{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T16:46:06Z","timestamp":1790354766597,"version":"4.1.0"},"reference-count":70,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,3,18]],"date-time":"2016-03-18T00:00:00Z","timestamp":1458259200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,3,18]],"date-time":"2016-03-18T00:00:00Z","timestamp":1458259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Commun"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Numerical centrosome aberrations underlie certain developmental abnormalities and may promote cancer. A cell maintains normal centrosome numbers by coupling centrosome duplication with segregation, which is achieved through sustained association of each centrosome with a mitotic spindle pole. Although the microcephaly- and primordial dwarfism-linked centrosomal protein CEP215 has been implicated in this process, the molecular mechanism responsible remains unclear. Here, using proteomic profiling, we identify the minus end-directed microtubule motor protein HSET as a direct binding partner of CEP215. Targeted deletion of the HSET-binding domain of CEP215 in vertebrate cells causes centrosome detachment and results in HSET depletion at centrosomes, a phenotype also observed in CEP215-deficient patient-derived cells. Moreover, in cancer cells with centrosome amplification, the CEP215\u2013HSET complex promotes the clustering of extra centrosomes into pseudo-bipolar spindles, thereby ensuring viable cell division. Therefore, stabilization of the centrosome\u2013spindle pole interface by the CEP215\u2013HSET complex could promote survival of cancer cells containing supernumerary centrosomes.<\/jats:p>","DOI":"10.1038\/ncomms11005","type":"journal-article","created":{"date-parts":[[2016,3,18]],"date-time":"2016-03-18T11:26:27Z","timestamp":1458300387000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":92,"title":["A CEP215\u2013HSET complex links centrosomes with spindle poles and drives centrosome clustering in cancer"],"prefix":"10.1038","volume":"7","author":[{"given":"Pavithra L.","family":"Chavali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gayathri","family":"Chandrasekaran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexis R.","family":"Barr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e9ter","family":"T\u00e1trai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evaggelia K.","family":"Papachristou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Geoffrey","family":"Woods","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sreenivas","family":"Chavali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanni","family":"Gergely","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,3,18]]},"reference":[{"key":"BFncomms11005_CR1","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1083\/jcb.138.3.615","volume":"138","author":"R Heald","year":"1997","unstructured":"Heald, R., Tournebize, R., Habermann, A., Karsenti, E. & Hyman, A. Spindle assembly in Xenopus egg extracts: respective roles of centrosomes and microtubule self-organization. J. Cell Biol. 138, 615\u2013628 (1997).","journal-title":"J. Cell Biol."},{"key":"BFncomms11005_CR2","doi-asserted-by":"publisher","first-page":"1154","DOI":"10.1038\/ncb2345","volume":"13","author":"EA Nigg","year":"2011","unstructured":"Nigg, E. A. & Stearns, T. The centrosome cycle: centriole biogenesis, duplication and inherent asymmetries. Nat. Cell Biol. 13, 1154\u20131160 (2011).","journal-title":"Nat. Cell Biol."},{"key":"BFncomms11005_CR3","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1038\/nrm3373","volume":"13","author":"P Gonczy","year":"2012","unstructured":"Gonczy, P. Towards a molecular architecture of centriole assembly. Nat. Rev. Mol. Cell Biol. 13, 425\u2013435 (2012).","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"BFncomms11005_CR4","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1042\/BST20140269","volume":"43","author":"PL Chavali","year":"2015","unstructured":"Chavali, P. L., Peset, I. & Gergely, F. Centrosomes and mitotic spindle poles: a recent liaison? Biochem. Soc. Trans. 