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Here, we reconstitute the noscapine gene cluster in\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    to achieve the microbial production of noscapine and related pathway intermediates, complementing and extending previous\n                    <jats:italic>in planta<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    investigations. Our work provides structural validation of the secoberberine intermediates and the description of the narcotoline-4\u2032-O-methyltransferase, suggesting this activity is catalysed by a unique heterodimer. We also reconstitute a 14-step biosynthetic pathway of noscapine from the simple alkaloid norlaudanosoline by engineering a yeast strain expressing 16 heterologous plant enzymes, achieving reconstitution of a complex plant pathway in a microbial host. Other engineered yeasts produce previously inaccessible pathway intermediates and a novel derivative, thereby advancing protoberberine and noscapine related drug discovery.\n                  <\/jats:p>","DOI":"10.1038\/ncomms12137","type":"journal-article","created":{"date-parts":[[2016,7,5]],"date-time":"2016-07-05T07:21:41Z","timestamp":1467703301000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":131,"title":["Engineering biosynthesis of the anticancer alkaloid noscapine in yeast"],"prefix":"10.1038","volume":"7","author":[{"given":"Yanran","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina D.","family":"Smolke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,7,5]]},"reference":[{"key":"BFncomms12137_CR1","doi-asserted-by":"crossref","unstructured":"Osbourn, A. 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