{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:57:10Z","timestamp":1790650630635,"version":"4.1.0"},"reference-count":45,"publisher":"Springer Science and Business Media LLC","issue":"7680","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nature"],"published-print":{"date-parts":[[2017,11]]},"DOI":"10.1038\/nature24490","type":"journal-article","created":{"date-parts":[[2017,11,7]],"date-time":"2017-11-07T13:36:26Z","timestamp":1510061786000},"page":"394-397","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":242,"title":["Structure and assembly of the Ebola virus nucleocapsid"],"prefix":"10.1038","volume":"551","author":[{"given":"William","family":"Wan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa","family":"Kolesnikova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mairi","family":"Clarke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Koehler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Noda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Becker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A. G.","family":"Briggs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,11,8]]},"reference":[{"key":"BFnature24490_CR1","unstructured":"Feldmann, H., Sanchez, A. & Geisbert, T. in Fields Virology (eds Knipe, D. M. & Howley, P. M. ) 923\u2013956 (Lippincott Williams & Wilkins, 2013)"},{"key":"BFnature24490_CR2","unstructured":"Lamb, R. A. in Fields Virology (eds Knipe, D. M. & Howley, P. M. ) 880\u2013884 (Lippincott Williams & Wilkins, 2013)"},{"key":"BFnature24490_CR3","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1126\/science.1125280","volume":"313","author":"AAV Albertini","year":"2006","unstructured":"Albertini, A. A. V. et al. Crystal structure of the rabies virus nucleoprotein-RNA complex. Science 313, 360\u2013363 (2006)","journal-title":"Science"},{"key":"BFnature24490_CR4","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1126\/science.1126953","volume":"313","author":"TJ Green","year":"2006","unstructured":"Green, T. J., Zhang, X., Wertz, G. W. & Luo, M. Structure of the vesicular stomatitis virus nucleoprotein-RNA complex. Science 313, 357\u2013360 (2006)","journal-title":"Science"},{"key":"BFnature24490_CR5","doi-asserted-by":"publisher","first-page":"1279","DOI":"10.1126\/science.1177634","volume":"326","author":"RG Tawar","year":"2009","unstructured":"Tawar, R. G. et al. Crystal structure of a nucleocapsid-like nucleoprotein-RNA complex of respiratory syncytial virus. Science 326, 1279\u20131283 (2009)","journal-title":"Science"},{"key":"BFnature24490_CR6","doi-asserted-by":"publisher","first-page":"E1792","DOI":"10.1073\/pnas.1503941112","volume":"112","author":"M Alayyoubi","year":"2015","unstructured":"Alayyoubi, M., Leser, G. P., Kors, C. A. & Lamb, R. A. Structure of the paramyxovirus parainfluenza virus 5 nucleoprotein-RNA complex. Proc. Natl Acad. Sci. USA 112, E1792\u2013E1799 (2015)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature24490_CR7","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1126\/science.1181766","volume":"327","author":"P Ge","year":"2010","unstructured":"Ge, P. et al. Cryo-EM model of the bullet-shaped vesicular stomatitis virus. Science 327, 689\u2013693 (2010)","journal-title":"Science"},{"key":"BFnature24490_CR8","doi-asserted-by":"publisher","first-page":"15208","DOI":"10.1073\/pnas.1413268111","volume":"111","author":"R Cox","year":"2014","unstructured":"Cox, R. et al. Structural studies on the authentic mumps virus nucleocapsid showing uncoiling by the phosphoprotein. Proc. Natl Acad. Sci. USA 111, 15208\u201315213 (2014)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature24490_CR9","doi-asserted-by":"publisher","first-page":"704","DOI":"10.1126\/science.aaa5137","volume":"348","author":"I Gutsche","year":"2015","unstructured":"Gutsche, I. et al. Near-atomic cryo-EM structure of the helical measles virus nucleocapsid. Science 348, 704\u2013707 (2015)","journal-title":"Science"},{"key":"BFnature24490_CR10","doi-asserted-by":"publisher","first-page":"e1001196","DOI":"10.1371\/journal.pbio.1001196","volume":"9","author":"TAM Bharat","year":"2011","unstructured":"Bharat, T. A. M. et al. Cryo-electron tomography of Marburg virus particles and their morphogenesis within infected cells. PLoS Biol. 9, e1001196 (2011)","journal-title":"PLoS Biol."