43, 13\u201318 (2015).","journal-title":"Biochem. Soc. Trans."},{"key":"BFncomms11005_CR5","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/S0960-9822(99)00276-6","volume":"10","author":"A Khodjakov","year":"2000","unstructured":"Khodjakov, A., Cole, R. W., Oakley, B. R. & Rieder, C. L. Centrosome-independent mitotic spindle formation in vertebrates. Curr. Biol. 10, 59\u201367 (2000).","journal-title":"Curr. Biol."},{"key":"BFncomms11005_CR6","doi-asserted-by":"publisher","first-page":"1375","DOI":"10.1016\/j.cell.2006.05.025","volume":"125","author":"R Basto","year":"2006","unstructured":"Basto, R. et al. Flies without centrioles. Cell 125, 1375\u20131386 (2006).","journal-title":"Cell"},{"key":"BFncomms11005_CR7","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1083\/jcb.201309038","volume":"203","author":"JH Sir","year":"2013","unstructured":"Sir, J. H. et al. Loss of centrioles causes chromosomal instability in vertebrate somatic cells. J. Cell Biol. 203, 747\u2013756 (2013).","journal-title":"J. Cell Biol."},{"key":"BFncomms11005_CR8","doi-asserted-by":"publisher","first-page":"831","DOI":"10.1083\/jcb.200407090","volume":"167","author":"H Maiato","year":"2004","unstructured":"Maiato, H., Rieder, C. L. & Khodjakov, A. Kinetochore-driven formation of kinetochore fibers contributes to spindle assembly during animal mitosis. J. Cell Biol. 167, 831\u2013840 (2004).","journal-title":"J. Cell Biol."},{"key":"BFncomms11005_CR9","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1083\/jcb.135.2.399","volume":"135","author":"T Gaglio","year":"1996","unstructured":"Gaglio, T. et al. Opposing motor activities are required for the organization of the mammalian mitotic spindle pole. J. Cell Biol. 135, 399\u2013414 (1996).","journal-title":"J. Cell Biol."},{"key":"BFncomms11005_CR10","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1083\/jcb.200505107","volume":"171","author":"G Goshima","year":"2005","unstructured":"Goshima, G., Nedelec, F. & Vale, R. D. Mechanisms for focusing mitotic spindle poles by minus end-directed motor proteins. J. Cell Biol. 171, 229\u2013240 (2005).","journal-title":"J. Cell Biol."},{"key":"BFncomms11005_CR11","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1038\/71338","volume":"2","author":"SJ King","year":"2000","unstructured":"King, S. J. & Schroer, T. A. Dynactin increases the processivity of the cytoplasmic dynein motor. Nat. Cell Biol. 2, 20\u201324 (2000).","journal-title":"Nat. Cell Biol."},{"key":"BFncomms11005_CR12","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1083\/jcb.149.4.851","volume":"149","author":"A Merdes","year":"2000","unstructured":"Merdes, A., Heald, R., Samejima, K., Earnshaw, W. C. & Cleveland, D. W. Formation of spindle poles by dynein\/dynactin-dependent transport of NuMA. J. Cell Biol. 149, 851\u2013862 (2000).","journal-title":"J. Cell Biol."},{"key":"BFncomms11005_CR13","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1083\/jcb.200810091","volume":"184","author":"AD Silk","year":"2009","unstructured":"Silk, A. D., Holland, A. J. & Cleveland, D. W. Requirements for NuMA in maintenance and establishment of mammalian spindle poles. J. Cell Biol. 184, 677\u2013690 (2009).","journal-title":"J. Cell Biol."},{"key":"BFncomms11005_CR14","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1242\/jcs.107.4.859","volume":"107","author":"SA Endow","year":"1994","unstructured":"Endow, S. A., Chandra, R., Komma, D. J., Yamamoto, A. H. & Salmon, E. D. Mutants of the Drosophila ncd microtubule motor protein cause centrosomal and spindle pole defects in mitosis. J. Cell Sci. 107, 859\u2013867 (1994).","journal-title":"J. Cell Sci."