JNEUROSCI.23-12-05123.2003","volume":"23","author":"RK Stumm","year":"2003","unstructured":"Stumm, R. K. et al. CXCR4 regulates interneuron migration in the developing neocortex. J. Neurosci. 23, 5123\u20135130 (2003)","journal-title":"J. Neurosci."},{"key":"BFnature22330_CR22","doi-asserted-by":"publisher","first-page":"1371","DOI":"10.1016\/S0140-6736(12)62129-1","volume":"381","author":"Group Cross-Disorder","year":"2013","unstructured":"Cross-Disorder Group of the Psychiatric Genomics Consortium. Identification of risk loci with shared effects on five major psychiatric disorders: a genome-wide analysis. Lancet 381, 1371\u20131379 (2013)","journal-title":"Lancet"},{"key":"BFnature22330_CR23","doi-asserted-by":"crossref","unstructured":"Schizophrenia Working Group of the Psychiatric Genomics Consortium. Biological insights from 108 schizophrenia-associated genetic loci. Nature 511, 421\u2013427 (2014)","DOI":"10.1038\/nature13595"},{"key":"BFnature22330_CR24","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.cell.2004.09.011","volume":"119","author":"I Splawski","year":"2004","unstructured":"Splawski, I. et al. CaV1.2 calcium channel dysfunction causes a multisystem disorder including arrhythmia and autism. Cell 119, 19\u201331 (2004)","journal-title":"Cell"},{"key":"BFnature22330_CR25","doi-asserted-by":"publisher","first-page":"8089","DOI":"10.1073\/pnas.0502506102","volume":"102","author":"I Splawski","year":"2005","unstructured":"Splawski, I. et al. Severe arrhythmia disorder caused by cardiac L-type calcium channel mutations. Proc. Natl Acad. Sci. USA 102, 8089\u20138096 (2005)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature22330_CR26","doi-asserted-by":"publisher","first-page":"1657","DOI":"10.1038\/nm.2576","volume":"17","author":"SP Pas\u00b8ca","year":"2011","unstructured":"Pas\u00b8ca, S. P. et al. Using iPSC-derived neurons to uncover cellular phenotypes associated with Timothy syndrome. Nat. Med. 17, 1657\u20131662 (2011)","journal-title":"Nat. Med."},{"key":"BFnature22330_CR27","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1074\/jbc.M109.076448","volume":"285","author":"V Yarotskyy","year":"2010","unstructured":"Yarotskyy, V. et al. Roscovitine binds to novel L-channel (CaV1.2) sites that separately affect activation and inactivation. J. Biol. Chem. 285, 43\u201353 (2010)","journal-title":"J. Biol. Chem."},{"key":"BFnature22330_CR28","doi-asserted-by":"publisher","first-page":"386","DOI":"10.1038\/sj.bjp.0707414","volume":"152","author":"V Yarotskyy","year":"2007","unstructured":"Yarotskyy, V. & Elmslie, K. S. Roscovitine, a cyclin-dependent kinase inhibitor, affects several gating mechanisms to inhibit cardiac L-type (Ca(V)1.2) calcium channels. Br. J. Pharmacol. 152, 386\u2013395 (2007)","journal-title":"Br. J. Pharmacol"},{"key":"BFnature22330_CR29","first-page":"486","volume":"15","author":"S Zechel","year":"2014","unstructured":"Zechel, S., Zajac, P., L\u00f6nnerberg, P., Ib\u00e1\u00f1ez, C. F. & Linnarsson, S. Topographical transcriptome mapping of the mouse medial ganglionic eminence by spatially resolved RNA-seq. Genome Biol. 15, 486 (2014)","journal-title":"Genome Biol."},{"key":"BFnature22330_CR30","doi-asserted-by":"publisher","first-page":"2306","DOI":"10.1093\/cercor\/bhm258","volume":"18","author":"R Batista-Brito","year":"2008","unstructured":"Batista-Brito, R., Machold, R., Klein, C. & Fishell, G. Gene expression in cortical interneuron precursors is prescient of their mature function. Cereb. Cortex 18, 2306\u20132317 (2008)","journal-title":"Cereb. Cortex"},{"key":"BFnature22330_CR31","doi-asserted-by":"publisher","first-page":"19038","DOI":"10.1073\/pnas.1013494107","volume":"107","author":"L Mao","year":"2010","unstructured":"Mao, L., Takamiya, K., Thomas, G., Lin, D. T. & Huganir, R. L. GRIP1 and 2 regulate activity-dependent AMPA receptor recycling via exocyst complex interactions. Proc. Natl Acad. Sci. USA 107, 19038\u201319043 (2010)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature22330_CR32","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1038\/nature17187","volume":"531","author":"AR Mardinly","year":"2016","unstructured":"Mardinly, A. R. et al. Sensory experience regulates cortical inhibition by inducing IGF1 in VIP neurons. Nature 531, 371\u2013375 (2016)","journal-title":"Nature"},{"key":"BFnature22330_CR33","doi-asserted-by":"publisher","first-page":"1561","DOI":"10.1002\/ajmg.a.35941","volume":"161","author":"M Lacaria","year":"2013","unstructured":"Lacaria, M., Gu, W. & Lupski, J. R. Circadian abnormalities in mouse models of Smith\u2013Magenis syndrome: evidence for involvement of RAI1. Am. J. Med. Genet. A 161, 1561\u20131568 (2013)","journal-title":"Am. J. Med. Genet. A"},{"key":"BFnature22330_CR34","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1093\/schbul\/sbq035","volume":"36","author":"DJ Blake","year":"2010","unstructured":"Blake, D. J. et al. TCF4, schizophrenia, and Pitt\u2013Hopkins syndrome. Schizophr. Bull. 36, 443\u2013447 (2010)","journal-title":"Schizophr. Bull."