},{"key":"BFnature24490_CR11","doi-asserted-by":"publisher","first-page":"4275","DOI":"10.1073\/pnas.1120453109","volume":"109","author":"TAM Bharat","year":"2012","unstructured":"Bharat, T. A. M. et al. Structural dissection of Ebola virus and its assembly determinants using cryo-electron tomography. Proc. Natl Acad. Sci. USA 109, 4275\u20134280 (2012)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature24490_CR12","doi-asserted-by":"publisher","first-page":"e29608","DOI":"10.1371\/journal.pone.0029608","volume":"7","author":"DR Beniac","year":"2012","unstructured":"Beniac, D. R. et al. The organisation of Ebola virus reveals a capacity for extensive, modular polyploidy. PLoS ONE 7, e29608 (2012)","journal-title":"PLoS ONE"},{"key":"BFnature24490_CR13","doi-asserted-by":"publisher","first-page":"1734","DOI":"10.1099\/vir.0.053025-0","volume":"94","author":"SE Bakker","year":"2013","unstructured":"Bakker, S. E. et al. The respiratory syncytial virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid. J. Gen. Virol. 94, 1734\u20131738 (2013)","journal-title":"J. Gen. Virol."},{"key":"BFnature24490_CR14","doi-asserted-by":"publisher","first-page":"1478","DOI":"10.1099\/vir.0.019794-0","volume":"91","author":"T Noda","year":"2010","unstructured":"Noda, T., Hagiwara, K., Sagara, H. & Kawaoka, Y. Characterization of the Ebola virus nucleoprotein-RNA complex. J. Gen. Virol. 91, 1478\u20131483 (2010)","journal-title":"J. Gen. Virol."},{"key":"BFnature24490_CR15","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1016\/S1097-2765(02)00588-9","volume":"10","author":"Y Huang","year":"2002","unstructured":"Huang, Y., Xu, L., Sun, Y. & Nabel, G. J. The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein. Mol. Cell 10, 307\u2013316 (2002)","journal-title":"Mol. Cell"},{"key":"BFnature24490_CR16","doi-asserted-by":"publisher","first-page":"2420","DOI":"10.1107\/S1399004714014710","volume":"70","author":"PJ Dziuban\u00b4\u2009ska","year":"2014","unstructured":"Dziuban\u00b4\u2009ska, P. J., Derewenda, U., Ellena, J. F., Engel, D. A. & Derewenda, Z. S. The structure of the C-terminal domain of the Zaire ebolavirus nucleoprotein. Acta Crystallogr. D 70, 2420\u20132429 (2014)","journal-title":"Acta Crystallogr. D"},{"key":"BFnature24490_CR17","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1016\/j.celrep.2015.03.034","volume":"11","author":"DW Leung","year":"2015","unstructured":"Leung, D. W. et al. An Intrinsically disordered peptide from Ebola virus VP35 controls viral RNA synthesis by modulating nucleoprotein-RNA interactions. Cell Reports 11, 376\u2013389 (2015)","journal-title":"Cell Reports"},{"key":"BFnature24490_CR18","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1007\/s13238-015-0163-3","volume":"6","author":"S Dong","year":"2015","unstructured":"Dong, S. et al. Insight into the Ebola virus nucleocapsid assembly mechanism: crystal structure of Ebola virus nucleoprotein core domain at 1.8 \u00c5 resolution. Protein Cell 6, 351\u2013362 (2015)","journal-title":"Protein Cell"},{"key":"BFnature24490_CR19","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1016\/j.celrep.2015.06.003","volume":"12","author":"RN Kirchdoerfer","year":"2015","unstructured":"Kirchdoerfer, R. N., Abelson, D. M., Li, S., Wood, M. R. & Saphire, E. O. Assembly of the Ebola virus nucleoprotein from a chaperoned VP35 complex. Cell Rep. 12, 140\u2013149 (2015)","journal-title":"Cell Rep."},{"key":"BFnature24490_CR20","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1016\/j.mib.2011.07.011","volume":"14","author":"RW Ruigrok","year":"2011","unstructured":"Ruigrok, R. W., Cr\u00e9pin, T. & Kolakofsky, D. Nucleoproteins and nucleocapsids of negative-strand RNA viruses. Curr. Opin. Microbiol. 14, 504\u2013510 (2011)","journal-title":"Curr. Opin. Microbiol."},{"key":"BFnature24490_CR21","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1292\/jvms.67.325","volume":"67","author":"T Noda","year":"2005","unstructured":"Noda, T., Aoyama, K., Sagara, H., Kida, H. & Kawaoka, Y. Nucleocapsid-like structures of Ebola virus reconstructed using electron tomography. J. Vet. Med. Sci. 67, 325\u2013328 (2005)","journal-title":"J. Vet. Med. Sci."},{"key":"BFnature24490_CR22","doi-asserted-by":"publisher","first-page":"440","DOI":"10.4161\/viru.21302","volume":"3","author":"APP Zhang","year":"2012","unstructured":"Zhang, A. P. P. et al. The ebolavirus VP24 interferon antagonist: know your enemy. Virulence 3, 440\u2013445 (2012)","journal-title":"Virulence"},{"key":"BFnature24490_CR23","doi-asserted-by":"publisher","first-page":"e1002550","DOI":"10.1371\/journal.ppat.1002550","volume":"8","author":"APP Zhang","year":"2012","unstructured":"Zhang, A. P. P. et al. The Ebola virus interferon antagonist VP24 directly binds STAT1 and has a novel, pyramidal fold. PLoS Pathog. 