},{"key":"BFncomms11005_CR15","doi-asserted-by":"publisher","first-page":"1348","DOI":"10.1091\/mbc.e08-09-0971","volume":"20","author":"S Cai","year":"2009","unstructured":"Cai, S., Weaver, L. N., Ems-McClung, S. C. & Walczak, C. E. Kinesin-14 family proteins HSET\/XCTK2 control spindle length by cross-linking and sliding microtubules. Mol. Biol. Cell 20, 1348\u20131359 (2009).","journal-title":"Mol. Biol. Cell"},{"key":"BFncomms11005_CR16","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1083\/jcb.147.2.351","volume":"147","author":"V Mountain","year":"1999","unstructured":"Mountain, V. et al. The kinesin-related protein, HSET, opposes the activity of Eg5 and cross-links microtubules in the mammalian mitotic spindle. J. Cell Biol. 147, 351\u2013366 (1999).","journal-title":"J. Cell Biol."},{"key":"BFncomms11005_CR17","doi-asserted-by":"publisher","first-page":"5391","DOI":"10.1242\/jcs.107474","volume":"125","author":"J Kleylein-Sohn","year":"2012","unstructured":"Kleylein-Sohn, J. et al. Acentrosomal spindle organization renders cancer cells dependent on the kinesin HSET. J. Cell Sci. 125, 5391\u20135402 (2012).","journal-title":"J. Cell Sci."},{"key":"BFncomms11005_CR18","doi-asserted-by":"publisher","first-page":"2189","DOI":"10.1101\/gad.1700908","volume":"22","author":"M Kwon","year":"2008","unstructured":"Kwon, M. et al. Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes. Genes Dev. 22, 2189\u20132203 (2008).","journal-title":"Genes Dev."},{"key":"BFncomms11005_CR19","doi-asserted-by":"publisher","first-page":"1032","DOI":"10.1016\/j.cell.2008.05.039","volume":"133","author":"R Basto","year":"2008","unstructured":"Basto, R. et al. Centrosome amplification can initiate tumorigenesis in flies. Cell 133, 1032\u20131042 (2008).","journal-title":"Cell"},{"key":"BFncomms11005_CR20","doi-asserted-by":"publisher","first-page":"4037","DOI":"10.1091\/mbc.e07-05-0474","volume":"18","author":"J Zhang","year":"2007","unstructured":"Zhang, J. & Megraw, T. L. Proper recruitment of gamma-tubulin and D-TACC\/Msps to embryonic Drosophila centrosomes requires Centrosomin Motif 1. Mol. Biol. Cell 18, 4037\u20134049 (2007).","journal-title":"Mol. Biol. Cell"},{"key":"BFncomms11005_CR21","doi-asserted-by":"publisher","first-page":"1089","DOI":"10.1083\/jcb.201007030","volume":"191","author":"YK Choi","year":"2010","unstructured":"Choi, Y. K., Liu, P., Sze, S. K., Dai, C. & Qi, R. Z. CDK5RAP2 stimulates microtubule nucleation by the gamma-tubulin ring complex. J. Cell Biol. 191, 1089\u20131095 (2010).","journal-title":"J. Cell Biol."},{"key":"BFncomms11005_CR22","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1091\/mbc.e07-04-0371","volume":"19","author":"KW Fong","year":"2008","unstructured":"Fong, K. W., Choi, Y. K., Rattner, J. B. & Qi, R. Z. CDK5RAP2 is a pericentriolar protein that functions in centrosomal attachment of the gamma-tubulin ring complex. Mol. Biol. Cell 19, 115\u2013125 (2008).","journal-title":"Mol. Biol. Cell"},{"key":"BFncomms11005_CR23","doi-asserted-by":"publisher","first-page":"763","DOI":"10.1016\/j.cub.2004.03.042","volume":"14","author":"KE Sawin","year":"2004","unstructured":"Sawin, K. E., Lourenco, P. C. & Snaith, H. A. Microtubule nucleation at non-spindle pole body microtubule-organizing centers requires fission yeast centrosomin-related protein mod20p. Curr. Biol. 14, 763\u2013775 (2004).","journal-title":"Curr. Biol."},{"key":"BFncomms11005_CR24","doi-asserted-by":"publisher","first-page":"e5976","DOI":"10.1371\/journal.pone.0005976","volume":"4","author":"L Haren","year":"2009","unstructured":"Haren, L., Stearns, T. & Luders, J. Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components. PLoS ONE 4, e5976 (2009).","journal-title":"PLoS ONE"},{"key":"BFncomms11005_CR25","doi-asserted-by":"publisher","first-page":"4321","DOI":"10.1242\/jcs.020248","volume":"120","author":"S Graser","year":"2007","unstructured":"Graser, S., Stierhof, Y. D. & Nigg, E. A. Cep68 and Cep215 (Cdk5rap2) are required for centrosome cohesion. J. Cell Sci. 120, 4321\u20134331 (2007).","journal-title":"J. Cell Sci."},{"key":"BFncomms11005_CR26","doi-asserted-by":"publisher","first-page":"913","DOI":"10.1016\/j.devcel.2010.05.017","volume":"18","author":"JA Barrera","year":"2010","unstructured":"Barrera, J. A. et al. CDK5RAP2 regulates centriole engagement and cohesion in mice. Dev. Cell 18, 913\u2013926 (2010).","journal-title":"Dev. Cell"},{"key":"BFncomms11005_CR27","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1083\/jcb.200912163","volume":"189","author":"AR Barr","year":"2010","unstructured":"Barr, A. R., Kilmartin, J. V. & Gergely, F. CDK5RAP2 functions in centrosome to spindle pole attachment and DNA damage response. J. Cell Biol. 189, 23\u201339 (2010).","journal-title":"J. Cell Biol."