{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:00:55Z","timestamp":1791939655197,"version":"4.3.4"},"reference-count":35,"publisher":"Springer Science and Business Media LLC","issue":"7678","license":[{"start":{"date-parts":[[2017,9,25]],"date-time":"2017-09-25T00:00:00Z","timestamp":1506297600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nature"],"published-print":{"date-parts":[[2017,11]]},"DOI":"10.1038\/nature24280","type":"journal-article","created":{"date-parts":[[2017,9,22]],"date-time":"2017-09-22T11:35:35Z","timestamp":1506080135000},"page":"95-99","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":241,"title":["Single-cell RNA sequencing reveals a signature of sexual commitment in malaria parasites"],"prefix":"10.1038","volume":"551","author":[{"given":"Asaf","family":"Poran","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"N\u00f6tzel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar","family":"Aly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuria","family":"Mencia-Trinchant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal T.","family":"Harris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica L.","family":"Guzman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duane C.","family":"Hassane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Elemento","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bj\u00f6rn F. C.","family":"Kafsack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,9,25]]},"reference":[{"key":"BFnature24280_CR1","doi-asserted-by":"publisher","first-page":"610","DOI":"10.1016\/j.cell.2016.07.055","volume":"167","author":"AF Cowman","year":"2016","unstructured":"Cowman, A. F., Healer, J., Marapana, D. & Marsh, K. Malaria: biology and disease. Cell 167, 610\u2013624 (2016)","journal-title":"Cell"},{"key":"BFnature24280_CR2","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1017\/S0031182099006691","volume":"121","author":"F Silvestrini","year":"2000","unstructured":"Silvestrini, F., Alano, P. & Williams, J. L. Commitment to the production of male and female gametocytes in the human malaria parasite Plasmodium falciparum. Parasitology 121, 465\u2013471 (2000)","journal-title":"Parasitology"},{"key":"BFnature24280_CR3","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1017\/S0031182000061199","volume":"100","author":"MC Bruce","year":"1990","unstructured":"Bruce, M. C., Alano, P., Duthie, S. & Carter, R. Commitment of the malaria parasite Plasmodium falciparum to sexual and asexual development. Parasitology 100, 191\u2013200 (1990)","journal-title":"Parasitology"},{"key":"BFnature24280_CR4","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1038\/nature12920","volume":"507","author":"BFC Kafsack","year":"2014","unstructured":"Kafsack, B. F. C. et al. A transcriptional switch underlies commitment to sexual development in malaria parasites. Nature 507, 248\u2013252 (2014)","journal-title":"Nature"},{"key":"BFnature24280_CR5","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1038\/nature12970","volume":"507","author":"A Sinha","year":"2014","unstructured":"Sinha, A. et al. A cascade of DNA-binding proteins for sexual commitment and development in Plasmodium. Nature 507, 253\u2013257 (2014)","journal-title":"Nature"},{"key":"BFnature24280_CR6","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/j.chom.2014.07.004","volume":"16","author":"NMB Brancucci","year":"2014","unstructured":"Brancucci, N. M. B. et al. Heterochromatin protein 1 secures survival and transmission of malaria parasites. Cell Host Microbe 16, 165\u2013176 (2014)","journal-title":"Cell Host Microbe"},{"key":"BFnature24280_CR7","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/j.chom.2014.06.014","volume":"16","author":"BI Coleman","year":"2014","unstructured":"Coleman, B. I. et al. A Plasmodium falciparum histone deacetylase regulates antigenic variation and gametocyte conversion. Cell Host Microbe 16, 177\u2013186 (2014)","journal-title":"Cell Host Microbe"},{"key":"BFnature24280_CR8","doi-asserted-by":"publisher","first-page":"1618","DOI":"10.1111\/cmi.12460","volume":"17","author":"KG Pell\u00e9","year":"2015","unstructured":"Pell\u00e9, K. G. et al. Shared elements of host-targeting pathways among apicomplexan parasites of differing lifestyles. Cell. Microbiol. 