8, e1002550 (2012)","journal-title":"PLoS Pathog."},{"key":"BFnature24490_CR24","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1073\/pnas.0807854106","volume":"106","author":"DW Leung","year":"2009","unstructured":"Leung, D. W. et al. Structure of the Ebola VP35 interferon inhibitory domain. Proc. Natl Acad. Sci. USA 106, 411\u2013416 (2009)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature24490_CR25","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1038\/nsmb.1765","volume":"17","author":"DW Leung","year":"2010","unstructured":"Leung, D. W. et al. Structural basis for dsRNA recognition and interferon antagonism by Ebola VP35. Nat. Struct. Mol. Biol. 17, 165\u2013172 (2010)","journal-title":"Nat. Struct. Mol. Biol."},{"key":"BFnature24490_CR26","doi-asserted-by":"publisher","first-page":"506","DOI":"10.1126\/science.aaf9620","volume":"353","author":"FKM Schur","year":"2016","unstructured":"Schur, F. K. M. et al. An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation. Science 353, 506\u2013508 (2016)","journal-title":"Science"},{"key":"BFnature24490_CR27","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.jsb.2005.07.007","volume":"152","author":"DN Mastronarde","year":"2005","unstructured":"Mastronarde, D. N. Automated electron microscope tomography using robust prediction of specimen movements. J. Struct. Biol. 152, 36\u201351 (2005)","journal-title":"J. Struct. Biol."},{"key":"BFnature24490_CR28","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.jsb.2016.06.007","volume":"197","author":"WJH Hagen","year":"2017","unstructured":"Hagen, W. J. H., Wan, W. & Briggs, J. A. G. Implementation of a cryo-electron tomography tilt-scheme optimized for high resolution subtomogram averaging. J. Struct. Biol. 197, 191\u2013198 (2017)","journal-title":"J. Struct. Biol."},{"key":"BFnature24490_CR29","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1038\/nmeth.2472","volume":"10","author":"X Li","year":"2013","unstructured":"Li, X. et al. Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM. Nat. Methods 10, 584\u2013590 (2013)","journal-title":"Nat. Methods"},{"key":"BFnature24490_CR30","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1016\/j.jsb.2015.08.008","volume":"192","author":"A Rohou","year":"2015","unstructured":"Rohou, A. & Grigorieff, N. CTFFIND4: fast and accurate defocus estimation from electron micrographs. J. Struct. Biol. 192, 216\u2013221 (2015)","journal-title":"J. Struct. Biol."},{"key":"BFnature24490_CR31","doi-asserted-by":"publisher","first-page":"e06980","DOI":"10.7554\/eLife.06980","volume":"4","author":"T Grant","year":"2015","unstructured":"Grant, T. & Grigorieff, N. Measuring the optimal exposure for single particle cryo-EM using a 2.6 \u00c5 reconstruction of rotavirus VP6. eLife 4, e06980 (2015). vol no","journal-title":"eLife"},{"key":"BFnature24490_CR32","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1016\/j.jsb.2009.08.016","volume":"168","author":"Q Xiong","year":"2009","unstructured":"Xiong, Q., Morphew, M. K., Schwartz, C. L., Hoenger, A. H. & Mastronarde, D. N. CTF determination and correction for low dose tomographic tilt series. J. Struct. Biol. 168, 378\u2013387 (2009)","journal-title":"J. Struct. Biol."},{"key":"BFnature24490_CR33","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1006\/jsbi.1996.0013","volume":"116","author":"JR Kremer","year":"1996","unstructured":"Kremer, J. R., Mastronarde, D. N. & McIntosh, J. R. Computer visualization of three-dimensional image data using IMOD. J. Struct. Biol. 116, 71\u201376 (1996)","journal-title":"J. Struct. Biol."},{"key":"BFnature24490_CR34","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/j.jsb.2008.05.003","volume":"164","author":"S Pruggnaller","year":"2008","unstructured":"Pruggnaller, S., Mayr, M. & Frangakis, A. S. A visualization and segmentation toolbox for electron microscopy. J. Struct. Biol. 164, 161\u2013165 (2008)","journal-title":"J. Struct. Biol."},{"key":"BFnature24490_CR35","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1016\/j.jsb.2004.10.006","volume":"149","author":"S Nickell","year":"2005","unstructured":"Nickell, S. et al. TOM software toolbox: acquisition and analysis for electron tomography. J. Struct. Biol. 149, 227\u2013234 (2005)","journal-title":"J. Struct. Biol."},{"key":"BFnature24490_CR36","doi-asserted-by":"publisher","first-page":"4729","DOI":"10.1073\/pnas.0409178102","volume":"102","author":"F F\u00f6rster","year":"2005","unstructured":"F\u00f6rster, F., Medalia, O., Zauberman, N., Baumeister, W. & Fass, D. Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography. Proc. Natl Acad. Sci. USA 102, 4729\u20134734 (2005)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature24490_CR37","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.jsb.2011.12.017","volume":"178","author":"D Casta\u00f1o-D\u00edez","year":"2012","unstructured":"Casta\u00f1o-D\u00edez, D., Kudryashev, M., Arheit, M. & Stahlberg, H. Dynamo: a flexible, user-friendly development tool for subtomogram averaging of cryo-EM data in high-performance computing environments. J. Struct. Biol. 178, 139\u2013151 (2012)","journal-title":"J. Struct. Biol."},{"key":"BFnature24490_CR38","doi-asserted-by":"publisher","first-page":"14153","DOI":"10.1073\/pnas.172520299","volume":"99","author":"AS Frangakis","year":"2002","unstructured":"Frangakis, A. S. et al. Identification of macromolecular complexes in cryoelectron tomograms of phantom cells. Proc. Natl Acad. Sci. USA 99, 14153\u201314158 (2002)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature24490_CR39","doi-asserted-by":"publisher","first-page":"1605","DOI":"10.1002\/jcc.20084","volume":"25","author":"EF Pettersen","year":"2004","unstructured":"Pettersen, E. F. et al. UCSF Chimera\u2014a visualization system for exploratory research and analysis. J. Comput. Chem. 25, 1605\u20131612 (2004)","journal-title":"J. Comput. Chem."},{"key":"BFnature24490_CR40","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/j.ultramic.2013.06.004","volume":"135","author":"S Chen","year":"2013","unstructured":"Chen, S. et al. High-resolution noise substitution to measure overfitting and validate resolution in 3D structure determination by single particle electron cryomicroscopy. Ultramicroscopy 135, 24\u201335 (2013)","journal-title":"Ultramicroscopy"},{"key":"BFnature24490_CR41","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/j.jmb.2003.07.013","volume":"333","author":"PB Rosenthal","year":"2003","unstructured":"Rosenthal, P. B. & Henderson, R. Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy. J. Mol. Biol. 333, 721\u2013745 (2003)","journal-title":"J. Mol. Biol."},{"key":"BFnature24490_CR42","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1107\/S0907444910007493","volume":"66","author":"P Emsley","year":"2010","unstructured":"Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr. D 66, 486\u2013501 (2010)","journal-title":"Acta Crystallogr. D"},{"key":"BFnature24490_CR43","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1016\/j.ymeth.2009.04.005","volume":"49","author":"LG Trabuco","year":"2009","unstructured":"Trabuco, L. G., Villa, E., Schreiner, E., Harrison, C. B. & Schulten, K. Molecular dynamics flexible fitting: a practical guide to combine cryo-electron microscopy and X-ray crystallography. Methods 49, 174\u2013180 (2009)","journal-title":"Methods"},{"key":"BFnature24490_CR44","doi-asserted-by":"publisher","first-page":"1781","DOI":"10.1002\/jcc.20289","volume":"26","author":"JC Phillips","year":"2005","unstructured":"Phillips, J. C. et al. Scalable molecular dynamics with NAMD. J. Comput. Chem. 26, 1781\u20131802 (2005)","journal-title":"J. Comput. Chem."},{"key":"BFnature24490_CR45","doi-asserted-by":"publisher","first-page":"1017","DOI":"10.1016\/j.celrep.2014.01.043","volume":"6","author":"MR Edwards","year":"2014","unstructured":"Edwards, M. R. et al. The Marburg virus VP24 protein interacts with Keap1 to activate the cytoprotective antioxidant response pathway. Cell Rep. 6, 1017\u20131025 (2014)","journal-title":"Cell Rep."}],"container-title":["Nature"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nature24490.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nature24490","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/doifinder\/10.1038\/nature24490","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/nature24490.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T14:08:05Z","timestamp":1684418885000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nature24490"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11]]},"references-count":45,"journal-issue":{"issue":"7680","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["BFnature24490"],"URL":"https:\/\/doi.org\/10.1038\/nature24490","relation":{},"ISSN":["0028-0836","1476-4687"],"issn-type":[{"value":"0028-0836","type":"print"},{"value":"1476-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11]]},"assertion":[{"value":"8 June 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 October 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 November 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}