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Herein we report crystal structures of apo- and haemoglobin-bound HpuA, an essential component of this haem import system. The interface involves long loops on the bacterial receptor that present hydrophobic side chains for packing against the surface of haemoglobin. Interestingly, our structural and biochemical analyses of <jats:italic>Kingella denitrificans<\/jats:italic> and <jats:italic>Neisseria gonorrhoeae<\/jats:italic> HpuA mutants, although validating the interactions observed in the crystal structure, show how <jats:italic>Neisseriaceae<\/jats:italic> have the fascinating ability to diversify functional sequences and yet retain the haemoglobin binding function. Our results present the first description of HpuA\u2019s role in direct binding of haemoglobin.<\/jats:p>","DOI":"10.1038\/ncomms10172","type":"journal-article","created":{"date-parts":[[2015,12,16]],"date-time":"2015-12-16T11:38:00Z","timestamp":1450265880000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Structural analysis of haemoglobin binding by HpuA from the Neisseriaceae family"],"prefix":"10.1038","volume":"6","author":[{"given":"Chi T.","family":"Wong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingqi","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akshari","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A.","family":"Garnett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve J.","family":"Matthews","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen A.","family":"Hare","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,12,16]]},"reference":[{"key":"BFncomms10172_CR1","first-page":"1","volume":"80","author":"JJ Bullen","year":"1978","unstructured":"Bullen, J. 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