},{"key":"BFncomms11005_CR28","doi-asserted-by":"publisher","first-page":"1148","DOI":"10.1038\/ncb2591","volume":"14","author":"S Lawo","year":"2012","unstructured":"Lawo, S., Hasegan, M., Gupta, G. D. & Pelletier, L. Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material. Nat. Cell Biol. 14, 1148\u20131158 (2012).","journal-title":"Nat. Cell Biol."},{"key":"BFncomms11005_CR29","doi-asserted-by":"publisher","first-page":"386","DOI":"10.1016\/j.neuron.2010.03.036","volume":"66","author":"JJ Buchman","year":"2010","unstructured":"Buchman, J. J. et al. Cdk5rap2 interacts with pericentrin to maintain the neural progenitor pool in the developing neocortex. Neuron 66, 386\u2013402 (2010).","journal-title":"Neuron"},{"key":"BFncomms11005_CR30","doi-asserted-by":"publisher","first-page":"e87016","DOI":"10.1371\/journal.pone.0087016","volume":"9","author":"S Kim","year":"2014","unstructured":"Kim, S. & Rhee, K. Importance of the CEP215-pericentrin interaction for centrosome maturation during mitosis. PLoS ONE 9, e87016 (2014).","journal-title":"PLoS ONE"},{"key":"BFncomms11005_CR31","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1083\/jcb.200704081","volume":"178","author":"EP Lucas","year":"2007","unstructured":"Lucas, E. P. & Raff, J. W. Maintaining the proper connection between the centrioles and the pericentriolar matrix requires Drosophila centrosomin. J. Cell Biol. 178, 725\u2013732 (2007).","journal-title":"J. Cell Biol."},{"key":"BFncomms11005_CR32","first-page":"774","volume":"9","author":"S Lee","year":"2010","unstructured":"Lee, S. & Rhee, K. CEP215 is involved in the dynein-dependent accumulation of pericentriolar matrix proteins for spindle pole formation. Cell Cycle 9, 774\u2013783 (2010).","journal-title":"Cell Cycle"},{"key":"BFncomms11005_CR33","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1038\/ng1539","volume":"37","author":"J Bond","year":"2005","unstructured":"Bond, J. et al. A centrosomal mechanism involving CDK5RAP2 and CENPJ controls brain size. Nat. Genet. 37, 353\u2013355 (2005).","journal-title":"Nat. Genet."},{"key":"BFncomms11005_CR34","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1038\/nature12517","volume":"501","author":"MA Lancaster","year":"2013","unstructured":"Lancaster, M. A. et al. Cerebral organoids model human brain development and microcephaly. Nature 501, 373\u2013379 (2013).","journal-title":"Nature"},{"key":"BFncomms11005_CR35","doi-asserted-by":"publisher","first-page":"1013","DOI":"10.1038\/nmeth968","volume":"3","author":"T Burckstummer","year":"2006","unstructured":"Burckstummer, T. et al. An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells. Nat. Methods 3, 1013\u20131019 (2006).","journal-title":"Nat. Methods"},{"key":"BFncomms11005_CR36","doi-asserted-by":"publisher","first-page":"1147","DOI":"10.1038\/ng.971","volume":"43","author":"JH Sir","year":"2011","unstructured":"Sir, J. H. et al. A primary microcephaly protein complex forms a ring around parental centrioles. Nat. Genet. 43, 1147\u20131153 (2011).","journal-title":"Nat. Genet."},{"key":"BFncomms11005_CR37","doi-asserted-by":"publisher","first-page":"e68523","DOI":"10.1371\/journal.pone.0068523","volume":"8","author":"Y Jia","year":"2013","unstructured":"Jia, Y., Fong, K. W., Choi, Y. K., See, S. S. & Qi, R. Z. Dynamic recruitment of CDK5RAP2 to centrosomes requires its association with dynein. PLoS ONE 8, e68523 (2013).","journal-title":"PLoS ONE"},{"key":"BFncomms11005_CR38","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/bs.mcb.2015.03.008","volume":"129","author":"PL Chavali","year":"2015","unstructured":"Chavali, P. L. & Gergely, F. Tackling centrosome biology through gene targeting in chicken B cells. Methods Cell Biol. 129, 83\u2013102 (2015).","journal-title":"Methods Cell Biol."