{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T04:17:27Z","timestamp":1792297047074,"version":"4.4.0"},"reference-count":56,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,12,2]],"date-time":"2016-12-02T00:00:00Z","timestamp":1480636800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,12,2]],"date-time":"2016-12-02T00:00:00Z","timestamp":1480636800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Commun"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Mounting\n                    <jats:italic>in vitro<\/jats:italic>\n                    ,\n                    <jats:italic>in vivo<\/jats:italic>\n                    and clinical evidence suggest an important role for filopodia in driving cancer cell invasion. Using a high-throughput microscopic-based drug screen, we identify FDA-approved calcium channel blockers (CCBs) as potent inhibitors of filopodia formation in cancer cells. Unexpectedly, we discover that L-type calcium channels are functional and frequently expressed in cancer cells suggesting a previously unappreciated role for these channels during tumorigenesis. We further demonstrate that, at filopodia, L-type calcium channels are activated by integrin inside-out signalling, integrin activation and Src. Moreover, L-type calcium channels promote filopodia stability and maturation into talin-rich adhesions through the spatially restricted regulation of calcium entry and subsequent activation of the protease calpain-1. Altogether we uncover a novel and clinically relevant signalling pathway that regulates filopodia formation in cancer cells and propose that cycles of filopodia stabilization, followed by maturation into focal adhesions, directs cancer cell migration and invasion.\n                  <\/jats:p>","DOI":"10.1038\/ncomms13297","type":"journal-article","created":{"date-parts":[[2016,12,2]],"date-time":"2016-12-02T05:23:07Z","timestamp":1480656187000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":184,"title":["L-type calcium channels regulate filopodia stability and cancer cell invasion downstream of integrin signalling"],"prefix":"10.1038","volume":"7","author":[{"given":"Guillaume","family":"Jacquemet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Habib","family":"Baghirov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Georgiadou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5265-5509","authenticated-orcid":false,"given":"Harri","family":"Sihto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilia","family":"Peuhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Cettour-Janet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merja","family":"Per\u00e4l\u00e4","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauliina","family":"Kronqvist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heikki","family":"Joensuu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6295-6556","authenticated-orcid":false,"given":"Johanna","family":"Ivaska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,12,2]]},"reference":[{"key":"BFncomms13297_CR1","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1038\/nature14971","volume":"525","author":"B Waclaw","year":"2015","unstructured":"Waclaw, B. et al. A spatial model predicts that dispersal and cell turnover limit intratumour heterogeneity. Nature 525, 261\u2013264 (2015).","journal-title":"Nature"},{"key":"BFncomms13297_CR2","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1038\/nature14347","volume":"520","author":"G Gundem","year":"2015","unstructured":"Gundem, G. et al. The evolutionary history of lethal metastatic prostate cancer. Nature 520, 353\u2013357 (2015).","journal-title":"Nature"},{"key":"BFncomms13297_CR3","doi-asserted-by":"publisher","first-page":"679","DOI":"10.1016\/j.cell.2006.11.001","volume":"127","author":"GP Gupta","year":"2006","unstructured":"Gupta, G. P. & Massagu\u00e9, J. Cancer Metastasis: Building a Framework. Cell 127, 679\u2013695 (2006).","journal-title":"Cell"},{"key":"BFncomms13297_CR4","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1101\/gad.1758709","volume":"23","author":"KR Legate","year":"2009","unstructured":"Legate, K. R., Wickstr\u00f6m, S. A. & F\u00e4ssler, R. Genetic and cell biological analysis of integrin outside-in signaling. Genes Dev. 23, 397\u2013418 (2009).","journal-title":"Genes Dev."