},{"key":"BFnature22330_CR35","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1038\/nature12517","volume":"501","author":"MA Lancaster","year":"2013","unstructured":"Lancaster, M. A. et al. Cerebral organoids model human brain development and microcephaly. Nature 501, 373\u2013379 (2013)","journal-title":"Nature"},{"key":"BFnature22330_CR36","doi-asserted-by":"publisher","first-page":"12770","DOI":"10.1073\/pnas.1202944109","volume":"109","author":"J Mariani","year":"2012","unstructured":"Mariani, J. et al. Modeling human cortical development in vitro using induced pluripotent stem cells. Proc. Natl Acad. Sci. USA 109, 12770\u201312775 (2012)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature22330_CR37","doi-asserted-by":"publisher","first-page":"20284","DOI":"10.1073\/pnas.1315710110","volume":"110","author":"T Kadoshima","year":"2013","unstructured":"Kadoshima, T. et al. Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex. Proc. Natl Acad. Sci. USA 110, 20284\u201320289 (2013)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature22330_CR38","doi-asserted-by":"publisher","first-page":"1238","DOI":"10.1016\/j.cell.2016.04.032","volume":"165","author":"X Qian","year":"2016","unstructured":"Qian, X. et al. Brain-region-specific organoids using mini-bioreactors for modeling ZIKV exposure. Cell 165, 1238\u20131254 (2016)","journal-title":"Cell"},{"key":"BFnature22330_CR39","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1038\/nn.3307","volume":"16","author":"JF Krey","year":"2013","unstructured":"Krey, J. F. et al. Timothy syndrome is associated with activity-dependent dendritic retraction in rodent and human neurons. Nat. Neurosci. 16, 201\u2013209 (2013)","journal-title":"Nat. Neurosci."},{"key":"BFnature22330_CR40","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1186\/s13073-014-0075-5","volume":"6","author":"Y Tian","year":"2014","unstructured":"Tian, Y. et al. Alteration in basal and depolarization induced transcriptional network in iPSC derived neurons from Timothy syndrome. Genome Med. 6, 75 (2014)","journal-title":"Genome Med."},{"key":"BFnature22330_CR41","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1038\/nature09855","volume":"471","author":"M Yazawa","year":"2011","unstructured":"Yazawa, M. et al. Using induced pluripotent stem cells to investigate cardiac phenotypes in Timothy syndrome. Nature 471, 230\u2013234 (2011)","journal-title":"Nature"},{"key":"BFnature22330_CR42","doi-asserted-by":"publisher","first-page":"e07103","DOI":"10.7554\/eLife.07103","volume":"4","author":"I Gallego Romero","year":"2015","unstructured":"Gallego Romero, I. et al. A panel of induced pluripotent stem cells from chimpanzees: a resource for comparative functional genomics. eLife 4, e07103 (2015)","journal-title":"eLife"},{"key":"BFnature22330_CR43","doi-asserted-by":"publisher","unstructured":"Birey, F ., Andersen, J. & Pas\u00b8ca, S. P. Generation and assembly of forebrain spheroids from human pluripotent stem cells. Protoc. Exch. http:\/\/dx.doi.org\/10.1038\/protex.2017.044 (2017)","DOI":"10.1038\/protex.2017.044"},{"key":"BFnature22330_CR44","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1038\/nbt.3440","volume":"34","author":"BE Deverman","year":"2016","unstructured":"Deverman, B. E. et al. Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nat. Biotechnol. 34, 204\u2013209 (2016)","journal-title":"Nat. Biotechnol."},{"key":"BFnature22330_CR45","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.neuron.2015.11.013","volume":"89","author":"Y Zhang","year":"2016","unstructured":"Zhang, Y. et al. Purification and characterization of progenitor and mature human astrocytes reveals transcriptional and functional differences with mouse. Neuron 89, 37\u201353 (2016)","journal-title":"Neuron"},{"key":"BFnature22330_CR46","doi-asserted-by":"crossref","unstructured":"Venkataramanappa, S., Simon, R. & Britsch, S. Ex utero electroporation and organotypic slice culture of mouse hippocampal tissue. J. Vis. Exp. (97), e52550 (2015)","DOI":"10.3791\/52550"},{"key":"BFnature22330_CR47","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1073\/pnas.2235688100","volume":"101","author":"T Matsuda","year":"2004","unstructured":"Matsuda, T. & Cepko, C. L. Electroporation and RNA interference in the rodent retina in vivo and in vitro. Proc. Natl Acad. Sci. USA 101, 16\u201322 (2004)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature22330_CR48","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1038\/nature13973","volume":"515","author":"JH Lui","year":"2014","unstructured":"Lui, J. H. et al. Radial glia require PDGFD\u2013PDGFR\u03b2 signalling in human but not mouse neocortex. Nature 515, 264\u2013268 (2014)","journal-title":"Nature"},{"key":"BFnature22330_CR49","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1016\/j.cell.2014.10.010","volume":"159","author":"N Renier","year":"2014","unstructured":"Renier, N. et al. iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging. Cell 159, 896\u2013910 (2014)","journal-title":"Cell"},{"key":"BFnature22330_CR50","doi-asserted-by":"publisher","first-page":"1202","DOI":"10.1016\/j.cell.2015.05.002","volume":"161","author":"EZ Macosko","year":"2015","unstructured":"Macosko, E. Z. et al. Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets. Cell 161, 1202\u20131214 (2015)","journal-title":"Cell"},{"key":"BFnature22330_CR51","doi-asserted-by":"publisher","first-page":"R106","DOI":"10.1186\/gb-2010-11-10-r106","volume":"11","author":"S Anders","year":"2010","unstructured":"Anders, S. & Huber, W. Differential expression analysis for sequence count data. Genome Biol. 11, R106 (2010)","journal-title":"Genome Biol."},{"key":"BFnature22330_CR52","doi-asserted-by":"publisher","first-page":"1096","DOI":"10.1038\/nmeth.2639","volume":"10","author":"S Picelli","year":"2013","unstructured":"Picelli, S. et al. Smart-seq2 for sensitive full-length transcriptome profiling in single cells. Nat. Methods 10, 1096\u20131098 (2013)","journal-title":"Nat. Methods"},{"key":"BFnature22330_CR53","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1038\/nmeth.2645","volume":"10","author":"P Brennecke","year":"2013","unstructured":"Brennecke, P. et al. Accounting for technical noise in single-cell RNA-seq experiments. Nat. Methods 10, 1093\u20131095 (2013)","journal-title":"Nat. Methods"},{"key":"BFnature22330_CR54","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1038\/nbt.3192","volume":"33","author":"R Satija","year":"2015","unstructured":"Satija, R., Farrell, J. A., Gennert, D., Schier, A. F. & Regev, A. Spatial reconstruction of single-cell gene expression data. Nat. Biotechnol. 33, 495\u2013502 (2015)","journal-title":"Nat. Biotechnol."},{"key":"BFnature22330_CR55","doi-asserted-by":"publisher","first-page":"639","DOI":"10.1016\/j.neuron.2010.09.024","volume":"68","author":"KD Micheva","year":"2010","unstructured":"Micheva, K. D., Busse, B., Weiler, N. C., O\u2019Rourke, N. & Smith, S. J. Single-synapse analysis of a diverse synapse population: proteomic imaging methods and markers. Neuron 68, 639\u2013653 (2010)","journal-title":"Neuron"},{"key":"BFnature22330_CR56","doi-asserted-by":"publisher","first-page":"399","DOI":"10.4196\/kjpp.2010.14.6.399","volume":"14","author":"HJ Jang","year":"2010","unstructured":"Jang, H. J. et al. The development of phasic and tonic inhibition in the rat visual cortex. Korean J. Physiol. Pharmacol. 14, 399\u2013405 (2010)","journal-title":"Korean J. Physiol. Pharmacol."}],"container-title":["Nature"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/nature22330.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/nature22330","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/doifinder\/10.1038\/nature22330","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/www.nature.com\/articles\/nature22330.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T10:24:45Z","timestamp":1766139885000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nature22330"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,26]]},"references-count":56,"journal-issue":{"issue":"7652","published-print":{"date-parts":[[2017,5,4]]}},"alternative-id":["BFnature22330"],"URL":"https:\/\/doi.org\/10.1038\/nature22330","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.727555406.793544342","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.727555406.793533733","asserted-by":"object"}]},"ISSN":["0028-0836","1476-4687"],"issn-type":[{"value":"0028-0836","type":"print"},{"value":"1476-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,26]]},"assertion":[{"value":"8 December 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 April 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 April 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Stanford University has filed a provisional patent application that covers the generation of region-specific neural spheroids and their assembly for studying human development and disease (US patent application number 62\/477,858). H.C.F. and K.R.C.M. were employees of BD Genomics during this study.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}