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Here we report the first retromer-trafficking interactome in<jats:italic>Toxoplasma gondii.<\/jats:italic>This retromer complex includes a trimer Vps35\u2013Vps26\u2013Vps29 core complex that serves as a hub for the endosome-like compartment and parasite-specific proteins. Conditional ablation of<jats:italic>Tg<\/jats:italic>Vps35 reveals that the retromer complex is crucial for the biogenesis of secretory organelles and for maintaining parasite morphology. We identify<jats:italic>Tg<\/jats:italic>HP12 as a parasite-specific and retromer-associated protein with functions unrelated to secretory organelle formation. Furthermore, the major facilitator superfamily homologue named<jats:italic>Tg<\/jats:italic>HP03, which is a multiple spanning and ligand transmembrane transporter, is maintained at the parasite membrane by retromer-mediated endocytic recycling. Thus, our findings highlight that both evolutionarily conserved and unconventional proteins act in concert in<jats:italic>T. gondii<\/jats:italic>by controlling retrograde transport that is essential for parasite integrity and host infection.<\/jats:p>","DOI":"10.1038\/ncomms11191","type":"journal-article","created":{"date-parts":[[2016,4,11]],"date-time":"2016-04-11T09:11:41Z","timestamp":1460365901000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":83,"title":["Unconventional endosome-like compartment and retromer complex in Toxoplasma gondii govern parasite integrity and host infection"],"prefix":"10.1038","volume":"7","author":[{"given":"Lamba Omar","family":"Sangar\u00e9","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tchilabalo Dilezitoko","family":"Alayi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit","family":"Westermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnes","family":"Hovasse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabien","family":"Sindikubwabo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Callebaut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Werkmeister","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Lafont","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Slomianny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed-Ali","family":"Hakimi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Van Dorsselaer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Schaeffer-Reiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanislas","family":"Tomavo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,4,11]]},"reference":[{"key":"BFncomms11191_CR1","doi-asserted-by":"publisher","first-page":"1385","DOI":"10.1016\/j.ijpara.2009.04.003","volume":"39","author":"G Pappas","year":"2009","unstructured":"Pappas, G., Roussos, N. & Falagas, M. 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