},{"key":"BFncomms13297_CR5","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1242\/jcs.018556","volume":"122","author":"JA Askari","year":"2009","unstructured":"Askari, J. A., Buckley, P. A., Mould, A. P. & Humphries, M. J. Linking integrin conformation to function. J. Cell Sci. 122, 165\u2013170 (2009).","journal-title":"J. Cell Sci."},{"key":"BFncomms13297_CR6","doi-asserted-by":"publisher","first-page":"627","DOI":"10.1016\/j.ceb.2013.05.008","volume":"25","author":"G Jacquemet","year":"2013","unstructured":"Jacquemet, G., Humphries, M. J. & Caswell, P. T. Role of adhesion receptor trafficking in 3D cell migration. Curr. Opin. Cell Biol. 25, 627\u2013632 (2013).","journal-title":"Curr. Opin. Cell Biol."},{"key":"BFncomms13297_CR7","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1016\/S0092-8674(02)00971-6","volume":"110","author":"RO Hynes","year":"2002","unstructured":"Hynes, R. O. Integrins: bidirectional, allosteric signaling machines in their roles as major adhesion receptors, integrins. Cell 110, 673\u2013687 (2002).","journal-title":"Cell"},{"key":"BFncomms13297_CR8","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.ceb.2015.06.007","volume":"36","author":"G Jacquemet","year":"2015","unstructured":"Jacquemet, G., Hamidi, H. & Ivaska, J. Filopodia in cell adhesion, 3D migration and cancer cell invasion. Curr. Opin. Cell Biol. 36, 23\u201331 (2015).","journal-title":"Curr. Opin. Cell Biol."},{"key":"BFncomms13297_CR9","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1083\/jcb.201302041","volume":"202","author":"G Jacquemet","year":"2013","unstructured":"Jacquemet, G. et al. RCP-driven \u03b15\u03b21 recycling suppresses Rac and promotes RhoA activity via the RacGAP1-IQGAP1 complex. J. Cell Biol. 202, 917\u2013935 (2013).","journal-title":"J. Cell Biol."},{"key":"BFncomms13297_CR10","doi-asserted-by":"publisher","first-page":"1013","DOI":"10.1083\/jcb.201502040","volume":"210","author":"NR Paul","year":"2015","unstructured":"Paul, N. R. et al. \u03b15\u03b21 integrin recycling promotes Arp2\/3-independent cancer cell invasion via the formin FHOD3. J. Cell Biol. 210, 1013\u20131031 (2015).","journal-title":"J. Cell Biol."},{"key":"BFncomms13297_CR11","doi-asserted-by":"publisher","first-page":"706","DOI":"10.1158\/2159-8290.CD-11-0239","volume":"2","author":"T Shibue","year":"2012","unstructured":"Shibue, T., Brooks, M. W., Inan, M. F., Reinhardt, F. & Weinberg, R. A. The outgrowth of micrometastases is enabled by the formation of filopodium-like protrusions. Cancer Discov. 2, 706\u2013721 (2012).","journal-title":"Cancer Discov."},{"key":"BFncomms13297_CR12","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1016\/j.ccr.2013.08.012","volume":"24","author":"T Shibue","year":"2013","unstructured":"Shibue, T., Brooks, M. W. & Weinberg, R. A. An integrin-linked machinery of cytoskeletal regulation that enables experimental tumor initiation and metastatic colonization. Cancer Cell 24, 481\u2013498 (2013).","journal-title":"Cancer Cell"},{"key":"BFncomms13297_CR13","doi-asserted-by":"publisher","first-page":"1069","DOI":"10.1172\/JCI67280","volume":"124","author":"A Arjonen","year":"2014","unstructured":"Arjonen, A. et al. Mutant p53-associated myosin-X upregulation promotes breast cancer invasion and metastasis. J. Clin. Invest. 124, 1069\u20131082 (2014).","journal-title":"J. Clin. Invest."