},{"key":"BFncomms11005_CR39","doi-asserted-by":"crossref","first-page":"9005","DOI":"10.1016\/S0021-9258(18)52971-9","volume":"268","author":"R Chandra","year":"1993","unstructured":"Chandra, R., Salmon, E. D., Erickson, H. P., Lockhart, A. & Endow, S. A. Structural and functional domains of the Drosophila ncd microtubule motor protein. J. Biol. Chem. 268, 9005\u20139013 (1993).","journal-title":"J. Biol. Chem."},{"key":"BFncomms11005_CR40","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1038\/25153","volume":"396","author":"H Song","year":"1998","unstructured":"Song, H. & Endow, S. A. Decoupling of nucleotide- and microtubule-binding sites in a kinesin mutant. Nature 396, 587\u2013590 (1998).","journal-title":"Nature"},{"key":"BFncomms11005_CR41","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1038\/ncb1877","volume":"11","author":"G Fink","year":"2009","unstructured":"Fink, G. et al. The mitotic kinesin-14 Ncd drives directional microtubule-microtubule sliding. Nat. Cell Biol. 11, 717\u2013723 (2009).","journal-title":"Nat. Cell Biol."},{"key":"BFncomms11005_CR42","doi-asserted-by":"publisher","first-page":"4707","DOI":"10.1242\/jcs.00134","volume":"115","author":"TL Megraw","year":"2002","unstructured":"Megraw, T. L., Kilaru, S., Turner, F. R. & Kaufman, T. C. The centrosome is a dynamic structure that ejects PCM flares. J. Cell Sci. 115, 4707\u20134718 (2002).","journal-title":"J. Cell Sci."},{"key":"BFncomms11005_CR43","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1007\/s00018-014-1711-3","volume":"72","author":"MA Tollenaere","year":"2015","unstructured":"Tollenaere, M. A., Mailand, N. & Bekker-Jensen, S. Centriolar satellites: key mediators of centrosome functions. Cell Mol. Life Sci. 72, 11\u201323 (2015).","journal-title":"Cell Mol. Life Sci."},{"key":"BFncomms11005_CR44","doi-asserted-by":"publisher","first-page":"5096","DOI":"10.1242\/jcs.107326","volume":"125","author":"MA Bogoyevitch","year":"2012","unstructured":"Bogoyevitch, M. A. et al. WD40-repeat protein 62 is a JNK-phosphorylated spindle pole protein required for spindle maintenance and timely mitotic progression. J. Cell Sci. 125, 5096\u20135109 (2012).","journal-title":"J. Cell Sci."},{"key":"BFncomms11005_CR45","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1038\/ncb2269","volume":"13","author":"J Fink","year":"2011","unstructured":"Fink, J. et al. External forces control mitotic spindle positioning. Nat. Cell Biol. 13, 771\u2013778 (2011).","journal-title":"Nat. Cell Biol."},{"key":"BFncomms11005_CR46","doi-asserted-by":"publisher","first-page":"R749","DOI":"10.1016\/j.cub.2009.07.028","volume":"19","author":"S Dumont","year":"2009","unstructured":"Dumont, S. & Mitchison, T. J. Force and length in the mitotic spindle. Curr. Biol. 19, R749\u2013R761 (2009).","journal-title":"Curr. Biol."},{"key":"BFncomms11005_CR47","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1126\/science.1104905","volume":"307","author":"NJ Quintyne","year":"2005","unstructured":"Quintyne, N. J., Reing, J. E., Hoffelder, D. R., Gollin, S. M. & Saunders, W. S. Spindle multipolarity is prevented by centrosomal clustering. Science 307, 127\u2013129 (2005).","journal-title":"Science"},{"key":"BFncomms11005_CR48","doi-asserted-by":"publisher","first-page":"1399","DOI":"10.1016\/j.chembiol.2013.09.012","volume":"20","author":"CA Watts","year":"2013","unstructured":"Watts, C. A. et al. Design, synthesis, and biological evaluation of an allosteric inhibitor of HSET that targets cancer cells with supernumerary centrosomes. Chem. Biol. 20, 1399\u20131410 (2013).","journal-title":"Chem. Biol."},{"key":"BFncomms11005_CR49","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/S0074-7696(07)65003-7","volume":"265","author":"CE Walczak","year":"2008","unstructured":"Walczak, C. E. & Heald, R. Mechanisms of mitotic spindle assembly and function. Int. Rev. Cytol. 265, 111\u2013158 (2008).","journal-title":"Int. Rev. Cytol."