17, 1618\u20131639 (2015)","journal-title":"Cell. Microbiol."},{"key":"BFnature24280_CR9","doi-asserted-by":"publisher","first-page":"1202","DOI":"10.1016\/j.cell.2015.05.002","volume":"161","author":"EZ Macosko","year":"2015","unstructured":"Macosko, E. Z. et al. Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets. Cell 161, 1202\u20131214 (2015)","journal-title":"Cell"},{"key":"BFnature24280_CR10","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1128\/EC.00340-08","volume":"8","author":"JS Sims","year":"2009","unstructured":"Sims, J. S. et al. Patterns of gene-specific and total transcriptional activity during the Plasmodium falciparum intraerythrocytic developmental cycle. Eukaryot. Cell 8, 327\u2013338 (2009)","journal-title":"Eukaryot. Cell"},{"key":"BFnature24280_CR11","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.molbiopara.2007.04.008","volume":"154","author":"QL Fivelman","year":"2007","unstructured":"Fivelman, Q. L. et al. Improved synchronous production of Plasmodium falciparum gametocytes in vitro. Mol. Biochem. Parasitol. 154, 119\u2013123 (2007)","journal-title":"Mol. Biochem. Parasitol."},{"key":"BFnature24280_CR12","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1038\/nbt.3192","volume":"33","author":"R Satija","year":"2015","unstructured":"Satija, R., Farrell, J. A., Gennert, D., Schier, A. F. & Regev, A. Spatial reconstruction of single-cell gene expression data. Nat. Biotechnol. 33, 495\u2013502 (2015)","journal-title":"Nat. Biotechnol."},{"key":"BFnature24280_CR13","doi-asserted-by":"publisher","first-page":"2110","DOI":"10.1093\/nar\/gkv1214","volume":"44","author":"PR Kensche","year":"2016","unstructured":"Kensche, P. R. et al. The nucleosome landscape of Plasmodium falciparum reveals chromatin architecture and dynamics of regulatory sequences. Nucleic Acids Res. 44, 2110\u20132124 (2016)","journal-title":"Nucleic Acids Res."},{"key":"BFnature24280_CR14","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1038\/nmeth.4150","volume":"14","author":"X Qiu","year":"2017","unstructured":"Qiu, X. et al. Single-cell mRNA quantification and differential analysis with Census. Nat. Methods 14, 309\u2013315 (2017)","journal-title":"Nat. Methods"},{"key":"BFnature24280_CR15","doi-asserted-by":"publisher","first-page":"910","DOI":"10.1126\/science.1188191","volume":"328","author":"JD Dvorin","year":"2010","unstructured":"Dvorin, J. D. et al. A plant-like kinase in Plasmodium falciparum regulates parasite egress from erythrocytes. Science 328, 910\u2013912 (2010)","journal-title":"Science"},{"key":"BFnature24280_CR16","doi-asserted-by":"publisher","first-page":"e5","DOI":"10.1371\/journal.pbio.0000005","volume":"1","author":"Z Bozdech","year":"2003","unstructured":"Bozdech, Z. et al. The transcriptome of the intraerythrocytic developmental cycle of Plasmodium falciparum. PLoS Biol. 1, e5 (2003)","journal-title":"PLoS Biol."},{"key":"BFnature24280_CR17","doi-asserted-by":"publisher","first-page":"41303","DOI":"10.1038\/srep41303","volume":"7","author":"A Claessens","year":"2017","unstructured":"Claessens, A., Affara, M., Assefa, S. A., Kwiatkowski, D. P. & Conway, D. J. Culture adaptation of malaria parasites selects for convergent loss-of-function mutants. Sci. Rep. 7, 41303 (2017)","journal-title":"Sci. Rep."