},{"key":"BFncomms13297_CR14","doi-asserted-by":"publisher","first-page":"1386","DOI":"10.1053\/j.gastro.2014.01.046","volume":"146","author":"A Li","year":"2014","unstructured":"Li, A. et al. Fascin is regulated by slug, promotes progression of pancreatic cancer in mice, and is associated with patient outcomes. Gastroenterology 146, 1386 (2014).","journal-title":"Gastroenterology"},{"key":"BFncomms13297_CR15","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1038\/bjc.2014.298","volume":"111","author":"R Cao","year":"2014","unstructured":"Cao, R. et al. Elevated expression of myosin X in tumours contributes to breast cancer aggressiveness and metastasis. Br. J. Cancer 111, 539\u2013550 (2014).","journal-title":"Br. J. Cancer"},{"key":"BFncomms13297_CR16","doi-asserted-by":"publisher","first-page":"3525","DOI":"10.1242\/jcs.069609","volume":"123","author":"L Plantard","year":"2010","unstructured":"Plantard, L. et al. PtdIns(3,4,5)P3 is a regulator of myosin-X localization and filopodia formation. J. Cell Sci. 123, 3525\u20133534 (2010).","journal-title":"J. Cell Sci."},{"key":"BFncomms13297_CR17","doi-asserted-by":"publisher","first-page":"a003947","DOI":"10.1101\/cshperspect.a003947","volume":"3","author":"WA Catterall","year":"2011","unstructured":"Catterall, W. A. Voltage-gated calcium channels. Cold Spring Harb. Perspect. Biol. 3, a003947 (2011).","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"BFncomms13297_CR18","doi-asserted-by":"publisher","first-page":"2633","DOI":"10.1152\/jn.00486.2004","volume":"92","author":"D Lipscombe","year":"2004","unstructured":"Lipscombe, D., Helton, T. D. & Xu, W. L-type calcium channels: the low down. J. Neurophysiol. 92, 2633\u20132641 (2004).","journal-title":"J. Neurophysiol."},{"key":"BFncomms13297_CR19","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1002\/wmts.91","volume":"2","author":"JR Tyson","year":"2013","unstructured":"Tyson, J. R. & Snutch, T. P. Molecular nature of voltage-gated calcium channels: structure and species comparison. WIREs Membr. Transp. Signal 2, 181\u2013206 (2013).","journal-title":"WIREs Membr. Transp. Signal"},{"key":"BFncomms13297_CR20","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1038\/nbt.3080","volume":"33","author":"C Klijn","year":"2015","unstructured":"Klijn, C. et al. A comprehensive transcriptional portrait of human cancer cell lines. Nat. Biotechnol. 33, 306\u2013312 (2015).","journal-title":"Nat. Biotechnol."},{"key":"BFncomms13297_CR21","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1038\/nature12354","volume":"499","author":"T-W Chen","year":"2013","unstructured":"Chen, T.-W. et al. Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature 499, 295\u2013300 (2013).","journal-title":"Nature"},{"key":"BFncomms13297_CR22","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1158\/2159-8290.CD-12-0095","volume":"2","author":"E Cerami","year":"2012","unstructured":"Cerami, E. et al. The cBio cancer genomics portal: an open platform for exploring multidimensional cancer genomics data. Cancer Discov. 2, 401\u2013404 (2012).","journal-title":"Cancer Discov."},{"key":"BFncomms13297_CR23","doi-asserted-by":"publisher","first-page":"pl1","DOI":"10.1126\/scisignal.2004088","volume":"6","author":"J Gao","year":"2013","unstructured":"Gao, J. et al. Integrative analysis of complex cancer genomics and clinical profiles using the cBioPortal. Sci. Signal. 6, pl1 (2013).","journal-title":"Sci. Signal."},{"key":"BFncomms13297_CR24","doi-asserted-by":"publisher","first-page":"506","DOI":"10.1016\/j.cell.2015.09.033","volume":"163","author":"G Ciriello","year":"2015","unstructured":"Ciriello, G. et al. Comprehensive molecular portraits of invasive lobular breast cancer. Cell 163, 506\u2013519 (2015).","journal-title":"Cell"},{"key":"BFncomms13297_CR25","doi-asserted-by":"publisher","first-page":"e0125766","DOI":"10.1371\/journal.pone.0125766","volume":"10","author":"C-Y Wang","year":"2015","unstructured":"Wang, C.-Y., Lai, M.-D., Phan, N. N., Sun, Z. & Lin, Y.-C. Meta-analysis of public microarray datasets reveals voltage-gated calcium gene signatures in clinical cancer patients. PLoS ONE 10, e0125766 (2015).","journal-title":"PLoS ONE"},{"key":"BFncomms13297_CR26","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1038\/ncb1136","volume":"6","author":"H Zhang","year":"2004","unstructured":"Zhang, H. et al. Myosin-X provides a motor-based link between integrins and the cytoskeleton. Nat. Cell Biol. 6, 523\u2013531 (2004).","journal-title":"Nat. Cell Biol."