},{"key":"BFncomms11005_CR50","doi-asserted-by":"publisher","first-page":"2805","DOI":"10.1242\/jcs.092429","volume":"125","author":"S Meunier","year":"2012","unstructured":"Meunier, S. & Vernos, I. Microtubule assembly during mitosis\u2014from distinct origins to distinct functions? J. Cell Sci. 125, 2805\u20132814 (2012).","journal-title":"J. Cell Sci."},{"key":"BFncomms11005_CR51","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1038\/nrm2609","volume":"10","author":"M Glotzer","year":"2009","unstructured":"Glotzer, M. The 3Ms of central spindle assembly: microtubules, motors and MAPs. Nat. Rev. Mol. Cell Biol. 10, 9\u201320 (2009).","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"BFncomms11005_CR52","doi-asserted-by":"publisher","first-page":"R1089","DOI":"10.1016\/j.cub.2009.10.047","volume":"19","author":"EJ Peterman","year":"2009","unstructured":"Peterman, E. J. & Scholey, J. M. Mitotic microtubule crosslinkers: insights from mechanistic studies. Curr. Biol. 19, R1089\u2013R1094 (2009).","journal-title":"Curr. Biol."},{"key":"BFncomms11005_CR53","doi-asserted-by":"publisher","first-page":"1225","DOI":"10.1091\/mbc.E14-09-1366","volume":"26","author":"S Ohta","year":"2015","unstructured":"Ohta, S. et al. CENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly. Mol. Biol. Cell 26, 1225\u20131237 (2015).","journal-title":"Mol. Biol. Cell"},{"key":"BFncomms11005_CR54","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1016\/j.cell.2010.07.047","volume":"142","author":"S Ohta","year":"2010","unstructured":"Ohta, S. et al. The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics. Cell 142, 810\u2013821 (2010).","journal-title":"Cell"},{"key":"BFncomms11005_CR55","doi-asserted-by":"publisher","first-page":"1015","DOI":"10.1038\/ng.683","volume":"42","author":"TW Yu","year":"2010","unstructured":"Yu, T. W. et al. Mutations in WDR62, encoding a centrosome-associated protein, cause microcephaly with simplified gyri and abnormal cortical architecture. Nat. Genet. 42, 1015\u20131020 (2010).","journal-title":"Nat. Genet."},{"key":"BFncomms11005_CR56","doi-asserted-by":"publisher","first-page":"1010","DOI":"10.1038\/ng.682","volume":"42","author":"AK Nicholas","year":"2010","unstructured":"Nicholas, A. K. et al. WDR62 is associated with the spindle pole and is mutated in human microcephaly. Nat. Genet. 42, 1010\u20131014 (2010).","journal-title":"Nat. Genet."},{"key":"BFncomms11005_CR57","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1083\/jcb.201401091","volume":"206","author":"MW Elting","year":"2014","unstructured":"Elting, M. W., Hueschen, C. L., Udy, D. B. & Dumont, S. Force on spindle microtubule minus ends moves chromosomes. J. Cell Biol. 206, 245\u2013256 (2014).","journal-title":"J. Cell Biol."},{"key":"BFncomms11005_CR58","doi-asserted-by":"publisher","first-page":"1907","DOI":"10.1242\/dev.040410","volume":"137","author":"SB Lizarraga","year":"2010","unstructured":"Lizarraga, S. B. et al. Cdk5rap2 regulates centrosome function and chromosome segregation in neuronal progenitors. Development 137, 1907\u20131917 (2010).","journal-title":"Development"},{"key":"BFncomms11005_CR59","doi-asserted-by":"publisher","first-page":"33ra38","DOI":"10.1126\/scitranslmed.3000915","volume":"2","author":"B Leber","year":"2010","unstructured":"Leber, B. et al. Proteins required for centrosome clustering in cancer cells. Sci. Transl. Med. 2, 33ra38 (2010).","journal-title":"Sci. Transl. Med."},{"key":"BFncomms11005_CR60","doi-asserted-by":"publisher","first-page":"3281","DOI":"10.1242\/jcs.094797","volume":"125","author":"V Marthiens","year":"2012","unstructured":"Marthiens, V., Piel, M. & Basto, R. Never tear us apart--the importance of centrosome clustering. J. Cell Sci. 125, 3281\u20133292 (2012).","journal-title":"J. Cell Sci."},{"key":"BFncomms11005_CR61","doi-asserted-by":"crossref","unstructured":"Godinho, S. A. & Pellman, D. Causes and consequences of centrosome abnormalities in cancer. Philos. Trans. R. Soc. Lond. B Biol. Sci. 369, (2014).","DOI":"10.1098\/rstb.2013.0467"},{"key":"BFncomms11005_CR62","doi-asserted-by":"publisher","first-page":"E1491","DOI":"10.1073\/pnas.1400568111","volume":"111","author":"H Bazzi","year":"2014","unstructured":"Bazzi, H. & Anderson, K. V. Acentriolar mitosis activates a p53-dependent apoptosis pathway in the mouse embryo. Proc. Natl Acad. Sci. USA 111, E1491\u2013E1500 (2014).