},{"key":"BFnature24280_CR18","doi-asserted-by":"publisher","first-page":"490","DOI":"10.1016\/j.cell.2013.07.011","volume":"154","author":"GJ Narlikar","year":"2013","unstructured":"Narlikar, G. J., Sundaramoorthy, R. & Owen-Hughes, T. Mechanisms and functions of ATP-dependent chromatin-remodeling enzymes. Cell 154, 490\u2013503 (2013)","journal-title":"Cell"},{"key":"BFnature24280_CR19","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/j.ijpara.2009.09.002","volume":"40","author":"J Volz","year":"2010","unstructured":"Volz, J. et al. Potential epigenetic regulatory proteins localise to distinct nuclear sub-compartments in Plasmodium falciparum. Int. J. Parasitol. 40, 109\u2013121 (2010)","journal-title":"Int. J. Parasitol."},{"key":"BFnature24280_CR20","doi-asserted-by":"publisher","first-page":"976","DOI":"10.1016\/j.celrep.2012.08.038","volume":"2","author":"BO Park","year":"2012","unstructured":"Park, B. O., Ahrends, R. & Teruel, M. N. Consecutive positive feedback loops create a bistable switch that controls preadipocyte-to-adipocyte conversion. Cell Rep. 2, 976\u2013990 (2012)","journal-title":"Cell Rep."},{"key":"BFnature24280_CR21","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1016\/S0968-0004(99)01473-5","volume":"24","author":"M Laurent","year":"1999","unstructured":"Laurent, M. & Kellershohn, N. Multistability: a major means of differentiation and evolution in biological systems. Trends Biochem. Sci. 24, 418\u2013422 (1999)","journal-title":"Trends Biochem. Sci."},{"key":"BFnature24280_CR22","doi-asserted-by":"publisher","first-page":"e123","DOI":"10.1371\/journal.pcbi.0020123","volume":"2","author":"V Chickarmane","year":"2006","unstructured":"Chickarmane, V., Troein, C., Nuber, U. A., Sauro, H. M. & Peterson, C. Transcriptional dynamics of the embryonic stem cell switch. PLoS Comput. Biol. 2, e123 (2006)","journal-title":"PLoS Comput. Biol."},{"key":"BFnature24280_CR23","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1093\/toxsci\/kfq035","volume":"115","author":"S Bhattacharya","year":"2010","unstructured":"Bhattacharya, S. et al. A bistable switch underlying B-cell differentiation and its disruption by the environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin. Toxicol. Sci. 115, 51\u201365 (2010)","journal-title":"Toxicol. Sci."},{"key":"BFnature24280_CR24","doi-asserted-by":"publisher","first-page":"718","DOI":"10.1111\/cmi.12115","volume":"15","author":"J Guizetti","year":"2013","unstructured":"Guizetti, J. & Scherf, A. Silence, activate, poise and switch! Mechanisms of antigenic variation in Plasmodium falciparum. Cell. Microbiol. 15, 718\u2013726 (2013)","journal-title":"Cell. Microbiol."},{"key":"BFnature24280_CR25","doi-asserted-by":"publisher","first-page":"3156","DOI":"10.1128\/AAC.00197-15","volume":"59","author":"A Hott","year":"2015","unstructured":"Hott, A. et al. Artemisinin-resistant Plasmodium falciparum parasites exhibit altered patterns of development in infected erythrocytes. Antimicrob. Agents Chemother. 59, 3156\u20133167 (2015)","journal-title":"Antimicrob. Agents Chemother."},{"key":"BFnature24280_CR26","doi-asserted-by":"publisher","first-page":"e22","DOI":"10.1371\/journal.ppat.0020022","volume":"2","author":"R Dzikowski","year":"2006","unstructured":"Dzikowski, R., Frank, M. & Deitsch, K. Mutually exclusive expression of virulence genes by malaria parasites is regulated independently of antigen production. PLoS Pathog. 2, e22 (2006)","journal-title":"PLoS Pathog."