},{"key":"BFncomms13297_CR27","doi-asserted-by":"publisher","first-page":"8492","DOI":"10.1038\/ncomms9492","volume":"6","author":"F Lagarrigue","year":"2015","unstructured":"Lagarrigue, F. et al. A RIAM\/lamellipodin-talin-integrin complex forms the tip of sticky fingers that guide cell migration. Nat. Commun. 6, 8492 (2015).","journal-title":"Nat. Commun."},{"key":"BFncomms13297_CR28","doi-asserted-by":"publisher","first-page":"28071","DOI":"10.1074\/jbc.274.40.28071","volume":"274","author":"DA Calderwood","year":"1999","unstructured":"Calderwood, D. A. et al. The Talin head domain binds to integrin beta subunit cytoplasmic tails and regulates integrin activation. J. Biol. Chem. 274, 28071\u201328074 (1999).","journal-title":"J. Biol. Chem."},{"key":"BFncomms13297_CR29","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/j.ceb.2005.02.009","volume":"17","author":"JL Bos","year":"2005","unstructured":"Bos, J. L. Linking Rap to cell adhesion. Curr. Opin. Cell Biol. 17, 123\u2013128 (2005).","journal-title":"Curr. Opin. Cell Biol."},{"key":"BFncomms13297_CR30","doi-asserted-by":"publisher","first-page":"4009","DOI":"10.1242\/jcs.056770","volume":"122","author":"A Byron","year":"2009","unstructured":"Byron, A. et al. Anti-integrin monoclonal antibodies. J. Cell Sci. 122, 4009\u20134011 (2009).","journal-title":"J. Cell Sci."},{"key":"BFncomms13297_CR31","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1016\/j.critrevonc.2013.12.009","volume":"89","author":"B Elsberger","year":"2014","unstructured":"Elsberger, B. Translational evidence on the role of Src kinase and activated Src kinase in invasive breast cancer. Crit. Rev. Oncol. Hematol. 89, 343\u2013351 (2014).","journal-title":"Crit. Rev. Oncol. Hematol."},{"key":"BFncomms13297_CR32","doi-asserted-by":"publisher","first-page":"987","DOI":"10.1016\/j.ajpath.2014.12.018","volume":"185","author":"B Vassilev","year":"2015","unstructured":"Vassilev, B. et al. Elevated levels of StAR-related lipid transfer protein 3 alter cholesterol balance and adhesiveness of breast cancer cells: potential mechanisms contributing to progression of HER2-positive breast cancers. Am. J. Pathol. 185, 987\u20131000 (2015).","journal-title":"Am. J. Pathol."},{"key":"BFncomms13297_CR33","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/j.jmb.2004.11.039","volume":"346","author":"D Stockholm","year":"2005","unstructured":"Stockholm, D. et al. Imaging calpain protease activity by multiphoton FRET in living mice. J. Mol. Biol. 346, 215\u2013222 (2005).","journal-title":"J. Mol. Biol."},{"key":"BFncomms13297_CR34","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1083\/jcb.201406102","volume":"208","author":"HE Johnson","year":"2015","unstructured":"Johnson, H. E. et al. F-actin bundles direct the initiation and orientation of lamellipodia through adhesion-based signaling. J. Cell Biol. 208, 443\u2013455 (2015).","journal-title":"J. Cell Biol."},{"key":"BFncomms13297_CR35","doi-asserted-by":"publisher","first-page":"e107097","DOI":"10.1371\/journal.pone.0107097","volume":"9","author":"W Hu","year":"2014","unstructured":"Hu, W., Wehrle-Haller, B. & Vogel, V. Maturation of filopodia shaft adhesions is upregulated by local cycles of lamellipodia advancements and retractions. PLoS ONE 9, e107097 (2014).","journal-title":"PLoS ONE"},{"key":"BFncomms13297_CR36","doi-asserted-by":"publisher","first-page":"2883","DOI":"10.1096\/fj.14-265603","volume":"29","author":"J Hao","year":"2015","unstructured":"Hao, J. et al. Ca2+ channel subunit \u03b1 1D promotes proliferation and migration of endometrial cancer cells mediated by 17\u03b2-estradiol via the G protein-coupled estrogen receptor. FASEB J. 29, 2883\u20132893 (2015).","journal-title":"FASEB J."},{"key":"BFncomms13297_CR37","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1016\/j.urolonc.2013.05.011","volume":"32","author":"R Chen","year":"2014","unstructured":"Chen, R. et al. Cav1.3 channel \u03b11D protein is overexpressed and modulates androgen receptor transactivation in prostate cancers. Urol. Oncol. 