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Decreased cellular responses in WASp-deficient cells have been interpreted to mean that WASp directly regulates these responses in WASp-sufficient cells. Here, we identify an exception to this concept and show that WASp-deficient dendritic cells have increased activation of Rac2 that support cross-presentation to CD8<jats:sup>+<\/jats:sup> T cells. Using two different skin pathology models, WASp-deficient mice show an accumulation of dendritic cells in the skin and increased expansion of IFN\u03b3-producing CD8<jats:sup>+<\/jats:sup> T cells in the draining lymph node and spleen. Specific deletion of WASp in dendritic cells leads to marked expansion of CD8<jats:sup>+<\/jats:sup> T cells at the expense of CD4<jats:sup>+<\/jats:sup> T cells. WASp-deficient dendritic cells induce increased cross-presentation to CD8<jats:sup>+<\/jats:sup> T cells by activating Rac2 that maintains a near neutral pH of phagosomes. Our data reveals an intricate balance between activation of WASp and Rac2 signalling pathways in dendritic cells.<\/jats:p>","DOI":"10.1038\/ncomms12175","type":"journal-article","created":{"date-parts":[[2016,7,18]],"date-time":"2016-07-18T09:37:24Z","timestamp":1468834644000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Deletion of Wiskott\u2013Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells"],"prefix":"10.1038","volume":"7","author":[{"given":"Marisa A. P.","family":"Baptista","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marton","family":"Keszei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen K. 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