},{"key":"BFnature24280_CR27","unstructured":"Moll, K., Ljungstr\u00f6m, I., Perlmann, H. & Scherf, A. (eds) Methods in Malaria Research 5th edn (MR4\/ATCC, 2008)"},{"key":"BFnature24280_CR28","doi-asserted-by":"publisher","first-page":"1007","DOI":"10.1038\/nmeth1132","volume":"4","author":"CM Armstrong","year":"2007","unstructured":"Armstrong, C. M. & Goldberg, D. E. An FKBP destabilization domain modulates protein levels in Plasmodium falciparum. Nat. Methods 4, 1007\u20131009 (2007)","journal-title":"Nat. Methods"},{"key":"BFnature24280_CR29","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak, K. J & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394Ct method. Methods 25, 402\u2013408 (2001)","journal-title":"Methods"},{"key":"BFnature24280_CR30","doi-asserted-by":"publisher","first-page":"R26","DOI":"10.1186\/gb-2005-6-3-r26","volume":"6","author":"RE Martin","year":"2005","unstructured":"Martin, R. E., Henry, R. I., Abbey, J. L., Clements, J. D. & Kirk, K. The \u2018permeome\u2019 of the malaria parasite: an overview of the membrane transport proteins of Plasmodium falciparum. Genome Biol. 6, R26 (2005)","journal-title":"Genome Biol."},{"key":"BFnature24280_CR31","first-page":"2579","volume":"9","author":"LVD Maaten","year":"2008","unstructured":"Maaten, L. V. D. & Hinton, G. Visualizing data using t-SNE. J. Mach. Learn. Res. 9, 2579\u20132605 (2008)","journal-title":"J. Mach. Learn. Res."},{"key":"BFnature24280_CR32","unstructured":"Csardi, G. & Nepusz, T. The igraph software package for complex network research. InterJournal \n                    http:\/\/www.interjournal.org\/manuscript_abstract.php?361100992\n                    \n                   (2006)"},{"key":"BFnature24280_CR33","doi-asserted-by":"publisher","first-page":"2347","DOI":"10.1093\/bioinformatics\/btq430","volume":"26","author":"CT Lopes","year":"2010","unstructured":"Lopes, C. T. et al. Cytoscape Web: an interactive web-based network browser. Bioinformatics 26, 2347\u20132348 (2010)","journal-title":"Bioinformatics"},{"key":"BFnature24280_CR34","doi-asserted-by":"publisher","first-page":"939","DOI":"10.1038\/nbt1098-939","volume":"16","author":"FP Roth","year":"1998","unstructured":"Roth, F. P., Hughes, J. D., Estep, P. W. & Church, G. M. Finding DNA regulatory motifs within unaligned noncoding sequences clustered by whole-genome mRNA quantitation. Nat. Biotechnol. 16, 939\u2013945 (1998)","journal-title":"Nat. Biotechnol."},{"key":"BFnature24280_CR35","doi-asserted-by":"publisher","first-page":"e1001165","DOI":"10.1371\/journal.ppat.1001165","volume":"6","author":"TL Campbell","year":"2010","unstructured":"Campbell, T. L., De Silva, E. K., Olszewski, K. L., Elemento, O. & Llin\u00e1s, M. Identification and genome-wide prediction of DNA binding specificities for the ApiAP2 family of regulators from the malaria parasite. PLoS Pathog. 6, e1001165 (2010)","journal-title":"PLoS Pathog."}],"container-title":["Nature"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nature24280.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nature24280","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/doifinder\/10.1038\/nature24280","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/nature24280.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T14:06:58Z","timestamp":1684418818000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nature24280"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,25]]},"references-count":35,"journal-issue":{"issue":"7678","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["BFnature24280"],"URL":"https:\/\/doi.org\/10.1038\/nature24280","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.732067660.793543023","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.732067660.793540695","asserted-by":"object"}]},"ISSN":["0028-0836","1476-4687"],"issn-type":[{"value":"0028-0836","type":"print"},{"value":"1476-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,25]]},"assertion":[{"value":"3 March 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 September 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 September 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}