32, 524\u2013536 (2014).","journal-title":"Urol. Oncol."},{"key":"BFncomms13297_CR38","doi-asserted-by":"publisher","first-page":"27130","DOI":"10.1074\/jbc.M501625200","volume":"280","author":"S Yang","year":"2005","unstructured":"Yang, S. & Huang, X.-Y. Ca2+ influx through L-type Ca2+ channels controls the trailing tail contraction in growth factor-induced fibroblast cell migration. J. Biol. Chem. 280, 27130\u201327137 (2005).","journal-title":"J. Biol. Chem."},{"key":"BFncomms13297_CR39","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1083\/jcb.143.1.241","volume":"143","author":"X Wu","year":"1998","unstructured":"Wu, X. et al. Modulation of calcium current in arteriolar smooth muscle by \u03b1v\u03b23 and \u03b15\u03b21 integrin ligands. J. Cell Biol. 143, 241\u2013252 (1998).","journal-title":"J. Cell Biol."},{"key":"BFncomms13297_CR40","doi-asserted-by":"publisher","first-page":"30285","DOI":"10.1074\/jbc.M102436200","volume":"276","author":"X Wu","year":"2001","unstructured":"Wu, X., Davis, G. E., Meininger, G. A., Wilson, E. & Davis, M. J. Regulation of the L-type calcium channel by alpha 5beta 1 integrin requires signaling between focal adhesion proteins. J. Biol. Chem. 276, 30285\u201330292 (2001).","journal-title":"J. Biol. Chem."},{"key":"BFncomms13297_CR41","doi-asserted-by":"publisher","first-page":"14015","DOI":"10.1074\/jbc.M600433200","volume":"281","author":"P Gui","year":"2006","unstructured":"Gui, P. et al. Integrin receptor activation triggers converging regulation of Cav1.2 calcium channels by c-Src and protein kinase A pathways. J. Biol. Chem. 281, 14015\u201314025 (2006).","journal-title":"J. Biol. Chem."},{"key":"BFncomms13297_CR42","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1016\/S0896-6273(03)00260-5","volume":"38","author":"E Robles","year":"2003","unstructured":"Robles, E., Huttenlocher, A. & Gomez, T. M. Filopodial calcium transients regulate growth cone motility and guidance through local activation of calpain. Neuron 38, 597\u2013609 (2003).","journal-title":"Neuron"},{"key":"BFncomms13297_CR43","doi-asserted-by":"publisher","first-page":"1983","DOI":"10.1126\/science.1056490","volume":"291","author":"TM Gomez","year":"2001","unstructured":"Gomez, T. M., Robles, E., Poo, M. & Spitzer, N. C. Filopodial calcium transients promote substrate-dependent growth cone turning. Science 291, 1983\u20131987 (2001).","journal-title":"Science"},{"key":"BFncomms13297_CR44","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1523\/JNEUROSCI.20-01-00315.2000","volume":"20","author":"NJ Lautermilch","year":"2000","unstructured":"Lautermilch, N. J. & Spitzer, N. C. Regulation of calcineurin by growth cone calcium waves controls neurite extension. J. Neurosci. 20, 315\u2013325 (2000).","journal-title":"J. Neurosci."},{"key":"BFncomms13297_CR45","doi-asserted-by":"publisher","first-page":"835","DOI":"10.1016\/j.neuron.2004.08.037","volume":"43","author":"Z Wen","year":"2004","unstructured":"Wen, Z., Guirland, C., Ming, G.-L. & Zheng, J. Q. A CaMKII\/calcineurin switch controls the direction of Ca(2+)-dependent growth cone guidance. Neuron 43, 835\u2013846 (2004).","journal-title":"Neuron"},{"key":"BFncomms13297_CR46","doi-asserted-by":"publisher","first-page":"185","DOI":"10.3389\/fphys.2013.00185","volume":"4","author":"M Yang","year":"2013","unstructured":"Yang, M. & Brackenbury, W. J. Membrane potential and cancer progression. Front. Physiol. 4, 185 (2013).","journal-title":"Front. Physiol."},{"key":"BFncomms13297_CR47","doi-asserted-by":"publisher","first-page":"5381","DOI":"10.1158\/1078-0432.CCR-05-0327","volume":"11","author":"SP Fraser","year":"2005","unstructured":"Fraser, S. P. et al. Voltage-gated sodium channel expression and potentiation of human breast cancer metastasis. Clin. Cancer Res. 11, 5381\u20135389 (2005).","journal-title":"Clin. Cancer Res."