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFncomms11005_CR63","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.1126\/science.aaa5111","volume":"348","author":"YL Wong","year":"2015","unstructured":"Wong, Y. L. et al. Cell biology. reversible centriole depletion with an inhibitor of Polo-like kinase 4. Science 348, 1155\u20131160 (2015).","journal-title":"Science"},{"key":"BFncomms11005_CR64","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1083\/jcb.201502089","volume":"210","author":"BG Lambrus","year":"2015","unstructured":"Lambrus, B. G. et al. p53 protects against genome instability following centriole duplication failure. J. Cell Biol. 210, 63\u201377 (2015).","journal-title":"J. Cell Biol."},{"key":"BFncomms11005_CR65","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/1472-6750-1-7","volume":"1","author":"H Arakawa","year":"2001","unstructured":"Arakawa, H., Lodygin, D. & Buerstedde, J. M. Mutant loxP vectors for selectable marker recycle and conditional knock-outs. BMC Biotechnol. 1, 7 (2001).","journal-title":"BMC Biotechnol."},{"key":"BFncomms11005_CR66","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1083\/jcb.201106025","volume":"195","author":"D Zyss","year":"2011","unstructured":"Zyss, D., Ebrahimi, H. & Gergely, F. Casein kinase I delta controls centrosome positioning during T cell activation. J. Cell Biol. 195, 781\u2013797 (2011).","journal-title":"J. Cell Biol."},{"key":"BFncomms11005_CR67","doi-asserted-by":"publisher","first-page":"e1003657","DOI":"10.1371\/journal.pcbi.1003657","volume":"10","author":"M Kuhn","year":"2014","unstructured":"Kuhn, M., Hyman, A. A. & Beyer, A. Coiled-coil proteins facilitated the functional expansion of the centrosome. PLoS Comput. Biol. 10, e1003657 (2014).","journal-title":"PLoS Comput. Biol."},{"key":"BFncomms11005_CR68","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1038\/emboj.2009.331","volume":"29","author":"CS Fong","year":"2010","unstructured":"Fong, C. S., Sato, M. & Toda, T. Fission yeast Pcp1 links polo kinase-mediated mitotic entry to gamma-tubulin-dependent spindle formation. EMBO J. 29, 120\u2013130 (2010).","journal-title":"EMBO J."},{"key":"BFncomms11005_CR69","doi-asserted-by":"publisher","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","volume":"23","author":"MA Larkin","year":"2007","unstructured":"Larkin, M. A. et al. Clustal W and Clustal X version 2.0. Bioinformatics 23, 2947\u20132948 (2007).","journal-title":"Bioinformatics"},{"key":"BFncomms11005_CR70","doi-asserted-by":"publisher","first-page":"D1063","DOI":"10.1093\/nar\/gks1262","volume":"41","author":"JA Vizcaino","year":"2013","unstructured":"Vizcaino, J. A. et al. The PRoteomics IDEntifications (PRIDE) database and associated tools: status in 2013. Nucleic Acids Res. 41, D1063\u2013D1069 (2013).","journal-title":"Nucleic Acids Res."}],"container-title":["Nature Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/ncomms11005.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/ncomms11005","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/ncomms11005.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T11:44:02Z","timestamp":1672832642000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/ncomms11005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,18]]},"references-count":70,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,4,22]]}},"alternative-id":["BFncomms11005"],"URL":"https:\/\/doi.org\/10.1038\/ncomms11005","relation":{},"ISSN":["2041-1723"],"issn-type":[{"value":"2041-1723","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,18]]},"assertion":[{"value":"10 September 2015","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 February 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 March 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"11005"}}