},{"key":"BFncomms13297_CR48","doi-asserted-by":"publisher","first-page":"712","DOI":"10.1016\/j.cell.2015.09.053","volume":"163","author":"MY Hein","year":"2015","unstructured":"Hein, M. Y. et al. A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 163, 712\u2013723 (2015).","journal-title":"Cell"},{"key":"BFncomms13297_CR49","doi-asserted-by":"publisher","first-page":"R1092","DOI":"10.1016\/j.cub.2015.09.049","volume":"25","author":"NR Paul","year":"2015","unstructured":"Paul, N. R., Jacquemet, G. & Caswell, P. T. Endocytic trafficking of integrins in cell migration. Curr. Biol. 25, R1092\u2013R1105 (2015).","journal-title":"Curr. Biol."},{"key":"BFncomms13297_CR50","doi-asserted-by":"publisher","first-page":"6135","DOI":"10.1038\/ncomms7135","volume":"6","author":"A Byron","year":"2015","unstructured":"Byron, A. et al. A proteomic approach reveals integrin activation state-dependent control of microtubule cortical targeting. Nat. Commun. 6, 6135 (2015).","journal-title":"Nat. Commun."},{"key":"BFncomms13297_CR51","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.jsb.2005.06.002","volume":"151","author":"IF Sbalzarini","year":"2005","unstructured":"Sbalzarini, I. F. & Koumoutsakos, P. Feature point tracking and trajectory analysis for video imaging in cell biology. J. Struct. Biol. 151, 182\u2013195 (2005).","journal-title":"J. Struct. Biol."},{"key":"BFncomms13297_CR52","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1083\/jcb.201306067","volume":"204","author":"D Tsygankov","year":"2014","unstructured":"Tsygankov, D. et al. CellGeo: a computational platform for the analysis of shape changes in cells with complex geometries. J. Cell Biol. 204, 443\u2013460 (2014).","journal-title":"J. Cell Biol."},{"key":"BFncomms13297_CR53","doi-asserted-by":"publisher","first-page":"e22025","DOI":"10.1371\/journal.pone.0022025","volume":"6","author":"ME Berginski","year":"2011","unstructured":"Berginski, M. E., Vitriol, E. A., Hahn, K. M. & Gomez, S. M. High-resolution quantification of focal adhesion spatiotemporal dynamics in living cells. PLoS ONE 6, e22025 (2011).","journal-title":"PLoS ONE"},{"key":"BFncomms13297_CR54","doi-asserted-by":"publisher","first-page":"4121","DOI":"10.1242\/jcs.121988","volume":"126","author":"G Jacquemet","year":"2013","unstructured":"Jacquemet, G. et al. Rac1 is deactivated at integrin activation sites through an IQGAP1-filamin-A-RacGAP1 pathway. J. Cell Sci. 126, 4121\u20134135 (2013).","journal-title":"J. Cell Sci."},{"key":"BFncomms13297_CR55","first-page":"3591","volume":"9","author":"RF Hennigan","year":"1994","unstructured":"Hennigan, R. F., Hawker, K. L. & Ozanne, B. W. Fos-transformation activates genes associated with invasion. Oncogene 9, 3591\u20133600 (1994).","journal-title":"Oncogene"},{"key":"BFncomms13297_CR56","first-page":"923","volume":"9","author":"H Joensuu","year":"2003","unstructured":"Joensuu, H. et al. Amplification of erbB2 and erbB2 expression are superior to estrogen receptor status as risk factors for distant recurrence in pT1N0M0 breast cancer: a nationwide population-based study. Clin. Cancer Res. 9, 923\u2013930 (2003).","journal-title":"Clin. Cancer Res."}],"container-title":["Nature Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/ncomms13297.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/ncomms13297","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/ncomms13297.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T04:46:35Z","timestamp":1672807595000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/ncomms13297"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,2]]},"references-count":56,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,12,23]]}},"alternative-id":["BFncomms13297"],"URL":"https:\/\/doi.org\/10.1038\/ncomms13297","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.727060770.793526195","asserted-by":"object"}]},"ISSN":["2041-1723"],"issn-type":[{"value":"2041-1723","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,2]]},"assertion":[{"value":"7 March 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 September 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 December 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"13297"}}